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OALib Journal期刊

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Genetic Variation in Picea mariana × P. rubens Hybrid Populations Assessed with ISSR and RAPD Markers  [PDF]
Ramya Narendrula, Kabwe Nkongolo
American Journal of Plant Sciences (AJPS) , 2012, DOI: 10.4236/ajps.2012.36088
Abstract: Interspecific hybridization can result in significant shifts in allele frequencies. The objective of the present study was to assess the level of genetic variation in populations of P. mariana × P. rubens hybrids derived from artificial crosses. Progenies from backcross populations created through a series of controlled pollinations among P. mariana and P. rubens trees across the hybridization index were analyzed. Several Inter Simple Sequence Repeat (ISSR) and Random Amplified Polymorphic DNA (RAPD) primers were used to amplify genomic DNA samples from each population. ISSR primers produced from 30% to 52% polymorphic loci. The level of polymorphism was higher with RAPD markers, ranging from 57% to 76%. Overall, the two marker systems generated similar levels of polymorphic loci for P. mariana and P. rubens populations. No significant differences were found among the P. mariana × P. rubens populations analyzed and between the hybrids and the parental populations regardless of the molecular marker used. This confirms the genetic closeness of P. mariana and P. rubens species.
Decrease in Lysine and Tryptophan Content in S2 Inbred Lines from a Quality Protein Maize (QPM) Variety in a Breeding Program  [PDF]
Kabwe Nkongolo, Kankolongo Mbuya
American Journal of Plant Sciences (AJPS) , 2015, DOI: 10.4236/ajps.2015.61021
Abstract: Several countries in Africa, Latin America along with China have incorporated QPM in their Agricultural development plan. A new quality protein maize variety (QPM) was developed by breeders and farmers using the participatory breeding approach in the DR-Congo. It is adapted to all the maize growing regions in the country. Inbred lines from this new variety were produced for further development of maize synthetic populations. The main objective of the present study is to determine the level of amino acid changes in early generations of inbred lines. The results of the study revealed a significant decrease of 33% and 38% of tryptophan in S1 and S2 inbred lines compared to the original parental MUDISHI 3 population, respectively. There was a decrease of 15% of lysine in S2 inbred lines compared to the parental MUDISHI 3. Actually, S2 inbred lines of MUDISHI 3 contain similar level of lysine compared to the genetically improved normal maize (Salongo 2) that is currently released. The development of composite lines is recommended over synthetic populations to maintain the high levels of lysine and tryptophan along with other desirable agronomic characteristics since they involve the intercrossing of open pollinated varieties.
Evaluation of Biological Soil Fertility Management Practices for Corn Production in Oxisols  [PDF]
Mupala G. Muyayabantu, Bakach D. Kadiata, Kabwe K. Nkongolo
American Journal of Plant Sciences (AJPS) , 2012, DOI: 10.4236/ajps.2012.311201
Abstract: Field trials on the management of soil biological fertility with aim to increasing corn production were conducted in a savannah region of the DR-Congo. Three organic matters including fresh biomass of Entada abyssinica, Tithonia diversifolia, Stylosanthes gracilis and a mineral combination of nitrogen and phosphorus (NP) (115-63-0) along with a control (without fertilization) were evaluated for corn crop growth and production. The field trial was a completely randomized design with four replicates. Plant height, basal stem diameter, and yield components were assessed. Irrespective of fertilization treatments and variety, maize showed a similar growth up to 20 days after sowing (DAS), and then two distinct trends were observed. At 60 DAS, plant height and basal diameter were significantly bigger in plots treated with NP, T. diversifolia and E. abyssinica compared to S. gracilis treatment and control (NoF). This pattern was also confirmed with agronomic traits such as cob length, number of kernel per cob, and net grain yield. The local variety was the least productive under any treatment. In general, the response of corn crop to organic and inorganic fertilization showed that the mineral combination (NP) increased the most grain yield and other yield components compared to unfertilized trial, followed by T. divessifolia and E. abyssinica. NP and T. diversifolia treatments increased significantly and equally soil potassium content compared to control and other treatments. Application of T. diversifolia appears a more cost effective approach for small farmers to improving fertility of the oxisol prevailing in Central Africa compared to mineral fertilizers.
Effect of Gamma Irradiation on Morpho-Agronomic Characteristics of Soybeans (Glycine max L.)  [PDF]
Justin Mudibu, Kabwe K. C. Nkongolo, Adrien Kalonji-Mbuyi, Roger V. Kizungu
American Journal of Plant Sciences (AJPS) , 2012, DOI: 10.4236/ajps.2012.33039
Abstract: Mutation breeding in crop plants is an effective approach in improvement of crop having narrow genetic base such as soybean. The main objective of the present study is to determine the effect of different doses of gamma irradiation on different morpho-agronomic characteristics. Agronomic traits that were analyzed included; grain yield, number of pods/plant, number of seeds/plant and weight of 100 seeds and numbers of days to 50% flowering. Morphometric characterization of the descriptive data included plant height, stem diameter, number of leaves/plant, leaflet length, leaflet width, number of ramifications/plant, and pod length and width at 3 lodge stage. The results of the present study revealed that the two gamma irradiation doses used (0.2 kGy and 0.4 kGy) decreased significantly most of agronomic and morphological traits evaluated in M1 populations. Different effects of 0.2 kGy and 0.4 kGy irradiation were observed in M2 populations with significant increase of grain yields and yield components in all the three soybean varieties. In general, a significant decrease or no changes of morphological traits were observed for the two irradiation doses in M2 populations. The levels of changes varied among varieties. Potential high yielding mutants were identified in progenies of irradiated seeds.
Incidence, Severity and Gravity of Cassava Mosaic Disease in Savannah Agro-Ecological Region of DR-Congo: Analysis of Agro-Environmental Factors  [PDF]
Marcel Muengula-Manyi, Kabwe K. Nkongolo, Claude Bragard, Patrick Tshilenge-Djim, Stephan Winter, Adrien Kalonji-Mbuyi
American Journal of Plant Sciences (AJPS) , 2012, DOI: 10.4236/ajps.2012.34061
Abstract: African Cassava mosaic disease (ACMD) is the most severe and widespread disease caused by viruses limiting production of the crop in sub-Saharan Africa. The objective of the present study was to evaluate CMD incidence, severity, and gravity under different agro-environmental conditions. A total of 222 fields were surveyed in 23 different locations. All the farmers grow only local cassava varieties without applications of fertilizers. Overall, mean CMD incidences for all sites surveyed were 58.2% and 51.7%, in 2009 and 2010, respectively. Disease severity ranged from 2.4 to 3.1 on a scale of 1 to 5. Mean disease gravity varied from 29.7% to 62%, in 2010, and 2009, respectively. Detailed analysis of agronomic and environmental revealed no significant association between cassava stand locations, age, land topography and the development of CMD. Likewise intercropping practices and field topping did not affect the development of CMD in all the fields surveyed. There were significant differences in the number of white flies (Bemisia tabaci) per plant in 2009 and 2010, but no significant correlations between the number of B. tabaci per plant and CMD incidence, severity, and gravity was found. In most fields, CMD appears to originate mostly from unhealthy cassava cuttings used for planting.
Growth and Leaf Area Index Simulation in Maize (Zea mays L.) Under Small-Scale Farm Conditions in a Sub-Saharan African Region  [PDF]
Jean-Claude Lukombo Lukeba, Roger Kizungu Vumilia, Kabwe C. K. Nkongolo, Mo?se Lufuluabo Mwabila, Mbungu Tsumbu
American Journal of Plant Sciences (AJPS) , 2013, DOI: 10.4236/ajps.2013.43075
Abstract: Different crop models including MAIZE Ceres, STICS and other approaches have been used to simulate leaf area index (LAI) in maize (Zea mays L.). These modeling tools require genotype-specific calibration procedures. Studies on modeling LAI dynamics under optimal growth conditions with yields close to the yield potential have remained scarce. In the present study, logistic and exponential approaches have been developed and evaluated for the simulation of LAI in maize in a savannah region of the DR-Congo. Data for the development and the evaluation of the model were collected manually by non-destructive method from small farmers’ field. The rate of expansion of the leaf surface and the rate of change of leaf senescence were also simulated. There were measurable variations among sites and varieties for the simulated height of maize plants. At all sites, the varieties with short plants were associated with expected superior performance based on simulation data. In general, the model underestimates the LAI based on observed values. LAI values for the genetically improved maize varieties (Salongo 2, MUS and AK) were greater than those of the unimproved local variety (Local). There were significant differences for K,
Assessing Reactions of Genetically Improved and Local Cassava Varieties to Cassava Mosaic Disease (CMD) Infection in a Savannah Region of the DR-Congo  [PDF]
Marcel Muengula-Manyi, Lyna Mukwa, Kabwe K. Nkongolo, Patrick Tshilenge-Djim, Stephan Winter, Claude Bragard, Adrien Kalonji-Mbuyi
American Journal of Plant Sciences (AJPS) , 2013, DOI: 10.4236/ajps.2013.44101
Abstract:

The responses of eight genetically improved and eight local cassava varieties to cassava mosaic disease (CMD) were evaluated under field conditions at two sites, in Eastern Kasa?, region of the DR-Congo). The varieties were planted using randomized complete block design with three replications. The rate of cuttings sprouted varied significantly from variety to variety and from location to location. Local varieties were severely infected than improved varieties throughout the trial period. In general, the level of CMD incidence for genetically improved varieties was below 15% while it reached 100% for the local cassava varieties six months after planting (MAP). This trend was also observed for the CMD severity and gravity. The mean scores for CMD severity were 2 and 3.6 for genetically improved and local varieties, respectively at 6 MAP. CMD gravity for improved varieties was below 21% for genetically improved varieties and exceeded 85% for local varieties at the end of trials. Area Under the Severity index Progress Curve (AUSiPC) and Area Under Disease Progress Curve (AUDPC) estimates confirmed that improved varieties were moderately infested comparatively to local varieties. Molecular analysis is being conducted to determine the genetic variability and complexity of the cassava mosaic virus strains involved.

Evaluation of Yield and Competition Indices for Intercropped Eight Maize Varieties, Soybean and Cowpea in the Zone of Savanna of South-West RD Congo  [PDF]
Pongi Khonde, Kabongo Tshiabukole, Mbuya Kankolongo, Stefan Hauser, Mumba Djamba, Kizungu Vumilia, Kabwe Nkongolo
Open Access Library Journal (OALib Journal) , 2018, DOI: 10.4236/oalib.1103746
Abstract:
In order to enhance the legumes potential advantages on the cereal yield in intercropping system by nitrogen direct transfer from legume to cereal, an intercropping experiment was conducted between eight maize varieties (07SADVE, 08SADVE 1, 09SADVE F2, Mudishi 1, Mudishi 3, VP0523, ZM538 and Samaru), cowpea (Vigna unguiculata var. H4) and soybean (Glycine max var. Vuangi) during November 2011-February 2012 season at INERA/Mvuazi in the south-western country of DR Congo. The experimental design was a Split plot with four replications, twenty six treatments were applied between maize varieties monocrops (40.000 plant·ha﹣1) and legumes in monoculture (360.000 plant·ha﹣1) and both of sole planting were combined. Evaluation of these soles planting was performed on basis of several intercropping indices (MYE, LER, ATER, RCC, A and CR), the monetary advantage index (MAI), Actual Yield Loss index (AYL) and intercropping index (IA). After data analysis, competitivity indices indicated the higher yields advantages of maize varieties in intercropping, especially Mudishi 3-soybean (MYE = 2836.300 kg·ha﹣1). The equivalent land ratio (LER) varied with 08SADVE 1variety between 0.70 and 1.66 in cowpea and soybean intercrops respectively, the Relative crowding coefficient (RCC) showed yield advantage of all intercrops except with 09SADVE F2, Mudishi 1 and 07SADVE. Aggressivity (A) showed dominance of maize in all intercrops, against the Competitive ratio (CR) showed that the competitivity was stronger on cowpea (from 16.42 to 98.63) than soybean (from 16.12 to 25.70). Actual yield loss (AYL) was negative in all intercrops with cowpea and soybean. Thus, the index of association (IA) informed that the negative values of the different intercrops were due to the maize price (1000 CDF·kg﹣1) and legumes price (1500 CDF·kg﹣1). Finally, the monetary advantage index (MAI = 922.92) and PCA had determined that intercrop with Mudishi 3-soybean as an economic efficiency intercrop than others mixtures.
Analysis of Adaptive Response of Maize (Zea mays) Varieties from DR-Congo to Water Stress  [PDF]
Jean Pierre Kabongo Tshiabukole, Amand Mbuya Kankolongo, Gertrude Pongi Khonde, Antoine Mumba Djamba, Roger Kizungu Vumilia, Kabwe Nkongolo
American Journal of Plant Sciences (AJPS) , 2017, DOI: 10.4236/ajps.2017.89153
Abstract: Maize production in tropical Africa is often negatively affected by drought. The main objectives of the present study were to 1) analyze the impact of water stress on the agro-morphological performance of two varieties of Quality Protein Maize (QPM) compared to two normal maize varieties and 2) assess their adaptive response in contrasting water environments. Agro-morphological responses to water deficiency of maize (Zea mays L.) were assessed in controlled experiments using four maize varieties, two normal maize (Zm725 and Mus1) and two quality protein maize (Mudishi1 and Mudishi3) varieties. They were subjected to three water regimes (100%, 60%, 30% water retention capacity) at the beginning of the bloom stage, using a Fischer block design with four replications. Significant differences (p < 0.05) among varieties, water regimes and their interactions for plant growth and production parameters were observed. Reduction of water supply to plants caused changes in aerial and underground plant growth. Plant stem height, foliar expansion, and root system development characterizing vegetative growth showed variation in varietal response to water regimes. Mus1 (normal maize variety) was the best adapted to variations in water regimes because they developed an important root volume to adapt to the effects of water deficit while maintaining their morphological and productive characteristics.
Review on Rockburst Theory and Types of Rock Support in Rockburst Prone Mines  [PDF]
Eugie Kabwe, Yiming Wang
Open Journal of Safety Science and Technology (OJSST) , 2015, DOI: 10.4236/ojsst.2015.54013
Abstract: As the depth of mining increases, the occurrence of seismic events is becoming a more common phenomenon causing serious problems regarding to the stability and safety of mines. However, seismicity and the accompanying rockburst phenomena are not a well-defined and well-understood in underground excavations in these times. Efforts to clearly explain the mechanisms are underway. This paper is an overview on the mechanism of rockburst and supports applicably in rockburst prone excavations, predicted classification scales for damage on rock surfaces and rock supports. Current design methods for support systems are reviewed, which are mostly dependent on practical approaches and are geared towards static support design. Based on this, the current review focuses on ground supports under dynamic conditions.
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