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Search Results: 1 - 10 of 595111 matches for " László G Puskás "
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Transgenic fat-1 mouse as a model to study the pathophysiology of cardiovascular, neurological and psychiatric disorders
Undurti N Das, László G Puskás
Lipids in Health and Disease , 2009, DOI: 10.1186/1476-511x-8-61
Abstract: One traditional approach to modify tissue nutrient composition to study the effects of different diets is by supplementing the experimental groups with different diets consisting of many variations. Although this is an accepted mode of studying the effect of various nutrients and their effects on various physiological processes and pathologic situations, it is difficult to make all the dietary components identical, except the total energy, between two diets and perhaps the single component in which one is interested. The inevitable differences between diets and their components, however small they may be, may confound the study and contribute to inconsistencies or conflicting results observed. This is especially so for studies when one wishes to know the specific actions and functions of ω-3 and ω-6 fatty acids. In general, the effects of ω-3 and ω-6 fatty acids are studied supplementing the experimental groups of animals with different ω-3/ω-6 fatty acid ratios to establish the different fatty acid profiles in the tissues and extrapolate the results so obtained to the observed differences in the indices examined. In these studies, generally, fish oils and plant seed/vegetable oils are used to provide the required ω-3/ω-6 fatty acids respectively. Since these fatty acids are derived from different sources and are likely to contain other bioactive compounds, however minor they might be, are likely to affect the study outcomes. Furthermore, polyunsaturated fatty acids (PUFAs) are highly unstable and susceptible to oxidation. These variables arising from the diet and feeding procedures invariably affect the results.In view of these issues, it is ideal to develop a transgenic mouse capable of converting ω-6 to ω-3 fatty acids so that the results obtained in such an animal model will be more reliable and easy to interpret in terms of the effects of ω-3 and ω-6 fatty acids. Mice engineered to carry fat-1 gene from Caenorhabditis elegans can add a double bond into an unsat
MicroRNA profile of polyunsaturated fatty acid treated glioma cells reveal apoptosis-specific expression changes
Nóra Faragó, Liliána Z Fehér, Klára Kitajka, Undurti N Das, László G Puskás
Lipids in Health and Disease , 2011, DOI: 10.1186/1476-511x-10-173
Abstract: We evaluated the cytotoxic action of GLA, AA and DHA on glioma cells with specific reference to the expression of miRNAs. Relative expression of miRNAs were assessed by using high throughput nanocapillary real-time PCR. Most of the miRNA target genes that showed altered expression could be classified as apoptotic genes and were up-regulated by PUFA or temozolomide treatment, while similar treatments resulted in repression of the corresponding mRNAs, such as cox2, irs1, irs2, ccnd1, itgb3, bcl2, sirt1, tp53inp1 and k-ras.Our results highlight involvement of miRNAs in the induction of apoptosis in glioma cells by fatty acids and temozolomide.Malignant gliomas are among the most devastating of cancers and are a major cause of mortality in a young population with a median survival time of 9 months following cytoreductive surgery, radiotherapy and chemotherapy. Despite advances in surgery, chemotherapy, and radiotherapy, the prognosis of patients with this fatal disease has not improved significantly over the past 20 years [1,2]. Our previous studies showed that certain polyunsaturated fatty acids, especially γ-linolenic acid (GLA), arachidonic acid (AA), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) have tumoricidal action against glioma cells both in vitro and in vivo [3-7]. Understanding how the signaling pathways involved in surviving and inducing cell death of different glioma cells are regulated during PUFA treatment is important for the development of more effective tumor therapies including PUFAs alone or in combination with other drugs [8]. Several research groups have analyzed the mRNA expression profile of different cancer types to reveal novel gene markers for diagnosis and theraphy and to better understand the regulatory pathways and genetic networks [9,10].The mechanisms of action that are involved in the effects of PUFAs on cell regulation are complex. Thus, the use of microarrays, which allows the detection of differentially regulated mRNAs o
Light-trap Catch of the Common Cockchafer (Melolontha melolontha L.) Depending on the Atmospheric Ozone Concentration
Acta Silvatica & Lignaria Hungarica , 2011,
Abstract: The study deals the efficiency of light trapping of the Common Cockchafer (Melolonthamelolontha L.) (Coleoptera: Melolonthidae) in connection with the ozone concentration of air. Thedata of the Hungarian forestry light trap network were used for the years 1997 through 2006. Wecalculated relative catch values of from the number of caught insects. We assigned these to the ozonevalues of the respective days. For the classified date pairs regression equations were calculated. Weestablished that the light trapping is most effective if the ozone concentration is high. As opposed tothis, low ozone concentration reduces the success of the catch. Our results may be utilized in plantprotection and forest protection prognoses.
Pre- and Postfrontal Influences on Light Trapping of Winter Moth (Operophtera brumata L.)
Acta Silvatica & Lignaria Hungarica , 2008,
Abstract: Light trap catches of the winter moth (Operophtera brumata L.) getting from the data ofnational light-trap network were analysed in connection with weather fronts. It is concluded, thatweather fronts modify the catches of light traps according to their specific characters (main types) andaccording to their successions. It is worthy of attention, however, that even in those cases in which thefront reduces the number of insects caught, pre- and post-frontal influences often manifest themselvesin an increase of the number of collected specimens. Because of the frequency of weather fronts itwould be useful to take their effects into consideration on the quantity of insects captured at lighttraps.It is necessary to improve the applied method for this purpose.
Light-Trap Catch of the Harmful Moths Depending of Moonlight in North Carolina and Nebraska States of USA
László Nowinszky,János Puskás
ISRN Zoology , 2012, DOI: 10.5402/2012/238591
Abstract: The present study discusses the light trapping of harmful insects depending on the moonlight, its polarized percentage, and the moon phases. The trapping data were taken from light traps of North Carolina and Nebraska States of USA. We examined five species. The maximum individual number of species was collected at new moon or near the first quarter and last quarter. The farmers can use our results to forecast their plant protecting works. 1. Introduction The light trap is the most commonly used sampling device to study the daily activity of nocturnal insects. However, the effectiveness of light trapping as an insect sampling method was influenced by many environmental factors. Great many studies in professional literature are devoted to the role of the moon in modifying light-trapping catch. The conclusions are contradictory and up to this day good many questions have remained unqualified. Some authors report an inability to detect a clear decrease in the efficiency of the light trap as a consequence of the effect of moonlight. Williams [1] recognized that at the time of a full moon far fewer insects caught the light traps than at the time of a new moon. According to Williams [1], Williams et al. [2], and El-Ziady [3], the reasons for a smaller catch at a full moon might be as follows.(a) Moonlight reduces the activity of insects and so the active population accessible for the light trap is smaller.(b) The light of the lamp collects moths from a smaller area in a moonlit environment. In recent decades no scientist could give a provable answer to this question; in fact, most have not even tried. Based on latter concept, several researchers calculated collection distances for different light-trap types under the variation of lunar cycle (Dufay [4], Bowden [5], and Nowinszky [6]). Bowden and Morris [7] made some corrections for daily catches by an index calculated from the changes of collecting distance during lunation. The following authors explain the lower catches recorded when there is a full moon in terms of a shorter collecting distance: Vaishampayan and Shrivastava [8], Vaishampayan and Verma [9], Nag and Nath [10] also suggest that the lower catches of light traps when there is a Full Moon may be due to the stronger and brighter light of the Moon and a smaller collecting area, which are clearly physical phenomena. Gy?rfi [11] attributes the much smaller numbers of insects caught by light traps at full moon to decreased activity. Nemec [12] is of the view that moths are inactive at full moon. Based on the results of their studies, Baker and Sadovy
Metabolic syndrome influences cardiac gene expression pattern at the transcript level in male ZDF rats
Márta Sárk?zy, ágnes Zvara, Nóra Gyémánt, Veronika Fekete, Gabriella F Kocsis, Judit Pipis, Gerg? Sz?cs, Csaba Csonka, László G Puskás, Péter Ferdinandy, Tamás Csont
Cardiovascular Diabetology , 2013, DOI: 10.1186/1475-2840-12-16
Abstract: Fasting blood glucose, serum insulin, cholesterol and triglyceride levels were measured at 6, 16, and 25 wk of age in male ZDF and lean control rats. Oral glucose tolerance test was performed at 16 and 25 wk of age. At week 25, total RNA was isolated from the myocardium and assayed by rat oligonucleotide microarray for 14921 genes. Expression of selected genes was confirmed by qRT-PCR.Fasting blood glucose, serum insulin, cholesterol and triglyceride levels were significantly increased, glucose tolerance and insulin sensitivity were impaired in ZDF rats compared to leans. In hearts of ZDF rats, 36 genes showed significant up-regulation and 49 genes showed down-regulation as compared to lean controls. Genes with significantly altered expression in the heart due to metabolic syndrome includes functional clusters of metabolism (e.g. 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2; argininosuccinate synthetase; 2-amino-3-ketobutyrate-coenzyme A ligase), structural proteins (e.g. myosin IXA; aggrecan1), signal transduction (e.g. activating transcription factor 3; phospholipase A2; insulin responsive sequence DNA binding protein-1) stress response (e.g. heat shock 70kD protein 1A; heat shock protein 60; glutathione S-transferase Yc2 subunit), ion channels and receptors (e.g. ATPase, (Na+)/K+ transporting, beta 4 polypeptide; ATPase, H+/K+ transporting, nongastric, alpha polypeptide). Moreover some other genes with no definite functional clusters were also changed such as e.g. S100 calcium binding protein A3; ubiquitin carboxy-terminal hydrolase L1; interleukin 18. Gene ontology analysis revealed several significantly enriched functional inter-relationships between genes influenced by metabolic syndrome.Metabolic syndrome significantly alters cardiac gene expression profile which may be involved in development of cardiac pathologies in the presence of metabolic syndrome.
Edaravone Protects against Methylglyoxal-Induced Barrier Damage in Human Brain Endothelial Cells
Andrea E. Tóth, Fruzsina R. Walter, Alexandra Bocsik, Petra Sántha, Szilvia Veszelka, Lajos Nagy, László G. Puskás, Pierre-Olivier Couraud, Fuyuko Takata, Shinya Dohgu, Yasufumi Kataoka, Mária A. Deli
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0100152
Abstract: Background Elevated level of reactive carbonyl species, such as methylglyoxal, triggers carbonyl stress and activates a series of inflammatory responses leading to accelerated vascular damage. Edaravone is the active substance of a Japanese medicine, which aids neurological recovery following acute brain ischemia and subsequent cerebral infarction. Our aim was to test whether edaravone can exert a protective effect on the barrier properties of human brain endothelial cells (hCMEC/D3 cell line) treated with methylglyoxal. Methodology Cell viability was monitored in real-time by impedance-based cell electronic sensing. The barrier function of the monolayer was characterized by measurement of resistance and flux of permeability markers, and visualized by immunohistochemistry for claudin-5 and β-catenin. Cell morphology was also examined by holographic phase imaging. Principal Findings Methylglyoxal exerted a time- and dose-dependent toxicity on cultured human brain endothelial cells: a concentration of 600 μM resulted in about 50% toxicity, significantly reduced the integrity and increased the permeability of the barrier. The cell morphology also changed dramatically: the area of cells decreased, their optical height significantly increased. Edaravone (3 mM) provided a complete protection against the toxic effect of methylglyoxal. Co-administration of edaravone restored cell viability, barrier integrity and functions of brain endothelial cells. Similar protection was obtained by the well-known antiglycating molecule, aminoguanidine, our reference compound. Conclusion These results indicate for the first time that edaravone is protective in carbonyl stress induced barrier damage. Our data may contribute to the development of compounds to treat brain endothelial dysfunction in carbonyl stress related diseases.
Combination of Small Molecule Microarray and Confocal Microscopy Techniques for Live Cell Staining Fluorescent Dye Discovery
Eszter Molnár,Soujanya Kuntam,Pradeep Kumar Reddy Cingaram,Begüm Peksel,Bhavyashree Suresh,Gabriella Fábián,Liliána Z. Fehér,Attila Bokros,ágnes Medgyesi,Ferhan Ayaydin,László G. Puskás
Molecules , 2013, DOI: 10.3390/molecules18089999
Abstract: Discovering new fluorochromes is significantly advanced by high-throughput screening (HTS) methods. In the present study a combination of small molecule microarray (SMM) prescreening and confocal laser scanning microscopy (CLSM) was developed in order to discover novel cell staining fluorescent dyes. Compounds with high native fluorescence were selected from a 14,585-member library and further tested on living cells under the microscope. Eleven compartment-specific, cell-permeable (or plasma membrane-targeted) fluorochromes were identified. Their cytotoxicity was tested and found that between 1–10 micromolar range, they were non-toxic even during long-term incubations.
Polyunsaturated fatty acids synergize with lipid droplet binding thalidomide analogs to induce oxidative stress in cancer cells
László G Puskás, Liliána Z Fehér, Csaba Vizler, Ferhan Ayaydin, Erzsébet Rásó, Eszter Molnár, István Magyary, Iván Kanizsai, Márió Gyuris, Ramóna Madácsi, Gabriella Fábián, Klaudia Farkas, Péter Hegyi, Ferenc Baska, Béla ózsvári, Klára Kitajka
Lipids in Health and Disease , 2010, DOI: 10.1186/1476-511x-9-56
Abstract: Cytotoxicity was detected in different cell lines including melanoma, leukemia, hepatocellular carcinoma, glioblastoma at micromolar concentrations. The synthesized analogs are non-toxic to adult animals up to 1 g/kg but are teratogenic to zebrafish embryos at micromolar concentrations with defects in the developing muscle. Treatment of tumor cells resulted in calcium release from the endoplasmic reticulum (ER), induction of reactive oxygen species (ROS), ER stress and cell death. Antioxidants could partially, while an intracellular calcium chelator almost completely diminish ROS production. Exogenous docosahexaenoic acid or eicosapentaenoic acid induced calcium release and ROS generation, and synergized with the analogs in vitro, while oleic acid had no such an effect. Gene expression analysis confirmed the induction of ER stress-mediated apoptosis pathway components, such as GADD153, ATF3, Luman/CREB3 and the ER-associated degradation-related HERPUD1 genes. Tumor suppressors, P53, LATS2 and ING3 were also up-regulated in various cell lines after drug treatment. Amino-phthalimides down-regulated the expression of CCL2, which is implicated in tumor metastasis and angiogenesis.Because of the anticancer, anti-angiogenic action and the wide range of applicability of the immunomodulatory drugs, including thalidomide analogs, lipid droplet-binding members of this family could represent a new class of agents by affecting ER-membrane integrity and perturbations of ER homeostasis.Cytoplasmic lipid-droplets (LDs) are common inclusions of eukaryotic cells. Little is known about the composition or physiological role of LDs, however growing number of evidences imply that LDs are not solely static inclusions for storage of excess lipid, but they are dynamic and functionally active [1-4]. Although LD biogenesis is not well understood, it is assumed that the LDs form within the two leaflets of the ER membrane to function as lipid storage sites [5]. LDs are active inclusions with e
Rt-PCR Analysis and Evolutionary Relationship of Some Hungarian Grapevine leafroll associated virus 1 and 3 Isolates  [PDF]
Eszter Cseh, András Péter Takács, Richard Gáborjányi, László Palkovics, László Kocsis
American Journal of Plant Sciences (AJPS) , 2013, DOI: 10.4236/ajps.2013.410250

Hungarian isolates of Grapevine leafroll associated virus 1 and 3 (GLRaV-1, GLRaV-3) were tested using serological (DAS-ELISA) and molecular (RT-PCR) methods. Five hundred bp long PCR products of the part of HSP70 gene of one serologically positive GLRaV-1 and four GLRaV-3 isolates were sequenced. These sequences were applied for phylogenetic analysis and compared to foreign virus isolates of NCBI GenBank. Phylogenetic analysis of GLRaV-1 HSP70 gene supported the earlier results that it could be divided into two clusters: E and A. The Hungarian isolate 6.4.1 belonged to the group E. This isolate showed the highest homology with the AY754914 isolate from the Czech Republic. GLRaV-3 sequence data could cluster five groups. Hungarian 2.2; 3.5 and 4.2 isolates were estimated belonging to the group II. The 1.4 isolate from the same vineyard as 2.2 varied in sequence data so it belonged to the other, IV. variant group with two South African, two Austrian and a Syrah isolate. According to the phylogenetic analysis, two variant groups occurred in Hungary. These isolates related with each other, but showed higher similarity of foreign counties. In some cases, they were similar to isolates of the neighbour countries such as Slovakia and Austria. It could be supposed that mainly the exchange of virus infected propagation materials caused the dissemination of GLRaV isolates.

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