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Search Results: 1 - 10 of 5807 matches for " Kikue Takebayashi;Cunha "
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Effect of fluoridated varnish and silver diamine fluoride solution on enamel demineralization: pH-cycling study
Delbem, Alberto Carlos Botazzo;Bergamaschi, Maurício;Sassaki, Kikue Takebayashi;Cunha, Robson Frederico;
Journal of Applied Oral Science , 2006, DOI: 10.1590/S1678-77572006000200005
Abstract: objective: in the present investigation, the anticariogenic effect of fluoride released by two products commonly applied in infants was evaluated. methods: bovine sound enamel blocks were randomly allocated to each one of the treatment groups: control (c), varnish (v) and diamine silver fluoride solution (d). the blocks were submitted to ph cycles in an oven at 37oc. next, surface and cross-sectional microhardness were assessed to calculate the percentage loss of surface microhardness (%sml) and the mineral loss (dz). the fluoride present in enamel was also determined. results: f/px10-3 (anova, p<0.05) in the 1st layer of enamel before ph-cycling were (c, v and d): 1.61a; 21.59b and 3.98c. the %smh (kruskal-wallis, p<0.05) were: -64.0a, -45.2b and -53.1c. %dz values (anova, p<0.05) were: -18.7a, -7.7b and -17.3a. conclusion: the data suggested that the fluoride released by varnish showed greater interaction with sound enamel and provided less mineral loss when compared with silver diamine solution.
Neuroendocrine alterations impair enamel mineralization, tooth eruption and saliva in rats
Sassaki Kikue Takebayashi,Delbem Alberto Carlos Botazzo,Santos Otoniel Antonio Macedo dos,Shimabucoro Carlos Eduardo
Pesquisa Odontológica Brasileira , 2003,
Abstract: Neonatal administration of monosodium glutamate (MSG) in rats causes definite neuroendocrine disturbances which lead to alterations in many organ systems. The possibility that MSG could affect tooth and salivary gland physiology was examined in this paper. Male and female pups were injected subcutaneously with MSG (4 mg/g BW) once a day at the 2nd, 4th, 6th, 8th and 10th day after birth. Control animals were injected with saline, following the same schedule. Lower incisor eruption was determined between the 4th and the 10th postnatal days, and the eruption rate was measured between the 43rd and the 67th days of age. Pilocarpine-stimulated salivary flow was measured at 3 months of age; protein and amylase contents were thereby determined. The animals treated with MSG showed significant reductions in the salivary flow (males, -27%; females, -40%) and in the weight of submandibular glands (about -12%). Body weight reduction was only about 7% for males, and did not vary in females. Saliva of MSG-treated rats had increased concentrations of total proteins and amylase activity. The eruption of lower incisors occurred earlier in MSG-treated rats than in the control group, but on the other hand the eruption rate was significantly slowed down. The incisor microhardness was found to be lower than that of control rats. Our results show that neonatal MSG treatment causes well-defined oral disturbances in adulthood in rats, including salivary flow reduction, which coexisted with unaltered protein synthesis, and disturbances of dental mineralization and eruption. These data support the view that some MSG-sensitive hypothalamic nuclei have an important modulatory effect on the factors which determine caries susceptibility.
Dental mineralization and salivary activity are reduced in offspring of spontaneously hypertensive rats (SHR)
Elias, Gracieli Prado;Santos, Otoniel Antonio Macedo dos;Sassaki, Kikue Takebayashi;Delbem, Alberto Carlos Botazzo;Antoniali, Cristina;
Journal of Applied Oral Science , 2006, DOI: 10.1590/S1678-77572006000400008
Abstract: several pathologies have been diagnosed in children of hypertensive mothers; however, some studies that evaluated the alterations in their oral health are not conclusive. this study analyzed the salivary gland activity and dental mineralization of offsprings of spontaneously hypertensive rats (shr). thirty-day-old shr males and wistar rats were studied. the salivary flow was evaluated by injection of pilocarpine, the protein concentration and salivary amylase activity, by the lowry method and kinetic method at 405 nm, respectively. enamel and dentin mineralization of the mandibular incisors was quantified with aid of the microhardness meter. the results were analyzed by the anova or student's t test (p<0.05). it was noticed that the salivary flow rate (0.026 ml/min/100 g ± 0.002) and salivary protein concentration (2.26 mg/ml ± 0.14) of shr offspring were reduced compared to wistar normotensive offspring (0.036 ml/min/100 g ± 0.003 and 2.91 mg/ml ± 0.27, respectively), yet there was no alteration in amylase activity (shr: 242.4 u/ml ± 36.9; wistar: 163.8 u/ml ± 14.1). microhardness was lower both in enamel (255.8 khn ± 2.6) and dentin (59.9 khn ± 0.8) for the shr teeth compared to the wistar teeth (enamel: 328.7 khn ± 3.3 and dentin: 67.1 khn ± 1.0). these results suggest that the shr offspring are more susceptible to development of pathologies impairing oral health, once they presented lesser flow and salivary protein concentration and lower dental mineralization.
Assement of the fluoride concentration and pH in different mouthrinses on the brazilian market
Delbem, Alberto Carlos Botazzo;Sassaki, Kikue Takebayashi;Castro, Alessandra Maia de;Pinto, Leila Maria Cesário Pereira;Bergamaschi, Maurício;
Journal of Applied Oral Science , 2003, DOI: 10.1590/S1678-77572003000400008
Abstract: in this study, the fluoride concentration and ph of 14 commercial brands of mouthrinses were assessed in order to compare them with the values expressed on the labels and with those established by the national sanitary surveillance agency. forty-two (42) products were obtained from three places, with different manufacturing batches. the fluoride concentration was determined in diluted solutions, using a combined specific electrode for fluoride ion (9609 bn orion research) and ion analyzer (290 a orion research). the results showed that 50% of the solutions had statistically significant differences, with higher fluoride concentrations than those expressed on the labels, and the ph ranged from 4.23 to 7.34, but only one of the products registered the ph value on its label.
Evaluation of some properties of fermented milk beverages that affect the demineralization of dental enamel
Lodi, Carolina Simonetti;Sassaki, Kikue Takebayashi;Fraiz, Fabian Calixto;Delbem, Alberto Carlos Botazzo;Martinhon, Cleide Cristina Rodrigues;
Brazilian Oral Research , 2010, DOI: 10.1590/S1806-83242010000100016
Abstract: the aim of this in vitro study was to evaluate the erosive capacity of fermented milk beverages, as well as some of their properties that affect the demineralization of dental enamel (ph, buffering capacity, fluoride, calcium and phosphorus contents). three different batches of 6 commercial brands of fermented milk beverages were analyzed. ph evaluation was accomplished using a potentiometer. the buffering capacity was measured by adding 1 mol l-1 naoh. fluoride concentration was assessed by an ion specific electrode after hexamethyldisiloxane-facilitated diffusion, and calcium and phosphorus concentrations were assessed by a colorimetric test using a spectrophotometer. sixty specimens of bovine enamel were randomly assigned to 6 groups (n = 10). they were exposed to 4 cycles of demineralization in the fermented milk and remineralization in artificial saliva. enamel mineral loss was determined by surface microhardness (%smhc) and profilometric tests. the samples' ph ranged from 3.51 to 3.87; the buffering capacity ranged from 470.8 to 804.2 μl of 1 mol l-1 naoh; the fluoride concentration ranged from 0.027 to 0.958 μgf/g; the calcium concentration ranged from 0.4788 to 0.8175 mgca/g; and the phosphorus concentration ranged from 0.2662 to 0.5043 mgp/g. the %smhc ranged from -41.0 to -29.4. the enamel wear ranged from 0.15 μm to 0.18 μm. in this in vitro study, the fermented milk beverages did not promote erosion of the dental enamel, but rather only a superficial mineral loss.
Analysis of fluoride concentration in mother's milk substitutes
Pagliari, Ana Valéria;Moimaz, Suzely Adas Saliba;Saliba, Orlando;Delbem, Alberto Carlos Botazzo;Sassaki, Kikue Takebayashi;
Brazilian Oral Research , 2006, DOI: 10.1590/S1806-83242006000300016
Abstract: the aim of the present study was to determine the fluoride concentration in some brands of mother's milk substitutes and evaluate the possibility of developing dental fluorosis by consuming these products. the products, all powdered, were divided into 3 groups: infant formulae (group i, n = 7), milk-based (group m, n = 8) and soy-based (group s, n = 3). samples from 3 cans of different batches of each brand were reconstituted in deionized water and analyzed using the specific electrode method, after hexamethyldisiloxane (hmds) facilitated diffusion. the fluoride content (mg f/l) of the products ranged from 0.044 to 0.326 (i), 0.014 to 0.045 (m) and 0.253 to 0.702 (s). there was significant difference in the fluoride content of cans from distinct batches (p < 0.05) in most of the brands. the reconstitution of all products in water with optimal fluoride concentration for consumption during the mineralization phase of the primary teeth could result in daily fluoride intake above 0.07 mg f/kg body weight/day. therefore, the consumption of these products, especially when reconstituted with optimally fluoridated water, could increase the risk of developing dental fluorosis.
Anticaries effect of dentifrices with calcium citrate and sodium trimetaphosphate
Delbem, Alberto Carlos Botazzo;Bergamaschi, Maurício;Rodrigues, Eliana;Sassaki, Kikue Takebayashi;Vieira, Ana Elisa de Mello;Missel, Emilene Macario Coimbra;
Journal of Applied Oral Science , 2012, DOI: 10.1590/S1678-77572012000100017
Abstract: because of the growing concerns regarding fluoride ingestion by young children and dental fluorosis, it is necessary to develop new dentifrices. objective: the aim of this study was to evaluate the effect of dentifrices with calcium citrate (cacit) and sodium trimetaphosphate (tmp) on enamel demineralization. material and methods: enamel blocks (n=70), previously selected through surface hardness analysis, were submitted to daily treatment with dentifrices diluted in artificial saliva and to a ph-cycling model. the fluoride concentration in dentifrices was 0, 250, 450, 550, 1,000 and 1,100 μg f/g. cresttm was used as a positive control (1,100 mg f/g). cacit (0.25%) and tmp (0.25%) were added to dentifrices with 450 and 1,000 μg f/g. surface hardness was measured again and integrated loss of subsurface hardness and fluoride concentration in enamel were calculated. parametric and correlation tests were used to determine difference (p<0.05) and dose-response relationship between treatments. results: the addition of cacit and tmp did not provide a higher fluoride concentration in enamel, however it reduced (p<0.05) mineral loss when compared to other dentifrices; the dentifrice with cacit and tmp and a low fluoride concentration presented similar results when compared to a dentifrice with 1,100 mg f/g (p>0.05). conclusions: dentifrices with 450 and 1,000 μg f/g, cacit and tmp were as effective as a gold standard one.
In vitro assessment of an experimental coat applied over fluoride varnishes
Delbem, Alberto Carlos Botazzo;Brighenti, Fernanda Louren??o;Oliveira, Felipe Alberto Lino;Pessan, Juliano Pelin;Buzalaf, Marília Afonso Rabelo;Sassaki, Kikue Takebayashi;
Journal of Applied Oral Science , 2009, DOI: 10.1590/S1678-77572009000400003
Abstract: objective: the time of contact between the product and enamel surface is important in ensuring the efficacy of fluoride varnishes. thus, some alternatives could avoid fluoride loss to saliva and improve the anticariogenic action of the product. this study evaluated the effect of an experimental coat on the anticariogenic action of fluoride varnishes. material and methods: enamel bovine blocks were selected by evaluating surface microhardness and randomized into five groups (n=24): placebo, duraphattm, duraphattm with coat, duofluoridtm and duofluoridtm with coat. twelve blocks from each group were used to analyze calcium fluoride (caf2) formed on enamel after treatment. the other 12 blocks were subjected to ph cycling for 7 days. the varnishes were kept on enamel for 6 h. next, the percentage change of surface microhardness (%smhc) and mineral loss (δz) were calculated. caf2 retained and fluoride present in the ph-cycled solutions were also measured. results: the use of the coat did not decrease %smhc and δz, but all fluoride varnishes had better results when compared to the placebo (anova and kruskal-wallis, respectively). the values from caf2 formed were higher compared to the values of caf2 retained (non-paired t test, p<0.05). there was a trend to decrease the amount of f in the solutions at the end of ph cycling (kruskal-wallis, p<0.05). conclusions: although the experimental coat increased the formation of caf2 on the enamel surface, it did not significantly improve the anticariogenic action of fluoride varnishes.
Effect of fluoride and gonadal steroid deficiency on enamel and dentin mineralization of female rats
Takeshita, Eliana Mitsue;Iwama, Sayaka;Silva, Tatiana Correa da;Dornelles, Rita Cássia Menegatti;Delbem, Alberto Carlos Botazzo;Sassaki, Kikue Takebayashi;
Journal of Applied Oral Science , 2004, DOI: 10.1590/S1678-77572004000400014
Abstract: the aim of this study was to verify the influence of estrogen deficiency on enamel and dentin microhardness of female rats' incisors, as well as the influence of chronic administration of fluoride on that response. sham or ovx rats drank distilled water or distilled water with 10 ppm fluoride as naf, for 90 days. the upper incisors were removed and the specimens submitted to microhardness analysis of enamel and dentin. the data were compared by variance analysis and tukey's test (p<0.05). there was a significant reduction in the mineralization of crown (7.9% and 8.1%) and root (20.4% and 25.0%) in both groups treated with fluoride or distilled water, respectively and an increase (14.2%) in the mineralization of coronal dentin after ovariectomy. fluoride treatment did not cause reduction in enamel mineralization; however, it reduced dentin mineralization following ovariectomy. it was possible to conclude that estrogens directly or indirectly influence the process of enamel and dentin mineralization of female rats' incisors. the results also indicate that enamel and dentin exhibit different responses to fluoride administration.
The End of Political Islam? A Comparative Analysis of Religious Parties in the Muslim Democracy of Indonesia
Kikue Hamayotsu
Journal of Current Southeast Asian Affairs , 2011,
Abstract: Why do some religious parties survive after adopting a moderate ideological outlook while others do not? The rise and fall of religious parties since the onset of democratic transition in Indonesia has set off an intriguing debate over the role and future of political Islam in electoral politics. This article seeks to explain the diverging – and unexpected – trajectories of the two most prominent religious parties, the National Awakening Party (PKB) and Justice Prosperous Party (PKS) through a close examination of the case of PKB. It emphasizes organizational qualities to advance two claims. First, the decline of the moderate PKB is the result of the permeation of personality-based clientelistic and ascriptive relations as well as lack of party institutionalization. Political survival of the puritanical Islamist PKS, on the other hand, is explained by organizational cohesion achieved through party institutionalization. Second, a disciplined party structure has allowed PKS elites to achieve controversial ideological adjustments. In short, a moderate centralist religious ideology or outlook alone is not sufficient to achieve political survival in the competitive environment of electoral and religious politics.
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