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Search Results: 1 - 10 of 7702 matches for " Kuo-Yen Hung "
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Current Evidence of the Monoaminergic Regulation of Romantic Love and Relationship  [PDF]
Wei-Hsi Chen, Mei-Yen Chu, Yuan-Hsiang Chu, Yen-Chin Lin, Kuo-Yen Chen
Advances in Sexual Medicine (ASM) , 2017, DOI: 10.4236/asm.2017.73011
Abstract:
Introduction: Sexual response cycle is modulated by a variety of biological factors, especially antidepressants and antipsychotics. Although the circuit of romantic love has recently been delineated, the biological impact on romantic love is barely mentioned in human, however. Methods: In thus study, a comprehensive literature review was completed to elucidate the role of central monoamines, including dopamine, serotonin, norepinephrine, epinephrine, melatonin and histamine, for the romantic love and relationship in human. Results: The central circuit of romantic love includes the mesolimbic and mesocortical tract and their extensions widely distributed in cortical, subcortical and brainstem structures. The activation of brain foci progressively decreases along with the romantic relationship in many areas, including the bilateral caudate and ventral tegmentum area without change of passionate love. Hyperactivity of dopamine and serotonin is suggested at the synaptic level for romantic love in lovers, probably through an inhibition of transporters or increase of terminal release. Romantic love can be modulated by antidepressants or recreational drugs. Polymorphism of dopamine and serotonin receptor relates to the romantic relationship. The effect of other monoamines is barely mentioned. Conclusions: Romantic love is vulnerable for secondary effect, such as drugs which modulate the dopamine and serotonin, in human. Romantic relationship is also impacted by the polymorphism of monoamine receptors. Therefore, the biological effect is not limited to sexual response cycle but also the romantic love and relationship. Further investigation is warranted for understanding the secondary effect in romantic love for romantic dissolution or divorce.
A highly efficient β-glucosidase from the buffalo rumen fungus Neocallimastix patriciarum W5
Hsin-Liang Chen, Yo-Chia Chen, Mei-Yeh J Lu, Jui-Jen Chang, Hiaow-Ting C Wang, Tzi-Yuan Wang, Sz-Kai Ruan, Tao-Yuan Wang, Kuo-Yen Hung, Hsing-Yi Cho, Huei-Mien Ke, Wan-Ting Lin, Ming-Che Shih, Wen-Hsiung Li
Biotechnology for Biofuels , 2012, DOI: 10.1186/1754-6834-5-24
Abstract: In this study, a cDNA encoding β-glucosidase was isolated from the buffalo rumen fungus Neocallimastix patriciarum W5 and is named NpaBGS. It has a length of 2,331 bp with an open reading frame coding for a protein of 776 amino acid residues, corresponding to a theoretical molecular mass of 85.1 kDa and isoelectric point of 4.4. Two GH3 catalytic domains were found at the N and C terminals of NpaBGS by sequence analysis. The cDNA was expressed in Pichia pastoris and after protein purification, the enzyme displayed a specific activity of 34.5 U/mg against cellobiose as the substrate. Enzymatic assays showed that NpaBGS was active on short cello-oligosaccharides from various substrates. A weak activity in carboxymethyl cellulose (CMC) digestion indicated that the enzyme might also have the function of an endoglucanase. The optimal activity was detected at 40°C and pH 5?~?6, showing that the enzyme prefers a weak acid condition. Moreover, its activity could be enhanced at 50°C by adding Mg2+ or Mn2+ ions. Interestingly, in simultaneous saccharification and fermentation (SSF) experiments using Saccharomyces cerevisiae BY4741 or Kluyveromyces marxianus KY3 as the fermentation yeast, NpaBGS showed advantages in cell growth, glucose production, and ethanol production over the commercial enzyme Novo 188. Moreover, we showed that the KY3 strain engineered with the NpaNGS gene can utilize 2 % dry napiergrass as the sole carbon source to produce 3.32 mg/ml ethanol when Celluclast 1.5 L was added to the SSF system.Our characterizations of the novel β-glucosidase NpaBGS revealed that it has a preference of weak acidity for optimal yeast fermentation and an optimal temperature of ~40°C. Since NpaBGS performs better than Novo 188 under the living conditions of fermentation yeasts, it has the potential to be a suitable enzyme for SSF.
Functional characterization of cellulases identified from the cow rumen fungus Neocallimastix patriciarum W5 by transcriptomic and secretomic analyses
Tzi-Yuan Wang, Hsin-Liang Chen, Mei-Yeh J Lu, Yo-Chia Chen, Huang-Mo Sung, Chi-Tang Mao, Hsing-Yi Cho, Huei-Mien Ke, Teh-Yang Hwa, Sz-Kai Ruan, Kuo-Yen Hung, Chih-Kuan Chen, Jeng-Yi Li, Yueh-Chin Wu, Yu-Hsiang Chen, Shao-Pei Chou, Ya-Wen Tsai, Te-Chin Chu, Chun-Chieh A Shih, Wen-Hsiung Li, Ming-Che Shih
Biotechnology for Biofuels , 2011, DOI: 10.1186/1754-6834-4-24
Abstract: We have developed an efficient platform that uses a combination of transcriptomic and proteomic approaches to N. patriciarum to accelerate gene identification, enzyme classification and application in rice straw degradation. By conducting complementary studies of transcriptome (Roche 454 GS and Illumina GA IIx) and secretome (ESI-Trap LC-MS/MS), we identified 219 putative GH contigs and classified them into 25 GH families. The secretome analysis identified four major enzymes involved in rice straw degradation: β-glucosidase, endo-1,4-β-xylanase, xylanase B and Cel48A exoglucanase. From the sequences of assembled contigs, we cloned 19 putative cellulase genes, including the GH1, GH3, GH5, GH6, GH9, GH18, GH43 and GH48 gene families, which were highly expressed in N. patriciarum cultures grown on different feedstocks.These GH genes were expressed in Pichia pastoris and/or Saccharomyces cerevisiae for functional characterization. At least five novel cellulases displayed cellulytic activity for glucose production. One β-glucosidase (W5-16143) and one exocellulase (W5-CAT26) showed strong activities and could potentially be developed into commercial enzymes.Cellulosic ethanol produced by microbial fermentation from feedstocks has been proposed to replace fossil fuels in transportation. A key step in cellulosic ethanol production is to break down cellulose into glucose and hemicellulose into xylose, which can subsequently be converted into ethanol by fermentative microbes. Therefore, finding efficient cellulases is important to bioethanol production, as well as for hydrolyzing feedstocks into sugars in general. Neocallimastix species is one of the major anaerobic fungi in the rumen of water buffalo capable of efficiently digesting cellulosic biomass [1-4]. Such anaerobic fungi are potential sources for highly active cellulolytic enzymes that are useful for cellulose hydrolysis [5-7]. Plant cell wall degrading enzymes from rumen fungi such as Neocallimastix patriciarum may
Synthesis of Amino Core Compounds of Galactosyl Phytosyl Ceramide Analogs for Developing iNKT-Cell Inducers
Yin-Cheng Huang,Li-Wu Chiang,Kai-Shiang Chang,Wen-Chin Su,Yi-Hsian Lin,Kee-Ching Jeng,Kun-I Lin,Kuo-Yen Liao,Ho-Lein Huang,Chung-Shan Yu
Molecules , 2012, DOI: 10.3390/molecules17033058
Abstract: 1-Aminophytosphingosine and 6-aminogalactosyl phytosphingosine were prepared in 61% and 40% yield libraries with 44 carboxylic acids showed that a 4-butylbenzoic acid-derived product exe, respectively. Glycosylation using benzoyl-protected lipid resulted in better a-selectivity for ceramide analogs, but the yield was less than that obtained with benzyl moieties. Screening the amide rted less cytotoxicity. These analogs were purified for validation of immunological potencies and the a-GalCer analog but not the sphingosine analog stimulated human iNKT cell population.
Multifocal Demyelinating Leukoencephalopathy Induced by Levamisole Therapy
Hsiu-Ping Chang,Hung-Chou Kuo,Tzu-Chen Yen,Chin-Chang Huang
Chang Gung Medical Journal , 2006,
Abstract: The neurotoxic effects of levamisole therapy have been rarely reported. In this article,we report two patients who developed multifocal demyelinating leukoencephalopathy(MDL) after levamisole treatment. Clinically, they had dizziness, nausea, vomiting, confusion,mental dullness, speechlessness, and memory impairment after the treatment. The totaldosage of levamisole was 300 mg and 1800 mg, respectively. The onset of symptoms wasacute or subacute, from a few hours to several weeks. Initial brain magnetic resonance imaging(MRI) demonstrated diffuse multifocal enhancing lesions in the subcortical white matter.After the withdrawal of levamisole, and during the follow-up period, the neurological andneuropsychological manifestations and serial brain MRI improved gradually. We concludethat levamisole treatment may induce MDL, and that the brain MRI changes may correlatewell with the recovery of clinical features and neuropsychological findings. In addition,early steroid treatment is crucial, and the neurotoxic effect of levamisole is not dose-dependentand may be idiosyncratic or immune-mediated.
Fault-tolerant Operations for Universal Blind Quantum Computation
Chia-Hung Chien,Rodney Van Meter,Sy-Yen Kuo
Computer Science , 2013,
Abstract: Blind quantum computation is an appealing use of quantum information technology because it can conceal both the client's data and the algorithm itself from the server. However, problems need to be solved in the practical use of blind quantum computation and fault-tolerance is a major challenge. On an example circuit, the computational cost measured in T gates executed by the client is 97 times more than performing the original computation directly, without using the server, even before applying error correction. (The client still benefits due to drastically reduced memory requirements.) Broadbent et al. proposed running error correction over blind computation, but our first protocol applies one layer of Steane's [[7,1,3]] code underneath instead. This protocol has better fault tolerance, but still results in a substantial overhead. We propose another protocol to reduce the client's computational load by transferring the qubit preparation to the server. For each logical qubit used in the computation, the client is only required to receive eight logical qubits via teleportation then buffer two logical qubits before returning one. This protocol also protects the client's fault-tolerant preparation of logical qubits from a side-channel attack.
Invasion of Artificial Vascular Graft into Duodenal Bulb after Living Donor Liver Transplantation  [PDF]
Yu-Ju Hung, Kuo-Hua Lin, Chen-Te Chou, Chih-Jan Ko, Ping-Yi Lin, Chia-En Hsieh, Hsu-Heng Yen, Yao-Li Chen
Surgical Science (SS) , 2015, DOI: 10.4236/ss.2015.62012
Abstract:

Artificial vessel grafts are used for outflow reconstruction in living donor liver transplantation (LDLT). We report a very rare case of invasion of an artificial vascular graft into the duodenal bulb after LDLT. A 54-year-old man underwent LDLT in August 2012. A right hemiliver without a middle hepatic vein was used as a graft. The outflow of segment V was created by anatomizing an artificial graft to the inferior vena cava. During outpatient follow-up, the patient complained of acid regurgitation but did not experience abdominal pain. Panendoscopy was arranged and it revealed that the artificial graft had invaded the duodenal bulb. Operation was performed to remove the graft and the patient discharged after fourteen days of hospital stay. Invasion of artificial vascular grafts to adjacent organs is rare, but happens in living donor liver transplantation. It may be caused by inappropriate vessel graft length and adhesion between the graft and adjacent organs.

Insulin resistance is associated with hepatocellular carcinoma in chronic hepatitis C infection
Chao-Hung Hung, Jing-Houng Wang, Tsung-Hui Hu, Chien-Hung Chen, Kuo-Chin Chang, Yi-Hao Yen, Yuan-Hung Kuo, Ming-Chao Tsai, Sheng-Nan Lu, Chuan-Mo Lee
World Journal of Gastroenterology , 2010,
Abstract: AIM: To elucidate the role of insulin resistance (IR) and serum adiponectin level in hepatocellular carcinoma (HCC) associated with chronic hepatitis C.METHODS: Clinical and biochemical characteristics were collected from 165 consecutive patients with newly diagnosed HCC. Homeostasis model assessment of IR (HOMA-IR) and serum adiponectin level were investigated in 188 patients with different stages of hepatitis C virus (HCV) infection.RESULTS: Among HCC patients, type 2 diabetics (DM) was more prevalent in HCV subjects (35.6%, n = 59) compared to hepatitis B virus (HBV; 12.7%, n = 63) or non-HBV, non-HCV cases (7.1%, n = 28). In patients with chronic hepatitis C, HCC subjects had higher blood sugar (P < 0.001), insulin level (P = 0.003) and HOMA-IR (P = 0.018) than those with chronic hepatitis and advanced fibrosis. Age, male sex and body mass index were significantly associated with serum adiponectin level, whereas HOMA-IR was not. Based on stepwise logistic regression analysis, age (OR: 1.124, P < 0.001), serum insulin level (OR: 1.585, P < 0.001), HOMA-IR (OR: 0.495, P = 0.001), DM (OR: 11.601, P = 0.002) and male sex (OR: 3.877, P = 0.016) were independently associated with HCC. This result was similar even if the diabetic subjects were excluded for analysis.CONCLUSION: Insulin resistance measured by HOMA-IR, regardless of the presence of diabetes, is significantly associated with HCC development in patients with chronic HCV infection.
The Clinical Role of CT-Based Morphologic Description in Severely Calcified Coronary Arteries Ectasia Encountering Acute Coronary Syndrome
Yen-Yu Liu,Jui-Peng Tsai,Chung-Lieh Hung,Jiun-Yi Li,Jen-Yuan Kuo
Case Reports in Cardiology , 2012, DOI: 10.1155/2012/267496
Abstract: Diffuse coronary arteries ectasia combined with calcification is seldom reported. Acute coronary syndrome, a potentially life-threatening disease, accompanied with coronary ectasia and diffuse calcification, made percutaneous coronary intervention difficult and risky owing to increasing complications rate. Dual-source computed tomography and three-dimensional volume rendering images help cardiovascular surgeon easier to localize the ideal site and facilitate the procedure.
The efficacy of four-slice helical CT in evaluating pancreatic trauma: a single institution experience
Wei-Jing Lee, Ning-Ping Foo, Hung-Jung Lin, Yen-Chang Huang, Kuo-Tai Chen
Journal of Trauma Management & Outcomes , 2011, DOI: 10.1186/1752-2897-5-1
Abstract: We undertook a retrospective review of all blunt trauma patients admitted to the Chi-Mei Medical Center from January 2004 to June 2006. Every patients underwent abdominal CT scan in emergency department and the CT scans were obtained with a four-slice helical CT. Diagnosis of a pancreatic injury in these patients was by surgical observation or by CT findings. Radiographic pancreatic injuries were classified as deep or superficial lesions. Deep lesions were defined as the hematomas or lacerations >50% thickness of the pancreas. Superficial lesions were described as the hematomas or lacerations <50% thickness of the pancreas; pancreatic edema; and focal fluid accumulation around the pancreasNineteen patients with pancreatic trauma, fourteen males and five females, average age 40.6 ± 21.4 years, were included. Most patients (73.7%) with pancreatic trauma had associated organ injuries. CT was performed in all patients and laparotomy in 14 patients. CT was 78.9% sensitive in detecting pancreatic trauma. All deep pancreatic lesions revealed on CT required surgical treatment, and complication was discovered in two patients undergoing delayed surgery. Superficial lesions were managed conservatively.Four-slice helical CT can detect most pancreatic trauma and provide practical therapeutic guidance. Delayed operation might result in complications and is associated with prolonged hospital stays.Pancreatic trauma is uncommon and most trauma surgeons have little experience in managing the condition [1,2]. To complicated matters, most patients with pancreatic trauma have concomitant injuries, [2] which frequently obscure the symptoms of pancreatic trauma and distract the attention of the trauma surgeon. Serum amylase and lipase tests have been proved neither sensitive nor specific [3,4]. Moreover, the pancreas is deeply seated in the retroperitoneum and there are difficulties using physical examination, sonography and diagnostic peritoneal lavage to investigate this area [1,5,6].T
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