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Search Results: 1 - 10 of 1062 matches for " Keiko Katayama "
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A Structural Equation Model (SEM) for Pharmacist Competencies in Improving Quality of Life of Cancer Patients: Effect of Missing Values on the SEM  [PDF]
Rieko Takehira, Keiko Murakami, Sirou Katayama, Kenji Nishizawa, Shigeo Yamamura
Pharmacology & Pharmacy (PP) , 2011, DOI: 10.4236/pp.2011.23031
Abstract: Objective: With the goal of improving health-related quality of life (HRQOL) in cancer patients, we previously reported a structural equation model (SEM) of subjected QOL and qualifications of pharmacists, based on a series of questionnaires completed by patients and pharmacists. However, several patients and pharmacists were excluded from the previous study because it was not always possible to obtain all the data intended for collection. In order to reveal the effect of missing data on the SEM, we established SEMs of HRQOL and the competency of pharmacists, using correlation matrices derived by two different statistical methods for handling missing data. Method: Fifteen cancer patients hospitalized for cancer and were receiving opioid analgesics for pain control, and eight pharmacists were enrolled in this study. Each subject was asked four times weekly to answer questions presented in a questionnaire. SEMs were explored using two correlation matrices derived with pair-wise deletion (PD matrix) and list-wise deletion (LD matrix). The final models were statistically evaluated with certain goodness-of-fit criteria. Results: Data were intended to be collected four times weekly for each patient, but there were some missing values. The same SEMs for HRQOL were optimized using both the LD and PD matrices. Although the path diagrams of the SEMs were not identical in the “competency of pharmacists,” the two models suggested that a higher competency of a pharmacist lowered the “severity” of condition and increased the “comfort” of patients, resulting in an increase in the subjected QOL. Conclusion: In collecting data for clinical research, missing values are unavoidable. When the structure of the model was robust enough, the missing data had a minor effect on our SEM of QOL. In QOL research, the LD matrix as well as the PD matrix would be effective, provided the model is sufficiently robust.
Degradation of Aqueous 2,6-Dibromophenol Solution by In-Liquid Dielectric Barrier Microplasma  [PDF]
Shin-Ichiro Kojima, Keiko Katayama-Hirayama, Tetsuya Akitsu
World Journal of Engineering and Technology (WJET) , 2016, DOI: 10.4236/wjet.2016.43042
Abstract: Degradation of 2,6-dibromophenol (2,6-DBP) in the aqueous solution was studied using dielectric barrier discharge in micro-bubbles. Experimental comparison of working gas Ar, N2, O2, and air showed that oxygen and air plasma efficiently decomposed 2,6-DBP to bromide ion, and inorganic carbon. The molecular orbital model was applied in the analysis of the degradation in electrophilic, nucleophilic, and radical reactions.
Dermatomyositis Associated with Docetaxel Use in the Treatment for Ovarian Cancer  [PDF]
Yuta Ito, Yuko Hamada, Keiko Katayama, Hirokazu Uno, Tokio Nakada
Case Reports in Clinical Medicine (CRCM) , 2017, DOI: 10.4236/crcm.2017.610031
Abstract: A 50-year-old woman presented with a 1-week history of rash on the face and hands and difficulty when rising from a sitting position. She underwent an exploratory laparotomy for ovarian cancer 8 months ago. Three months ago, she underwent total hysterectomy, adnexectomy, and retroperitoneal lymph node dissection. Docetaxel and carboplatin were started and administered twice. Physical examination revealed periorbital edema with a purplish appearance; Erythematous lesions on radiodorsal aspects of the proximal interphalangeal and metacarpophalangeal joints were seen. We diagnosed as dermatomyositis on the basis of clinical findings. Interview demonstrated that such rashes were enhanced 1 week after chemotherapy, and got better through following one week. Cutaneous and muscular symptoms got worse two months after her initial visit. Myotonia was found in electromyography, and high density areas on both femoral regions and lower thighs were observed by MRI of skeletal muscle. Scleroderma-like lesions are known as an adverse effect to taxane. In addition, three cases of dermatomyositis due to taxane were reported in Japan. Since dermatomyositis was regarded as one of paraneoplastic syndrome generally, it was not considered as drug-induced. However, clinical course of our case suggests that docetaxel may play more important role in activity of dermatomyositis than ovarian cancer. Seeing patients treated with taxane, therefore, we have to be careful for symptoms suggestive of autoimmune diseases: not only scleroderma but dermatomyositis.
Plasma-Electrolysis of Dinitrophenol in Gas-Liquid Boundary and Interpretation Using Molecular Orbital Theory  [PDF]
Hiroshi Okawa, Hiroki Kuroda, Keiko Hirayama-Katayama, Shin-Ichiro Kojima, Tetsuya Akitsu
World Journal of Engineering and Technology (WJET) , 2019, DOI: 10.4236/wjet.2019.71010
The advanced oxidation of 2,4-dinitrophenol (DNP), 2,5-DNP, and 3,4-DNP in aqueous solution has been investigated using a multi-gas, dielectric barrier discharge. Dielectric barrier discharge was operated in the aqueous solution and gas boundary. The degradation was measured by high performance liquid chromatography (HPLC). The acceleration of the advanced-oxidation has been investigated by the combination of the anion exchange polymer membrane. The result indicated that the degradation pathways involve a rapid detachment of the nitro group and a slow opening of the aromatic-ring. The hydroxyl radical and the excited hydroxyl anion are responsible for the primary attack of the DNP with the production of dihydroxy-nitrobenzenes. The attack of hydroxyl radical occurs at the benzene ring carbon activated by the presence of a phenolic OH group and a nitro group. The result suggested that the reaction is dominated by a pseudo-first order kinetic reaction. The degradation process is interpreted using Molecular Orbital Theory.
Artificial Neural Network Modeling of Quality of Life of Cancer Patients: Relationships between Quality of Life Assessments, as Evaluated by Patients, Pharmacists, and Nurses
Rieko Takehira,Shigeo Yamamura,Keiko Murakami,Sirou Katayama
International Journal of Biomedical Science , 2011,
Abstract: Aim: The purpose of this study was to investigate the difference between the professional perspectives of pharmacists and nurses in Japan with regard to evaluation of the quality of life (QOL) of cancer patients. Methods: A group of cancer hospital inpatients (n=15) were asked to rate the condition of their health and their QOL by filling in a questionnaire. On the same day, a group of pharmacists (n=8) and nurses (n=18) also evaluated patient QOL. Three-layered artificial neural network (ANN) architecture was used to model the relationship between the different QOL evaluations made by patients, pharmacists, and nurses. Results: Although there was no statistical difference between the QOL scores obtained from pharmacists and nurses, the correlation between these scores was weak (0.1188). These results suggest that pharmacists and nurses evaluate the QOL of their patients from different perspectives, based on their respective profession. QOL parameters were modeled with an ANN using the scores, given by patients in answer to questions regarding health-related QOL as input variables. Both the predictive performance of the ANN and the robustness of the optimized model were acceptable. The response surfaces calculated by ANN modeling showed that pharmacists and nurses evaluate patient’s QOL using different information and reasoning, which is likely related to the nature of their contact with the patients. Conclusion: Health professionals evaluate patient QOL from different perspectives, depending on their profession.
Copy-Number Variations Observed in a Japanese Population by BAC Array CGH: Summary of Relatively Rare CNVs
Yasunari Satoh,Keiko Sasaki,Yuko Shimoichi,Keiko Sugita,Hiroaki Katayama,Norio Takahashi
Journal of Biomedicine and Biotechnology , 2012, DOI: 10.1155/2012/789024
Abstract: Copy-number variations (CNVs) may contribute to genetic variation in humans. Reports regarding existence and characteristics of CNVs in a large apparently healthy Japanese cohort are quite limited. We report the data from a screening of 213 unrelated Japanese individuals using comparative genomic hybridization based on a bacterial artificial chromosome microarray (BAC aCGH). In a previous paper, we summarized the data by focusing on highly polymorphic CNVs (in ≥5.0 % of the individuals). However, rare variations have recently received attention from scientists who espouse a hypothesis called “common disease and rare variants.” Here, we report CNVs identified in fewer than 10 individuals in our study population. We found a total of 126 CNVs at 52 different BAC regions in the genome. The CNVs observed at 27 of the 52 BAC-regions were found in only one unrelated individual. The majority of CNVs found in this study were not identified in the Japanese who were examined in the other studies. Family studies were conducted, and the results demonstrated that the CNVs were inherited from one parent in the families.
Characteristics of Highly Polymorphic Segmental Copy-Number Variations Observed in Japanese by BAC-Array-CGH
Norio Takahashi,Yasunari Satoh,Keiko Sasaki,Yuko Shimoichi,Keiko Sugita,Hiroaki Katayama
Journal of Biomedicine and Biotechnology , 2011, DOI: 10.1155/2011/820472
Abstract: Segmental copy-number variations (CNVs) may contribute to genetic variation in humans. Reports of the existence and characteristics of CNVs in a large Japanese cohort are quite limited. We report the data from a large Japanese population. We conducted population screening for 213 unrelated Japanese individuals using comparative genomic hybridization based on a bacterial artificial chromosome microarray (BAC-aCGH). We summarize the data by focusing on highly polymorphic CNVs in ≥5.0% of the individual, since they may be informative for demonstrating the relationships between genotypes and their phenotypes. We found a total of 680 CNVs at 16 different BAC-regions in the genome. The majority of the polymorphic CNVs presented on BAC-clones that overlapped with regions of segmental duplication, and the majority of the polymorphic CNVs observed in this population had been previously reported in other publications. Some of the CNVs contained genes which might be related to phenotypic heterogeneity among individuals.
Accumulating Microglia Phagocytose Injured Neurons in Hippocampal Slice Cultures: Involvement of p38 MAP Kinase
Takahiro Katayama, Hayato Kobayashi, Toshiyuki Okamura, Yuko Yamasaki-Katayama, Tatsuya Kibayashi, Hiroshi Kimura, Keiko Ohsawa, Shinichi Kohsaka, Masabumi Minami
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0040813
Abstract: In this study, microglial migration and phagocytosis were examined in mouse organotypic hippocampal slice cultures, which were treated with N-methyl-D-aspartate (NMDA) to selectively injure neuronal cells. Microglial cells were visualized by the expression of enhanced green fluorescent protein. Daily observation revealed microglial accumulation in the pyramidal cell layer, which peaked 5 to 6 days after NMDA treatment. Time-lapse imaging showed that microglia migrated to the pyramidal cell layer from adjacent and/or remote areas. There was no difference in the number of proliferating microglia between control and NMDA-treated slices in both the pyramidal cell layer and stratum radiatum, suggesting that microglial accumulation in the injured areas is mainly due to microglial migration, not to proliferation. Time-lapse imaging also showed that the injured neurons, which were visualized by propidium iodide (PI), disappeared just after being surrounded by microglia. Daily observation revealed that the intensity of PI fluorescence gradually attenuated, and this attenuation was suppressed by pretreatment with clodronate, a microglia toxin. These findings suggest that accumulating microglia phagocytosed injured neurons, and that PI fluorescence could be a useful indicator for microglial phagocytosis. Using this advantage to examine microglial phagocytosis in living slice cultures, we investigated the involvements of mitogen-activated protein (MAP) kinases in microglial accumulation and phagocytosis. p38 MAP kinase inhibitor SB203580, but not MAP kinase/extracellular signal-regulated kinase inhibitor PD98059 or c-Jun N-terminal kinase inhibitor SP600125, suppressed the attenuation of PI fluorescence. On the other hand, microglial accumulation in the injured areas was not inhibited by any of these inhibitors. These data suggest that p38 MAP kinase plays an important role in microglial phagocytosis of injured neurons.
Adsorption of Urinary Proteins on the Conventionally Used Urine Collection Tubes: Possible Effects on Urinary Proteome Analysis and Prevention of the Adsorption by Polymer Coating
Iwao Kiyokawa,Kazuyuki Sogawa,Keiko Ise,Fumie Iida,Mamoru Satoh,Toshihide Miura,Ryo Kojima,Katsuhiro Katayama,Fumio Nomura
International Journal of Proteomics , 2011, DOI: 10.1155/2011/502845
Abstract: One possible factor determining recovery of trace amount of protein biomarker candidates during proteome analyses could be adsorption on urine tubes. This issue, however, has not been well addressed so far. Recently, a new technical device of surface coating by poly(2-methacryloyloxyethyl phosphorylcholine (MPC)-co-n-butyl methacrylate (BMA)) (poly(MPC-co-BMA)) has been developed mainly to prevent the adsorption of plasma proteins. We assessed whether conventionally used urine tubes adsorb trace amount of urinary proteins and, if any, whether the surface coating by poly(MPC-co-BMA) can minimize the adsorption. Proteinuric urine samples were kept in poly(MPC-co-BMA)-coated and noncoated urine tubes for 15 min and possibly adsorbed proteins and/or peptides onto urine tubes were analyzed by SDS-PAGE, 2-DE, and the MALDI-TOF MS. It was found that a number of proteins and/or peptides adsorb on the conventionally used urine tubes and that surface coating by poly(MPC-co-BMA) can minimize the adsorption without any significant effects on routine urinalysis test results. Although it remains to be clarified to what extent the protein adsorption can modify the results of urinary proteome analyses, one has to consider this possible adsorption of urinary proteins when searching for trace amounts of protein biomarkers in urine. 1. Introduction Urine has now become one of the most attractive biological fluids in clinical proteomics [1, 2]. A number of urinary proteomic studies have been conducted and have revealed urinary biomarker candidates for renal systemic diseases and malignancies of urinary tract [3–5]. Proteomic analysis of urines can be applied to biomarker search in nonrenal diseases as well [6–8]. Although urinary proteome analyses have been conducted by various gel-based and gel-free techniques [9], comprehensive urinary proteome analysis is not an easy task because the urine has very diluted protein concentration with high levels of salts. Sample preparation, processing, and storage for urinary proteomics have been reviewed [10–13]. More recently, an optimized quantitative proteomic strategy for urine biomarker discovery was described [14]. In any event, maximal protein recovery from urine is essential for detecting trace quantities of proteins present in urine for potential biomarker discovery. For this purpose, protein loss during sample preparation should be avoided. One possible factor responsible for loss of trace amounts of urinary proteins could be adsorption to sample tubes, but this issue has not been well addressed so far to our knowledge. Many
Monocytes Infiltrate the Pancreas via the MCP-1/CCR2 Pathway and Differentiate into Stellate Cells
Kazuko Ino, Masahiro Masuya, Isao Tawara, Eri Miyata, Keiko Oda, Yoshiki Nakamori, Kei Suzuki, Kohshi Ohishi, Naoyuki Katayama
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0084889
Abstract: Recent studies have shown that monocytes possess pluripotent plasticity. We previously reported that monocytes could differentiate into hepatic stellate cells. Although stellate cells are also present in the pancreas, their origin remains unclear. An accumulation of enhanced green fluorescent protein (EGFP)+CD45– cells was observed in the pancreases and livers of chimeric mice, which were transplanted with a single hematopoietic stem cell isolated from EGFP-transgenic mice and treated with carbon tetrachloride (CCl4). Because the vast majority of EGFP+CD45– cells in the pancreas expressed stellate cell-associated antigens such as vimentin, desmin, glial fibrillary acidic protein, procollagen-I, and α-smooth muscle actin, they were characterized as pancreatic stellate cells (PaSCs). EGFP+ PaSCs were also observed in CCl4-treated mice adoptively transferred with monocytes but not with other cell lineages isolated from EGFP-transgenic mice. The expression of monocyte chemoattractant protein-1 (MCP-1) and angiotensin II (Ang II) increased in the pancreas of CCl4-treated mice and their respective receptors, C-C chemokine receptor 2 (CCR2) and Ang II type 1 receptor (AT1R), were expressed on Ly6Chigh monocytes isolated from EGFP-transgenic mice. We examined the effect of an AT1R antagonist, irbesartan, which is also a CCR2 antagonist, on the migration of monocytes into the pancreas. Monocytes migrated toward MCP-1 but not Ang II in vitro. Irbesartan inhibited not only their in vitro chemotaxis but also in vivo migration of adoptively transferred monocytes from peripheral blood into the pancreas. Irbesartan treatment significantly reduced the numbers of EGFP+F4/80+CCR2+ monocytic cells and EGFP+ PaSCs in the pancreas of CCl4-treated chimeric mice receiving EGFP+ bone marrow cells. A specific CCR2 antagonist RS504393 inhibited the occurrence of EGFP+ PaSCs in injured mice. We propose that CCR2+ monocytes migrate into the pancreas possibly via the MCP-1/CCR2 pathway and give rise to PaSCs.
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