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Search Results: 1 - 10 of 7576 matches for " Juliana;Siqueira "
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A proposal for the quality control of Tanacetum parthenium (feverfew) and its hydroalcoholic extract
Chaves, Juliana Siqueira;Da Costa, Fernando Batista;
Revista Brasileira de Farmacognosia , 2008, DOI: 10.1590/S0102-695X2008000300009
Abstract: in this study, we present a proposal for the physical and chemical quality control of the powder of the species t. parthenium (feverfew) and its hydroalcoholic extract obtained by percolation. the sesquiterpene lactone parthenolide, the main active compound of this plant, was quantified by hplc and its content was found to be 0.49% in the powder and 1.06% in the extract. the total content of flavonoids, determined by uv spectroscopy, was found to be 0.54% in the powder and 1.05% in the hydroalcoholic extract. santin, the main flavonoid of this species, was isolated and further identified in the extract by hplc. since parthenolide can also be found in other asteraceae species, the analysis of santin is important to certify the authenticity of the plant material. the results confirmed the authenticity of the plant material and the efficiency of the extraction procedure.
The spectator on the images meantime of The ballad of sexual dependency O espectador no entretempo das imagens de The ballad of sexual dependency
Juliana Salles Siqueira,Carlos Magno Mendona
Galáxia , 2012,
Abstract: By attempting to the intermedial character Nan Goldins several works - and specifically of her slide show The ballad of sexual dependency (1979-), this paper intends to indicate the peculiar way in which the artist dialectize images of her personal collection, mobilizing the spectator for an active work in the meantime and intervals of the photographs displayed. The consideration of the intermedial aspects of her work and the return to the Didi Hubermans concept of dialectical images (1998) allow us to think about the process by which the photographer gives legibility to the pictures of her archive and invokes the spectators to draw their own set of associations and dissociations. We retake, at this point of our argument, to the theme of spectators emancipation as raised by Jacques Rancire (2008). Ao atentar para o carter intermedial formador de vrias obras de Nan Goldin e, em especfico, do diaporama The ballad of sexual dependency (1979), o presente artigo procura indicar o modo peculiar como a artista dialetiza as imagens de seu acervo pessoal, mobilizando o espectador para um trabalho ativo nos intervalos e entretempos das fotografias exibidas. A considerao dos aspectos intermiditicos de sua obra e o retorno ao conceito de imagens dialticas proposto por Didi-Huberman (1998) permitem-nos compreender o processo pelo qual a fotgrafa d legibilidade a suas fotografias de arquivo, bem como convoca o espectador a elaborar seu prprio jogo de associaes e dissociaes. Retomamos, neste ponto de nosso argumento, o tema da emancipao do espectador conforme suscitado por Jacques Rancire (2008).
Do original à cópia: a voz dos estilistas sobre a cria o da moda (From the original to the copy: the fashion designers’ voice about fashion creation)
Juliana Godoi,Rafael Siqueira de Guimar?es
Revista Emancipa??o , 2010,
Abstract: Resumo: Nos últimos anos, mudan as cruciais vêm acontecendo na cena do luxo e da moda em geral. Objetivou-se neste trabalho entender as cria es realizadas por estilistas no ambito da moda. Utilizando a história oral, buscou-se contextualizar as cria es realizadas por profissionais, elaborando perspectivas diante dessa produ o e considerando esse trabalho sob diversos aspectos, incluindo-o como uma “manifesta o artística” das mudan as sociais e como uma composi o de diversos estilos, influenciada sobre diversos aspectos, e que acompanha o tempo, hábitos, costumes e comportamentos de determinado grupo. Verifica-se, com este estudo, que a produ o de moda tem aspectos muito singulares, referentes à individualidade do estilista, ao momento em que se vive e às influencias as quais aquele está submetido, bem como suas experiências. Abstract: In recent years, crucial changes have been happening in the scene of luxury and fashion in general. This study aims at understanding the creations of fashion designers in the fashion field. Using verbal history, the study sought to contextualize the creations of the fashion professionals, elaborating perspectives regarding their production and considering several aspects of their work, regarding it as an “artistic manifestation” of the social changes, and also as a mixture of various styles influenced by various aspects; moreover, as something that follows the tendencies, habits, customs and behaviors of a determined group. Furthermore, this study verifies that the fashion production has a series of very singular aspects which can refer to the individuality of a given fashion designer; to the time in which he lives; and to the influences that surround him, as well as his experiences.
Mau desempenho escolar: uma vis?o atual
Siqueira, Cláudia Machado;Gurgel-Giannetti, Juliana;
Revista da Associa??o Médica Brasileira , 2011, DOI: 10.1590/S0104-42302011000100021
Abstract: this study aims to develop a comprehensive review on the issue of poor school performance for professionals in both health and education areas. it discusses current aspects of education, learning and the main conditions involved in underachievement. it also presents updated data on key aspects of neurobiology, epidemiology, etiology, clinical presentation, comorbidities and diagnosis, early intervention and treatment of the major pathologies comprised. it is a comprehensive, non-systematic literature review on learning, school performance, learning disorders (dyslexia, dyscalculia and dysgraphia), attention deficit / hyperactivity disorder (adhd) and developmental coordination disorder (dcd). poor school performance is a frequent problem faced by our children, causing serious emotional, social and economic issues. an updated view of the subject facilitates clinical reasoning, accurate diagnosis and appropriate treatment.
Cellular Alterations Induced by Candida albicans RC Nosodes: an in vitro Study
Fortune Homsani,Gleyce Moreno,Camila Siqueira,Juliana Grechi
International Journal of High Dilution Research , 2012,
Abstract: Introduction: Candidiasis is an opportunist infection, caused by yeast of the genus Candida, which emerges as one of the main causes of systemic infections in hospitalized patients. Candida albicans is the most common causing agent of these infections. According to the Brazilian Homeopathic Pharmacopeia[1], nosodes are medicines compounded from chemically undefined biological products. Living nosodes are prepared using the etiologic agent of an illness in its infective form, were first developed by Brazilian physician Roberto Costa (RC). Roberto Costa ¢a a ¢s research indicated that living nosodes present a higher capability to stimulate the host ¢a a ¢s immunological system [2]. Aim: This study aims to evaluate cellular alterations induced in C. albicans yeasts and RAW 264-7 macrophages by Candida albicans RC. Methodology: To prepare Candida albicans RC, one part of C. albicans infective yeast suspension (108 cell/ml) was diluted in 9 parts of sterile distilled water and submitted to 100 mechanical succussions. This process was successively repeated to the potencies of 12x and 30x1. Water 30x was prepared by the same technique, as control. The cell viability of C. albicans previously treated with nosodes in both potencies and respective controls was evaluated using the samples at the concentration of 10% (V/V), in a volume of 1ml, distributed in 1-3 days. The viability of the yeast cells was analyzed by MTT (3-(4,5-dimetiltiazol-2-il)-2,5-difeniltetrazolic) (5mg/ml) assay [3] and by Propidium Iodide (PI) incorporation methods. Additionally, using macrophages RAW 264-7 as a cell model, Nitric Oxide (NO) production and cell viability were also evaluated. For this, the following protocol of cell treatment was employed: on each experimental day, RAW 264-7 cells were treated 4 times (4 stimuli) with RC nosode 30x at the concentration of 10% (V/V). Results: The nosodes (12x and 30x) did not present cytotoxic effects on macrophage cells (n=1), or on C. albicans yeasts (n=2), as detected by MTT and PI methods. Moreover, no statistically significant differences on NO production were detected among the experimental groups (n=6). Conclusion: Preliminary results of in vitro assays indicate that nosodes (12x and 30x) do not alter mitochondrial activity or cell viability of C. albicans. Similarly, treatment by RC nosodes does not seem to alter NO release and mitochondrial activity of RAW macrophages. New experiments are being performed to confirm these preliminary data.
Evaluation of the genotoxic and mutagenic potentials of a living nosode compounded with Influenza A virus
Juliana Paiva,Camila Siqueira,Carla Holandino,Alvaro Leitao
International Journal of High Dilution Research , 2011,
Abstract: Background: The influenza virus has been responsible for contagious respiratory diseases with high mortality rates [1]. Some drugs have been used to treat human influenza. However, these drugs cause many common side effects and induce the appearance of resistant viral strains [2]. The impact caused by the influenza virus has motivated the development of new approaches for the prevention and control of influenza [3]. Therefore, a new homeopathic medicine was developed using, as a starting point, the infectious influenza virus [4]. This belongs to a group called living nosodes [5]. However, its mutagenic and genotoxic potentials, especially when used in low dilutions, has not yet been evaluated and it is important because this biotherapic is prepared from living microorganisms. Different methods can be used to detect mutagenic and genotoxicic effects. Aims: This study aims to evaluate the genotoxic and mutagenic potentials of influenza A living nosode at different homeopathic potencies. Methodology: 1 ml of purified viral suspension was diluted in 9 ml of sterile distilled water. This sample was submitted to 100 mechanical succussions (approximately 3 Hz), using Autic Brazilian machine, originating the first dilution, named decimal (1x). 1 ml of this solution was diluted in 9 ml of solvent and was submitted to 100 sucussions, generating biotherapic 2x. This procedure was successively repeated, according to Brazilian Homeopathic Pharmacopoeia, to obtain the biotherapic 30x [6]. By the same technique, water vehicle was prepared until 30x potency to be used as control. All samples were prepared in sterile and under aseptic conditions, using laminar flow cabinet, class II, and were stored in the refrigerator (8 oC). The samples 1x, 6x, 12x, 18x, 24x and 30x and water 30x (vehicle control) were analysed by: the Inductest, which assesses the ability of physical or chemical agents to promote lysogenic induction as a reflection of damage in DNA molecules in lysogenic bacteria, and the Ames test, which uses indicator strains of Salmonella typhimurium, sensitive to substances that can induce different types of mutation. Results: The Inductest showed no decrease in the survival fraction of the bacteria used, and no increase in the formation of lysogenic induction, in any tested potency. The same profile was obtained after the Ames test, with similar results to negative control. Conclusion: We can conclude that this living nosode compounded with Influenza A virus is not able to induce DNA damage in prokaryotic cells. This result permits us to conclude that pati
Compara??o de três protocolos distintos para extra??o de RNA de amostras fixadas em formalina e emblocadas em parafina
Gouveia, Gisele Rodrigues;Ferreira, Suzete Cleusa;Sabino, Ester Cerdeira;Siqueira, Sheila Aparecida Coelho;Pereira, Juliana;
Jornal Brasileiro de Patologia e Medicina Laboratorial , 2011, DOI: 10.1590/S1676-24442011000600012
Abstract: introduction: formalin fixed paraffin embedded (ffpe) tissues are an important sample source for retrospective studies. despite its ability to preserve proteins and cell morphology, formalin hinders molecular biology tests once it fragments and chemically modifies nucleic acids, particularly rna. objective: to compare the efficiency of three different rna extraction protocols for gene expression analysis of ffep tissues. material and methods: rna was extracted from ffpe samples of human lymph by means of ambion and arcturus bioscience kits and the classical trizol method. after extraction, rna was quantified and tested for amplification through real time polymerase chain reaction (rt-pcr) using glyceraldehydes-3 phosphate dehydrogenase (gapdh) endogenous gene primers. discussion/conclusion: all the protocols produced sufficient and adequate amounts of total rna. however, only protocols using arcturus and ambion kits generated suitable rna for pcr amplification.
Development of enteric coated tablets from spray dried extract of feverfew (Tanacetum parthenium L: )
Chaves, Juliana Siqueira;Da Costa, Fernando Batista;Freitas, Luís Alexandre Pedro de;
Brazilian Journal of Pharmaceutical Sciences , 2009, DOI: 10.1590/S1984-82502009000300024
Abstract: tanacetum parthenium (feverfew) is an herb that is commercialized worldwide as a therapeutic treatment for migraine. its pharmacological effect is mainly due to the presence of the sesquiterpene lactone parthenolide as well as of flavonoids. so far, there are no studies on standardization of pre-formulations or phytomedicines containing this herb. the present study aimed at developing a pre-formulation using a standardized spray-dried extract of feverfew and further designing and standardizing enteric coated tablets. in this work, the spray-dried extract of feverfew was evaluated for its parthenolide, santin and total flavonoid content, parthenolide solubility, particle size, tapped density, hygroscopicity, angle of repose and moisture content. tablets containing the spray-dried extract were tested for their average weight, friability, hardness, and disintegration time. the total flavonoid and parthenolide contents in the spray-dried extract were 1.31 % and 0.76% w/w, respectively. the spray-dried extract presented consistent pharmacotechnical properties and allowed its tableting by direct compression. tablet properties were in accordance with the proposed specifications. the procedures described herein can be used to prepare and evaluate pre-formulations of feverfew with adequate properties for the development of a high-quality phytomedicine.
Efeitos da inje??o de solu??o bicarbonatada de ácido acetilsalicílico em mucosa colorretal de coelhos, com vistas a aplica??o no preparo pré-operatório do cólon
Ioriatti, Enrico Salom?o;Rodrigues, Maria A. M.;Siqueira, Juliana Menezes;Hossne, Rogério Saad;
Revista Brasileira de Coloproctologia , 2007, DOI: 10.1590/S0101-98802007000400012
Abstract: background: the implantation of viable exfoliated intraluminal tumour cells is the major cause of local recurrence in colorectal cancer. 11-28 therefore, the bowel lumen wash with a tumoricidal agent has been recommended. 11-28 in previous study we observe that acetylsalicylic acid solution cause neoplastic cell death in vitro.31 purpose: assess the local effect of acetylsalicylic acid solution on the colonic mucosa of rabbits, in order to use this agent in the bowel lumen wash. methods: 20 rabbits were used. a vascular clamp was placed on the distal colon, followed by the instillation per rectum of 50 ml of acetylsalicylic acid solution or saline solution, according to the group. the euthanasia was performed immediately or later according to the group. results: the acetylsalicylic acid solution doesn't cause any injury on the colonic mucosa of rabbits. conclusion: the use of acetylsalicylic acid solution in the bowel lumen wash seems clinically feasible.
Comportamento de amostras ambientais e clínicas de Cryptococcus neoformans frente a fungicidas de uso agron?mico e ao fluconazol
Drummond, Elislene Dias;Reim?o, Juliana Quero;Dias, Amanda Latercia Tranches;Siqueira, Ant?nio Martins de;
Revista da Sociedade Brasileira de Medicina Tropical , 2007, DOI: 10.1590/S0037-86822007000200012
Abstract: the activity of azole fungicides for agronomical use (epoxiconazole, difenoconazole and cyproconazole) was evaluated in comparison with the therapeutic antifungal agent fluconazole, on 23 environmental samples of cryptococcus neoformans var neoformans isolated from pigeon feces that were collected from farms with agricultural practices using azole compounds, and on 11 clinical samples isolated from patients with cryptococcosis. sensitivity tests were performed using the agar dilution technique. the minimum inhibitory concentration capable of inhibiting 50% of the environmental isolates (mic 50) was 6.00μg/ml to epoxiconazole, 1.00μg/ml for difenoconazole, 2.00μg/ml for cyproconazole and 64.00μg/ml for fluconazole. among the clinical isolates the mic 50 values were 2.00μg/ml, 0.38μg/ml, 1.00μg/ml and 16.00μg/ml for epoxiconazole, difenoconazole, cyproconazole and fluconazole, respectively. the mic 50 values for environmental isolates were greater than the mic 50 values for clinical isolates. in our study, in relation to the same antifungal agent, the environmental samples presented significantly different behaviour in relation to the clinical samples (p<0.05). differences in the mic values (p<0.05) presented by fluconazole and the other antifungal agents for agronomical use, both in the environmental isolates and in the clinical isolates, were also observed.
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