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Search Results: 1 - 10 of 40053 matches for " Kyung-Min Kim "
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Analysis of Genetic and Biogeography Diversity of Korean Indigenous Wild Rocambole (Allium monanthum Max)  [PDF]
Gihwan Yi, Kyung-Min Kim
American Journal of Plant Sciences (AJPS) , 2013, DOI: 10.4236/ajps.2013.45130

This study was conducted to obtain baseline data describing Allium monanthum Max. for breeding an elite cultivar. A. monanthum has recently become popular vegetable in Korea owing to its unique flavors and nutraceutical effects. The agronomic traits of 271 accessions of A. monanthum collected throughout Korea were evaluated in this study. Leaf width showed the greatest variation (0.1 to 25 mm) among traits evaluated, followed by leaf sheath length and number of cormlets. The average leaf sheath length and diameter, which are commonly used to evaluate quality in the market, were 1.29 cm and 0.3 cm. Some superior accessions had a leaf sheath length of >10 cm and diameter of >1.1 cm. The average weight of the cormlets ranged from 1 to 2 g; however, one accession weighed more than 4 g. The genetic diversity of A. monanthum calculated based on morphology and molecular distance was greater in Jeolla and Gyeoungsang provinces than Gyeonggi and Chungcheong provinces. Finally, seven elite accessions of A. monanthum were selected owing to their potential usefulness in future breeding projects.

Effect of Maltose Concentration on Plant Regeneration of Anther Culture with Different Genotypes in Rice (Oryza sativa L.)  [PDF]
Seul Gi Park, Mohammad Ubaidillah, Kyung-Min Kim
American Journal of Plant Sciences (AJPS) , 2013, DOI: 10.4236/ajps.2013.411279

This study describes the impact of different concentrations of maltose on plant regeneration of anther culture for five genotypes of rice (Oryza sativa). N6 medium was used for calli induction, while N6 medium supplemented with different concentrations of maltose, 2.0 mg/L NAA and 0.5 mg/L kinase was used for plant regeneration. The result showed that during the initial stages of calli induction the anther cultures had varying rates of calli formation among genotypes, with the best frequency being observed for Dreami2/CaMsrB2-8-DH-1 with a calli frequency of 27.8%. Different genotypes of rice cultured in regeneration media showed varying plantlet regeneration on media supplemented with different concentrations of maltose, with low concentrations (0.04 g/L) leading to low frequency regeneration plantlet but high green plant production. Indeed, when Dreami2/CaMsrB2-8-DH-2 and Dreami2/CaMsrB2-8-DH-5 were cultivated under these conditions, 100% green plants were observed. Another genotype also showed a small rate of albino frequency in response to the lowest concentration of maltose, while increased maltose concentrations

Effective Procedure for Development of EST-SSR Markers Using cDNA Library  [PDF]
Kyung A Kim, Hee-Cheon Park, Jae-Keun Sohn, Kyung-Min Kim
American Journal of Plant Sciences (AJPS) , 2012, DOI: 10.4236/ajps.2012.39159
Abstract: The present study was conducted to develop EST-SSR markers using the cDNA library from rice plant. Total RNA extracted from the leaves of brown plant hopper resistance gene originated from a rice cultivar “Cheongcheong” and sensitive rice cultivar “Nakdong” were used to synthesize a cDNA library. As a result of analyzing the cDNA library, the 17 EST-SSR primer sets were developed. This study enables to provide effective marker assisted selection (MAS) methods on the selection of white-backed planthopper resistance gene originated from a rice plant more simply, quickly and precisely. Furthermore, using this marker’s advantage of deriving from cDNA, it is possible to identify the white-backed planthopper resistance gene. In addition, this study introduces a technique for construction of a cDNA library safely without using radioactivity.
Analysis of Rice Grain Quality-Associated Quantitative Trait Loci by Using Genetic Mapping  [PDF]
Byung-Wook Yun, Min-Gyu Kim, Tri Handoyo, Kyung-Min Kim
American Journal of Plant Sciences (AJPS) , 2014, DOI: 10.4236/ajps.2014.59125

The main objective of this research was to identify quantitative trait loci associated with rice qualities to provide reliable information for marker-assisted selection and development of new varieties. In total, 120 doubled haploid (DH) lines developed by another culture from the F1 hybrid of a cross between “Cheongcheong”, a Tongil variety, and “Nagdong”, a japonica variety, were used. A microsatellite linkage map of 222 markers spanned 2082.4 centimorgans (cM) and covered 12 rice chromosomes with an average interval of 9.4cM between markers. Eight quantitative trait loci (QTLs) were associated with rice quality, consisting of two QTLs on chromosomes 1 and 9 for amylose content; three QTLs on chromosomes 8, 9, and 10 for protein content; and three QTLs on chromosomes 2, 3, and 6 for lipid content. PCR expression levels measured using the SSR markers RM23914 for proteins and RM6266 for lipids, and RM586 showed a higher degree of amplification. The present study should be useful for improving the nutritional quality of rice by means of marker-assisted selection.

Physicochemical Characteristics and QTL Mapping Associated with the Lipid Content of High-Lipid Rice  [PDF]
Na-Hye Kim, Jae-Keun Sohn, Kyung-Min Kim
American Journal of Plant Sciences (AJPS) , 2013, DOI: 10.4236/ajps.2013.410241

This study was conducted to examine the physicochemical characteristics and perform QTL mapping of genetic factors associated with the lipid content of rice. A rice strain with a high lipid content, P31-2-2-2-B-B, was developed from mutants of Dongjin created by T-DNA insertion. The lipid content of P31-2-2-2-B-B brown rice was 4.42% whereas that of the donor cultivar Dongjin was 2.56%. The total fatty acid content of the high-lipid mutant brown rice was 7.82% and that of Dongjin was 3.43%. The unsaturated fatty acid composition of the mutant brown rice was 2.73% oleic acid, 2.74% linoleic acid, and 0.34% linolenic acid. In contrast, the fatty acid composition of the donor cultivar Dongjinwas 1.30% oleic acid and 0.99% linoleic acid. The percentage of unsaturated fatty acid to total fatty acid in the high-lipid mutant was higher

QTL Analysis of Yield Components in Rice Using a Cheongcheong/Nagdong Doubled Haploid Genetic Map  [PDF]
Gyu Hwan Park, Jin-Hee Kim, Kyung-Min Kim
American Journal of Plant Sciences (AJPS) , 2014, DOI: 10.4236/ajps.2014.59130

In this study, only two of 12 quantitative trait loci (QTLs) affecting yield and yield components were identified in a single year, indicating that individual QTLs are probably sensitive to the environment. A rice growth survey of “Cheongcheong” and “Nag dong” in a doubled haploid population in 2012 revealed that yield capacity was influenced by climate change. Analysis of yield and yield components indicated that five average traits are high in “Cheongcheong”. Frequency distribution tables indicated that panicles per plant (PPP), spike lets per panicle (SPP), and 1000-grain weight (TGW) were normally distributed. The strongest relationship was identified between SPP and seed set percentage (SSP) among phenotypic correlations related to yield and yield components found on chromosomes 2, 3, 6, 8 in 2012. SPP and SSP was a very relevant requisite about quantity. Analysis of QTL about quantity was total 9. In the present study, a doubled haploid population was used to analyze the epistatic effects on yield and yield components in rice. Although other epistatic QTLs were not included in any of the main-effect QTLs, they significantly influenced the traits. These results indicated that epistatic interaction plays an important role in controlling the expression of complex traits. Thus, the utilization of marker-assisted selection in rice breeding programs should take epistatic effects into consideration. Hence, the QTLs responsible for major effects are more suitable for marker-assisted selection programs to improve yield and related traits across different environments.

Response of Explants of Calli Rice (Oryza sativa L.) Japonica cv. “Ilmi” to Gene Transformation Using Agrobacterium tumefaciens-Mediated  [PDF]
Fika Ayu Safitri, Mohammad Ubaidillah, Miswar  , Kyung-Min Kim
American Journal of Plant Sciences (AJPS) , 2013, DOI: 10.4236/ajps.2013.44102

The AtBI-1 gene encoding the Arabidopsis thaliana Bax inhibitor was introduced into Japonica cultivars of rice (Ilmi) by Agrobacterium-mediated transformation, and a large number of transgenic plants were produced. The neomycin phosphotransferase II (NPTII) gene was used as a selectable marker. The activity of neomycin phosphotransferase could be successfully detected in transgenic rice calluses. Introduction of the AtBI-1 gene was also confirmed by PCR using AtBI-1 specific oligonucleotide primers in regenerated plants. Stable integration and expression of the AtbI-1 gene in plants were confirmed by GFP analysis.

Functional Analysis for Rolling Leaf of Somaclonal Mutants in Rice (Oryza sativa L.)  [PDF]
Young-Hie Park, Hyun-Suk Lee, Gi-Hwan Yi, Jae-Keun Sohn, Kyung-Min Kim
American Journal of Plant Sciences (AJPS) , 2011, DOI: 10.4236/ajps.2011.21008
Abstract: This study was carried out to facilitate the functional analysis of rice genes. Some 297 insertion plants (1.7%) of the entire lines with the endogenous retrotransposon Tos17 were produced. Phenotypes of these plants in the S2 genera-tion were observed in the field according to different leaf types. Rolling leaf mutants showed thinner sclerenchyma-tous cells, defective arrangement of vascular bundles, and well-formed bulliform cells as compared to the parental cultivar. Two new copies of Tos17 were detected in the rolling leaf type. In the new leaf type, the copy number and activation of Tos12, 15 did not appear as ‘Ilpum’. Flanking sequence tag (FST) analysis of Tos17 in the rolling leaf mutant indicated that new copies of Tos17 were transposed on chromosomes 11 and 12. Annotated homologues of the tagging genes on chromosome 11 were arabinoxylan rabinofuranohydrolase isoenzyme AXAH-I and II. The tagging gene in chromosome 12 was highly correlated with 6 kinds of genes including a transcript regulated factor and a rough sheath 2-like protein. This rolling leaf and flanking sequence data will stimulate the functional analysis of rice genes.
Analysis of High-Resolution QTL Markers Associated with Rice Yields Using Data for Two Consecutive Years in Different Environmental Conditions  [PDF]
Ye-Jin Son, Gyu-Ho Lee, Hyun-Suk Lee, Tri Handoyo, Byung-Wook Yun, Kyung-Min Kim
Natural Science (NS) , 2014, DOI: 10.4236/ns.2014.611080

Previously we reported the identification of seven quantitative trait loci (QTLs) associated with the rice yield measuring five parameters including panicles per plant (PPP), spikelets per panicle (SPP), seed set percentage (SSP), 1000-grain weight (TGW) and yield in 2012. Here we report the analysis of QTLs using the same trait parameters data of the mapping population in 2013 for detecting highly conserved QTL markers. A total of 6 QTLs were identified from chromosomes 1, 7, 8, 10, 11, and 12, which were contrasted with our previous results (chromosomes 1, 2, 4, 5, 6, 8, and 11). In this comparison, three QTLs from chromosome 1, 8, and 11 were only found to be associated with the components of yield over two consecutive years indicating high sensitivity of QTL markers to the environment. Of those three QTLs, SPP-associated marker RM12285 was found to be dominantly expressed by real-time PCR (qPCR). In addition, compared to our previous report the numbers of mapping population and markers were significantly increased for higher resolution markers from 70 to 120, and from 143 to 217, respectively. We also found that the parameter SPP was dominantly correlated with the rice yield. Furthermore, the double haploid (DH) population facilitated to analyze the epistatic effects for yield and yield components in rice. Taken together, combining multiple mapping population data over years possibly enables narrowing down to the highly conserved QTL markers against diverse environmental fluctuation caused by such as drought and high temperature. Thus, these data would be critically exploited to improve for the crop breeding strategy.

First-principles simulation of the optical response of bulk and thin-film α-quartz irradiated with an ultrashort intense laser pulse
Kyung-Min Lee,Chul Min Kim,Shunsuke A. Sato,Tomohito Otobe,Yasushi Shinohara,Kazuhiro Yabana,Tae Moon Jeong
Physics , 2013, DOI: 10.1063/1.4864662
Abstract: A computational method based on a first-principles multiscale simulation has been used for calculating the optical response and the ablation threshold of an optical material irradiated with an ultrashort intense laser pulse. The method employs Maxwell's equations to describe laser pulse propagation and time-dependent density functional theory to describe the generation of conduction band electrons in an optical medium. Optical properties, such as reflectance and absorption, were investigated for laser intensities in the range $10^{10} \, \mathrm{W/cm^{2}}$ to $2 \times 10^{15} \, \mathrm{W/cm^{2}}$ based on the theory of generation and spatial distribution of the conduction band electrons. The method was applied to investigate the changes in the optical reflectance of $\alpha$-quartz bulk, half-wavelength thin-film and quarter-wavelength thin-film and to estimate their ablation thresholds. Despite the adiabatic local density approximation used in calculating the exchange--correlation potential, the reflectance and the ablation threshold obtained from our method agree well with the previous theoretical and experimental results. The method can be applied to estimate the ablation thresholds for optical materials in general. The ablation threshold data can be used to design ultra-broadband high-damage-threshold coating structures.
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