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Search Results: 1 - 10 of 288463 matches for " Lígia Maria Suppo Souza;Margotto "
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Sobrevida e morbidade em prematuros com menos de 32 semanas de gesta??o na regi?o central do Brasil
Castro, Márcia Pimentel de;Rugolo, Lígia Maria Suppo Souza;Margotto, Paulo Roberto;
Revista Brasileira de Ginecologia e Obstetrícia , 2012, DOI: 10.1590/S0100-72032012000500008
Abstract: purpose: to evaluate the survival and complications associated with prematurity of infants with less than 32 weeks of gestation. methods: it was done a prospective cohort study. all preterm infants with a gestational age between 25 and 31 weeks and 6 days, born alive without congenital anomalies and admitted to the nicu between august 1st, 2009 and october 31st, 2010 were included. newborns were stratified into three groups: g25, 25 to 27 weeks and 6 days; g28, 28 to 29 weeks and 6 days; g30, 30 to 31 weeks and 6 days, and they were followed up to 28 days. survival at 28 days and complications associated with prematurity were evaluated. data were analyzed statistically by c2 test, analysis of variance, kruskal-wallis test, odds ratio with confidence interval (ci) and multiple logistic regression, with significance set at 5%. results: the cohort comprised 198 preterm infants (g25=59, g28=43 and g30=96). the risk of death was significantly higher in g25 and g28 compared to g30 (rr=4.14, 95%ci 2.23-7.68 and rr=2.84, 95%ci: 1.41-5.74). survival was 52.5%, 67.4% and 88.5%, respectively. survival was greater than 50% in preterm >26 weeks and birth weight >700 g. neonatal morbidity was inversely proportional to gestational age, except for necrotizing enterocolitis and leukomalacia, which did not differ among groups. logistic regression showed that pulmonary hemorrhage (or=3.3, 95%ci 1.4-7.9) and respiratory distress syndrome (or=2.5, 95%ci 1.1-6.1) were independent risk factors for death. there was a predominance of severe hemorrhagic brain lesions in g25. conclusion: survival above 50% occurred in infants with a gestational age of more than 26 weeks and >700 g birth weight. pulmonary hemorrhage and respiratory distress syndrome were independent predictors of neonatal death. it is necessary to identify the best practices to improve the survival of extreme preterm infants.
Fluorescent in situ hybridization (FISH) as a diagnostic tool for Williams-Beuren syndrome
Souza, Deise Helena de;Moretti-Ferreira, Danilo;Rugolo, Lígia Maria Suppo de Souza;
Genetics and Molecular Biology , 2007, DOI: 10.1590/S1415-47572007000100005
Abstract: fluorescent in situ hybridization (fish) with commercial probes covering the elastin gene (eln) was used to determine the frequency of the 7q11.23 deletion in 18 children clinically diagnosed with williams-beuren syndrome (wbs). a de novo deletion was detected in 15 of the children (83%). diagnostic investigation for wbs started late in childhood (median = 5.8 years). all the children showed facial features typical of the syndrome, mental retardation and developmental delay. over-friendliness was observed in the majority of cases. clinodactyly of the 5th finger (n = 13), cardiovascular disease (n = 9), loquacity (n = 9), low birthweight (n = 8), and failure to thrive (n = 9) were observed only in those children with the deletion. respiratory problems (n = 9), though not previously reported in the literature, was a common finding in the group studied. our results confirmed that fish is useful in identifying 7q11.23 deletions in cases of wbs. clinical manifestations were more evident in the deletion-positive children.
Detection of Oxacillin Resistance in Staphylococcus aureus Isolated from the Neonatal and Pediatric Units of a Brazilian Teaching Hospital
Valéria Cataneli Pereira, André Martins, Lígia Maria Suppo de Souza Rugolo and Maria de Lourdes Ribeiro de Souza da Cunha
Clinical Medicine Insights: Pediatrics , 2012,
Abstract: Objective: To determine, by phenotypic and genotypic methods, oxacillin susceptibility in Staphylococcus aureus strains isolated from pediatric and neonatal intensive care unit patients seen at the University Hospital of the Botucatu School of Medicine. Methods: A total of 100 S. aureus strains isolated from the following materials were studied: 25 blood cultures, 21 secretions, 12 catheters, 3 cannulae and one chest drain from 62 patients in the neonatal unit, and 36 blood cultures, one pleural fluid sample and one peritoneal fluid sample from 38 patients in the pediatric unit. Resistance of the S. aureus isolates to oxacillin was evaluated by the disk diffusion method with oxacillin (1 μg) and cefoxitin (30 μg), agar screening test using Mueller-Hinton agar supplemented with 6 μg/ml oxacillin and 4% NaCl, and detection of the mecA gene by PCR. In addition, the isolates were tested for β-lactamase production using disks impregnated with Nitrocefin and hyperproduction of β-lactamase using amoxicillin (20 μg) and clavulanic acid (10 μg) disks. Results: Among the 100 S. aureus strains included in the study, 18.0% were resistant to oxacillin, with 16.1% MRSA being detected in the neonatal unit and 21.0% in the pediatric unit. The oxacillin (1 μg) and cefoxitin (30 μg) disk diffusion methods presented 94.4% and 100% sensitivity, respectively, and 98.8% specificity. The screening test showed 100% sensitivity and 98.8% specificity. All isolates produced β-lactamase and one of these strains was considered to be a hyperproducer. Conclusions: The 30 μg cefoxitin disk diffusion method presented the best result when compared to the 1 μg oxacillin disk. The sensitivity of the agar screening test was similar to that of the cefoxitin disk diffusion method and higher than that of the oxacillin disk diffusion method. We observed variations in the percentage of oxacillin-resistant isolates during the study period, with a decline over the last years which might be related to improved nosocomial infection control and the rational use of antibiotics.
Detection of Oxacillin Resistance in Staphylococcus aureus Isolated from the Neonatal and Pediatric Units of a Brazilian Teaching Hospital
Valéria Cataneli Pereira,André Martins,Lígia Maria Suppo de Souza Rugolo,Maria de Lourdes Ribeiro de Souza da Cunha1
Clinical Medicine : Pediatrics , 2009,
Abstract: Objective: To determine, by phenotypic and genotypic methods, oxacillin susceptibility in Staphylococcus aureus strains isolated from pediatric and neonatal intensive care unit patients seen at the University Hospital of the Botucatu School of Medicine.Methods: A total of 100 S. aureus strains isolated from the following materials were studied: 25 blood cultures, 21 secretions, 12 catheters, 3 cannulae and one chest drain from 62 patients in the neonatal unit, and 36 blood cultures, one pleural fluid sample and one peritoneal fluid sample from 38 patients in the pediatric unit. Resistance of the S. aureus isolates to oxacillin was evaluated by the disk diffusion method with oxacillin (1 μg) and cefoxitin (30 μg), agar screening test using Mueller-Hinton agar supplemented with 6 μg/ml oxacillin and 4% NaCl, and detection of the mecA gene by PCR. In addition, the isolates were tested for β-lactamase production using disks impregnated with Nitrocefin and hyperproduction of β-lactamase using amoxicillin (20 μg) and clavulanic acid (10 μg) disks.Results: Among the 100 S. aureus strains included in the study, 18.0% were resistant to oxacillin, with 16.1% MRSA being detected in the neonatal unit and 21.0% in the pediatric unit. The oxacillin (1 μg) and cefoxitin (30 μg) disk diffusion methods presented 94.4% and 100% sensitivity, respectively, and 98.8% specificity. The screening test showed 100% sensitivity and 98.8% specificity. All isolates produced β-lactamase and one of these strains was considered to be a hyperproducer.Conclusions: The 30 μg cefoxitin disk diffusion method presented the best result when compared to the 1 μg oxacillin disk. The sensitivity of the agar screening test was similar to that of the cefoxitin disk diffusion method and higher than that of the oxacillin disk diffusion method. We observed variations in the percentage of oxacillin-resistant isolates during the study period, with a decline over the last years which might be related to improved nosocomial infection control and the rational use of antibiotics.
Hiperglicemia materna diária diagnosticada pelo perfil glicêmico: um problema de saúde pública materno e perinatal
Rudge, Marilza Vieira Cunha;Calderon, Iracema de Mattos Paranhos;Ramos, Maria Delgi;Brasil, Maria Aparecida Mour?o;Rugolo, Lígia Maria Suppo Souza;Bossolan, Grasiela;Odland, Jon ?yvind;
Revista Brasileira de Ginecologia e Obstetrícia , 2005, DOI: 10.1590/S0100-72032005001100010
Abstract: this is both a synthesis and a review of the major research findings, with the aim of validating rudge's group ib. in this group of pregnants, screening for gestational diabetes was positive while the diagnosis was negative (normal 100 g-oral glucose tolerance test 100 g-ogtt). nonetheless, the variations in glucose levels observed throughout the day, and confirmed by the glycemic profile (gp), characterized diurnal hyperglycemia, which accounts for maternal risk and adverse perinatal outcome. the description of this group is unique for both the establishment of the diagnosis during gestation and the follow-up of both the mother and the infant. these pregnancies have been erroneously classified as "low risk" and have not been diagnosed or treated. the ib group corresponds to 13.8% of the pregnant women screened in our service. this rate, added to the 7% of pregnancies complicated by diabetes, increase the occurrence of hyperglycemic disorders during gestation to up to 20.0%. in rudge's group ib: a) perinatal mortality rate is 41?, which is similar to that observed among diabetic pregnant women and 10 times higher than that found among non-diabetics; b) the observed placental abnormalities (both morphological and functional) differed from those seen in non-diabetic and diabetic pregnant women, indicating an adjustment to maintain functional activities that facilitated the passage of glucose to the fetus and explained fetal macrosomia (53.8% in non-treated pregnancies); c) maternal risk for hypertension, obesity and hyperglycemia was high and seemed to reproduce a model of metabolic syndrome, favoring the potential risk for future diabetes; d) 10 years after the index-pregnancy, type 2 diabetes was confirmed in 16.7% of the women in group ib. the authors suggest the development of multicentric studies in order to identify biomarkers specific for rudge's group ib and establish protocols for the diagnosis of gestational hyperglycemic disorders using the combination gp +
Human respiratory syncytial virus detection in children admitted at a community hospital in Botucatu, SP, Brazil
Bosso, Patrícia Alves Ramos;Candeias, Jo?o Manuel Grisi;Paduan, Karina dos Santos;Ricchetti, Sandra Mara Queiroz;Miranda, Antero Frederico Macedo de;Rugolo, Lígia Maria Suppo de Souza;Durigon, Edison Luiz;Ventura, Armando Morais;
Brazilian Journal of Microbiology , 2004, DOI: 10.1590/S1517-83822004000300015
Abstract: human respiratory syncytial virus (hrsv) is recognized as the single most important pathogen responsible for acute viral infection of the lower respiratory tract in young children. in this study, 84 samples from children under two years of age presenting acute respiratory symptoms were obtained from september 2000 to november 2001. analysis by immunofluorescence and reverse-transcription followed by pcr, revealed that 18% (15/84) of all the samples were positive for hrsv, in which 80% (12/15) of the cases was observed in children under six months of age and the subgroups a and b co-circulated in the studied period. these are the first data obtained for botucatu city and seasonality is evident by the higher circulation of the virus between may and july.
Fatores perinatais associados ao óbito precoce em prematuros nascidos nos centros da Rede Brasileira de Pesquisas Neonatais
Almeida, Maria Fernanda Branco de;Guinsburg, Ruth;Martinez, Francisco Eulógio;Procianoy, Renato S.;Leone, Cléa Rodrigues;Marba, Sérgio Tadeu Martins;Rugolo, Lígia Maria Sousa Suppo;Luz, Jorge Hecker;Lopes, José Maria de Andrade;
Jornal de Pediatria , 2008, DOI: 10.1590/S0021-75572008000400004
Abstract: objective:to evaluate perinatal factors associated with early neonatal death in preterm infants with birth weights (bw) of 400-1,500 g. methods: a multicenter prospective cohort study of all infants with bw of 400-1,500 g and 23-33 weeks of gestational age (ga), without malformations, who were born alive at eight public university tertiary hospitals in brazil between june of 2004 and may of 2005. infants who died within their first 6 days of life were compared with those who did not regarding maternal and neonatal characteristics and morbidity during the first 72 hours of life. variables associated with the early deaths were identified by stepwise logistic regression. results: a total of 579 live births met the inclusion criteria. early deaths occurred in 92 (16%) cases, varying between centers from 5 to 31%, and these differences persisted after controlling for newborn illness severity and mortality risk score (snappe-ii). according to the multivariate analysis, the following factors were associated with early intrahospital neonatal deaths: gestational age of 23-27 weeks (odds ratio - or = 5.0; 95%ci 2.7-9.4), absence of maternal hypertension (or = 1.9; 95%ci 1.0-3.7), 5th minute apgar 0-6 (or = 2.8; 95%ci 1.4-5.4), presence of respiratory distress syndrome (or = 3.1; 95%ci 1.4-6.6), and network center of birth. conclusion: important perinatal factors that are associated with early neonatal deaths in very low birth weight preterm infants can be modified by interventions such as improving fetal vitality at birth and reducing the incidence and severity of respiratory distress syndrome. the heterogeneity of early neonatal rates across the different centers studied indicates that best clinical practices should be identified and disseminated throughout the country.
Valor e varia??es da freqüência fundamental no choro de dor de recém-nascidos
Branco, Anete;Fekete, Saskia Maria W.;Rugolo, Lígia Maria S. de Souza;Rehder, Maria Inês;
Revista CEFAC , 2006, DOI: 10.1590/S1516-18462006000400014
Abstract: purpose: to study the value of fundamental frequency and its variations found in newborn pain cry. methods: emissions of 111 healthy and term newborns were recorded, whose lifetime varied from 24 to 72 hours, during venepuncture procedures. the acoustic analysis was realized with voxmetria 1.1 software, with an extract from the fundamental frequency value and gram 5.7, in order to verify the occurrence of fundamental frequency variations such as breaks, double harmonic breaks and higher fundamental frequency. the nips scale was carried out during venepuncture. the statistical analysis is descriptive with extract from the mean values, standard deviation and frequency of event occurrence. results: the newborns showed 100% of their emissions with frequency variations, in other words, breaks and double harmonic breaks. the higher fundamental frequency was found in 34.2% of the newborns. conclusion: the newborn communicates pain through cry. the newborn pain emission is tense and strident with higher fundamental frequency and variations founds at the spectrographic tracing such as breaks, double harmonic breaks and higher fundamental frequency. such characteristics are important to attract adult attention concerning as for a fast support to the newborns and to help in the evaluation of pain during a procedure.
Crescimento e desenvolvimento a longo prazo do prematuro extremo
Rugolo, Ligia Maria Suppo de Souza;
Jornal de Pediatria , 2005, DOI: 10.1590/S0021-75572005000200013
Abstract: objetive: to provide information for pediatricians and neonatologists to create realistic outcome expectations and thus help plan their actions. data sources: searches were made of the cochrane library, medline, and lilacs databases. summary of findings: the assessment of growth and development over the first 2-3 years must adjust chronological age with respect of the degree of prematurity. there is special concern regarding the prognoses of small for gestational age preterm infants, and for those with bronchopulmonary dysplasia. attention must be directed towards improving the nutrition of extremely low birth weight infants during their first years of life; these infants have high prevalence levels of failure to catch-up on growth, diseases and rehospitalizations during their first 2 years. they are frequently underweight and shorter than expected during early childhood, but delayed catch-up growth may occur between 8 and 14 years. extremely low birth weight infants are at increased risk of neurological abnormalities and developmental delays during their first years of life. educational, psychological, and behavioral problems are frequent during school years. teenage and adult outcomes show that although some performance differences persist, social integration is not impaired. conclusions: the growth and neurodevelopment of all elbw infants must be carefully monitored after discharge, to ensure that children and their families receive adequate support and intervention to optimize prognoses.
Cinética do escurecimeno n o-enzimático com solu es modelo de a úcares e aminoácidos em pH neutro e ácido = Kinetic of non-enzimatic browning with model solutions of sugar and aminoacids in neutral and acid pH
Vandré Barbosa Bri?o,Lígia Follmer,Marília de Souza,Vera Maria Rodrigues
Acta Scientiarum : Technology , 2011,
Abstract: A cor dos alimentos é um importante atributo para a escolha do consumidor. O escurecimento é desejável em alguns alimentos pela cor e pelo aroma produzidos (como nos casos do p o e carne assada), mas, é indesejável em outros (como no tratamento térmico de leite). A rea o de Maillard é influenciada pela natureza dos a úcares e aminoácidos envolvidos, bem como pelo pH e temperatura do processo. O objetivo do trabalho foi avaliar o escurecimento de solu es modelo contendo a úcares e aminoácidos pela rea o de Maillard em pH neutro e ácido, obtendo as taxas de rea o e avaliando a cinética dela. Doistipos de a úcares (glicose e lactose) foram misturados com dois tipos de aminoácidos (glicina ou glutamato de sódio) em pH 7,0 e pH 5,1. As solu es (2 mol L-1) foram aquecidas em água fervente (97oC), e a absorbancia medida (420 nm) em intervalos de tempo. A glicose apresentou maior taxa de rea o que a lactose, enquanto que entre os aminoácidos a glicina reagiu com intensidade semelhante ao glutamato. A redu o do pH do meio retarda a rea o, e o escurecimento demonstrou menor taxa de rea o em pH ácido. The color of food is an important attribute for consumer choice. Browning is desirable in some foods due to the color and flavor itproduces (such as in bread and roasted meat), but is undesirable for others (such as heattreated milk). The Maillard reaction is influenced by the nature of the sugars and amino acids involved, as well as the pH and temperature of the process. The aim of this work was to evaluate the browning of model solutions containing sugars and amino acids due to the Maillard reaction in neutral and acid pH, and to calculate the reaction rate and the kinetics of the reaction. Two types of sugars (glucose or lactose) were mixed with amino acids(glycine or sodium glutamate) in pH 7.0 or pH 5.1. The solutions (2 mol L-1) were heated in boiling water (97oC), and the absorbance was measured (420 nm) at time intervals. The glucose showed a higher reaction rate than lactose, whilst glycine reacted with the sameintensity as glutamate. The reduction in pH of the medium delays the reaction, and browning showed a slower reaction rate in acid pH.
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