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Search Results: 1 - 10 of 174627 matches for " Keli Cristine;de-Paris "
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Distribution of erm genes and low prevalence of inducible resistance to clindamycin among staphylococci isolates
Coutinho, Vivian de Lima Spode;Paiva, Rodrigo Minuto;Reiter, Keli Cristine;de-Paris, Fernanda;Barth, Afonso Luis;Machado, Alice Beatriz Mombach Pinheiro;
Brazilian Journal of Infectious Diseases , 2010, DOI: 10.1590/S1413-86702010000600004
Abstract: introduction: resistance to macrolides, lincosamides and streptogramins b (mlsb antibiotics) in staphylococci may be due to modification in ribosomal target methylase encoded by erm genes. the expression of mlsb resistance lead to three phenotypes, namely constitutive resistance (cmlsb), inducible resistance (imlsb), and resistance only to macrolides and streptogramins b (msb). the imlsb resistance is the most difficult to detect in the clinical laboratory. objective: this study investigated the expression of mlsb resistance and the prevalence of the erm genes among 152 clinical isolates of staphylococcus aureus and coagulase-negative staphylococcus (cns) from hospital de clínicas de porto alegre. methods: primary mlsb resistance was detected by the disk diffusion method. isolates with imlsb phenotype were tested by double-disk induction method. all isolates were tested by a genotypic assay, pcr with specific primers. results: a total of 46.7% of staphylococci were positive for cmlsb; 3.3% for imlsb and 3.3% for msb. one or more erm genes were present in 50.1% of isolates. the gene erma was detected in 49 isolates, ermc in 29 and ermb in 3. conclusion: the prevalence of the erma, ermb and ermc genes were 29.6%, 17.1% and 0.66% respectively, and constitutive resistance was the most frequent as compared to the other two phenotypes.
Effect of inhibitory avoidance training on [3H]-glutamate binding in the hippocampus and parietal cortex of rats
Schr?der N.,de-Paris F.,Roesler R.,Medina J.H.
Brazilian Journal of Medical and Biological Research , 2000,
Abstract: Glutamate receptors have been implicated in memory formation. The aim of the present study was to determine the effect of inhibitory avoidance training on specific [3H]-glutamate binding to membranes obtained from the hippocampus or parietal cortex of rats. Adult male Wistar rats were trained (0.5-mA footshock) in a step-down inhibitory avoidance task and were sacrificed 0, 5, 15 or 60 min after training. Hippocampus and parietal cortex were dissected and membranes were prepared and incubated with 350 nM [3H]-glutamate (N = 4-6 per group). Inhibitory avoidance training induced a 29% increase in glutamate binding in hippocampal membranes obtained from rats sacrificed at 5 min (P<0.01), but not at 0, 15, or 60 min after training, and did not affect glutamate binding in membranes obtained from the parietal cortex. These results are consistent with previous evidence for the involvement of glutamatergic synaptic modification in the hippocampus in the early steps of memory formation.
Infusions of AP5 into the basolateral amygdala impair the formation, but not the expression, of step-down inhibitory avoidance
Roesler R.,Vianna M.R.M.,de-Paris F.,Quevedo J.
Brazilian Journal of Medical and Biological Research , 2000,
Abstract: We evaluated the effects of infusions of the NMDA receptor antagonist D,L-2-amino-5-phosphonopentanoic acid (AP5) into the basolateral nucleus of the amygdala (BLA) on the formation and expression of memory for inhibitory avoidance. Adult male Wistar rats (215-300 g) were implanted under thionembutal anesthesia (30 mg/kg, ip) with 9.0-mm guide cannulae aimed 1.0 mm above the BLA. Bilateral infusions of AP5 (5.0 μg) were given 10 min prior to training, immediately after training, or 10 min prior to testing in a step-down inhibitory avoidance task (0.3 mA footshock, 24-h interval between training and the retention test session). Both pre- and post-training infusions of AP5 blocked retention test performance. When given prior to the test, AP5 did not affect retention. AP5 did not affect training performance, and a control experiment showed that the impairing effects were not due to alterations in footshock sensitivity. The results suggest that NMDA receptor activation in the BLA is involved in the formation, but not the expression, of memory for inhibitory avoidance in rats. However, the results do not necessarily imply that the role of NMDA receptors in the BLA is to mediate long-term storage of fear-motivated memory within the amygdala.
Effect of inhibitory avoidance training on [3H]-glutamate binding in the hippocampus and parietal cortex of rats
Schr?der, N.;de-Paris, F.;Roesler, R.;Medina, J.H.;Souza, D.O.;Izquierdo, I.;
Brazilian Journal of Medical and Biological Research , 2000, DOI: 10.1590/S0100-879X2000000200011
Abstract: glutamate receptors have been implicated in memory formation. the aim of the present study was to determine the effect of inhibitory avoidance training on specific [3h]-glutamate binding to membranes obtained from the hippocampus or parietal cortex of rats. adult male wistar rats were trained (0.5-ma footshock) in a step-down inhibitory avoidance task and were sacrificed 0, 5, 15 or 60 min after training. hippocampus and parietal cortex were dissected and membranes were prepared and incubated with 350 nm [3h]-glutamate (n = 4-6 per group). inhibitory avoidance training induced a 29% increase in glutamate binding in hippocampal membranes obtained from rats sacrificed at 5 min (p<0.01), but not at 0, 15, or 60 min after training, and did not affect glutamate binding in membranes obtained from the parietal cortex. these results are consistent with previous evidence for the involvement of glutamatergic synaptic modification in the hippocampus in the early steps of memory formation.
Infusions of AP5 into the basolateral amygdala impair the formation, but not the expression, of step-down inhibitory avoidance
Roesler, R.;Vianna, M.R.M.;de-Paris, F.;Quevedo, J.;Walz, R.;Bianchin, M.;
Brazilian Journal of Medical and Biological Research , 2000, DOI: 10.1590/S0100-879X2000000700014
Abstract: we evaluated the effects of infusions of the nmda receptor antagonist d,l-2-amino-5-phosphonopentanoic acid (ap5) into the basolateral nucleus of the amygdala (bla) on the formation and expression of memory for inhibitory avoidance. adult male wistar rats (215-300 g) were implanted under thionembutal anesthesia (30 mg/kg, ip) with 9.0-mm guide cannulae aimed 1.0 mm above the bla. bilateral infusions of ap5 (5.0 μg) were given 10 min prior to training, immediately after training, or 10 min prior to testing in a step-down inhibitory avoidance task (0.3 ma footshock, 24-h interval between training and the retention test session). both pre- and post-training infusions of ap5 blocked retention test performance. when given prior to the test, ap5 did not affect retention. ap5 did not affect training performance, and a control experiment showed that the impairing effects were not due to alterations in footshock sensitivity. the results suggest that nmda receptor activation in the bla is involved in the formation, but not the expression, of memory for inhibitory avoidance in rats. however, the results do not necessarily imply that the role of nmda receptors in the bla is to mediate long-term storage of fear-motivated memory within the amygdala.
Group B Streptococcus detection: comparison of PCR assay and culture as a screening method for pregnant women
de-Paris, Fernanda;Machado, Alice Beatriz Mombach Pinheiro;Gheno, Tailise Conte;Ascoli, Bruna Maria;Oliveira, Kátia Ruschel Pilger de;Barth, Afonso Luis;
Brazilian Journal of Infectious Diseases , 2011, DOI: 10.1590/S1413-86702011000400004
Abstract: streptococcus agalactiae or group b streptococcus (gbs) is one of the most important causal agents of serious neonatal infections. numerous assays have been evaluated for gbs screening in order to validate a fast and efficient method. the aim of this study was to compare the culture technique (established as the gold standard) with the molecular method of polymerase chain reaction (pcr) with specific primers (atr gene). two hundred and sixty-three samples were analyzed. vaginal samples were collected, according to the centers for disease control and prevention (cdc) recommendations, from women over 35 weeks of pregnancy at hospital de clínicas de porto alegre (hcpa). two different extraction methods were tested in all samples collected. pcr technique yielded 71 (26.99%) positive results. sensitivity and specificity for pcr were 100% and 86.88%, respectively. pcr demonstrated a shorter turnaround time than the culture. the molecular methodology proved to be a useful screening for gbs, allowing effective treatment to be initiated in shorter time to prevent newborn infection.
Comparison of the performance of polymerase chain reaction and pp65 antigenemia for the detection of human cytomegalovirus in immunosuppressed patients
Martiny, Patrícia Borba;de-Paris, Fernanda;Machado, Alice Beatriz Mombach Pinheiro;Mello, Ricardo Obalski de;Senger, Martha Bergman;Corrêa, Maria Clara Medina;Werres Junior, Luiz Carlos;Souza, Carolina Fischinger Moura de;
Revista da Sociedade Brasileira de Medicina Tropical , 2011, DOI: 10.1590/S0037-86822011005000029
Abstract: introduction: human cytomegalovirus (hcmv) is often reactive in latently infected immunosuppressed patients. accordingly, hcmv remains one of the most common infections following solid organ and hemopoietic stem cell transplantations, resulting in significant morbidity, graft loss and occasional mortality. the early diagnosis of hcmv disease is important in immunosuppressed patients, since in these individuals, preemptive treatment is useful. the objective of this study was to compare the performance of the in-house qualitative polymerase chain reaction (pcr) and pp65 antigenemia to hcmv infection in immunosuppressed patients in the hospital de clínicas of porto alegre (hcpa). methods: a total of 216 blood samples collected between august 2006 and january 2007 were investigated. results: among the samples analyzed, 81 (37.5%) were hcmv-positive by pcr, while 48 (22.2%) were positive for antigenemia. considering antigenemia as the gold standard, sensitivity, specificity, positive predictive values and negative predictive values for pcr were 87.5%, 76.8%, 51.8% and 95.5% respectively. conclusions: these results demonstrated that qualitative pcr has high sensitivity and negative predictive value (npv). consequently pcr is especially indicated for the initial diagnosis of hcmv infection. in the case of preemptive treatment strategy, identification of patients at high-risk for hcmv disease is fundamental and pcr can be useful tool.
A combined enrichment/polymerase chain reaction based method for the routine screening of Streptococcus agalactiae in pregnant women
Munari, F.M.;De-Paris, F.;Salton, G.D.;Lora, P.S;Giovanella, P;Machado, A.B.M.P;Laybauer, L.S.;Oliveira, K.R.P.;Ferri, C;Silveira, J.L.S.;Laurino, C.C.F.C;Xavier, R.M.;Barth, A.L;Echeverrigaray, S;Laurino, J.P.;
Brazilian Journal of Microbiology , 2012, DOI: 10.1590/S1517-83822012000100029
Abstract: group b streptococcus (gbs) is the most common cause of life-threatening infection in neonates. guidelines from cdc recommend universal screening of pregnant women for rectovaginal gbs colonization. the objective of this study was to compare the performance of a combined enrichment/pcr based method targeting the atr gene in relation to culture using enrichment with selective broth medium (standard method) to identify the presence of gbs in pregnant women. rectovaginal gbs samples from women at ≥36 weeks of pregnancy were obtained with a swab and analyzed by the two methods. a total of 89 samples were evaluated. the prevalence of positive results for gbs detection was considerable higher when assessed by the combined enrichment/pcr method than with the standard method (35.9% versus 22.5%, respectively). the results demonstrated that the use of selective enrichment broth followed by pcr targeting the atr gene is a highly sensitive, specific and accurate test for gbs screening in pregnant women, allowing the detection of the bacteria even in lightly colonized patients. this pcr methodology may provide a useful diagnostic tool for gbs detection and contributes for a more accurate and effective intrapartum antibiotic and lower newborn mortality and morbidity.
Identification, antimicrobial resistance and genotypic characterization of Enterococcus spp. isolated in Porto Alegre, Brazil
Bender, Eduardo André;Freitas, Ana Lúcia Peixoto de;Reiter, Keli Cristine;Lutz, Larissa;Barth, Afonso Luís;
Brazilian Journal of Microbiology , 2009, DOI: 10.1590/S1517-83822009000300035
Abstract: in the past two decades the members of the genus enterococcus have emerged as important nosocomial pathogens worldwide. in the present study, we evaluated the antimicrobial resistance and genotypic characteristics of 203 enterococcus spp. recovered from different clinical sources from two hospitals in porto alegre, rio grande do sul, brazil. the species were identified by conventional biochemical tests and by an automated system. the genetic diversity of e. faecalis presenting high-level aminoglycoside resistance (hlar) was assessed by pulsed-field gel electrophoresis of chromosomal dna after smai digestion. the e. faecalis was the most frequent specie (93.6%), followed by e. faecium (4.4%). the antimicrobial resistance profile was: 2.5% to ampicillin, 0.5% to vancomycin, 0.5% teicoplanin, 33% to chloramphenicol, 2% to nitrofurantoin, 66.1% to erythromycin, 66.5% to tetracycline, 24.6% to rifampicin, 30% to ciprofloxacin and 87.2% to quinupristin-dalfopristin. a total of 10.3% of the isolates proved to be hlar to both gentamicin and streptomycin (hlrst/ge), with 23.6% resistant only to gentamicin (hlr-ge) and 37.4% only to streptomycin (hlrst). one predominant clonal group was found among e. faecalis hlr-ge/st. the prevalence of resistance among beta-lactam antibiotics and glycopeptides was very low. however, in this study there was an increased number of hlr enterococcus which may be spreading intra and inter-hospital.
High prevalence of methicillin-resistant Staphylococcus aureus with SCCmec type III in cystic fibrosis patients in southern, Brazil
Reiter, Keli Cristine;Machado, Alice Beatriz Mombach Pinheiro;Freitas, Ana Lúcia Peixoto de;Barth, Afonso Luís;
Revista da Sociedade Brasileira de Medicina Tropical , 2010, DOI: 10.1590/S0037-86822010000400008
Abstract: introduction: bacterial colonization of the lungs is the main cause of morbidity in cystic fibrosis (cf). pathogens such as staphylococcus aureus are very well adapted to the pulmonary environment and may persist for years in the same patient. genetic determinants of these bacteria, such as the presence of sccmec have recently emerged as a problem in this population of patients. methods: staphylococcus aureus isolates obtained from different clinical materials coming from cf and non-cf patients attended at a cystic fibrosis reference hospital were compared according to sccmec type and antibiotic susceptibility profile. results: three hundred and sixty-four single-patient staphylococcus aureus isolates were collected, of which 164 (45%) were from cf patients. among the latter, 57/164 (44.5%) were mrsa, and among the non-cf patients, 89/200 (35%) were mrsa. associated pathogens were found in 38 cf patients. all 57 mrsa from cf patients harbored the multiresistant cassette type iii. in contrast, 31/89 mrsa from non-cf patients harbored sccmec type i (35%) and 44/89 harbored type iii (49%). the antibiotic susceptibility pattern was similar between cf and non-cf patients. conclusions: the high prevalence of multiresistant sccmec type iii among cf patients compared with non-cf patients in our institution may make it difficult to control disease progression through antibiotic therapy for promoting the survival of this kind of patient.
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