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Search Results: 1 - 10 of 175704 matches for " Juracy de Freitas;Tadei "
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Variability and genetic differentiation among Anopheles (Ano.) intermedius Chagas, 1908 and Anopheles (Ano.) mattogrossensis Lutz & Neiva, 1911 (Diptera: Culicidae) from the Brazilian Amazon
Santos, Joselita Maria Mendes dos;Rodriguez, Gloria Alicia Diaz;Maia, Juracy de Freitas;Tadei, Wanderli Pedro;
Memórias do Instituto Oswaldo Cruz , 2005, DOI: 10.1590/S0074-02762005000600006
Abstract: anopheles (anopheles) intermedius and anopheles (ano.) mattogrossensis are brazilian anopheline species belonging to the scarcely studied anopheles subgenus. few studies have been done on the genetic differentiation of these species. both species have been found infected by plasmodium and are sympatric with other anopheline species from the nyssorhynchus subgenus. eighteen enzymatic loci were analyzed in larval specimens of an. intermedius and an. mattogrossensis aiming to estimate the variability and genetic differentiation between these species. an. mattogrossensis population showed higher genetic variability (p = 44.4 and ho = 0.081 ± 0.031) than that of an. intermedius (p = 33.3 and ho = 0.048 ± 0.021). most analyzed loci showed genotypic frequencies according to hardy-weinberg equilibrium, except for lap1 and lap2 in an. intermedius, and est1 and pgm loci in an. mattogrossensis. the genetic distance between these species (d = 0.683) was consistent with the inter-specific values reported for anopheles subgenus. we verified that the polymorphism and heterozygosity percentile values found in both species and compared to those in the literature, showed no relation between the level of isozyme variability and geographical distribution. the low variability found in these two species is probably more related to the niche they occupy than to their geographic distribution.
Genetic variability among Anopheles species belonging to the Nyssorhynchus and Anopheles subgenera in the Amazon region
Moroni, Raquel Borges;Maia, Juracy de Freitas;Tadei, Wanderli Pedro;Santos, Joselita Maria Mendes dos;
Revista da Sociedade Brasileira de Medicina Tropical , 2010, DOI: 10.1590/S0037-86822010000400015
Abstract: introduction: isoenzymatic analyses were performed involving species of the nyssorhynchus and anopheles subgenera in order to estimate the intra and interspecies genetic variability. methods: mosquitoes were caught at different localities in the amazon region. the collection and rearing of mosquitoes in the laboratory followed specific protocols. for the genetic variability analyses, the technique of horizontal electrophoresis on starch and starch-agarose gel with appropriate buffer systems was used. the alloenzyme variation was estimated using the biosys-1 software. results: out of the 13 loci, eight were polymorphic. anopheles nuneztovari presented the largest number of alleles per locus, while the smallest number was detected in anopheles marajoara from macapá. the largest number of polymorphic loci was found for anopheles marajoara from maruanum and the smallest for anopheles benarrochi (guayará mirim). anopheles darlingi (macapá) presented the greatest heterozygosity (ho = 0.167 ± 0.071), while the lowest heterozygosity (ho = 0.045 ± 0.019) was observed in anopheles intermedius (pacoval) of the subgenus anopheles. wright's f coefficient revealed considerable genetic structuring between the populations of anopheles darlingi (fst = 0.110) and between the populations of anopheles marajoara (fst = 0.082). conclusions: considering all the species studied, the genetic distance ranged from 0.008 to 1.114. the greatest distance was between anopheles mattogrossensis and anopheles oswaldoi, while the smallest was between the anopheles benarrochi populations.
Isoenzymatic variability among five Anopheles species belonging to the Nyssorhynchus and Anopheles subgenera of the Amazon region, Brazil
Santos, Joselita Maria Mendes dos;Maia, Juracy de Freitas;Tadei, Wanderli Pedro;Rodriguez, Gloria Alícia Diaz;
Memórias do Instituto Oswaldo Cruz , 2003, DOI: 10.1590/S0074-02762003000200014
Abstract: an isoenzymatic comparative analysis of the variability and genetics differentiation among anopheles species was done in populations of an. (nys.) intermedius and an. (ano.) mattogrossensis of the anopheles subgenus, and of an. darlingi, an. albitarsis and an. triannulatus of the nyssorhynchus subgenus, with the aim of detecting differences between both subgenera and of estimating the degree of genetic intere specific divergence. samples from macapá, state of amapá and janauari lake, near manaus, state of amazonas, were analyzed for eight isoenzymatic loci. analysis revealed differences in the average number of alleles per locus (1.6-2.3) and heterozygosity (0.060-0.284). however, the proportion of polymorphic loci was the same for an. (nys.) darlingi, an. (nys.) triannulatus and an. (ano.) mattogrossensis (50%), but differed for an. (nys.) albitarsis (62.5%) and an. (ano.) intermedius (25%). only the idh1 (p > 0.5) locus in all species studied was in hardy-weinberg equilibrium. the fixation index demonstrated elevated genetic structuring among species, based on values of fst = 0.644 and genetic distance (0.344-0.989). genetic difference was higher between an. (nys.) triannulatus and an. (ano.) intermedius (0.989) and smaller between an. (nys.) albitarsis sensu lato and an. (nys.) darlingi (0.344). the data show interspecific genetic divergence which differs from the phylogenetic hypothesis based on morphological characters.
Isoenzymatic variability among five Anopheles species belonging to the Nyssorhynchus and Anopheles subgenera of the Amazon region, Brazil
Santos Joselita Maria Mendes dos,Maia Juracy de Freitas,Tadei Wanderli Pedro,Rodriguez Gloria Alícia Diaz
Memórias do Instituto Oswaldo Cruz , 2003,
Abstract: An isoenzymatic comparative analysis of the variability and genetics differentiation among Anopheles species was done in populations of An. (Nys.) intermedius and An. (Ano.) mattogrossensis of the Anopheles subgenus, and of An. darlingi, An. albitarsis and An. triannulatus of the Nyssorhynchus subgenus, with the aim of detecting differences between both subgenera and of estimating the degree of genetic intere specific divergence. Samples from Macapá, State of Amapá and Janauari Lake, near Manaus, State of Amazonas, were analyzed for eight isoenzymatic loci. Analysis revealed differences in the average number of alleles per locus (1.6-2.3) and heterozygosity (0.060-0.284). However, the proportion of polymorphic loci was the same for An. (Nys.) darlingi, An. (Nys.) triannulatus and An. (Ano.) mattogrossensis (50%), but differed for An. (Nys.) albitarsis (62.5%) and An. (Ano.) intermedius (25%). Only the IDH1 (P > 0.5) locus in all species studied was in Hardy-Weinberg equilibrium. The fixation index demonstrated elevated genetic structuring among species, based on values of Fst = 0.644 and genetic distance (0.344-0.989). Genetic difference was higher between An. (Nys.) triannulatus and An. (Ano.) intermedius (0.989) and smaller between An. (Nys.) albitarsis sensu lato and An. (Nys.) darlingi (0.344). The data show interspecific genetic divergence which differs from the phylogenetic hypothesis based on morphological characters.
Enzymatic variability in Aedes aegypti (Diptera: Culicidae) populations from Manaus-AM, Brazil
Fraga, Elmary da Costa;Santos, Joselita Maria Mendes dos;Maia, Juracy de Freitas;
Genetics and Molecular Biology , 2003, DOI: 10.1590/S1415-47572003000200011
Abstract: eighteen enzymatic loci were analysed in aedes aegypti populations from four neighbourhoods in the city of manaus. the analyses showed that the downtown population was the most polymorphic (p = 55.6%) with higher observed and expected mean heterozygosities (ho = 0.152 ± 0.052; he = 0.174 ± 0.052, respectively). the least variability was detected in the coroado and cidade nova populations, both with polymorphism of 44.4%. the latter population presented the least observed heterozygosity (ho = 0.109 ± 0.037). wright's f statistics showed that the mean value of fis was higher than that of fst (fis = 0.164 > fst = 0.048), and from analysis of molecular variance (amova) it was found that 95.12% of the variability is found within populations indicating a certain intra-population differentiation possibly of the microgeographic structure resulting from some barrier in the random coupling. although the four populations were similar genetically (d = 0.003 to 0.016), the 4.88% differentiation was significant.
Enzymatic variability in Aedes aegypti (Diptera: Culicidae) populations from Manaus-AM, Brazil
Fraga Elmary da Costa,Santos Joselita Maria Mendes dos,Maia Juracy de Freitas
Genetics and Molecular Biology , 2003,
Abstract: Eighteen enzymatic loci were analysed in Aedes aegypti populations from four neighbourhoods in the city of Manaus. The analyses showed that the Downtown population was the most polymorphic (p = 55.6%) with higher observed and expected mean heterozygosities (Ho = 0.152 ± 0.052; He = 0.174 ± 0.052, respectively). The least variability was detected in the Coroado and Cidade Nova populations, both with polymorphism of 44.4%. The latter population presented the least observed heterozygosity (Ho = 0.109 ± 0.037). Wright's F statistics showed that the mean value of Fis was higher than that of Fst (Fis = 0.164 > Fst = 0.048), and from analysis of molecular variance (AMOVA) it was found that 95.12% of the variability is found within populations indicating a certain intra-population differentiation possibly of the microgeographic structure resulting from some barrier in the random coupling. Although the four populations were similar genetically (D = 0.003 to 0.016), the 4.88% differentiation was significant.
Constituintes químicos do caule de Spathelia excelsa (rutaceae) e atividade frente a Aedes aegypti
Freitas, Aline Carvalho de;Lima, Maria da Paz;Ferreira, Antonio Gilberto;Tadei, Wanderli Pedro;Pinto, Ana Cristina da Silva;
Química Nova , 2009, DOI: 10.1590/S0100-40422009000800016
Abstract: phytochemical investigation from the stems of spathelia excelsa (rutaceae) collected in amazonas yielded deacetylspathelin (1), 7,8-dimethoxyflindersine (2), new glabretal-type triterpenoid 3β-angeloyl-21,24-epoxy-7α, 21α, 23α, 25-tetrahydroxy-4α, 4β, 8β, 10β-tetramethyl-25-dimethyl-14, 18-cyclo-5α, 13α, 14α, 17α-cholestane (3), in addition to the known steroids β-sitosterol and stigmasterol. their structures were established on the basis of spectral data. the compounds 1 and 3 were assayed on aedes aegypti (larvicidal and adulticidal activities and compound 3 exhibited larvicidal properties with lc50 of 4,8 μg/ml.
Rela es interpessoais em famílias com portador da doen a de Alzheimer
Lima, Liliane Dias de,Marques, Juracy Cunegatto
Psico , 2007,
Abstract: A Doen a de Alzheimer (DA) é uma desordem neurodegenerativa progressiva e incapacitante que se caracteriza pela deteriora o cognitiva e da memória acompanhada da perda de autonomia, conseqüente à impossibilidade do desempenho das atividades diárias. Acompanhada por uma variedade de sintomas neuropsiquiátricos e distúrbios comportamentais leva o idoso acometido à total dependência. Em geral, o cuidado destes destes pacientes que se tornam dependentes progressivamente recai sobre as famílias provocando mudan as nas rela es interpessoais intrafamiliares. Este trabalho, apoiando-se na teoria psicossocial de desenvolvimento, tem, como principal objetivo, apresentar algumas considera es que podem ser úteis para a compreens o das rela es interpessoais que ocorrem nas famílias de portadores de Doen a de Alzheimer. Segundo essa perspectiva teórica, para enfrentar tal problemática, cada membro da família será desafiado a menter a própria integridade do ego, para que possa oferecer melhores cuidados a(o) progenitor(a) sem, contudo, alterar o estilo próprio de vida.
Adolescência, sexualidade e mídia: uma breve revis?o da literatura nacional e internacional
Miguel, Raquel de Barros Pinto;Toneli, Maria Juracy Filgueiras;
Psicologia em Estudo , 2007, DOI: 10.1590/S1413-73722007000200009
Abstract: this study had as objective identify to the existing production concerning the subjects adolescence, sexuality and media. to carry it out, surveys in international and national databases had been done, using related describing terms to the subject. in the joined references the subjects more investigated by its authors had been identified, being between them: informative rule of the media, the sexual content propagated by the media, its influence in the sexuality of the adolescent and relation between media and the preservation of the dominant paradigm. the media vehicles more explored in the studies are the television and the magazines. it was possible also to identify authors of relevance, as much in international as the national scope, as well as books and periodics that are reference in the area. it was evidenced the lack of production about of this thematic one, mainly when compared with the number of works approaching the subjects adolescence and sexuality, in special in national scope.
Screening of plants found in the State of Amazonas, Brazil for activity against Aedes aegypti larvae
Pohlit, Adrian Martin;Quinard, Etienne Louis Jacques;Nunomura, Sergio Massayoshi;Tadei, Wanderli Pedro;Hidalgo, Ari de Freitas;Pinto, Ana Cristina da Silva;Santos, Elba Vieira Mustafa dos;Morais, Sabrina Kelly Reis de;Saraiva, Rita De Cássia Guedes;Ming, Lin Chau;Alecrim, Alexandre Mascarenhas;Ferraz, Alfeu de Barros;Pedroso, Andreza Cristiana da Silva;Diniz, Elisangela Vieira;Finney, Ellen Kathryn;Gomes, Erika de Oliveira;Dias, Hercules Bezerra;Souza, Katiuscia dos Santos de;Oliveira, Laura Cristina Pereira de;Don, Luciana de Castro;Queiroz, Maria Mireide Andrade;Henrique, Marycleuma Campos;Santos, Mirian dos;Lacerda Júnior, Orivaldo da Silva;Pinto, Patrícia de Souza;Silva, Suniá Gomes;Gra?a, Yara Rodrigues;
Acta Amazonica , 2004, DOI: 10.1590/S0044-59672004000100012
Abstract: ethanol, methanol and water extracts representing mostly native plant species found in the amazon region were prepared, respectively, by maceration, continuous liquid-solid extraction and infusion, followed by evaporation and freeze-drying. the freeze-dried extracts were tested for lethality toward aedes aegypti larvae at test concentrations of 500 mg / ml. in general, methanol extracts exhibited the greatest larvicidal activity. the following 7 methanol extracts of (the parts of) the indicated plant species were the most active, resulting in 100% mortality in a. aegypti larvae: tapura amazonica poepp. (root), piper aduncum l. (leaf and root), p. tuberculatum jacq. (leaf, fruit and branch). and simaba polyphylla (cavalcante) w.w. thomas (branch).
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