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Search Results: 1 - 10 of 947 matches for " Kerstin Rusch "
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Throwing the dice for the diagnosis of vaginal complaints?
Andreas Schwiertz, David Taras, Kerstin Rusch, Volker Rusch
Annals of Clinical Microbiology and Antimicrobials , 2006, DOI: 10.1186/1476-0711-5-4
Abstract: We evaluated the misjudgement rate of the aetiology of vaginal complaints. A total of 220 vaginal samples from women with a vaginal complaint were obtained and analysed for numbers of total lactobacilli, H2O2-producing lactobacilli, total aerobic cell counts and total anaerobic cell counts including bifidobacteria, Bacteroides spp., Prevotella spp. Additionally, the presence of Atopobium vaginae, Gardnerella vaginalis, Candida spp. and Trichomonas vaginalis was evaluated by DNA-hybridisation using the PCR and Affirm VPIII Microbial Identification Test, respectively.The participating physicians diagnosed Bacterial vaginosis (BV) as origin of discomfort in 80 cases, candidiasis in 109 cases and mixed infections in 8 cases. However, a present BV, defined as lack of H2O2-lactobacilli, presence of marker organisms, such as G. vaginalis, Bacteroides spp. or Atopobium vaginae, and an elevated pH were identified in only 45 cases of the women examined. Candida spp. were detected in 46 cases. Interestingly, an elevated pH corresponded solely to the presence of Atopobium vaginae, which was detected in 11 cases.Errors in the diagnosis of BV and candida vulvovaginitis (CV) were high. Interestingly, the cases of misjudgement of CV (77%) were more numerous than that of BV (61%). The use of Amsel criteria or microscopy did not reduce the number of misinterpretations. The study reveals that the misdiagnosis of vaginal complaints is rather high.The microbiology of the vagina is complex, containing 109 bacterial colony forming units per gram of secretions and potentially dozens of species. It is mainly dominated by members of the genus Lactobacillus, which are capable of H2O2-production and lactic acid, thereby maintaining the generally acidic vaginal pH. Age, phase of the menstrual cycle, sexual activity, contraceptive choice, pregnancy, presence of necrotic tissue or foreign bodies, and use of hygienic products or antibiotics can disrupt this ecosystem. A disturbed vaginal microbiot
Molecular Tools for the Detection of Nitrogen Cycling Archaea
Antje Rusch
Archaea , 2013, DOI: 10.1155/2013/676450
Molecular Tools for the Detection of Nitrogen Cycling Archaea
Antje Rusch
Archaea , 2013, DOI: 10.1155/2013/676450
Abstract: Archaea are widespread in extreme and temperate environments, and cultured representatives cover a broad spectrum of metabolic capacities, which sets them up for potentially major roles in the biogeochemistry of their ecosystems. The detection, characterization, and quantification of archaeal functions in mixed communities require Archaea-specific primers or probes for the corresponding metabolic genes. Five pairs of degenerate primers were designed to target archaeal genes encoding key enzymes of nitrogen cycling: nitrite reductases NirA and NirB, nitrous oxide reductase (NosZ), nitrogenase reductase (NifH), and nitrate reductases NapA/NarG. Sensitivity towards their archaeal target gene, phylogenetic specificity, and gene specificity were evaluated in silico and in vitro. Owing to their moderate sensitivity/coverage, the novel nirB-targeted primers are suitable for pure culture studies only. The nirA-targeted primers showed sufficient sensitivity and phylogenetic specificity, but poor gene specificity. The primers designed for amplification of archaeal nosZ performed well in all 3 criteria; their discrimination against bacterial homologs appears to be weakened when Archaea are strongly outnumbered by bacteria in a mixed community. The novel nifH-targeted primers showed high sensitivity and gene specificity, but failed to discriminate against bacterial homologs. Despite limitations, 4 of the new primer pairs are suitable tools in several molecular methods applied in archaeal ecology. 1. Introduction Archaea have been detected in virtually all types of extreme and moderate environments. They play multiple ecological roles, colonizing certain newly emerging habitats [1, 2], interacting with animals such as corals [3, 4], sponges [5, 6], termites [7], or ruminants, forming part of microbe-microbe symbioses [8–10], and driving numerous processes in the biogeochemical C, N, S, and Fe cycles. In addition to relatively well-studied isolates of extremophilic or methanogenic Archaea, uncultured representatives have been detected by their 16S rRNA genes or by metabolic genes that classify their owners into the guilds of sulfate reducers, diazotrophs, ammonia oxidizers, or methanogens. Despite their widespread occurrence, a mere handful of nonmethanogenic Archaea has been isolated from moderate habitats [11–13]. While such isolates are indispensable for insight into archaeal ecophysiology, they have been recalcitrant to cultivation efforts, so that our current ecological research on mesophilic Archaea largely depends on cultivation-independent methods. Molecular
Active and Healthy Ageing at Work—A Qualitative Study with Employees 55 - 63 Years and Their Managers  [PDF]
Kerstin Nilsson
Open Journal of Social Sciences (JSS) , 2017, DOI: 10.4236/jss.2017.57002
Abstract: The proportion of elderly individuals in the population is increasing in most industrial countries. This demographic change increases the need of more people who work until an older age to maintain the welfare in the states. However, it is not clear if older employees are interested to in generally extend the working life to an older age. It is also not stated if managers in organizations are interested of extending their older employees working life. The overall aim of this study was to examine older employees own experience of their work situation and ageing at work. The aim was also to investigate their managers’ attitude to them as older employees. The investigation was conducted by focusing group interviews with employees aged 55 - 63 years, and with their mangers aged 40 - 63 years. The findings stated that the older employees’ consideration for an extend working life seemed to be based on their health and health problems in relation to their work situation; their personal economy; their managers attitude to them as elderly and the possibility to social inclusion at work; and their possibilities for self-crediting activities at work despite their age. The managers seem to have a positive attitude to some of the older employees’ experience knowledge, if that was in the same direction as their own interest. Otherwise the mangers saw the older employees as problem and obsoleted. The managers were also negative to older employees who got health problems which effect the work production negatively. The older workers in this study described managers’ importance to if they want to work in an extended working life. However, the managers seem not to understand their own importance in this and were not interested to keep all elderly in an extended working life.
The Influence of Work Environmental and Motivation Factors on Seniors’ Attitudes to an Extended Working Life or to Retire. A Cross Sectional Study with Employees 55 - 74 Years of Age  [PDF]
Kerstin Nilsson
Open Journal of Social Sciences (JSS) , 2017, DOI: 10.4236/jss.2017.57003
Abstract: Aim: The aim of this study was to investigate difference between whether individuals think they can work, and want to work until 65 years or not. Methods: A cross/sectional study including survey of 1949 employees aged 55 - 74 years. Results: Working environments were the most significant differences between the groups associated to if the respondents think they can work beyond 65 years or not. Motivation factors were the most significant differences between the groups associated with if the respondents want to work beyond 65 years or not. Conclusions: A satisfying work environment is important to whether people think they can work or not. Nevertheless, whether people want to work is depending on whether the employees are satisfied with the factors that promote their motivation for work. If society wants more people to work until an upper age, it is important to improve both work environmental factors and motivation factors in their work situation.
IRT models with relaxed assumptions in eRm: A manual-like instruction
Psychology Science Quarterly , 2009,
Abstract: Linear logistic models with relaxed assumptions (LLRA) as introduced by Fischer (1974) are a flexible tool for the measurement of change for dichotomous or polytomous responses. As opposed to the Rasch model, assumptions on dimensionality of items, their mutual dependencies and the distribution of the latent trait in the population of subjects are relaxed. Conditional maximum likelihood estimation allows for inference about treatment, covariate or trend effect parameters without taking the subjects' latent trait values into account. In this paper we will show how LLRAs based on the LLTM, LRSM and LPCM can be used to answer various questions about the measurement of change and how they can be fitted in R using the eRm package. A number of small didactic examples is provided that can easily be used as templates for real data sets. All datafiles used in this paper are available from http://eRm.R-Forge.R-project.org/
“Elude” – the making of an impossible game
Doris C. Rusch
E-Beratungsjournal , 2011,
Abstract: Right after the Games for Health Conference in 2009, Doris C. Rusch and Attila Ceranoglu decided to work on a game about depression: A game that actually intends to make the feelings of depression itself experientially tangible to players. They decided to use the internet and virtual reality to reach the digital natives easily, and to raise awareness of the difficulties and problems of depression amongst young people (and therefore become more willing to either seek help for themselves or others). But how to create such a game about depression? How to create a game that would be no fun? Rusch and Ceranoglu found the answers and now Elude“ is done!
An internet-based intervention program for supporting families with prematurely born infants  [PDF]
Birgitta Lindberg, Kerstin ?hrling
Open Journal of Nursing (OJN) , 2012, DOI: 10.4236/ojn.2012.22012
Abstract: The purpose of this paper was to describe the development, planning and implementing of an internet-based intervention program for providing support to families with prematurely born infants. During the families initial stay at home with their infant; families were given access to use of videoconference system from their home to have contact with staff at the neonatal unit, via direct link, throughout twenty-four hours. This program successfully ended, and video-conferencing is incorporated as a tool to support families after coming home. Probably, a variety of factors has contributed to make this program successful, as for example user friendliness and the close collaboration between researchers and staff at the neonatal unit. In conclusion, Information and Communication Technology (ICT) must be taken into consideration in developing upcoming care, thereby making possible a program to extend accessibility to health care.
The Transfer of Knowledge between Younger and Older Employees in the Health and Medical Care: An Intervention Study  [PDF]
Emma Nilsson, Kerstin Nilsson
Open Journal of Social Sciences (JSS) , 2017, DOI: 10.4236/jss.2017.57006
Abstract: In the ageing society more old employees need to stay in working life for longer. However, the society also needs to take care of the increased amount of elderly in a more effective and respectful way. To take care of older employees experience knowledge to make the elderly care and the care employees work situation better and self-crediting to go on in an extended working life, new methods and measures are needed to be developed and evaluated. The overall purpose of this article was to evaluate how the participant in the intervention project experienced the project and its methodology. The intervention project followed three different methods: i) through interviews of the participants and the supervisor; ii) through participant observation; and iii) through an evaluation questionnaire to participants after the project was completed. The intervention project did transfer knowledge between different fields, that are, between generations, between different departments and between the municipality and county. The participation was described as not only a place for reflection and knowledge, but also a protected zone where participants could talk about the kind of problems they would ponder over. Several participants felt that it was important to develop and continue the work that the intervention project set in motion. The participants’ experience was made to be visible and their professional competence has been strengthened. They indicated that there had been an exchange of knowledge and between not only the generations but also the different kinds of work. The organisations also seem to have gained from the project by allowing the participants to unload, become more positive, develop more knowledge of experience, increase their propensity for reflection and get a better consensus between the municipality and county. The intervention project seems to have fulfilled the stated purpose and also the expectations of the participants.
Impacto de diferentes disturbios antrópicos sobre las comunidades de aves de bosques y matorrales de Nothofagus antarctica en el NO Patagónico
Lantschner,María V; Rusch,Verónica;
Ecolog?-a austral , 2007,
Abstract: to be able to plan productive uses of the land compatible with the conservation of the biodiversity, it is essential to evaluate the changes that they cause. the andean patagonic forests undergo different anthropogenic disturbances, like fires, grazing, wood extraction and substitution by exotic coniferous plantations. these activities cause changes on the vegetation and animal communities, and could be affecting the functioning of the forest ecosystems. in argentine patagonia, there is a growing opposition to productive activities, particularly to pine plantations, because of its supposed negative ecological consequences, however their impacts are unknown. the aim of this study was to evaluate in which measure different uses of the vegetation of the basin of foyel river (no argentine patagonia) cause changes on bird communities, especially on the functionally important and the endangered species. birds counts were conducted from november 2004 to march 2005 (summer) using the variable circular-plot method, in twenty four sites of nothofagus antarctica native forests and scrubs with different levels of grazing and wood extraction (closed, semiopened, opened), and in eight exotic plantations of pseudotsuga menziesii and pinus spp. although, the vegetation structure of each site was characterized, to identify structural elements of the forests that could be important for bird habitat. population densities of birds did not differ between vegetation types (25.4 ± 8.4 individuals/ha) associated to different disturbances. bird richness and diversity shannon index), however, were different among the vegetation types, in forestations both were similar to the undisturbed vegetation types, whereas they increased in vegetations with a medium intensity of wood extraction and grazing, and were highest in those sites whit a high intensity of wood extraction and grazing. a canonical correspondence analysis and an analysis of species composition similarity showed that there are two
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