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Search Results: 1 - 10 of 409540 matches for " Juliana M.;Nascimento "
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Comparison between Two Ethanolic Solutions for 3’-Deoxy-3’-[18F]Fluorothymidine Elution  [PDF]
Leonardo T. C. Nascimento, Marina B. Silveira, Soraya M. Z. M. D. Ferreira, Juliana B. Silva
Advances in Chemical Engineering and Science (ACES) , 2017, DOI: 10.4236/aces.2017.71003
Abstract: 3’-Deoxy-3’-[18F]Fluorothymidine—[18F]FLT is a Positron Emission Tomography (PET) tracer which has been used for noninvasive assessment of proliferation activity in several types of cancer. During the past few years, some novel approaches for [18F]FLT synthesis have been developed, mainly focused on optimization of reaction conditions and purification methods. The present study reports the use of two different eluents in the final step of [18F]FLT production and the evaluation of its effect on radiochemical yield and product quality. The first eluent evaluated was water: ethanol (90:10, v/v), commercially available, and the second was NaCl 0.9% (saline): ethanol (92:8, v/v). The mean of the corrected radiochemical yields corresponded to 27% ± 7% for elution with water and ethanol and to 23% ± 3% for elution with saline and ethanol, which could indicate that the eluent solutions have similar elution strength. Besides, quality control results were in accordance with the requirements and demonstrated that there was no significant difference between both formulations. Considering that pharmaceutical preparations containing ethanol should be preferentially diluted with saline to avoid hemolysis, the eluent saline:ethanol (92:8, v/v) was chosen for [18F]FLT extraction and final
Environmental pollutants and stroke-related hospital admissions
Nascimento, Luiz Fernando Costa;Francisco, Juliana B.;Patto, Marielle Beatriz R.;Antunes, Angélica M.;
Cadernos de Saúde Pública , 2012, DOI: 10.1590/S0102-311X2012000700010
Abstract: some effects of environmental pollution on human health are known, especially those affecting the respiratory and cardiovascular systems. the current study aimed to estimate these effects on the production of hospital admissions for stroke. this was an ecological study using hospital admissions data in s?o josé dos campos, s?o paulo state, brazil, with diagnosis of stroke, from january 1, 2007, to april 30, 2008. the target pollutants were particulate matter, sulfur dioxide, and ozone. use of a poisson linear regression model showed that same-day exposure to particulate matter was associated with hospitalization for stroke (rr = 1.013; 95%ci: 1.001-1.025). an increase of 10μg/m3 in this pollutant increased the risk of hospitalization by 12% (rr = 1.137; 95%ci: 1.014-1.276). in the multi-pollutant model, it was thus possible to identify particulate matter as associated with hospitalization for stroke in a medium-sized city like s?o josé dos campos.
Production of cuticle-degrading proteases by Nomuraea rileyi and its virulence against Anticarsia gemmatalis
Nunes, Ana Rita Fonseca;Martins, Juliana Nascimento;Furlaneto, Márcia Cristina;Barros, Neiva Monteiro de;
Ciência Rural , 2010, DOI: 10.1590/S0103-84782010005000149
Abstract: nomuraea rileyi represents an important natural control agent of anticarsia gemmatalis preventing populations from reaching economic threshold levels in soybean. during the processes of host infection, entomopathogenic fungi produce extracellular proteases, which degrade the host cuticle and are suggested to be virulence determinants. it was examined the production of subtilisin-like (pr1) and trypsin-like (pr2) proteases in two strains (nr458 and cg434) of n. rileyi and its possible role in the process of pathogenicity to this caterpillar. fungal growth was performed in a mineral medium containing nitrate, and supplemented with the cuticle or exuviae from a. gemmatalis, or with the non-cuticular substrate casein. in medium containing nitrate as sole nitrogen source, no detectable pr1-like activity occurred in the culture supernatants of the two fungal strains. however, both strains of n. rileyi produced pr1-like protease in all medium amended with exogenous nitrogen source, and it was highly expressed in the presence of insect cuticle. pr2-like activity was significantly inferior to pr1-like activity and it was detected only in some of the media culture and incubation periods tested. in the nr458 culture supernatant the highest activity was observed in medium containing nitrate as nitrogen source. correlation analysis between the percentage of a. gemmatalis mortality in bioassays and pr1-like protease activity of strain nr458 suggests a positive correlation for these variables.
Inibi??o da corrente de cálcio tipo L por tramadol e enanti?meros em miócitos cardíacos de ratos
Medei, Emiliano;Raimundo, Juliana M.;Nascimento, José Hamilton M.;Trachez, Margarete M.;Sudo, Roberto T.;Zapata-Sudo, Gisele;
Arquivos Brasileiros de Cardiologia , 2011, DOI: 10.1590/S0066-782X2011005000091
Abstract: background: tramadol is a centrally acting analgesic, whose mechanism of action involves opioid-receptor activation. previously, we have shown that tramadol and its enantiomers had a negative inotropic effect on the papillary muscle in which the (+)-enantiomer is more potent than (-)- and (±)-tramadol. objective: in this study, we investigated the effects of tramadol and its enantiomers on l-type calcium current (ica-l). results: tramadol (200 μm) reduced the peak amplitude of ica-l at potentials from 0 to +50 mv. at 0 mv, ica-l was reduced by 33.7 ± 7.2%. (+)- and (-)-tramadol (200 μm) produced a similar inhibition of ica-l, in which the peak amplitude was reduced by 64.4 ± 2.8% and 68.9 ± 5.8%, respectively at 0 mv (p > 0.05). tramadol, (+)- and (-)-tramadol shifted the steady-state inactivation of ica-l to more negative membrane potentials. also, tramadol and (+)-tramadol markedly shifted the time-dependent recovery curve of ica-l to the right and slowed down the recovery of ica-l from inactivation. the time constant was increased from 175.6 ± 18.6 to 305.0 ± 32.9 ms (p < 0.01) for tramadol and from 248.1 ± 28.1 ms to 359.0 ± 23.8 ms (p < 0.05) for (+)-tramadol. the agonist of μ-opioid receptor damgo had no effect on the ica-l. conclusion: the inhibition of ica-l induced by tramadol and its enantiomers was unrelated to the activation of opioid receptors and could explain, at least in part, their negative cardiac inotropic effect.
Maxadilan (MAX) - Proteína salivar de Lutzomyia longipalpis: detec??o de anticorpos antiMAX em leishmaniose tegumentar americana (LTA) e express?o gênica e protéica de MAX em Lutzomyia neivai
Aires, Juliana Machado;Chociay, Maria Fernanda;Nascimento, Margarida M. P.;Figueiredo, José Fernando de C.;Roselino, Ana Maria F.;
Anais Brasileiros de Dermatologia , 2005, DOI: 10.1590/S0365-05962005001000012
Abstract: background: the protein max, salivary component of lutzomyia longipalpis, vector of calazar or systemic leishmaniasis, has been used as vaccine for experimental tegumentar leishmaniasis, which vasodilatory and immunomodulatory functions are described. objectives: our purpose was to detect antibodies antimax in sera samples from patients with atl and to verify the genetic and protein expression of max in l. neivai, phlebotomy vector of atl in the area of study. methods: antibodies antimax were detected by elisa in sera from 42 patients with atl and 63 controls. the extraction of proteins and of dna from l. longipalpis (positive control) and l. neivai was accomplished by the method trizol, following for the detection of proteins by electrophoresis, and genetic expression of max by pcr-rflp (polymerase chain reaction-restriction fragment length polymorphism) with the enzymes hha i and rsa i. results: increased titles of antibodies antimax were observed in atl, compared to controls (p=0,0132). electrophoreses of proteins showed similar fractions for l. longipalpis and l. neivai, and for both a protein fraction with molecular weight similarity to max was observed. the genetic expression of max in l. longipalpis and l. neivai was confirmed by pcr-rflp. conclusions: the description of antibodies antimax in atl patients and controls turned indispensable the research of max in the phlebotomy vector of atl in the area of study. for the first time, it was registered protein and genetic expression of max in l. neivai. the antimax detection in controls confirms the previous exposition to prick of phlebotomies. increased titles of antibodies antimax in atl patients suggest previous and natural exposition to the bite and, consequently, to the protein max, not protecting them of disease and discouraging its employment in vaccination.
Efeito do melado de cana-de-a?úcar no desenvolvimento in vitro de bananeira (Musa spp.) cv. Ma??
Ribeiro, Juliana Martins;Melo, Natoniel Franklin de;Coelho, ?ngela Katiussia Nascimento dos Santos;Pinto, Márcio dos Santos Teixeira;
Revista Ceres , 2012, DOI: 10.1590/S0034-737X2012000300001
Abstract: despite offering a number of advantages as the quality of final product, the technique of in vitro cultivation of plants is still considered expensive due to, among other things, the use of reagents with high purity for the preparation of nutrient media. among the alternatives that can be adopted to reduce the costs associated with the production of seedlings, the replacement of the pa product for those with lower has potential. based on this information, the purpose of this study was to evaluate the effect of molasses of sugar cane on in vitro development of banana cv. ma??. for this purpose, banana plants were inoculated in nutrient media made on the basis of sugarcane molasses, with four different concentrations (brix of 1.5, 3.0, 4.5 and 6.0) and data were compared with those of plants grown on ms medium (control), in a total of five treatments. despite plants have been developed in all treatments, it was observed that for the variables leaf number and fresh biomass, plants cultivated in ms medium (control) had higher performances. however, for the average number of roots, there was no statistical difference between treatments. the sugarcane molasses did not favor the development of banana plants, but can be used for in vitro rooting of these plants.
Hydrogenated fat intake during pregnancy and lactation caused increase in TRAF-6 and reduced AdipoR1 in white adipose tissue, but not in muscle of 21 days old offspring rats
Juliana L de Oliveira, Lila M Oyama, Ana Cláudia L Hachul, Carolina Biz, Eliane B Ribeiro, Claudia M Oller do Nascimento, Luciana P Pisani
Lipids in Health and Disease , 2011, DOI: 10.1186/1476-511x-10-22
Abstract: 21-d-old of T rats had higher serum triacylglycerols, cholesterol, and insulin. The Adipo R1 protein expression was lower in RET and higher in EDL of T group than C. TLR-4 protein content in all studied tissues were similar between groups, the same was verified in TRAF-6 protein expression in soleus and EDL. However, TRAF-6 protein expression in RET was higher in T than C.These results demonstrated that maternal ingestion of hydrogenated vegetable fat rich in TFAs during gestation and lactation decrease in Adipo R1 protein expression and increase in TRAF-6 protein expression in retroperitoneal adipose tissue, but not in skeletal muscle, which could contributed for hyperinsulinemia and dyslipidemia observed in their 21-d-old offspring.Inadequate maternal nutrition during gestation and/or lactation can alter aspects of morphological and physiological development of pups, increasing the predisposition on the adult life to metabolic diseases, like diabetes mellitus and cardiovascular disease [1-3]. Fetal nutrition depends on the concentration of nutrients in maternal bloodstream, placental perfusion, and transfer of these nutrients through placenta [4-6].During gestation, changes in the maternal metabolism occur in order to supply nutrition to the fetus. Lipids play a fundamental role in fetal development. Although lipids transfer through placenta is very limited, changes in dietary fatty acids can lead to implications in fetal and postnatal development [7].Several studies verified that consumption of high amounts of trans fatty acid (TFA) increases blood total cholesterol, VLDL, LDL-cholesterol and triglycerides, and decreases blood HDL-cholesterol, which as consequence increases the chances of development of metabolic syndrome. Also, [8] demonstrated that treatment with TFA has a much greater effect in decreasing adipocyte insulin sensitivity than treatment with saturated fatty acids. The authors explained these results in part by a reduction of plasma membrane fluidi
Waardenburg Syndrome: description of two novel mutations in the PAX3 gene, one of which incompletely penetrant
Pardono, Eliete;Mazzeu, Juliana F.;Lezirovitz, Karina;Auricchio, Maria Teresa B.M.;Iughetti, Paula;Nascimento, Rafaella M.P.;Mingroni-Netto, Regina C.;Otto, Paulo A.;
Genetics and Molecular Biology , 2006, DOI: 10.1590/S1415-47572006000400003
Abstract: we describe two different novel mutations in the pax3 gene, detected in two families with cases of waardenburg syndrome type i (wsi). the missense mutation detected in one family involved a single substitution in exon 2 (c.142 g > t) and was present both in the affected individual and in his clinically normal father. the mutation found in the second family consisted of a deletion of 13 bases, c.764-776del(ttaccctgacatt), in exon 5.
From a Traditional Medicinal Plant to a Rational Drug: Understanding the Clinically Proven Wound Healing Efficacy of Birch Bark Extract
Sandra Ebeling, Katrin Naumann, Simone Pollok, Tina Wardecki, Sabine Vidal-y-Sy, Juliana M. Nascimento, Melanie Boerries, Gudula Schmidt, Johanna M. Brandner, Irmgard Merfort
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0086147
Abstract: Background Birch bark has a long lasting history as a traditional medicinal remedy to accelerate wound healing. Recently, the efficacy of birch bark preparations has also been proven clinically. As active principle pentacyclic triterpenes are generally accepted. Here, we report a comprehensive study on the underlying molecular mechanisms of the wound healing properties of a well-defined birch bark preparation named as TE (triterpene extract) as well as the isolated single triterpenes in human primary keratinocytes and porcine ex-vivo wound healing models. Methodology/Principal Findings We show positive wound healing effects of TE and betulin in scratch assay experiments with primary human keratinocytes and in a porcine ex-vivo wound healing model (WHM). Mechanistical studies elucidate that TE and betulin transiently upregulate pro-inflammatory cytokines, chemokines and cyclooxygenase-2 on gene and protein level. For COX-2 and IL-6 this increase of mRNA is due to an mRNA stabilizing effect of TE and betulin, a process in which p38 MAPK and HuR are involved. TE promotes keratinocyte migration, putatively by increasing the formation of actin filopodia, lamellipodia and stress fibers. Detailed analyses show that the TE components betulin, lupeol and erythrodiol exert this effect even in nanomolar concentrations. Targeting the actin cytoskeleton is dependent on the activation of Rho GTPases. Conclusion/Significance Our results provide insights to understand the molecular mechanism of the clinically proven wound healing effect of birch bark. TE and betulin address the inflammatory phase of wound healing by transient up-regulation of several pro-inflammatory mediators. Further, they enhance migration of keratinocytes, which is essential in the second phase of wound healing. Our results, together with the clinically proven efficacy, identify birch bark as the first medical plant with a high potential to improve wound healing, a field which urgently needs effective remedies.
Trichuris sp. from 1,040 +/- 50-year-old Cervidae coprolites from the archaeological site Furna do Estrago, Pernambuco, Brazil
Sianto, Luciana;Duarte, Ant?nio Nascimento;Chame, Marcia;Magalh?es, Juliana;Souza, M?nica Vieira de;Ferreira, Luiz Fernando;Araújo, Adauto;
Memórias do Instituto Oswaldo Cruz , 2012, DOI: 10.1590/S0074-02762012000200018
Abstract: we present results of the paleoparasitological analysis of cervidae coprolites that were recovered from the archaeological site furna do estrago, pernambuco, brazil. trichuris sp. eggs were recovered from the coprolite samples dated 1,040 ± 50 years before present. this is the first record of trichuris sp. in semiarid cervidae, unexpectedly recorded in archaeological material.
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