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Search Results: 1 - 10 of 634678 matches for " Léa Assed Bezerra da;Tanomaru "
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Histological study of the effect of some irrigating solutions on bacterial endotoxin in dogs
Silva, Léa Assed Bezerra da;Leonardo, Mario Roberto;Assed, Sada;Tanomaru Filho, Mário;
Brazilian Dental Journal , 2004, DOI: 10.1590/S0103-64402004000200005
Abstract: the aim of this study was to evaluate, histopathologically, the effectiveness of mechanical preparation of root canals using different irrigating solutions in dog teeth filled with lps after pulpectomy. a total of 120 root canals of 6 mongrel dogs were filled with a solution of lps after pulpectomy. the irrigating solutions used were saline, 1, 2.5, and 5% sodium hypochlorite, and 2% chlorhexidine. no irrigation was used in the control group. the animals were sacrificed after 60 days and the teeth were fixed and demineralized. subsequently, serial 6-μm sections were stained with hematoxylin and eosin and mallory's trichrome for histopathological analysis and brown-brenn for verification of bacterial contamination. analysis showed that the inflammatory infiltrate was statistically less intense in the groups in which the root canals were irrigated with 5% sodium hypochlorite and 2% chlorhexidine. however, none of the irrigating solutions completely inactivated the harmful effects of lps. mechanical preparation associated with different irrigating solutions did not completely inactivate lps.
Effect of biomechanical preparation and calcium hydroxide pastes on the antisepsis of root canal systems in dogs
Soares, Janir Alves;Leonardo, Mario Roberto;Silva, Léa Assed Bezerra da;Tanomaru Filho, Mário;Ito, Izabel Yoko;
Journal of Applied Oral Science , 2005, DOI: 10.1590/S1678-77572005000100019
Abstract: in the endodontic treatment of root canals with necrotic pulps associated with periapical radiolucent areas, one of the main objectives of treatment consists in eliminating the microorganisms spread throughout the ramifications of the root canal system. the scope of this study was to evaluate the antiseptic efficacy of biomechanical preparation and two calcium hydroxide-based pastes, in dogs' teeth with experimentally induced chronic periapical lesions. after initial microbiological sampling, instrumentation of the root canals was undertaken using the conventional technique, using k type files used in conjunction with a solution of 5.25% sodium hypochlorite. after ninety-six hours, further microbiological sampling was undertaken and calen/cpmc or calasept pastes were applied for 15 and 30 days. ninety six hours after the removal of the medication, the third microbiological sampling was undertaken and finally histomicrobiological analysis followed using brown & brenn staining. the results were analyzed using the kruskall-wallis test, with a level of significance established at 5% (p<0.05). it was shown that the biomechanical preparation significantly reduced the number of colony forming units of microorganisms in root canals. in contrast, there was no significant difference between the antiseptic action of long-term dressings over two periods of action, although 30 days produced a greater reduction in the quantity of microorganisms and of positive microbiological cultures. in histological sections of the four experimental groups, similar microbiological patterns were shown (p>0.05), characterized by an elevated incidence of cocci, bacilli and filaments, predominantly gram-positive, in root canals, secondary canals and accessories, apical cementoplasts and dentinal tubules, but with a low incidence of microorganisms in areas of cementum resorption and the periapical lesion. the biomechanical preparation and intracanal dressing based on calcium hydroxide were important
Histomicrobiologic aspects of the root canal system and periapical lesions in dogs' teeth after rotary instrumentation and intracanal dressing with Ca(OH)2 pastes
Soares, Janir Alves;Leonardo, Mário Roberto;Silva, Léa Assed Bezerra da;Tanomaru Filho, Mário;Ito, Izabel Yoko;
Journal of Applied Oral Science , 2006, DOI: 10.1590/S1678-77572006000500011
Abstract: objective: the purpose of this study was to evaluate the distribution of microorganisms in the root canal system (rcs) and periapical lesions of dogs' teeth after rotary instrumentation and placement of different calcium hydroxide [ca(oh)2]-based intracanal dressings. materials and methods: chronic periapical lesions were experimentally induced in 80 premolar roots of four dogs. instrumentation was undertaken using the profile rotary system and irrigation with 5.25% sodium hypochlorite. the following ca(oh)2-based pastes were applied for 21 days: group 1 - calen (n=18); group 2 - calen+cpmc (n=20); group 3 - ca(oh)2 p.a. + anaesthetic solution (n=16) and group 4 - ca(oh)2 p.a.+ 2% chlorhexidine digluconate (n=18). eight root canals without endodontic treatment constituted the control group. histological sections were obtained and stained with brown & brenn staining technique to evaluate the presence of microorganisms in the main root canal, ramifications of the apical delta and secondary canals, apical cementoplasts, dentinal tubules, areas of cemental resorption and periapical lesions. the results were analyzed statistically by the mann-whitney u test (p<0.05). results: the control group showed the highest prevalence of microorganisms in all sites evaluated. gram-positive cocci, bacilli and filaments were the most frequent morphotypes. similar microbial distribution patterns in the rcs and areas of cementum resorption were observed in all groups (p>0.05). the percentage of rcs sites containing microorganisms in groups 1, 2, 3, 4 and control were: 67.6%, 62.5%, 78.2%, 62.0% and 87.6%, respectively. conclusion: in conclusion, the histomicrobiological analysis showed that the rotary instrumentation and the different calcium hydroxide pastes employed did not effectively eliminate the infection from the rcs and periapical lesions. however, several bacteria seen in the histological sections were probably dead or were inactivated by the biomechanical preparation and calci
Elimination of intracanal infection in dogs' teeth with induced periapical lesions after rotary instrumentation: influence of different calcium hydroxide pastes
Soares, Janir Alves;Leonardo, Mário Roberto;Silva, Léa Assed Bezerra da;Tanomaru Filho, Mário;Ito, Izabel Yoko;
Journal of Applied Oral Science , 2006, DOI: 10.1590/S1678-77572006000300005
Abstract: the aim of this study was to evaluate the antiseptic efficacy of rotary instrumentation associated with calcium hydroxide-based pastes prepared with different vehicles and antiseptics. chronic periapical lesions were experimentally induced in 72 premolar root canals of four dogs. under controlled asepsis, after initial microbiological sampling (a1), the root canals were instrumented using the profile system in conjunction with 5.25% sodium hypochlorite and the intracanal medication was placed. four experimental groups were formed according to the pastes used: group 1- calen (n=18), group 2- calen+cpmc (n=20), group 3- ca(oh)2 p.a.+ anaesthetic solution (n=16) and group 4- ca(oh)2 p.a.+ 2% chlorhexidine digluconate (n=18). after 21 days, the pastes were removed; the canals were emptied and 96 hours later a second microbiological sample was obtained (a2). the incidence of positive microbiological cultures and the number of cfus in stages a1 and a2 were compared statistically by the wilcoxon test while the influence of the different treatments in intracanal infection was evaluated by kruskal-wallis test at 5% significance level (p<0.05). large numbers of strict and facultative anaerobes, and viridans group streptococci were found in 100% of root canals of a1 samples. among a2 samples, all treatments showed significant reduction of cfus and positive cultures (p<0.05), but only groups 3 and 4 showed 100% of root canals free of microorganisms. rotary instrumentation plus naocl 5.25% associated with intracanal medication produced a drastic reduction or elimination of intracanal microbiota, whose performance was not influenced by the nature of the vehicle or the antiseptic added to the ca(oh)2 p.a.
Effect of different rotary instrumentation techniques and thermoplastic filling on apical sealing
Leonardo, Mario Roberto;Cervi, Devanir de Araújo;Tanomaru, Juliane Maria Guerreiro;Silva, Léa Assed Bezerra da;
Journal of Applied Oral Science , 2004, DOI: 10.1590/S1678-77572004000100016
Abstract: the aim of this study was to evaluate apical sealing after root canal treatment using two different rotary instrumentation techniques and two thermoplastic root canal filling techniques. the study was performed in 115 human extracted mandibular premolars. after coronary access the apical foramen was opened with a # 15 k file 1 mm beyond the apex. cleaning and shaping was subsequently carried out at the working length, 1 mm from the apex, with profile .04/.06 system (dentsply/maillefer), quantec (analytic endodontics/kerr) or by the step-back technique with 1% sodium hypochlorite solution as irrigating solution. the root canals were filled with thermafil (dentsply/maillefer) or microseal (analytic endodontics/kerr) or by lateral condensation technique using ah plus sealer (epoxy type). the teeth were immersed in 2% methylene blue under vacuum. then, they were longitudinally sectioned. the results showed that the association of profile and thermafil plus provide the best results (p<0.05). in conclusion, the association of different rotary instrumentation techniques and different filling systems influenced the apical sealing.
Residual antibacterial activity of chlorhexidine digluconate and camphorated p-monochlorophenol in calcium hydroxide-based root canal dressings
Soares, Janir Alves;Leonardo, Mario Roberto;Tanomaru Filho, Mário;Silva, Léa Assed Bezerra da;Ito, Izabel Yoko;
Brazilian Dental Journal , 2007, DOI: 10.1590/S0103-64402007000100003
Abstract: the purpose of this study was to evaluate the residual antibacterial activity of several calcium hydroxide [ca(oh)2]-based pastes, placed in root canals of dogs' teeth with induced chronic periapical lesions. root canals were instrumented with the profile rotary system and filled with 4 pastes: g1 (n=16): ca(oh)2 paste + anesthetic solution; g2 (n=20): calen? paste + camphorated p-monochlorophenol (cmcp); g3 (n=18): calen?; and g4 (n=18): ca(oh)2 paste + 2% chlorhexidine digluconate. after 21 days, the pastes were removed with size 60 k-files and placed on petri plates with agar inoculated with micrococcus luteus atcc 9341. pastes that were not placed into root canals served as control. after pre-diffusion, incubation and optimization, the inhibition zones of bacterial growth were measured and analyzed by mann-whitney u test at 5% significance level. all pastes showed residual antibacterial activity. the control samples had larger halos (p<0.05). the mean residual antibacterial activity halos in g1, g2, g3 and g4 were 7.6; 10.4; 17.7 and 21.4 mm, respectively. the zones of bacterial growth of g4 were significantly larger than those of g1 and g2 (p<0.05). in conclusion, regardless of the vehicle and antiseptic, all ca(oh)2-based pastes showed different degrees of measurable residual antibacterial activity. furthermore, unlike cmcp, chlorhexidine increased significantly the antibacterial activity of ca(oh)2.
Effect of rotary instrumentation and of the association of calcium hydroxide and chlorhexidine on the antisepsis of the root canal system in dogs
Soares, Janir Alves;Leonardo, Mario Roberto;Silva, Léa Assed Bezerra da;Tanomaru Filho, Mario;Ito, Izabel Yoko;
Brazilian Oral Research , 2006, DOI: 10.1590/S1806-83242006000200006
Abstract: this study aimed at evaluating the antisepsis of the root canal system (rcs) and periapical region (pr) provided by rotary instrumentation associated with chlorhexidine + calcium hydroxide as intracanal medicament. chronic periapical lesions were induced in 26 pre-molar roots in two dogs. after microbiological sampling, automatic instrumentation using the profile system and irrigation with 5.25% sodium hypochlorite solution, with a final rinse of 14.3% edta followed by profuse irrigation with physiological saline were carried out in 18 root canals. after drying the canals, a paste based on calcium hydroxide associated with a 2% chlorhexidine digluconate solution was placed inside them. after 21 days, the medication was removed, leaving the root canals empty and coronally sealed. after 96 hours, a final microbiological sample was obtained, followed by histomicrobiological processing by the brown & brenn method. eight untreated root canals represented the control group (c-g). based on the mann-whitney test at a confidence level of 5% (p < 0.05), the procedures of antisepsis used offered significant efficacy (p < 0.05) resulting in 100.0% of the canals free of microorganisms. in the c-g, an elevated incidence of various microbial morphotypes was confirmed in all sites of the rcs, with the presence of microbial colonies in the periapical region. in contrast, the experimental group showed a similar pattern of infection in the rcs, although less intense and a reduced level of periapical infection (p < 0.05). it was concluded that adequate instrumentation followed by the application of calcium hydroxide + chlorhexidine offered significant elimination of microorganisms.
Apical and periapical repair of dogs' teeth with periapical lesions after endodontic treatment with different root canal sealers
Leonardo, Mário Roberto;Salgado, Ant?nio Alberto Medeiros;Silva, Léa Assed Bezerra da;Tanomaru Filho, Mário;
Pesquisa Odontológica Brasileira , 2003, DOI: 10.1590/S1517-74912003000100013
Abstract: the aim of this study was to evaluate the apical and periapical repair after root canal treatment of dogs' teeth with pulp necrosis and chronic periapical lesion using different root canal sealers. after periapical lesion induction, forty-four root canals of 3 dogs were submitted to biomechanical preparation using 5.25% sodium hypochlorite as an irrigating solution. a calcium hydroxide dressing (calen pmcc) was applied for 15 days and the root canals were filled using the lateral condensation technique with gutta-percha points and sealapex, ah plus or sealer plus for sealing. after 180 days, the animals were sacrificed by anesthetic overdose and the obtained histological sections were stained with hematoxylin-eosin for optical microscopic analysis of the apical and periapical repair. the groups filled with sealapex and ah plus had better histological repair (p < 0.05) than the group filled with sealer plus, that had unsatisfactory results.
Apical and periapical repair of dogs' teeth with periapical lesions after endodontic treatment with different root canal sealers
Leonardo Mário Roberto,Salgado Ant?nio Alberto Medeiros,Silva Léa Assed Bezerra da,Tanomaru Filho Mário
Pesquisa Odontológica Brasileira , 2003,
Abstract: The aim of this study was to evaluate the apical and periapical repair after root canal treatment of dogs' teeth with pulp necrosis and chronic periapical lesion using different root canal sealers. After periapical lesion induction, forty-four root canals of 3 dogs were submitted to biomechanical preparation using 5.25% sodium hypochlorite as an irrigating solution. A calcium hydroxide dressing (Calen PMCC) was applied for 15 days and the root canals were filled using the lateral condensation technique with gutta-percha points and Sealapex, AH Plus or Sealer Plus for sealing. After 180 days, the animals were sacrificed by anesthetic overdose and the obtained histological sections were stained with hematoxylin-eosin for optical microscopic analysis of the apical and periapical repair. The groups filled with Sealapex and AH Plus had better histological repair (p < 0.05) than the group filled with Sealer Plus, that had unsatisfactory results.
In vivo microbiological evaluation of the effect of biomechanical preparation of root canals using different irrigating solutions
Tanomaru Filho, Mário;Yamashita, José Carlos;Leonardo, Mario Roberto;Silva, Léa Assed Bezerra da;Tanomaru, Juliane Maria Guerreiro;Ito, Izabel Yoko;
Journal of Applied Oral Science , 2006, DOI: 10.1590/S1678-77572006000200008
Abstract: the aim of this study was to evaluate the antimicrobial effect of biomechanical preparation using different irrigating solutions. seventy-eight root canals from premolars of four dogs were used. after experimental induction of periapical lesions, the root canals were prepared using the following solutions for irrigation: group 1) 2.5% sodium hypochlorite (naocl); group 2) 2% chlorhexidine (chx); group 3) saline solution and group 4) control group with no biomechanical preparation. the microbiological evaluation of the root canals was performed by counting the colony forming units (cfus) using different culture mediums. two absorbent paper cones were used in each root canal in order to collect the microbiological samples before, and thirty days after the biomechanical preparation. the culture plates were incubated in aerobic, anaerobic and microaerophilic environment. statistical evaluation was carried out using analysis of variance, tukey and student tests. the results demonstrated that there was reduction in the number of microorganisms in the naocl and chx groups (p<0.05). there was greater effectiveness in the chlorhexidine group. the group that used saline solution and the control group presented an increased number of microorganisms. it can be concluded that the use of antimicrobial irrigating solutions during biomechanical preparation promotes the reduction of endodontic microbiota. however, a considerable number of microorganisms were still observed.
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