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Search Results: 1 - 10 of 64 matches for " Konradin Metze "
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Changes of texture features due to image compression
Metze Konradin,Adam Randall L,Leite Neucimar J
Diagnostic Pathology , 2010, DOI: 10.1186/1746-1596-5-s1-s15
Abstract:
Computerized texture analysis of atypical immature myeloid precursors in patients with myelodysplastic syndromes: an entity between blasts and promyelocytes
Joyce R Vido, Randall L Adam, Irene GH Lorand-Metze, Konradin Metze
Diagnostic Pathology , 2011, DOI: 10.1186/1746-1596-6-93
Abstract: In May-Grünwald-Giemsa stained BM smears of 30 newly diagnosed MDS patients and 19 cases of normal BM, nuclei of blasts and promyelocytes were digitalized and interactively segmented. The morphological classification of the cells was done by consensus of two observers. Immature granulocytic precursors, which could not be clearly classified either as blasts or promyelocytes, were called "atypic myeloid precursors". Nuclear morphometry and texture features derived from the co-occurrence matrix and fractal dimension (FD) were calculated.In normal BM, when compared to myeloblasts, nuclei of promyelocytes showed significant increase in perimeter and local texture homogeneity and a decrease in form factor, chromatin gray levels, Haralick's entropy, inertia, energy, contrast, diagonal moment, cluster prominence, the fractal dimension according to Minkowski and its goodness-of-fit. Compared to normal myeloblast nuclei, the chromatin texture of MDS myeloblasts revealed higher local homogeneity and goodness-of-fit of the FD, but lower values of entropy, contrast, diagonal moment, and fractal dimension. The same differences were found between nuclei of normal promyelocytes and those of MDS. Nuclei of atypical myeloid precursors showed intermediate characteristics between those of blasts and promyelocytes according to the quantitative features (perimeter, form factor, gray level and its standard deviation), but were similar to promyelocytes according to the texture variables inertia, energy, contrast, diagonal moment, cluster prominence, and Minkowski's fractal dimension.BM atypical immature myeloid precursors are difficult to be correctly classified in routine cytology. Although their cytoplasm is more similar to that of myeloblasts, computerized texture analysis indicates a nuclear chromatin remodeling more close to the promyelocyte, thus indicating an asynchronous intermediate maturation stage between blast and promyelocyte.Myelodysplastic syndromes (MDS) are a group of hemo
Fractal dimension of chromatin is an independent prognostic factor for survival in melanoma
Valcinir Bedin, Randall L Adam, Bianca CS de Sá, Gilles Landman, Konradin Metze
BMC Cancer , 2010, DOI: 10.1186/1471-2407-10-260
Abstract: We examined 71 primary superficial spreading cutaneous melanoma specimens (thickness ≥ 1 mm) from patients with a minimum follow up of 5 years. Nuclear area, form factor and fractal dimension of chromatin texture were obtained from digitalized images of hematoxylin-eosin stained tissue micro array sections. Clark's level, tumor thickness and mitotic rate were also determined.The median follow-up was 104 months. Tumor thickness, Clark's level, mitotic rate, nuclear area and fractal dimension were significant risk factors in univariate Cox regressions. In the multivariate Cox regression, stratified for the presence or absence of metastases at diagnosis, only the Clark level and fractal dimension of the nuclear chromatin were included as independent prognostic factors in the final regression model.In general, a more aggressive behaviour is usually found in genetically unstable neoplasias with a higher number of genetic or epigenetic changes, which on the other hand, provoke a more complex chromatin rearrangement. The increased nuclear fractal dimension found in the more aggressive melanomas is the mathematical equivalent of a higher complexity of the chromatin architecture. So, there is strong evidence that the fractal dimension of the nuclear chromatin texture is a new and promising variable in prognostic models of malignant melanomas.Malignant melanoma is a highly aggressive neoplasia of the skin with a constant and rapidly increasing incidence in the last decades [1] Prognostic factors are currently based on clinical data and morphologic examination (including variables such as tumor thickness, mitotic rate, etc.) [1-3], which are reliable and reproducible. Other prognostic markers, however, which are not yet used in daily practice, might add important information and thus improve prognosis, treatment, and survival. Therefore a search for new prognostic factors is desirable. For this purpose, traditional or new immunohistochemical markers, gene expression arrays, co
Lymph vascular invasion in invasive mammary carcinomas identified by the endothelial lymphatic marker D2-40 is associated with other indicators of poor prognosis
Vanessa FZ Marinho, Konradin Metze, Fernanda SF Sanches, Gislene FS Rocha, Helenice Gobbi
BMC Cancer , 2008, DOI: 10.1186/1471-2407-8-64
Abstract: We selected 123 cases of invasive mammary carcinomas stratified into three subgroups according to axillary lymph node status: macrometastases, micrometastases, and lymph node negative. Lymphatic (LVI) and blood (BVI) vessel invasion were evaluated by H&E and immunohistochemistry using the D2-40 and CD31 antibodies, and related to histologic tumor type and grade, estrogen and progesterone receptors, E-cadherin, Ki67, p53, and Her2/neu expression.LVI was detected in H&E-stained sections in 17/123 cases (13.8%), and in D2-40 sections in 35/123 cases (28.5%) (Kappa = 0.433). BVI was detected in H&E-stained sections in 5/123 cases (4.1%), and in CD31 stained sections in 19/123 cases (15.4%) (Kappa = 0.198). LVI is positively related to higher histologic grade (p = 0.013), higher Ki67 expression (p = 0.00013), and to the presence of macrometastases (p = 0.002), and inversely related to estrogen (p = 0.0016) and progesterone (p = 0.00017) receptors expression.D2-40 is a reliable marker of lymphatic vessels and is a useful tool for lymphatic emboli identification in immunostained sections of breast carcinomas with higher identification rates than H&E. Lymphatic vessel invasion was related to other features (high combined histologic grade, high Ki67 score, negative hormone receptors expression) associated with worse prognosis, probable reflecting a potential for lymphatic metastatic spread and aggressive behavior.Lymphatic vessels are considered the main route by which tumor cells reach axillary lymph nodes [1-3]. Lymphatic vessel invasion (LVI) is known as an independent predictor of lymph node metastases in breast cancer. The diagnosis of LVI is made based on the presence of tumor emboli within vascular channels lined by a single layer of endothelial cells without red blood cells. Lymphatic vessels are flattened channels or open spaces lined by a single layer of endothelial cells whose lumen are sometimes filled with lymphocytes. However, the identification of LVI is diffi
Omeprazole and misoprostol for preventing gastric mucosa effects caused by indomethacin and celecoxib in rats
Cavallini, Míriam Elias;Andreollo, Nelson Adami;Metze, Konradin;Araújo, Marina Raquel;
Acta Cirurgica Brasileira , 2006, DOI: 10.1590/S0102-86502006000300009
Abstract: purpose: to evaluate and to compare macro and microscopically the intense injuries of the gastric mucosa of rats which were caused by nsaids celecoxib and indomethacin and the gastric cytoprotection with omeprazole and misoprostol. methods: the sample is formed by one hundred and fifty wistar rats with average weight 200 g, distributed in four groups, such as: group a, subdivided in groups a1 and a2 - pre-treatment with omeprazole (20 mg/rat) during seven days and on the 8th day - use of nsaids, concerning a1 (20 rats) were given celecoxib (1mg/rat) and a2 (20 rats) were given indomethacin. the group b, subdivided in group b1 and b2 - pre-treatment with misoprostol (20mg/rat) during seven days and on the 8th day use of nsaids, concerning b1 (20 rats) were given celecoxib (1 mg/ rat) and b2 (20 rats) were given indomethacin (12.5 mg/rat). the group c: were not given cytoprotection during seven days, from the 7th to the 8th day - fast of food and water ad libitum, on the 8th day of nsaids use, concerning c1 (20 rats) were given celecoxib, c2 (20 rats) were given indomethacin (12.5 mg/ rat), c3 (20 rats) were given celecoxib (200mg/rato), and group d - control group, concerning 10 rats were observed during seven days ingesting food and water ad libitum. on the 9th day, the stomachs were taken out and were macro and microscopically evaluated for the identification of the gastric injuries. results: on the macroscopic studies, the groups a2, b2 and c2 presented a remarkable high number of injuries for cm2 /animal, respectively 18.55 injuries for cm2 /animal, 16.25 injuries for cm2 /animal and 13.55 injuries for cm2/animal. on the microscopic studies, the percentage of the injured mucosa, presented expressive difference among the groups a1, b1, c1 when compared to the groups a2, b2, c2 (p<0.0001). the average of the length/injury and the average of the depth of the injuries did not present expressive statistics differences among the groups a2, b2 and c2. the average of the
Omeprazole and misoprostol for preventing gastric mucosa effects caused by indomethacin and celecoxib in rats
Cavallini Míriam Elias,Andreollo Nelson Adami,Metze Konradin,Araújo Marina Raquel
Acta Cirurgica Brasileira , 2006,
Abstract: PURPOSE: To evaluate and to compare macro and microscopically the intense injuries of the gastric mucosa of rats which were caused by NSAIDS celecoxib and indomethacin and the gastric cytoprotection with omeprazole and misoprostol. METHODS: The sample is formed by one hundred and fifty Wistar rats with average weight 200 g, distributed in four groups, such as: Group A, subdivided in groups A1 and A2 - pre-treatment with omeprazole (20 mg/rat) during seven days and on the 8th day - use of NSAIDS, concerning A1 (20 rats) were given celecoxib (1mg/rat) and A2 (20 rats) were given indomethacin. The Group B, subdivided in group B1 and B2 - pre-treatment with misoprostol (20mg/rat) during seven days and on the 8th day use of NSAIDS, concerning B1 (20 rats) were given celecoxib (1 mg/ rat) and B2 (20 rats) were given indomethacin (12.5 mg/rat). The Group C: were not given cytoprotection during seven days, from the 7th to the 8th day - fast of food and water ad libitum, on the 8th day of NSAIDS use, concerning C1 (20 rats) were given celecoxib, C2 (20 rats) were given indomethacin (12.5 mg/ rat), C3 (20 rats) were given celecoxib (200mg/rato), and Group D - control group, concerning 10 rats were observed during seven days ingesting food and water ad libitum. On the 9th day, the stomachs were taken out and were macro and microscopically evaluated for the identification of the gastric injuries. RESULTS: On the macroscopic studies, the groups A2, B2 and C2 presented a remarkable high number of injuries for cm2 /animal, respectively 18.55 injuries for cm2 /animal, 16.25 injuries for cm2 /animal and 13.55 injuries for cm2/animal. On the microscopic studies, the percentage of the injured mucosa, presented expressive difference among the groups A1, B1, C1 when compared to the groups A2, B2, C2 (p<0.0001). The average of the length/injury and the average of the depth of the injuries did not present expressive statistics differences among the groups A2, B2 and C2. The average of the edema presented expressive statistics difference among the groups A2 and D; B2 and C2 and between C2 and D (p < 0.05). CONCLUSIONS: The indomethacin on the applied concentration causes a great number of macroscopic and microscopic injuries to gastric mucosa of rats when compared to celecoxib which does not cause lesions. Omeprazole and misoprostol on the applied concentrations do not present macroscopic and microscopic effectiveness on the gastric cytoprotection when applying indomethacin. Considering the microscopic analysis of the average of the edema, the group of animals, which was given
Fractal Characteristics of May-Grünwald-Giemsa Stained Chromatin Are Independent Prognostic Factors for Survival in Multiple Myeloma
Daniela P. Ferro,Monica A. Falconi,Randall L. Adam,Manoela M. Ortega,Carmen P. Lima,Carmino A. de Souza,Irene Lorand-Metze,Konradin Metze
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0020706
Abstract: The use of computerized image analysis for the study of nuclear texture features has provided important prognostic information for several neoplasias. Recently fractal characteristics of the chromatin structure in routinely stained smears have shown to be independent prognostic factors in acute leukemia. In the present study we investigated the influence of the fractal dimension (FD) of chromatin on survival of patients with multiple myeloma.
Molecular characteristics and chromatin texture features in acute promyelocytic leukemia
Mariana R B De Mello, Dulcineia M Albuquerque, Fernanda Pereira-Cunha, Krizzia B Albanez, Katia B B Pagnano, Fernando F Costa, Konradin Metze, Irene Lorand-Metze
Diagnostic Pathology , 2012, DOI: 10.1186/1746-1596-7-75
Abstract: In a prospective study, we analyzed peripheral (PB) leukocyte count, immunophenotype, methylation status of CDKN2B, CDKN2A and TP73; FLT3 and NPM1 mutations besides nuclear chromatin texture characteristics of the leukemic cells. We also examined the relation of these features with patient’s outcome.Among 19 cases, 4 had a microgranular morphology, 7 presented PB leukocytes >10x109/l, 2 had FLT3-ITD and 3 had FLT3-TKD (all three presenting a methylated CDKN2B). NPM1 mutation was not observed. PB leukocyte count showed an inverse relation with standard deviation of gray levels, contrast, cluster prominence, and chromatin fractal dimension (FD). Cases with FLT3-ITD presented a microgranular morphology, PB leukocytosis and expression of HLA-DR, CD34 and CD11b. Concerning nuclear chromatin texture variables, these cases had a lower entropy, contrast, cluster prominence and FD, but higher local homogeneity, and R245, in keeping with more homogeneously distributed chromatin. In the univariate Cox analysis, a higher leukocyte count, FLT3-ITD mutation, microgranular morphology, methylation of CDKN2B, besides a higher local homogeneity of nuclear chromatin, a lower chromatin entropy and FD were associated to a worse outcome. All these features lost significance when the cases were stratified for FLT3-ITD mutation. Methylation status of CDNK2A and TP73 showed no relation to patient’s survival.in APL, patients with FLT3-ITD mutation show different clinical characteristics and have blasts with a more homogeneous chromatin texture. Texture analysis demonstrated that FLTD-ITD was accompanied not only by different cytoplasmic features, but also by a change in chromatin structure in routine cytologic preparations. Yet we were not able to detect chromatin changes by nuclear texture analysis of patients with the FTLD-TKD or methylation of specific genes.Acute promyelocytic leukemia (APL) is a well characterized subtype of acute myeloid leukemia (AML) defined by a specific cytogenetic
The impact of maternal HIV infection on cord blood lymphocyte subsets and cytokine profile in exposed non-infected newborns
Eliane Borges-Almeida, Helaine MBPM Milanez, Maria Marluce S Vilela, Fernanda GP Cunha, Beatriz M Abramczuk, Suiellen C Reis-Alves, Konradin Metze, Irene Lorand-Metze
BMC Infectious Diseases , 2011, DOI: 10.1186/1471-2334-11-38
Abstract: In a prospective, controlled study, 36 mother-child pairs from HIV+ mothers and 15 HIV-uninfected mothers were studied. Hematological features and cytokine profiles of mothers at 35 weeks of pregnancy were examined. Maternal and cord lymphocyte subsets as well as B-cell maturation in cord blood were analyzed by flow cytometry. The non-stimulated, as well as BCG- and PHA-stimulated production of IL2, IL4, IL7, IL10, IL12, IFN-γ and TNF-alpha in mononuclear cell cultures from mothers and infants were quantified using ELISA.After one year follow-up none of the exposed infants became seropositive for HIV. An increase in B lymphocytes, especially the CD19/CD5+ ones, was observed in cord blood of HIV-exposed newborns. Children of HIV+ hard drug using mothers had also an increase of immature B-cells. Cord blood mononuclear cells of HIV-exposed newborns produced less IL-4 and IL-7 and more IL-10 and IFN-γ in culture than those of uninfected mothers. Cytokine values in supernatants were similar in infants and their mothers except for IFN-γ and TNF-alpha that were higher in HIV+ mothers, especially in drug abusing ones. Cord blood CD19/CD5+ lymphocytes showed a positive correlation with cord IL-7 and IL-10. A higher maternal age and smoking was associated with a decrease of cord blood CD4+ cells.in uninfected infants born to HIV+ women, several immunological abnormalities were found, related to the residual maternal immune changes induced by the HIV infection and those associated with antiretroviral treatment. Maternal smoking was associated to changes in cord CD3/CD4 lymphocytes and maternal hard drug abuse was associated with more pronounced changes in the cord B cell line.HIV infection is associated with a complex pattern of changes in the hemopoietic and the immune systems, resulting in abnormalities of peripheral blood (PB) counts and changes in T and B lymphocytes. Decrease of T helper and increase of cytotoxic lymphocytes, profound changes in the cytokine profile and a
Immunophenotyping in Myelodysplastic Syndromes Can Add Prognostic Information to Well-Established and New Clinical Scores
Suiellen C. Reis-Alves, Fabíola Traina, Guilherme Harada, Paula M. Campos, Sara T. O. Saad, Konradin Metze, Irene Lorand-Metze
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0081048
Abstract: Background myelodysplastic syndromes (MDS) are a heterogeneous group of hematopoietic clonal disorders. So, prognostic variables are important to separate patients with a similar biology and clinical outcome. We compared the importance of risk stratification in primary MDS of IPSS and WPSS with the just described revision of IPSS (IPSS-R), and examined if variables obtained by bone marrow immunophenotyping could add prognostic information to any of the scores. Methods In this prospective study of 101 cases of primary MDS we compared the relation of patients’ overall survival with WHO types, IPSS, IPSS-R, WPSS and phenotypic abnormalities of hematopoietic precursors. We examined aberrancies in myelomonocytic precursors and CD34+ cells. Patients were censored when receiving chemotherapy or BM transplantation. Survival analysis was made by Cox regressions and stability of the models was examined by bootstrap resampling. Results median age: 64 years (15–93). WHO types: 2 cases of 5q- syndrome, 7 of RA, 64 of RCDM and 28 of RAEB. In the univariate Cox analysis, increasing risk category of all scores, degree of anemia, higher percentage of BM blasts, higher number of CD34+ cells and their myeloid fractions besides increasing number of phenotypic abnormalities detected were significantly associated with a shorter survival. In the multivariate analysis comparing the three scores, IPSS-R was the only independent risk factor. Comparing WPSS with phenotypic variables (CD34+/CD13+ cells, CD34+/CD13? cells and “total alterations”) the score and “CD34+/CD13+ cells” remained in the model. When IPSS was tested together with these phenotypic variables, only “CD34+/CD13+ cells”, and “total alterations” remained in the model. Testing IPSS-R with the phenotypic variables studied, only the score and “CD34+/CD13+ cells” entered the model. Conclusions Immunophenotypic analysis of myelomonocytic progenitors provides additional prognostic information to all clinical scores studied. IPSS-R improved risk stratification in MDS compared to the former scores.
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