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Search Results: 1 - 10 of 569895 matches for " Juan M. Guzmán-Flores "
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Effect of Aerobic Exercise on Protein Expression in Muscle of Obese Mexican Adolescents: A Proteomic and Bioinformatic Analysis  [PDF]
Maciste H. Macías-Cervantes, Juan M. Guzmán-Flores, Katya Vargas-Ortiz, Francisco J. Díaz-Cisneros, Joel Ramírez-Emiliano, Victoriano Pérez-Vázquez
Natural Science (NS) , 2014, DOI: 10.4236/ns.2014.69063

The beneficial effects of exercise have been recognized for many years yet the molecular mechanisms by which exercise show benefits on health are still elusive. A combination of experimental and bioinformatics approaches can be an invaluable tool to increase the cellular understanding of the molecular mechanisms that underlie the aerobic exercise in obese adolescents. Muscle skeletal proteins were separated by 2D-PAGE and changes in protein expression were revealed by ImageMaster 2D Platinum analysis software. Proteins with expression changes after aerobic exercise were identified by comparison with 2D maps from SWISS-2DPAGE and interactions of proteins were analyzed with STRING and DAVID databases. After aerobic exercise, all participants decreased glucose, insulin, total cholesterol, body mass index and waist circumference. Proteomic differential analysis revealed 10 overexpressed proteins after aerobic training, which correspond to carbonic anhydrase III, beta enolase, creatine kinase, ATP synthase beta subunit, aldolase A, glyceraldehyde-3-phosphate dehydrogenase, triosephosphate isomerase, pyruvate kinase, lactate dehydrogenase and adenylate kinase. Exercise increased glycolysis and oxidative phosphorylation pathways, which is likely to be regulated by ubiquitin c protein. In conclusion, our analysis suggested that obese adolescents show changes in their body mass index, waist circumference and serum glucose after aerobic exercise through improved protein expression patterns that aid, especially, glucose metabolism and increase the oxidative phosphorylation. The most likely explanation for the observed pattern is that ubiquitin c protein keeps the glycolytic enzymes bound within skeletal muscle, obtaining a non-degradative role in cell signaling.

Factores de riesgo, asociados a intento de suicidio, comparando factores de alta y baja letalidad
García-Rábago,Horacio; Sahagún-Flores,José E; Ruiz-Gómez,Alfonso; Sánchez-Ure?a,Gustavo M; Tirado-Vargas,Juan C; González-Gámez,Jaime G;
Revista de Salud Pública , 2010, DOI: 10.1590/S0124-00642010000500002
Abstract: objective this study was aimed at identifying the most common risk factors associated with suicide attempts to determine differences between risk factors present in patients regarding their low-lethality and high-fatality suicide attempts. method 106 patients from both sexes who had been hospitalised in a psychiatric unit following their attempts at suicide were interviewed; they were divided into two groups: low-lethality and high-lethality suicide attempt patients. results 58.5 % of the 106 patients were placed in the low-lethality group and 41.5 % in the high-lethality group. the highest rates occurred in the high-lethality group but only two factors had significant statistical difference: "?“living alone" and "?“prior alcohol poisoning". 77.4 % of the sample were aged under 39, 7 % were female and 31 % male. having a family background of alcoholism, previously attempted suicide, generalised anxiety and dysthymia had the highest percentages as risk factors associated with attempted suicide in both groups. the risk factors having the highest percentages were consistent with those reported in the literature. conclusions the fact of living alone and having previously suffered alcohol poisoning had statistically significant differences in this study. no significant differences were found in the rest of the factors studied here.
Evaluation of Morphological Changes of Aeromonas caviae Sch3 Biofilm Formation under Optimal Conditions  [PDF]
Erika Beatriz Angeles-Morales, Ricardo Mondragón-Flores, Juan Pedro Luna-Arias, Cinthia Teresa Enríquez-Nieto, Berenice Parra-Ortega, Graciela Castro-Escarpulli
Advances in Microbiology (AiM) , 2012, DOI: 10.4236/aim.2012.24071

Aeromonas is a Gram-negative bacterium that lives in aquatic habitats. It can be infective in humans. One of its remarkable attributes is the ability of biofilm formation. Many factors are involved in the construction of biofilms as has been described for Pseudomonas, Klebsiella, and Vibrio, among others. The aim of this work was to study the bacterial morphology during the establishment of biofilm through scanning electron microscopy (SEM) and transmission electron microscopy (TEM) with a modified microtiter plate assay and to determine the best conditions for the establishment of Aeromonas caviae Sch3 biolfilm in vitro. We observed several phenotypic changes, including surface appearance, size, presence of extracellular vesicles from 100 to 250 nm in diameter, and flagella. The best conditions for biofilm formation were to grow cultures at 28℃ at pH 6, as determined by the crystal violet assay. This is, to the best of our knowledge, the first study that describes the cell’s biological events involved in the establishment of biofilm formation of Aeromonas caviae Sch3 in vitro.

Development of lntraepithelial Cells in the Porcine Small Intestine
M. A. Vega-López,G. Arenas-Contreras,M. Bailey,S. González-Pozos,C. R. Stokes,M. G. Ortega,R. Mondragón-Flores
Clinical and Developmental Immunology , 2001, DOI: 10.1155/2001/25301
Abstract: The number, phenotype, localisation and development of intraepithelial lymphocytes (IEL) from duodenum (Du) and ileum (Il) were studied by immunohistochemistry (IHC) and light and electron microscopy in unweaned (0–7 weeks old) and six months-old pigs. Developmental changes at birth showed that 38% of the total lymphocytes in the villi were IEL, mainly of the CD2
Durán-Flores,Larissa; Fonthal,Gerardo;
Revista Técnica de la Facultad de Ingeniería Universidad del Zulia , 2003,
Abstract: abstract in this work, the band tail parameters in gap, ge and si, indirect band gap semiconductors, have been studied using the published data. it is established that the optical absorption edge in these materials obeys urbach?s rule. it is also found that the structural disorder in monocrystalline materials is around zero, due to the natural disorder of the crystal lattice. the width of the exponential tail or urbach?s energy is found between 20 and 39mev at 77k and 60-70mev at 300k. also, the steepness parameter s is above 0.55, indicating a strong phonon interaction typical in indirect band gap semiconductors.
Larissa Durán-Flores,Gerardo Fonthal
Revista Técnica de la Facultad de Ingeniería Universidad del Zulia , 2003,
Abstract: Resumen En este trabajo se estudiaron los parámetros de las colas de bandas en GaP, Ge y Si, semiconductores de brecha indirecta, a partir de datos encontrados en la literatura. Se comprobó que el borde de absorción óptico de los materiales estudiados obedece la Regla de Urbach. Se estableció que el desorden estructural en materiales monocristalinos es aproximadamente cero, producto del desorden natural en la red del cristal. El ancho de la cola o energía de Urbach que se determinó en los tres materiales se mantuvo entre 20 y 39 meV para 77K y 60 y 70 meV para 300K. El parámetro de "steepness" obtenido para cada material fue menor de 0.55, describiendo una fuerte interacción fonónica típica en el comportamiento de los semiconductores de brecha indirecta. Abstract In this work, the band tail parameters in GaP, Ge and Si, indirect band gap semiconductors, have been studied using the published data. It is established that the optical absorption edge in these materials obeys Urbach’s Rule. It is also found that the structural disorder in monocrystalline materials is around zero, due to the natural disorder of the crystal lattice. The width of the exponential tail or Urbach’s energy is found between 20 and 39meV at 77K and 60-70meV at 300K. Also, the steepness parameter s is above 0.55, indicating a strong phonon interaction typical in indirect band gap semiconductors.
El impacto biológico de los autoinductores bacterianos
Marcos Flores,Encarnación; Aguilar Gutiérrez,Germán Rubén; Cabrera-Maldonado,Carlos; Guzmán-Flores,José Enrique; Flores-Encarnación,María del Socorro;
Revista de la Sociedad Venezolana de Microbiología , 2011,
Abstract: bacteria, in spite of being unicellular organisms, present great complexity. during a long time they were considered as asocial organisms whose main functions were feeding and reproducing. nevertheless, it has been observed that bacteria are the microorganisms with the greatest capacity for adapting to diverse environments, also responding to multiple stimuli, both nutritional and environmental (ph, oxygen availability, osmolarity, etc.). during the last decades it has been reported that bacteria, both gram negative and gram positive, are capable of communicating among them through specialized cell-communication systems. these systems have been called ?signaling systems? and the signaling molecules which unchain the various cell responses such as biofilm formation, bacterial transformation, luminescence production, antibiotic production, or virulence factor production, among others, have been called ?autoinducers?. this paper presents the most relevant aspects related with gram positive and gram negative bacteria autoinducers, as well as their participation in different biological processes
Hepatitis C virus infection in blood donors from the state of Puebla, Mexico
Francisca Sosa-Jurado, Gerardo Santos-López, Belinda Guzmán-Flores, Julia I Ruiz-Conde, Daniel Meléndez-Mena, Martín T Vargas-Maldonado, Ygnacio Martínez-Laguna, Laura Contreras-Mioni, Verónica Vallejo-Ruiz, Julio Reyes-Leyva
Virology Journal , 2010, DOI: 10.1186/1743-422x-7-18
Abstract: The overall prevalence was 0.84% (515/61553). Distribution by region was: North, 0.86% (54/6270); Southeast, 1.04% (75/7197); Southwest, 0.93% (36/3852); and Central, 0.79% (350/44234). Ninety-six donors were enrolled for detection and genotyping of virus, from which 37 (38.5%) were HCV-RNA positive. Detected subtypes were: 1a (40.5%), 1b (27.0%), mixed 1a/1b (18.9%), undetermined genotype 1 (5.4%), 2a (2.7%), 2b (2.7%), and mixed 1a/2a (2.7%). All recovered donors with S/CO > 39 were HCV-RNA positive (11/11) and presented elevated ALT; in donors with S/CO < 39 HCV-RNA, positivity was of 30.4%; and 70% had normal values of ALT. The main risk factors associated with HCV infection were blood transfusion and surgery.HCV prevalence of donors in Puebla is similar to other Mexican states. The most prevalent genotype is 1, of which subtype 1a is the most frequent.Hepatitis C virus (HCV) infection is an important public health concern. Worldwide, 130 million persons (prevalence of 2-2.2%) are estimated to be infected. The primary diseases associated with HCV are chronic hepatitis, cirrhosis, and cellular hepatocarcinoma [1-3].The actual prevalence of HCV is difficult to assess because serological tests do not discriminate among acute, chronic, or resolved infection, and the analyzed groups in most countries are not representative of the general population, such as blood donors, drug users, or individuals with high-risk sexual practices [3,4].HCV prevalence in Mexico has been analyzed in several studies, reporting an average of 1.4% in the open population and 35% in patients with active hepatitis [5]. Because HCV is relatively variable, it is currently grouped in six genotypes and several subtypes. In Mexico, the prevalence of genotype 1 ranges from 30 to 87.5%, with a predominance of subtypes 1b and 1a. Genotypes 2 and 3 are less frequent and genotypes 4-6 are unusual in Mexican subjects [6].In Mexico, cirrhosis has shown an increasing tendency, rising from 12,058 cases in
Ostrowski type inequalities for interval-valued functions using generalized Hukuhara derivative
Y Chalco-Cano,A Flores-Franuli?,H Román-Flores
Computational and Applied Mathematics , 2012, DOI: 10.1590/s1807-03022012000300002
Abstract: The present paper is devoted to obtaining some Ostrowski type inequalities for interval-valued functions. In this context we use the generalized Hukuhara derivative for interval-valued functions. Also some examples and consequences are presented. Mathematical subject classification: Primary: 26E25; Secondary: 35A23.
Oxidative and Molecular Responses in Capsicum annuum L. after Hydrogen Peroxide, Salicylic Acid and Chitosan Foliar Applications
Laura Mejía-Teniente,Flor de Dalia Durán-Flores,Angela María Chapa-Oliver,Irineo Torres-Pacheco,Andrés Cruz-Hernández,Mario M. González-Chavira,Rosalía V. Ocampo-Velázquez,Ramón G. Guevara-González
International Journal of Molecular Sciences , 2013, DOI: 10.3390/ijms140510178
Abstract: Hydrogen peroxide (H 2O 2) is an important ROS molecule (Reactive oxygen species) that serves as a signal of oxidative stress and activation of signaling cascades as a result of the early response of the plant to biotic stress. This response can also be generated with the application of elicitors, stable molecules that induce the activation of transduction cascades and hormonal pathways, which trigger induced resistance to environmental stress. In this work, we evaluated the endogenous H 2O 2 production caused by salicylic acid (SA), chitosan (QN), and H 2O 2 elicitors in Capsicum annuum L. Hydrogen peroxide production after elicitation, catalase (CAT) and phenylalanine ammonia lyase (PAL) activities, as well as gene expression analysis of cat1, pal, and pathogenesis-related protein 1 ( pr1) were determined. Our results displayed that 6.7 and 10 mM SA concentrations, and, 14 and 18 mM H 2O 2 concentrations, induced an endogenous H 2O 2 and gene expression. QN treatments induced the same responses in lesser proportion than the other two elicitors. Endogenous H 2O 2 production monitored during several days, showed results that could be an indicator for determining application opportunity uses in agriculture for maintaining plant alert systems against a stress.
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