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Search Results: 1 - 10 of 246 matches for " Kimiyo;Barretto "
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The absolute recommendation of chamber Neubauer method for platelets counting instead of indirect methods in severe thrombocytopenic patients
Oliveira, Raimundo Ant?nio Gomes;Takadachi, Maria Mariko;Nonoyama, Kimiyo;Barretto, Orlando Cesar de Oliveira;
Jornal Brasileiro de Patologia e Medicina Laboratorial , 2003, DOI: 10.1590/S1676-24442003000200008
Abstract: accurate and precise platelet counting is crucial for recommending platelets transfusion for thrombocytopenic patients, principally when platelet counts are bellow 30,000/μl. as most laboratories still use the indirect methods for confirming low automated platelet counts, this work compared two indirect methods used in practice (fonio and nosanchunk et al.) with the international committee for standardization in hematology recommended direct method (brecher and cronkite). the obtained data show that the indirect methods present low precision and accuracy, and that the direct method should always be employed in severe thrombocytopenic samples thanks to its high precision.
Is automated platelet counting still a problem in thrombocytopenic blood?
Oliveira, Raimundo Ant?nio Gomes;Takadachi, Maria Mariko;Nonoyama, Kimiyo;Barretto, Orlando César de Oliveira;
Sao Paulo Medical Journal , 2003, DOI: 10.1590/S1516-31802003000100005
Abstract: context: reliable platelet counting is crucial for indicating prophylactic platelet transfusion in thrombocytopenic patients. objective: to evaluate the precision and accuracy of platelet counting for thrombocytopenic patients, using four different automated counters in comparison with the brecher & cronkite reference method recommended by the international committee for standardization in hematology (icsh). type of study: automated platelet counting assessment in thrombocytopenic patients. setting: hematology laboratory, hospital do servidor público estadual de s?o paulo, and the hematology division of instituto adolfo lutz, s?o paulo, sp, brazil. main measurements: brecher & cronkite reference method and four different automated platelet counters. participants: 43 thrombocytopenic patients with platelet counts of less than 30,000/μl results: the advia-120 (bayer), coulter stks, h1 system (technicom-bayer) and coulter t-890 automatic instruments presented great precision and accuracy in relation to laboratory thrombocytopenic samples obtained by diluting blood from normal donors. however, when thrombocytopenic patients were investigated, all the counters except advia (which is based on volume and refraction index) showed low accuracy when compared to the brecher & cronkite reference method (icsh). the advia counter showed high correlation (r = 0.947). however, all counters showed flags in thrombocytopenic samples. conclusion: the brecher & cronkite reference method should always be indicated in thrombocytopenic patients for platelet counts below 30,000 plt /μl obtained in one dimensional counters.
Red cell aspartate aminotransferase saturation with oral pyridoxine intake
Oshiro, Marilena;Nonoyama, Kimiyo;Oliveira, Raimundo Ant?nio Gomes;Barretto, Orlando Cesar de Oliveira;
Sao Paulo Medical Journal , 2005, DOI: 10.1590/S1516-31802005000200004
Abstract: context and objective: the coenzyme of aspartate aminotransferase is pyridoxal phosphate, generated from fresh vegetables containing pyridoxine. vitamin b6-responsive sideroblastic anemia, myelofibrosis and peyronie?s syndrome respond to high pyridoxine doses. the objective was to investigate the oral pyridoxine oral dose that would lead to maximized pyridoxal phosphate saturation of red cell aspartate aminotransferase. design and setting: controlled trial, in hematology division of instituto adolfo lutz. methods: red cell aspartate aminotransferase activity was assayed (before and after) in normal volunteers who were given oral pyridoxine for 15-18 days (30 mg, 100 mg and 200 mg daily). in vitro study of blood from seven normal volunteers was also performed, with before and after assaying of aspartate aminotransferase activity. results: the in vivo study showed increasing aspartate aminotransferase saturation with increasing pyridoxine doses. 83% saturation was reached with 30 mg daily, 88% with 100 mg, and 93% with 200 mg after 20 days of oral supplementation. the in vitro study did not reach 100% saturation. conclusions: neither in vivo nor in vitro study demonstrated thorough aspartate aminotransferase saturation with its coenzyme pyridoxal phosphate in red cells, from increasing pyridoxine supplementation. however, the 200-mg dose could be employed safely in vitamin b6-responsive sideroblastic anemia, myelofibrosis and peyronie?s syndrome treatment. although maximum saturation in circulating red cells is not achieved, erythroblasts and other nucleated and cytoplasmic organelles containing cells certainly will reach thorough saturation, which possibly explains the results obtained in these diseases.
The absolute recommendation of chamber Neubauer method for platelets counting instead of indirect methods in severe thrombocytopenic patients
Oliveira Raimundo Ant?nio Gomes,Takadachi Maria Mariko,Nonoyama Kimiyo,Barretto Orlando Cesar de Oliveira
Jornal Brasileiro de Patologia e Medicina Laboratorial , 2003,
Abstract: Accurate and precise platelet counting is crucial for recommending platelets transfusion for thrombocytopenic patients, principally when platelet counts are bellow 30,000/μl. As most laboratories still use the indirect methods for confirming low automated platelet counts, this work compared two indirect methods used in practice (Fonio and Nosanchunk et al.) with the International Committee for Standardization in Hematology recommended direct method (Brecher and Cronkite). The obtained data show that the indirect methods present low precision and accuracy, and that the direct method should always be employed in severe thrombocytopenic samples thanks to its high precision.
Normal erythrocyte calpain I activity on membrane proteins under near-physiological conditions in patients with essential hypertension
Medeiros, Tereza Maria Dantas de;Ortega, Katia Coelho;Mion Júnior, Décio;Nonoyama, Kimiyo;Barretto, Orlando Cesar de Oliveira;
Sao Paulo Medical Journal , 2002, DOI: 10.1590/S1516-31802002000100002
Abstract: context: it has been reported that the equilibrium between the erythrocyte protease calpain i and its physiological inhibitor calpastatin is disrupted in patients with essential hypertension. objective: to investigate the activity of non-purified calpain i in hemolysates against the erythrocytic membrane proteins, rather than against other substrates. design: evaluation of calpain i red cell activity upon its own physiological substrates in hypertensive patients, in a near-physiological environment. setting: lim-23 and lim-40 of hospital das clinicas of the faculty of medicine of usp. sample: patients with moderate primary hypertension over 21 years of age who were given amlodipine (n:10) and captopril (n:10) for 8 weeks, plus normal controls (n:10). main measurements: red cell membrane proteins were incubated with and without protease inhibitors and with and without calcium chloride and underwent polyacrylamide gel electrophoresis. results: digestion of bands 2.1 and 4.1 was observed, indicating calpain i acitivity. no statistical differences regarding bands 2.1 and 4.1 were observed before treatment, between the controls and the hypertensive patients, either in ghosts prepared without calcium or with increasing concentrations of calcium. nor were statistical differences observed after treatment, between the controls and the patients treated with amlodipine and captopril, or between the patients before and after treatment with both drugs. conclusion: the final activity of non-purified calpain i upon its own physiological substrate, which was the approach utilized in this study, may more adequately reflect what happens in red cells. under such conditions no imbalance favoring calpain i activity increase was observed. the protective factor provided by calpastatin against calpain i activity may diminish under hypertension.
Enzymes and membrane proteins of ADSOL-preserved red blood cells
Leonart, Maria Sueli Soares;Nascimento, Aguinaldo José;Nonoyama, Kimiyo;Pelissari, Cinthia Barbosa;Barretto, Orlando Cesar de Oliveira;
Sao Paulo Medical Journal , 2000, DOI: 10.1590/S1516-31802000000200003
Abstract: context: the preservative solution adsol (adenine, dextrose, sorbitol, sodium chloride and mannitol) maintains red cell viability for blood trans-fusion for 6 weeks. it would be useful to know about its preservation qualities over longer periods. objective: to determine some red cell biochemical parameters for peri-ods of up to 14 weeks in order to determine whether the red cell metabo-lism integrity would justify further studies aiming at increasing red cell preservation and viability. design: biochemical evaluation designed to study red cell preservation. setting: s?o paulo university erythrocyte metabolism referral center. sample: six normal blood donors from the university hospital of the universidade federal do paraná, curitiba, brazil. main measurements: weekly assay of erythrocyte adenosine-5′-triphosphate (atp), 2,3-diphosphoglycerate (2,3dpg), hexokinase (hx), phosphofructokinase (pfk), pyruvate kinase (pk), glucose-6-phosphate dehydrogenase (g-6-pd), 6-phosphogluconic dehydrogenase (6-pgd), glyceraldehyde-3-phosphate dehydrogenase (gapd), glutathione reduc-tase (gr), glutathione peroxidase (gshpx), plasma sodium and potas-sium, blood ph, and membrane proteins of red cells preserved in adsol were studied during storage for 14 weeks storage. results: during adsol preservation, erythrocyte atp concentration decreased 60% after 5 weeks, and 90% after 10 weeks; the ph fell from 6.8 to 6.4 by the 14th week. 2,3-dpg concentration was stable during the first week, but fell 90% after 3 weeks and was exhausted after 5 weeks. by the end of the 5th week, an activity decrease of 16-30% for hx, gapd, gr, g-6-pd and 6-pgd, 35% for pfk and gshpx, and 45% for pk were observed. thereafter, a uniform 10% decay was observed for all enzymes up to the 14th week. the red blood cell membrane pro-teins did not show significant alterations in polyacrylamide gel electro-phoresis (sds-page) during the 14 weeks. conclusion: although the blood viability was shown to be poor from the 6t
Reexamination of standard solar model to the solar neutrino problems
Kimiyo Fukasaku,Takehisa Fujita
Physics , 1997, DOI: 10.1143/PTP.98.1251
Abstract: We examine the calculation of the solar neutrino flux based on the standard solar model (SSM). It is found that the solar neutrino data (KAMIOKANDE experiment) can be well described by the SSM with careful employment of nuclear data of $^7Be (p,\gamma) ^{8} B$. The main point is that the simple-minded product ansatz of Coulomb plus nuclear parts should have a few percent uncertainties which induce the large reduction of the neutrino flux from $^8 B$. Also, if the electron capture of $^7 Be$ inside the sun is suppressed, then the GALLEX experiment can be understood by the SSM calculation.
O imprescindível aporte das ciências sociais para o planejamento e a compreens?o do turismo
Barretto, Margarita;
Horizontes Antropológicos , 2003, DOI: 10.1590/S0104-71832003000200002
Abstract: the aim of this article is to discuss that tourism planning needs the support of social sciences, presenting a quick review of social sciences studies on tourism and raising crucial problems in tourism planning that could be lessened with the help of anthropological and sociological research.
Interfaces entre turismo e migra es: uma abordagem epistemológica
Barretto, Margarita
PASOS : Revista de Turismo y Patrimonio Cultural , 2009,
Abstract: This paper aims to register some interfaces between tourism and migrations as a contribution to tourism theory grounded on studies on mobility. In this first approach, the author begins with three factual statements: first, tourism and migration are two sides of a greater social phenomenon- mobility or geographical displacement- which share motivations and objectives; second, that mobility is a growing phenomenon; third that new information and communication technologies help mobility..
A Survey of Literature on Indigenous Burial Practices and their Archeological Implications
Grace Barretto
Kasarinlan : Philippine Journal of Third World Studies , 2000,
Abstract: Indigenous peoples in the Philippines vary with regards to preparing the body, grave goods, manner and location of burial and post-burial practices. Death beliefs and practices reflect the disposition of their cultures. This survey of written records on indigenous burial practices in the Philippines was divided into four periods -- Spanish, American, post-World War II and contemporary periods. Many of these practices may no longer be examined archeologically due to the disintegration of the material. But the survey allows us to make archeological correlates -- a number of which have been substantiated by excavation sites allover the Philippines, in order to complete or correct an unfinished or misinterpreted record of our history.
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