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Search Results: 1 - 10 of 14254 matches for " Kunling Shen "
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Status of Mycoplasma pneumoniae Pneumonia in Chinese Children: A Systematic Review  [PDF]
Qiang Qin, Baoping Xu, Xiuyun Liu, Kunling Shen
Advances in Microbiology (AiM) , 2014, DOI: 10.4236/aim.2014.411076
Abstract: Mycoplasma pneumoniae pneumonia (MPP) becomes one of the most important health problems in China recently. Date for MPP inChinais scarce. Although macrolides and/or cortical steroids had been reported to be effective treatment for MPP, the long-term outcome remained uncertain. A study on status of MPP in China was conducted via a systematic review of published studies which have the Chinese data and collected from published PubMed and core journals of China Knowledge Resource Integrated Database (CNKI). The analysis was based on epidemiology, clinical characteristics, treatment, drug resistance and prognosis. Twenty five articles concerned about MPP in Chinese children and adolescent were enrolled, including 11 studies on epidemiology/etiology, 11 studies on clinical characteristics, 7 studies on drug resistance, 5 studies on treatment from mainland China respectively. The overall incidence of MPP ranged from 7.1% to 54.4%. Fever and cough were most frequently identified in manifestations. Drug resistance to macrolides ranged from 18.9% to 90%. The outcome of treatment in patients who received combined treatment of macrolides, cephalosporin antibiotics and/or cortical steroid seems to be better than those who received macrolides only. Macrolide combined with cephalosporin or cortical steroid both may decrease the severity of MPP in the past decade. There was not enough evidence to suggest that cortical steroid can decrease the mortality of MPP in children. And a multi-center, randomized double blind research on the effect of cortical steroid was encouraged.
Characterization of variants in the promoter of BZLF1 gene of EBV in nonmalignant EBV-associated diseases in Chinese children
Yingkang Jin, Zhengde Xie, Gen Lu, Shuang Yang, Kunling Shen
Virology Journal , 2010, DOI: 10.1186/1743-422x-7-92
Abstract: The objective of this study was to investigate the relationship between EBV genotypes (Zp variants and EBV type 1 and 2) and the clinical phenotypes of EBV-associated diseases in Chinese children. The Zp region was directly sequenced in 206 EBV-positive DNA samples from the blood of patients with IM, EBV-HLH, CAEBV, and healthy controls. Type 1 or type 2 EBV was examined by PCR for EBNA2 and EBNA3C subtypes. Four polymorphic Zp variants were identified: Zp-P, Zp-V3, Zp-P4 and Zp-V1, a new variant. The Zp-V3 variant was significantly associated with CAEBV (P ≤ 0.01). The frequency of co-infection with Zp variants was higher in patients with CAEBV and EBV-HLH, compared with IM and healthy controls, mostly as Zp-P+V3 co-infection. Type 1 EBV was predominant in all categories (81.3-95%) and there was no significant difference in the frequency of the EBV types 1 and 2 in different categories (P > 0.05).Type 1 EBV and BZLF1 Zp-P of EBV were the predominant genotypes in nonmalignant EBV associated diseases in Chinese children and Zp-V3 variant may correlates with the developing of severe EBV infection diseases, such as CAEBV and EBV-HLH.Epstein-Barr virus (EBV) is a member of the Lymphocryptovirus genus, Gammaherpesvirinae subfamily of the Herpesviridae family of viruses. This virus is associated with a wide variety of diseases, both benign and malignant, which ubiquitously infect humans and persist for the lifetime of the individual. During its life cycle, EBV has latent and productive (lytic) phases. The latent phase maintains the virus long-term in its host and can lead to the productive phase where virus is reactivated and produced allowing it to be transmitted. During the two phases, EBV expresses a set of viral gene products in its life cycle and some of these genes were proved to possess the potential to cause changes in the interactions between the virus and the host's immune system [1,2].The biology and pathogenesis of EBV has been the focus of many studies but th
The knowledge, attitudes and practices of parents of children with asthma in 29 cities of China: a multi-center study
Jing Zhao, Kunling Shen, Li Xiang, Guoqing Zhang, Meng Xie, Juan Bai, Qiyi Chen
BMC Pediatrics , 2013, DOI: 10.1186/1471-2431-13-20
Abstract: A KAP questionnaire was distributed to parents caring for 2960 children (0–14 years) diagnosed with asthma for at least 3 months from China’s 29 provinces. A 50-item questionnaire was devised for this cross-sectional survey based on a comprehensive review of the subject. Questionnaires were scored on 30 items regarding parent asthma-related KAP, with one point for every correct response and a possible range of 0–13 for knowledge, 0–7 for attitudes and 0–10 for practices. Higher scores indicated better KAP. Chi-squared tests and logistic regression were used to identify factors associated with practices and combined KAP scores.The response rate was 83.95% (2485/2960). Only 18.31% (455/2485) of parents correctly answered ≥ 60% of the knowledge questions (mean = 5.69). Most (89.85%; 2226/2485) gave positive responses to ≥ 60% of the attitude questions (mean = 5.23) while 67.89% (1687/2485) correctly answered ≥ 60% of the practices questions (mean = 6.19). Knowledge and attitudes were positively associated with pulmonary function testing, regular physician visits, monitoring with a peak flow meter and the Children’s Asthma Control Test questionnaire, avoidance of asthma triggers, using an inhaled β2 receptor agonist and adherence to medication regimen (p ≤ 0.05). Attitudes were also associated with allergen testing. In logistic regression analysis, high KAP scores (dichotomized by a cut-off score of 18) were positively associated with food allergy, rhinitis, physician visits, frequency of visits and parent education (p < 0.05, OR > 1).Generally, the parents’ KAP were poor. A gap between recommended and actual practice was observed, which may be related to inadequate knowledge about and poor attitudes toward childhood asthma. Improving knowledge and attitudes may encourage better practices among parents of children with asthma.Asthma is one of the common chronic diseases among children. Increases in asthma prevalence, morbidity and mortality have intensified public healt
Self-reported prevalence of childhood allergic diseases in three cities of China: a multicenter study
Jing Zhao, Juan Bai, Kunling Shen, Li Xiang, Sui Huang, Aihuan Chen, Ying Huang, Jiansheng Wang, Rongwei Ye
BMC Public Health , 2010, DOI: 10.1186/1471-2458-10-551
Abstract: We conducted a cross-sectional survey between October 2008 and May 2009 in three major cities of China (Beijing, Chongqing, and Guangzhou) to evaluate the prevalence rates of childhood allergic diseases including asthma, allergic rhinitis, and eczema, using a questionnaire of the International Study of Asthma and Allergies in Childhood (ISAAC) group. A total of 24,290 children aged 0-14 years were interviewed, using a multi-stage sampling method. To acquire data on children aged 3-14 years, we visited schools and kindergartens. To access children too young to attend school or kindergarten, we extended our survey to community health service centers. Each questionnaire was completed by a parent or guardian of a child after an informed consent form was signed.Of the 24,290 children in our study, 12,908 (53.14%) were males and 11,382 (46.86%) females; 10,372 (42.70%) were from Beijing, 9,846 (40.53%) from Chongqing, and 4,072 (16.77%) from Guangzhou. Our survey indicated that in Beijing, Chongqing, and Guangzhou, the prevalence rates of asthma were 3.15%, 7.45%, and 2.09%, respectively; the rates of allergic rhinitis were 14.46%, 20.42%, and 7.83%; and the rates of eczema were 20.64%, 10.02%, and 7.22%. The prevalence of allergic diseases varied with age. Asthma was relatively less common both in children aged under 2 years, and in those aged 9 years or more, in each of the three cities. The prevalence of allergic rhinitis was also lower in children younger than 2 years. The prevalence of eczema fell with age.A marked increase in the prevalence rates of allergic diseases in China (compared with earlier data) was evident. Further studies exploring the precise causes of this increase are warranted.Over 25% of the world's population suffer from allergic diseases including asthma, allergic rhinitis, eczema, and drug reactions [1]. Allergic diseases have become one of the top three conditions demanding a major effort toward prevention and control in the 21st century, accordi
Human Parainfluenza Virus-Associated Respiratory Tract Infection among Children and Genetic Analysis of HPIV-3 Strains in Beijing, China
Naiying Mao, Yixin Ji, Zhengde Xie, Huanhuan Wang, Huiling Wang, Junjing An, Xinxin Zhang, Yan Zhang, Zhen Zhu, Aili Cui, Songtao Xu, Kunling Shen, Chunyan Liu, Weizhong Yang, Wenbo Xu
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0043893
Abstract: The relevance of human parainfluenza viruses (HPIVs) to the epidemiology of acute respiratory infections (ARI) in China is unclear. From May 2008 to September 2010, 443 nasopharyngeal aspirates (NPAs) from hospitalized pediatric patients (age from 1 to 93 months) in Beijing were collected and screened for HPIVs and other common respiratory viruses by real-time RT-PCR. Sixty-two of 443 samples were positive for HPIVs with 4 positive for HPIV-2 and 58 positive for HPIV-3, indicating that HPIV-3 was the predominant virus present during the study period. A phylogenetic tree based on all the available HN (hemagglutinin-neuraminidase) sequences of HPIV-3 indicated that three distinct clusters (A,B, and C) were circulating with some temporal and regional clustering. Cluster C was further divided into sub-clusters, C1, C2, C3 and C4. HPIV-3 from Beijing isolates belonged to sub-cluster C3, and were grouped with the isolates from two Provinces of China and the neighboring country of Japan. Genetic analysis based on entire HN gene revealed that the HPIV-3 isolates from Beijing were highly similar with 97.2%–100% identity at the nucleotide level and these could be divided into two closely related lineages, C3a and C3b. These findings suggested that there was co-circulation of multiple lineages of HPIV-3 in the Beijing region during the study period. This is the first study to describe the epidemiology and molecular characterization of HPIVs in China.


系统科学与数学 , 1982,
Abstract: 设 X_1,X_2,…是独立同分布的 m 维随机变量,其共同的概率密度(对 Lebesgue 测度而言)为 f(x),x∈R~m.设 f∈C_(kα),C_(kα)表示 R~m 中一族概率密度,其所有 k 阶混合偏导数存在、连续且绝对值不超过α.设整数 r=r_1+…+r_m,r_1,…,r_m≥0.本文考虑 f 的 r 阶混合偏导数
ARIMA Model in the Application of Shanghai and Shenzhen Stock Index  [PDF]
Shichang Shen, Yue Shen
Applied Mathematics (AM) , 2016, DOI: 10.4236/am.2016.73016
Abstract: In the paper, based on the data of Shanghai and Shenzhen 300 stock index in 2011, the ARIMA model was established by using Eviews 6, and the historical trend of stock price was found out. The model was used to provide a reference for the investors.
Angiotensin II mediates the high-glucose-induced endothelial-to-mesenchymal transition in human aortic endothelial cells
Rining Tang, Qing Li, Linli Lv, Houyong Dai, Min Zheng, Kunling Ma, Bicheng Liu
Cardiovascular Diabetology , 2010, DOI: 10.1186/1475-2840-9-31
Abstract: Primary human aortic endothelial cells (HAECs) were divided into three groups: a normal glucose (NG) group, HG group, and irbesartan (1 μM)-treated (HG+irbesartan) group. The concentration of angiotensin II in the supernatant was detected by radioimmunoassay. Pathological changes were investigated using fluorescence microscopy and electron microscopy. Immunofluorescence staining was performed to detect the co-expression of CD31 and fibroblast markers, such as fibroblast-specific protein 1 (FSP1). The expressions of FSP1 and α-SMA were detected by RT-PCR and Western blot.The treatment of HAECs in the HG group resulted in significant increases in the expressions of FSP1 and angiotensin II in dose-and time-dependent manners. The incubation of HAECs exposure to HG resulted in a fibroblast-like phenotype, wherein increased microfilamentation and a roughened endoplasmic reticulum structure were observed in the cytoplasm. The expressions of FSP1 and α-SMA were significantly increased in the HG group, and these changes were inhibited by irbesartan treatment (P < 0.05). Double staining of the HAECs indicated a co-localization of CD31 and FSP1 and that some cells acquired spindle-shaped morphologies and a loss of CD31 staining; however, treatment with irbesartan attenuated the expression of EndMT (P < 0.05).These findings suggest a novel mechanism in HG-induced endothelial damage via the mediation of the EndMT by angiotensin II, which was inhibited by Irbesartan.Vascular complications, such as cardiomyopathy and nephropathy, are the leading cause of morbidity and mortality in patients with diabetes. Because the initial injury by hyperglycemia occurs in the blood vessels, endothelial cells are considered to be the first target, and, furthermore, endothelial damage plays an important role in the development and progression of diabetic vascular complications [1-3]. Four main molecular mechanisms have been implicated in glucose-mediated vascular disease: the glucose-induced activ
Bovicin HJ50-Like Lantibiotics, a Novel Subgroup of Lantibiotics Featured by an Indispensable Disulfide Bridge
Jian Wang, Hongchu Ma, Xiaoxuan Ge, Jie Zhang, Kunling Teng, Zhizeng Sun, Jin Zhong
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0097121
Abstract: Lantibiotics are ribosomally-synthesized and posttranslationally modified peptides with potent antimicrobial activities. Discovery of novel lantibiotics has been greatly accelerated with the soaring release of genomic information of microorganisms. As a unique class II lantibiotic, bovicin HJ50 is produced by Streptococcus bovis HJ50 and contains one rare disulfide bridge. By using its precursor BovA as a drive sequence, 16 BovA-like peptides were revealed in a wide variety of species. From them, three representative novel lan loci from Clostridium perfringens D str. JGS1721, Bacillus cereus As 1.348 and B. thuringiensis As 1.013 were identified by PCR screening. The corresponding mature lantibiotics designated perecin, cerecin and thuricin were obtained and structurally elucidated to be bovicin HJ50-like lantibiotics especially by containing a conserved disulfide bridge. The disulfide bridge was substantiated to be essential for the function of bovicin HJ50-like lantibiotics as its disruption eliminated their antimicrobial activities. Further analysis indicated that the disulfide bridge played a crucial role in maintaining the hydrophobicity of bovicin HJ50, which might facilitate it to exert antimicrobial function. This study unveiled a novel subgroup of disulfide-containing lantibiotics from bacteria of different niches and further demonstrated the indispensable role of disulfide bridge in these novel bovicin HJ50-like lantibiotics.
Understanding the Determinants of Consumers’ Willingness to Pay for Eco-Labeled Products: An Empirical Analysis of the China Environmental Label  [PDF]
Junyi Shen
Journal of Service Science and Management (JSSM) , 2012, DOI: 10.4236/jssm.2012.51011
Abstract: This study applies data from a web-based survey conducted in mainland China to examine the determinants of consumers’ willingness to pay (WTP) for seven different product categories awarded with China Environmental Label and com- pare the mean WTP estimates among these categories. The Interval Regression method is used for estimation. The results indicate that Chinese consumers who regard environmental conservation as being more important than life convenience, who believe purchasing the eco-labeled products is good for the environment, and who have the experience in purchasing eco-labeled products are willing to pay more for those products with environmental label or eco-label. In addition, socio-demographic characteristics such as gender, age, education and household income are found to be important factors to affect Chinese consumers’ WTP amounts. Finally, the results of pair-wise comparison among the mean WTP estimates of various eco-labeled products indicate that most of them are different, which implies that the degrees of Chinese consumers’ willingness to pay extra money for China Environmental Label are different based on the types of products.
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