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Search Results: 1 - 10 of 16 matches for " Khurshed Katki "
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Early TBI-Induced Cytokine Alterations are Similarly Detected by Two Distinct Methods of Multiplex Assay
Sanjib Mukherjee,Khurshed Katki,Lee A. Shapiro
Frontiers in Molecular Neuroscience , 2011, DOI: 10.3389/fnmol.2011.00021
Abstract: Annually, more than a million persons experience traumatic brain injury (TBI) in the US and a substantial proportion of this population develop debilitating neurological disorders, such as, paralysis, cognitive deficits, and epilepsy. Despite the long-standing knowledge of the risks associated with TBI, no effective biomarkers or interventions exist. Recent evidence suggests a role for inflammatory modulators in TBI-induced neurological impairments. Current technological advances allow for the simultaneous analysis of the precise spatial and temporal expression patterns of numerous proteins in single samples which ultimately can lead to the development of novel treatments. Thus, the present study examined 23 different cytokines, including chemokines, in the ipsi and contralateral cerebral cortex of rats at 24 h after a fluid percussion injury (FPI). Furthermore, the estimation of cytokines were performed in a newly developed multiplex assay instrument, MAGPIX (Luminex Corp), and compared with an established instrument, Bio-Plex (Bio-Rad), in order to validate the newly developed instrument. The results show numerous inflammatory changes in the ipsi and contralateral side after FPI that were consistently reported by both technologies.
Chemokine CCL2 and its receptor CCR2 are increased in the hippocampus following pilocarpine-induced status epilepticus
Maira L Foresti, Gabriel M Arisi, Khurshed Katki, Andres Monta?ez, Russell M Sanchez, Lee A Shapiro
Journal of Neuroinflammation , 2009, DOI: 10.1186/1742-2094-6-40
Abstract: SE was induced by pilocarpine injection. Control rats were injected with saline instead of pilocarpine. Five days after SE, CCR2 staining in neurons and glial cells was examined using imunohistochemical analyses. The number of CCR2 positive cells was determined using stereology probes in the hippocampus. CCL2 expression in the hippocampus was examined by molecular assay.Increased CCR2 was observed in the hippocampus after SE. Seizures also resulted in alterations to the cell types expressing CCR2. Increased numbers of neurons that expressed CCR2 was observed following SE. Microglial cells were more closely apposed to the CCR2-labeled cells in SE rats. In addition, rats that experienced SE exhibited CCR2-labeling in populations of hypertrophied astrocytes, especially in CA1 and dentate gyrus. These CCR2+ astroctytes were not observed in control rats. Examination of CCL2 expression showed that it was elevated in the hippocampus following SE.The data show that CCR2 and CCL2 are up-regulated in the hippocampus after pilocarpine-induced SE. Seizures also result in changes to CCR2 receptor expression in neurons and astrocytes. These changes might be involved in detrimental neuroplasticity and neuroinflammatory changes that occur following seizures.Chemokines are chemotactic cytokines that direct the migration of cells that express the appropriate chemokine receptor. Chemokine C-C motif ligand 2 (CCL2) is a potent attractant protein for monocytes, therefore being previously denominated monocyte chemoattractant protein-1 (MCP-1). The biological effects of CCL2 are mediated via interactions with its receptor, chemokine C-C motif receptor 2 (CCR2). CCR2 is a G protein-coupled receptor and has also been designated as CD192, CC-CKR-2; CKR2; CMKBR2; MCP-1-R. Upon binding to CCR2, CCL2 regulates the migration and infiltration of monocytes, T-lymphocytes and natural killer cells to regions of inflammation [1-3]. Studies using CCL2 and CCR2 knockout mice have shown that this ligand
Anthrax Toxins Induce Shock in Rats by Depressed Cardiac Ventricular Function
Linley E. Watson, Shu-ru Kuo, Khurshed Katki, Tongyun Dang, Seong Kyu Park, David E. Dostal, Wei-Jen Tang, Stephen H. Leppla, Arthur E. Frankel
PLOS ONE , 2007, DOI: 10.1371/journal.pone.0000466
Abstract: Anthrax infections are frequently associated with severe and often irreversible hypotensive shock. The isolated toxic proteins of Bacillus anthracis produce a non-cytokine-mediated hypotension in rats by unknown mechanisms. These observations suggest the anthrax toxins have direct cardiovascular effects. Here, we characterize these effects. As a first step, we administered systemically anthrax lethal toxin (LeTx) and edema toxin (EdTx) to cohorts of three to twelve rats at different doses and determined the time of onset, degree of hypotension and mortality. We measured serum concentrations of the protective antigen (PA) toxin component at various time points after infusion. Peak serum levels of PA were in the μg/mL range with half-lives of 10–20 minutes. With doses that produced hypotension with delayed lethality, we then gave bolus intravenous infusions of toxins to groups of four to six instrumented rats and continuously monitored blood pressure by telemetry. Finally, the same doses used in the telemetry experiments were given to additional groups of four rats, and echocardiography was performed pretreatment and one, two, three and twenty-four hours post-treatment. LeTx and EdTx each produced hypotension. We observed a doubling of the velocity of propagation and 20% increases in left ventricular diastolic and systolic areas in LeTx-treated rats, but not in EdTx-treated rats. EdTx-but not LeTx-treated rats showed a significant increase in heart rate. These results indicate that LeTx reduced left ventricular systolic function and EdTx reduced preload. Uptake of toxins occurs readily into tissues with biological effects occurring within minutes to hours of serum toxin concentrations in the μg/mL range. LeTx and EdTx yield an irreversible shock with subsequent death. These findings should provide a basis for the rational design of drug interventions to reduce the dismal prognosis of systemic anthrax infections.
Incorporating medical interventions into carrier probability estimation for genetic counseling
Hormuzd A Katki
BMC Medical Genetics , 2007, DOI: 10.1186/1471-2350-8-13
Abstract: We extend Mendelian models to account for medical interventions by accounting for post-intervention disease history through an extra factor that can be estimated from published studies of the effects of interventions. We apply our methods to incorporate oophorectomy into the BRCAPRO model, which predicts a woman's risk of carrying mutations in BRCA1 and BRCA2 based on her family history of breast and ovarian cancer. This new BRCAPRO is available for clinical use.We show that accounting for interventions undergone by family members can seriously affect the mutation carrier probability estimate, especially if the family member has lived many years post-intervention. We show that interventions have more impact on the carrier probability as the benefits of intervention differ more between carriers and non-carriers.These findings imply that carrier probability estimates that do not account for medical interventions may be seriously misleading and could affect a clinician's recommendation about offering genetic testing. The BayesMendel software, which allows one to implement any Mendelian carrier probability model, has been extended to allow medical interventions, so future Mendelian models can easily account for interventions.People who are concerned that their family has a high prevalence of disease may seek counseling to assess their probability of carrying inherited genetic mutations that cause disease [1]. The carrier probability is a crucial component in a person's decision to take a genetic test, to undergo frequent disease screening, or to consider prophylactic medical interventions.To aid such people ("consultands"), genetic counselors use statistical models that predict whether the consultand carries deleterious mutations by using the consultand's reported family history of disease. Mendelian models use Mendel's laws and Bayes's rule to combine family history information with each mutation's known prevalence and penetrance to determine the probability that the c
Quality Study of Rice Husk Ash (RHA) Brick Using Neutron Radiography Technique  [PDF]
Md. Khurshed Alam, Md. Moniruz Zaman, Md. Al Amin
Journal of Building Construction and Planning Research (JBCPR) , 2015, DOI: 10.4236/jbcpr.2015.33013
Abstract: A powerful non-destructive testing (NDT) technique is adopted to study the quality of RHA brick-1 and RHA brick-2. In that case, rice husk ash has been utilized for the preparation of bricks in full replacement of clay. In these studies, homogeneity of elemental distribution, water absorption and size and shape of the pores have been observed. From the studies, it was observed that elemental distribution is very good at various level, large number of porosity is presented with little bit size, initial rate of absorption (IRA) due to first five minutes immersion of water is higher compared to other immersion time (>5 minute), incremental water intrusion area increases very slowly with the increasing immersion time and the water absorption for RHA brick-2 becomes saturated very early than that of the RHA brick-1.
NATURE OF TEACHER-STUDENTS’ INTERACTION IN ELECTRONIC LEARNING AND TRADITIONAL COURSES OF HIGHER EDUCATION- A REVIEW
Sufiana Khatoon MALIK,Fauzia KHURSHED
The Turkish Online Journal of Distance Education , 2011,
Abstract: Present paper explores differential teacher-student interaction in electronic learning (el) and in face to face traditional learning (tl) courses at higher education. After thorough study literature available and getting information from university teachers teaching el and tl courses about the nature of teacher-students interaction in both modes it was found that teacher-students interaction is significantly different in el and tl higher education courses. There are fewer opportunities for developing students’ moral judgment, critical thinking, communication and interpersonal skills in teacher-students interaction in el courses at higher education level. Courses of tl do provide opportunities to students for developing their developing moral judgment, critical thinking, communication and interpersonal skills through face to face interaction with the teacher in direct communication and group discussions on past and current issues along with learning achievement. Arrangement for conducting local educational conference for some e. courses may arrange and participation in such conferences for e. learners may be made mandatory for qualifying a particular degree. El course may be redesigned and practical activities may be incorporate for developing in students’ moral judgment, critical thinking, communication and interpersonal skills.
Quality Study of Automated Machine Made Environmentally Friendly Brick (KAB) Sample Using Film Neutron Radiography Technique  [PDF]
Khurshed Alam, Robiul Islam, Sudipta Saha, Nurul Islam, Syed Azharul Islam
Journal of Building Construction and Planning Research (JBCPR) , 2013, DOI: 10.4236/jbcpr.2013.14015
Abstract: Neutron radiography (NR) technique has been adopted to study the internal structure and quality of the KAB bricks made by Hoffman kiln method. Thermal neutron radiography facility installed at the tangential beam port of 3 MW TRIGA Mark-II Research Reactor, AERE, Savar, Dhaka, Bangladesh is used in the present study. Measurements were made to determine the internal structure and quality of the automated machine made environmentally friendly brick sample. In this case, optical density/gray values of the neutron radiographic images of the sample have been measured. From these measurements, the porosity, water penetrating height, water penetrating behavior, initial rapid absorption of water (IRA), elemental distribution/homogeneity and incremental water intrusion area in the sample have been found. From the observation of different properties, it is seen that, homogeneity of the Hoffman kiln brick KAB is not perfectly homogeneous and contains small internal porosity; the incremental water intrusion area is very poor, and the water penetrating height through the two edges is higher than the middle part; the initial rapid absorption (IRA) rate is also very poor and the water penetrating behavior of the samples is different as like as stair, capillary, wave and zigzag shape. From these points of view, it is concluded that the quality of the environmentally friendly brick KAB is better. The results obtained and conclusion made in this study can only be compared to the properties of bricks produced under similar conditions with similar raw materials.
9-Furfurylidene-2,3-dimethyl-6,7,8,9-tetrahydro-4H-thieno[2′,3′:4,5]pyrimidino[1,2-a]pyridin-4-one
Khurshed A. Bozorov,Burkhon Zh. Elmuradov,Rasul Ya. Okmanov,Bakhodir Tashkhodjaev
Acta Crystallographica Section E , 2010, DOI: 10.1107/s1600536810004101
Abstract: The title compound, C17H16N2O2S, was obtained by condensation of 2,3-dimethylthieno[2′,3′:4,5]pyrimidino[1,2-a]pyridin-4-one with furfural in the presence of sodium hydroxide. One of the methylene groups of the tetrahydropyrido ring is disordered over two positions in a 0.87 (1):0.13 (1) ratio. The thieno[2,3-d]pyrimidin-4-one unit and the furan ring are both planar (r.m.s. deviation = 0.535 ), and coplanar with each other, forming a dihedral angle of 5.4 (1)°. Four weak intermolecular hydrogen bonds (C—H...O and C—H...N) are observed in the structure, which join molecules into a network parallel to (101).
2-Methyl-4-oxo-6,7,8,9-tetrahydrothieno[2′,3′:4,5]pyrimidino[1,2-a]pyridine-3-carboxylic acid
Burkhon Zh. Elmuradov,Khurshed A. Bozorov,Rasul Ya. Okmanov,Bakhodir Tashkhodjaev
Acta Crystallographica Section E , 2011, DOI: 10.1107/s1600536811007902
Abstract: There are two independent molecules in the asymmetric unit of the title compound, C12H12N2O3S. With the exception of the methylene groups, a mean plane fitted through all non-H atoms of each molecule has an r.m.s. deviation of 0.035 for one molecule and 0.120 for the second. In one of the independent molecules, the methylene groups was refined using a disorder model with an occupancy ratio of 0.53:0.47 (14). Each molecule features an intramolecular O—H...O hydrogen bond, which generates an S(7) ring.
Role of sensory nervous system vasoactive peptides in hypertension
Watson R.E.,Supowit S.C.,Zhao H.,Katki K.A.
Brazilian Journal of Medical and Biological Research , 2002,
Abstract: The goal of the present research was to elucidate the roles and mechanisms by which the sensory nervous system, through the actions of potent vasodilator neuropeptides, regulates cardiovascular function in both the normal state and in the pathophysiology of hypertension. The animal models of acquired hypertension studied were deoxycorticosterone-salt (DOC-salt), subtotal nephrectomy-salt (SN-salt), and Nomega-nitro-L-arginine methyl ester (L-NAME)-induced hypertension during pregnancy in rats. The genetic model was the spontaneously hypertensive rat (SHR). Calcitonin gene-related peptide (CGRP) and substance P (SP) are potent vasodilating neuropeptides. In the acquired models of hypertension, CGRP and SP play compensatory roles to buffer the blood pressure (BP) increase. Their synthesis and release are increased in the DOC-salt model but not in the SN-salt model. This suggests that the mechanism by which both models lower BP in SN-salt rats is by increased vascular sensitivity. CGRP functions in a similar manner in the L-NAME model. In the SHR, synthesis of CGRP and SP is decreased. This could contribute to the BP elevation in this model. The CGRP gene knockout mouse has increased baseline mean arterial pressure. The long-term synthesis and release of CGRP is increased by nerve growth factor, bradykinin, and prostaglandins and is decreased by alpha2-adrenoreceptor agonists and glucocorticoids. In several animal models, sensory nervous system vasoactive peptides play a role in chronic BP elevation. In the acquired models, they play a compensatory role. In the genetic model, their decreased levels may contribute to the elevated BP. The roles of CGRP and SP in human hypertension are yet to be clarified.
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