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Search Results: 1 - 10 of 546 matches for " Kensuke Nakata "
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Expression, Purification and Crystallization of Thermostable Mutant of Cutinase Est1 from Thermobifida alba  [PDF]
Kengo Kitadokoro, Shingo Matsui, Ryouhei Osokoshi, Kensuke Nakata, Shigeki Kamitani
Advances in Bioscience and Biotechnology (ABB) , 2018, DOI: 10.4236/abb.2018.95015
Abstract: A double mutantEst1, which is a plastic degrading cutinase-type esterase in Thermobifida alba, has been over-expressed in Escherichia coli. The recombinant protein was purified by a two-step protocol involving immobilized metal affinity chromatography and cation-exchange chromatography, yielding 120 mg of protein per liter of bacterial culture. Crystals have been obtained by using the sitting-drop vapor-diffusion technique. Native diffraction data to 1.37 Å resolution were obtained at the BL44XU beam line of SPring-8 from a flash-frozen crystal at 100 K. The crystals belong to space group C2, with unit-cell parameters a = 127.2 Å, b = 42.1 Å, c = 63.2 Å,
Erratum to “Expression, Purification and Crystallization of Thermostable Mutant of Cutinase Est1 from Thermobifida alba.” [Advances in Bioscience and Biotechnology 9 (2018), 215-223]  [PDF]
Kengo Kitadokoro, Shingo Matsui, Ryouhei Osokoshi, Kensuke Nakata, Uschara Thumarat, Fusako Kawai, Shigeki Kamitani
Advances in Bioscience and Biotechnology (ABB) , 2018, DOI: 10.4236/abb.2018.98025
Abstract: The original online version of this article (Kitadokoro, K., Matsui, S., Osokoshi, R., Nakata, K. and Kamitani, S. (2018) Expression, Purification and Crystallization of Thermostable Mutant of Cutinase Est1 from Thermobifida alba. Advances in Bioscience and Biotechnology, 9, 215-223. https://doi.org/10.4236/abb.2018.95015) unfortunately contains some mistakes. The author wishes to correct the errors.
Behaviors of Crystallization for Osmotic Pressure under Microwave Irradiation  [PDF]
Ryosuke Nakata, Yusuke Asakuma
Journal of Crystallization Process and Technology (JCPT) , 2015, DOI: 10.4236/jcpt.2015.51002
Abstract: We studied chemical garden in order to investigate precipitation behavior for osmotic pressure under microwave irradiation. The salt concentration and microwave irradiation power were varied. Microwave irradiation induced release of osmotic pressure and change of precipitation pattern because polar molecules vibrate and rotate in an electromagnetic field. For example, the width of precipitation increased and the number of rapture of the membrane decreased due to the release of osmotic pressure by the irradiation. Accordingly, microwave irradiation accelerated the diffusion of ionic molecules through the membrane.
Osteoconductivity Control Based on the Chemical Properties of the Implant Surface  [PDF]
Kensuke Kuroda, Masazumi Okido
Journal of Biomaterials and Nanobiotechnology (JBNB) , 2018, DOI: 10.4236/jbnb.2018.91003
Abstract: Metallic materials, such as Ti, Zr, Nb, Ta, and their alloys, and also stainless steels are widely attractive as osteoconductive materials in the dental and orthopedic fields. Ceramics and polymers are also commonly used as biomaterials. However, they do not have high osteoconductivity in their pure form, and surface coatings with bioactive substances, such as hydroxyapatite or TiO2, are needed before implantation into the bone. Many reports claim that the surface chemical properties of implants, in particular, hydrophilicity and hydrophobicity, strongly affect the biological reactions. However, the effect of surface properties on osteoconductivity is not clear. In this review, we focus on the relationship between the surface hydrophilicity of metallic implants and osteoconductivity using in vivo evaluation, and the control of the osteoconductivity is discussed from the viewpoint of protein adsorption in implants.
All about RIKEN Integrated Cluster of Clusters (RICC)
Maho Nakata
International Journal of Networking and Computing , 2012,
Abstract: This is an introduction to the RIKEN's supercomputer RIKEN Integrated Cluster of Clusters (RICC), that has been in operation since August 2009. The basic concept of the RICC is to "provide an environment with high power computational resources to facilitate research and development for RIKEN's researchers". Based on this concept, we have been operating the RICC system as a (i) data analysis environment for experimental researchers, (ii) development environment targeting the next-generation supercomputer; i.e., the "K" computer, and (iii) GPU (graphics processing unit) computers for exploring challenges in developing a future computer environment. The total performance of RICC is 97.94 TFlops, ranking it as the 125th on the Top500 list in Nov. 2011. We prepared four job class accounts, based on the researchers' proposals prior to evaluation by our Review Committee. We also provided backup services to RIKEN's researchers, such as conducting RICC training classes, software installation services, and speed up and visualization support. To encourage affirmative participation and proactive initiation, all the services were free of charge; however, access to RICC was limited to researchers and collaborators of RIKEN. As a result, RICC has been able to maintain a high activity ratio (> 90%) since the beginning of its operation.
Resumption-based big-step and small-step interpreters for While with interactive I/O
Keiko Nakata
Electronic Proceedings in Theoretical Computer Science , 2011, DOI: 10.4204/eptcs.66.12
Abstract: In this tutorial, we program big-step and small-step total interpreters for the While language extended with input and output primitives. While is a simple imperative language consisting of skip, assignment, sequence, conditional and loop. We first develop trace-based interpreters for While. Traces are potentially infinite nonempty sequences of states. The interpreters assign traces to While programs: for us, traces are denotations of While programs. The trace is finite if the program is terminating and infinite if the program is non-terminating. However, we cannot decide (i.e., write a program to determine), for any given program, whether its trace is finite or infinite, which amounts to deciding the halting problem. We then extend While with interactive input/output primitives. Accordingly, we extend the interpreters by generalizing traces to resumptions. The tutorial is based on our previous work with T. Uustalu on reasoning about interactive programs in the setting of constructive type theory.
Endocrine disruptors and environmental impact in Japan
Nakata Kotoko
Cadernos de Saúde Pública , 2002,
Abstract:
Vertex operator for the ultradiscrete KdV equation
Yoichi Nakata
Physics , 2009,
Abstract: We propose an ultradiscrete analogue of the vertex operator in the case of the ultradiscrete KdV equation, which maps N-soliton solutions to N+1-soliton ones.
Vertex operator for the non-autonomous ultradiscrete KP equation
Yoichi Nakata
Physics , 2009, DOI: 10.1088/1751-8113/43/19/195201
Abstract: We propose an ultradiscrete analogue of the vertex operator in the case of the ultradiscrete KP equation--several other ultradiscrete equations--which maps N-soliton solutions to N+1-soliton ones.
A short note on spin pumping theory with Landau-Lifshitz-Gilbert equation under quantum fluctuation; necessity for quantization of localized spin
Kouki Nakata
Physics , 2012, DOI: 10.1142/S0217984912500935
Abstract: We would like to point out the blind spots of the approach combining the spin pumping theory proposed by Tserkovnyak et al. with the Landau-Lifshitz-Gilbert equation; this method has been widely used for interpreting vast experimental results. The essence of the spin pumping effect is the quantum fluctuation. Thus, localized spin degrees of freedom should be quantized, i.e. be treated as magnons not as classical variables. Consequently, the precessing ferromagnet can be regarded as a magnon battery. This point of view will be useful for further progress of spintronics.
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