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Search Results: 1 - 10 of 259053 matches for " Lúcia Gracinda da;Boddey "
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Quantification of natural populations of Gluconacetobacter diazotrophicus and Herbaspirillum spp. In sugar cane (Saccharum spp.) Using differente polyclonal antibodies
Silva-Froufe, Lúcia Gracinda da;Boddey, Robert Michael;Reis, Veronica Massena;
Brazilian Journal of Microbiology , 2009, DOI: 10.1590/S1517-83822009000400018
Abstract: the species gluconacetobacterdiazotrophicus, herbaspirillum seropedicae and h. rubrisubalbicans are endophytic n2-fixing [diazotrophic] bacteria which colonise not only roots, but also the aerial tissue of sugar cane. however, the technique most commonly used to quantify the populations of these microbes in plants is by culturing serial dilutions of macerates of plant tissues in n free semi-solid media which are only semi-selective for the species/genera [the most probable number (mpn) technique] and each culture must be further subjected to several tests to identify the isolates at the species level. the use of species-specific polyclonal antibodies with the indirect elisa (enzyme-linked immunosorbent assay) can be an alternative which is rapid and specific to quantify these populations of bacteria. this study was performed to investigate the viability of adapting the indirect elisa technique to quantify individually the populations of these three species of diazotroph within the root and shoot tissues of sugarcane. the results showed that species-specific polyclonal antibodies could be obtained by purifying sera in protein-a columns which removed non-specific immuno-globulins. it was possible to quantify the three bacterial species in the brazilian sugarcane variety sp 70-1143 in numbers above 105 cells per g fresh weight in roots, rhizomes and leaves. the numbers of the different bacterial species evaluated using the elisa technique were found to be higher than when the same populations were evaluated using the mpn technique, reaching 1400 times greater for g. diazotrophicus and 225 times greater for herbaspirillum spp. these results constitute the first quantification of herbaspirillum using immunological techniques.
Herbaspirillum seropedicae and sugarcane endophytic interaction investigated by using high pressure freezing electron microscopy
Silva, Lúcia Gracinda da;Miguens, Flávio Costa;Olivares, Fabio Lopes;
Brazilian Journal of Microbiology , 2003, DOI: 10.1590/S1517-83822003000500023
Abstract: the interaction between sugar cane plantlets and h. seropedicae was investigated using high pressure freezing followed by freeze substitution. microscopical observation showed consistent differences between this approaches when compared with the conventional preparation, specially related to appearance of the bacteria cell and the endophytic attachment to the host cell wall.
Promo??o de enraizamento de microtoletes de cana-de-a?úcar pelo uso conjunto de substancias húmicas e bactérias diazotróficas endofíticas
Marques Júnior, Roberto Batista;Canellas, Luciano Pasqualoto;Silva, Lúcia Gracinda da;Olivares, Fábio Lopes;
Revista Brasileira de Ciência do Solo , 2008, DOI: 10.1590/S0100-06832008000300020
Abstract: besides the direct nutritional effect of mineralization of organic matter or by biological nitrogen fixation activity, the humic substances and endophytic diazotrophic bacteria can directly influence plant metabolism, modifying plant growth and development patterns. the purpose of this study was to evaluate the effect of the combined use of humic acid (ha) and herbaspirillum seropedicae, an endophytic nitrogen-fixing bacteria, on the root growth of seed pieces - heat-treated or not - of the sugarcane variety rb 72 454. after hot water treatment, the cane cuttings were immersed for 12 hours as follows: in water (control plant), in ha solution (20 mg l-1 of c) from vermicompost, in bacterial inoculant of h. seropedicae, strain hrc54 (108 cells ml-1), and in a combination of bacteria and ha. root growth was improved by 60 to 118 % in length and from 33 % to 233 % in surface area on sugarcane plant treatments compared to control, with more pronounced effect in plants under heat treatment. likewise, the positive effect of the combinded treatment (bacteria inoculation and humic acid) on shoot and root biomass was significant compared to the control with heat-treated cuts. for non-heated seed pieces, bacteria inoculation did not result in a positive plant growth effect, but only in the presence of humic acid. the combined or isolated use of both ha and bacteria did not significantly modify the bacteria population in the root tissue of heat-untreated sugarcane. for heat-treated cuts, bacteria inoculation, combined or not with ha, increased the size of diazotrophic bacteria population on roots. the results highlight the importance of thermotherapy to increase the positive effect of the selected bacteria strain of h. seropedicae. in the case of ha application, plant growth stimulation was not related to thermotherapy. the plant root growth promoting effect induced by both ha and the inoculation of selected nitrogen-fixing bacteria strain could represent an innovative approach
Endotracheal and upper airways suctioning: changes in newborns? physiological parameters
Barbosa, Andréa Lopes;Cardoso, Maria Vera Lúcia Moreira Leit?o;Brasil, Thays Bezerra;Scochi, Carmen Gracinda Silvan;
Revista Latino-Americana de Enfermagem , 2011, DOI: 10.1590/S0104-11692011000600013
Abstract: this study investigated which physiological parameters change when endotracheal and upper airway suctioning is performed immediately before, immediately after and five minutes after this procedure is performed in newborns hospitalized in a neonatal intensive care unit (nicu). this is a quantitative and longitudinal study, before and after type, performed in the nicu of a public institution in the city of fortaleza, ce, brazil. the sample was composed of 104 newborns using oxigenotherapy and who needed endotracheal and upper airway suctioning. the results showed significant alterations in respiratory and heart rates (p<0.05) in neonates using oxyhood and nasal cpap while the pulse significantly changed (p<0.05) in newborns placed in oxyhood, using nasal cpap and mechanical ventilation; oxygen saturation was the only parameter that did not alter significantly. we propose that nurses develop non-pharmacological interventions to reduce potential alterations caused in newborns’ physiological parameters due to this procedure.
Dinamica da serapilheira em pastagens de braquiária em sistema silvipastoril e monocultura
Xavier, Deise Ferreira;Lédo, Francisco José da Silva;Paciullo, Domingos Sávio de Campos;Pires, Maria de Fátima ávila;Boddey, Robert Michael;
Pesquisa Agropecuária Brasileira , 2011, DOI: 10.1590/S0100-204X2011001000014
Abstract: the objective of this work was to evaluate the effect of legume trees and eucalyptus presence on litter dynamics of signal grass pasture (urochloa decumbens cv. basilisk). the litter dynamics was studied in a silvipastoril system and in monoculture for 12 months. the experimental design was in randomized blocks with six replicates and five animals per paddock. the existing plant litter and the deposited ones were evaluated. the mean existing litter was 5,038 and 3,944 kg ha-1 in the silvipastoril system and in monoculture, respectively. the silvipastoril system increased significantly the quantities of litter deposited. the quantity of nitrogen deposited in the pasture was greater in the silvipastoril system than in monoculture, 228 and 107 kg ha-1 per year, respectively. the presence of legumes and the eucalyptus in the silvipastoril system provided nitrogen, shown by 13c isotope analysis, and dry matter increases in the litter. increases in the production of litter rich in nitrogen can contribute for the sustainability of signal pastures in a long run.
Conflitos sociais contemporaneos: considera??es sobre o ambientalismo brasileiro
Ferreira, Lúcia da Costa;
Ambiente & Sociedade , 1999, DOI: 10.1590/S1414-753X1999000200004
Abstract: this article makes use of the analytical tools of the theory of action regarding social movements and contemporary social conflicts, as well as reflections about ngos, in order to outline a suitable explanatory model to understand relevant aspects of the contemporary environmentalism. the initial hypothesis is that the crisis experienced by leaders and militants is caused by a two-side difficulty: on one hand, its constitution as a unique social actor with a cultural project of society and, on the other hand, its double performance as a social actor and a political agent. from then on, our purpose is to understand the change caused by the social action of the environmental ngos in its everyday dimension. the ngos have an outstanding role in the deep process of social learning that mobilizes very different categories of social actors, as well as, in the final establishment of an environmental field. besides the national and international theoretical debate on the subject, this article is also based on the direct observation of the environmentalists everyday action since the cnumad-92.
Dimens?es humanas da biodiversidade: mudan?as sociais e conflitos em torno de áreas protegidas no Vale do Ribeira, SP, Brasil
Ferreira, Lúcia da Costa;
Ambiente & Sociedade , 2004, DOI: 10.1590/S1414-753X2004000100004
Abstract: this article aims to discuss the theoretical frames that supports biodiversity conservation policy in brazil: the paradigm of biodiversity isles surrounded by areas altered by predatory human action and the paradigm of regional biodiversity, which foresees the creation and maintenance of a network of protected areas integrated to their regional context and different perspectives of social dynamics within and in the surrounds of the areas taken as priorities for conservation. the main hypothesis is that the outcome of human activity in protected areas is mostly due to the social conflicts about these areas than to the aggregation of traditional populations into the process of natural resources sustainable management. the article will also analyze some results of previous research projects: forest and sea: utilization and conflicts in the ribeira valley, sp (fapesp n. 99/14514-1) and social conflicts and changes in the amazon and atlantic forest protected areas (fapesp n. 01/07992-1).
Uma Guerrilha Literária: Memória e cria o na obra N o Verás País Nenhum
Vera Lúcia da Silva
OPSIS : Revista do Departamento de História e Ciências Sociais , 2003,
Abstract: Este artigo pretende discutir a rela o entre o jornalismo e literatura presentes na obra N o Verás País Nenhum de Ignácio de Loyola Brand o
Dimens es do Programa Nacional de Humaniza o em Saúde no Brasil
Ana Lúcia da Silva
Saúde Coletiva , 2007,
Qualidade de vida na velhice e a pesquisa científica
Ana Lúcia da Silva
Saúde Coletiva , 2008,
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