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Search Results: 1 - 10 of 63601 matches for " Juan Antonio Montaraz Crespo "
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Aislamiento y caracterización de cepas de Bordetella bronchiseptica de origen canino
Graciela Molina González,María Eugenia Rosales,Gabriela Bárcenas Morales,Juan Antonio Montaraz Crespo
Veterinaria México , 2006,
Abstract: Se aislaron, identifi caron y caracterizaron cepas de Bordetella bronchiseptica de origen canino. Con ese propósito se tomaron exudados nasales de perros con semiótica de afecciones respiratorias y clínicamente sanos, con ayuda de hisopos impregnados con medio infusión cerebro-corazón. éstos se cultivaron en agar Mac Conkey incubando a 37°C durante 48 h; las colonias presuntivas de Bordetella bronchiseptica se identifi caron por movilidad, tinción de Gram y pruebas bioquímicas. Las cepas se caracterizaron por sus factores de virulencia: hemólisis, hemaglutinación, producción de dermonecrotoxina y presencia de pertactina. Se tomaron 130 exudados en un lapso de ocho meses y se lograron 11 aislamientos. Once animales presentaron afecciones en vías respiratorias y se aislaron seis cepas; ninguna de éstas mostró hemólisis franca, los títulos de hemaglutinación variaron entre las diferentes cepas. Todas las cepas, menos una, mostraron actividad de dermonecrotoxina, y sólo cuatro produjeron pertactina.
Canine Herpesvirus Seroprevalence and Associated Factors in Dogs of Mexico  [PDF]
Edgar Guillermo Valdivia Lara, Jesica Ileana ángeles Solis, Cesar Cuenca Verde, Juan Antonio Montaraz Crespo, Laura Cobos Marín, Juan Carlos Del Río García, Guillermo Valdivia Anda
Open Journal of Veterinary Medicine (OJVM) , 2016, DOI: 10.4236/ojvm.2016.610019
Abstract:
Canine herpesvirus (CHV-1) causes disease associated with high mortality in infect-ed puppies, which represents large financial losses for dog breeders. Since CHV-1 at the time of the study he had not been reported in Mexico, the main objective of this study was to determine the prevalence of antibodies against CHV-1 in canine kennels in the metropolitan area of Mexico City. A commercial enzyme-linked immuno-sorbent assay (ELISA) was used, and the results were compared to those of a viral neutralization test. The ELISA kit uses the complete viral particle as the antigen. The plaque reduction neutralization test was combined with the immunoperoxidase technique because of the low cytopathic effect of CHV-1. Neutralizing antibodies were also detected in 20 randomly selected samples. The prevalence of CHV-1 with ELISA was 87%. The concordance between ELISA and serum neutralization (SN) was 0.1129, the sensitivity of the ELISA against SN was 1.0 (100%), the positive predic-tive value was 0.39 (39%), and the negative predictive value was 1 (100%). These results show that ELISA is useful for monitoring the dog population for CHV-1; a positive test result requires confirmation with an SN test, and a negative ELISA result indicates a high probability of being SN-negative. The only variables that were sta-tistically associated with CHV-1 prevalence were breed and kennel. A statistically significant relationship between the degree of ELISA and SN titer was obtained, with a confidence level of 95%. None of the clinical presentation factors was statistically significant. These results suggest that most of the canine population studied in Mex-ico is in a herpesvirus latency state.
Pathology Isolation and Identification of Canine Herpesvirus (CHV-1) in Mexico  [PDF]
E. Guillermo Valdivia Lara, Blanca Lilia Barrón Romero, Laura Cobos Marín, Jessica Ileana ángeles Solis, Sofía González Gallardo, Cesar Cuenca Verde, Juan Antonio Montaraz Crespo, Guillermo Valdivia Anda
Open Journal of Pathology (OJPathology) , 2016, DOI: 10.4236/ojpathology.2016.63014
Abstract: This work presents the pathology description, isolation and identification of canine herpesvirus (CHV-1) in Mexico, a virus that causes a generalized hemorrhagic infection in puppies from the canidae family. Methods: Isolates were obtained from puppies that died within the first four weeks of life and had lesions consistent with canine herpesvirus. Results: The main gross lesions were petechial and ecchymotic hemorrhages in kidneys, liver and lungs; proliferative interstitial nephritis; multifocal necrosis in liver and kidneys; and encephalitis with intranuclear inclusion bodies. Herpesvirus was confirmed through direct immunofluorescence, electron microscopy and polymerase chain reaction for DNA polymerase and glycoprotein B genes. Discussion: Eight strains were isolated and identified as canine herpesvirus corresponding to three of the working cases with gross and microscopic lesions very similar to those described in the literature; then, isolates were confirmed by PCR gene amplification, positive reactions on immunofluorescence and observations from electron microscopy. This work represents the first report of this disease, including gross and histological lesions, and confirmation by isolation and identification of the canine herpesvirus in Mexico.
Comparación de las constantes fisiológicas sanguíneas de los ratones CD1, heterocigótico et/+ y desnudo et/et diologicas
Francisco Javier Basurto Alcántara,Rosa Luz Mondragón Vargas,Daniel Atilano López,Juan Antonio Montaraz Crespo
Veterinaria México , 2000,
Abstract:
Complicaciones de la utilización del láser
López García,Juan Antonio; Crespo Crespo,Iciar; Aguirreazaldegui García,Lore; Oyarzabal Pérez,Igor;
Archivos Espa?oles de Urología (Ed. impresa) , 2008, DOI: 10.4321/S0004-06142008000900025
Abstract: in this paper we analyze the complications of various applications of laser in urology. the search of a minor aggression trying to diminish complications have lead the development of the use of this energy in the most frequent urologic pathologies. its use in the fragmentation of ureteral stones has modified the therapeutic algorithms. in prostatic diseases we analyze the various options from enucleation (holep) to green laser photovaporization, reviewing the bibliographic references and comparing them with our experience. there are other therapeutic applications that use this energy as a cutting instruments for urethral, ureteral, ureteropyelic junction stenosis, or bladder neck section.
Stenotrophomonas maltophilia Keratitis Related to Therapeutic Contact Lens Misidentified with an Automated Identification System  [PDF]
Daniel Fatela-Cantillo, Javier Crespo-González, Blanca Fatela-Cantillo, Antonio Fernandez-Suarez, Jose Miguel Aguilar Benítez, Juan Antonio Saez Nieto, Remedios González-Amezcua
Open Journal of Clinical Diagnostics (OJCD) , 2015, DOI: 10.4236/ojcd.2015.52012
Abstract: We present a case of keratitis caused by Stenotrophomonas maltophilia in a therapeutic contact lens user with trichiasis and symblepharon. This keratitis was initially diagnosed as caused by Achromobacter xylosoxidans, but the strain was sent for species confirmation and the isolate was finally identified as S. maltophilia by means of 16S rDNA sequencing. The patient rapidly improved on administration of fortified ceftazidime. Physicians should be aware that the definitive identification of the pathogenic agent and prolonged antimicrobial treatment according to culture sensitivities in keratitis are mandatory as treatment success depends greatly on them.
DISE O Y VALIDACIóN DE CONTENIDOS Y MATERIALES PARA LA FORMACIóN ANTE EL ASMA EN CENTROS EDUCATIVOS
Juan Antonio Morales Lozano,Ma José Navarro Monta?o,Manuel Praena Crespo,Grupo GESA
Pixel-Bit , 2009,
Abstract: El objetivo del estudio es mejorar la vida de estudiantes con asma y abordar su atención/tratamiento en centros educativos. Hemos elaborado un Programa de formación dirigido al profesorado de Educación Física en educación secundaria. Presentamos la metodología utilizada en la selección de contenidos, dise o del programa formativo, recursos, validación y difusión. Un análisis comparado de los componentes del Programa sobre aspectos generales, componentes informatizados, aspectos globales y específicos de los materiales. Los resultados permiten valorar positivamente el Programa y sus posibilidades de generalización, por su relevancia en la práctica y su significación en la formación del profesorado de Educación Física.
Public financing and entrepreneurship: behaviour and regional heterogeneity of SMEs
Antonio García-Tabuenca,José Luis Crespo-Espert,Juan R. Cuadrado-Roura
Investigaciones Regionales , 2009,
Abstract: The existence of restrictions for small- and medium-sized enterprises(SMEs) to access long-term credit has led governments to establish institutionalsystems to facilitate such access and reduce the cost of credit, with the conditionthat its feasibility is justified (and assessed) and there are no distortions as regardscompetition. Very few empirical in-depth studies exist regarding this field of academicresearch, and scarce attention has been paid from a regional perspective.Due to the characteristics of the business structures in the different regions, as wellas the existence of agglomeration economies and the regional dispersion of the entrepreneurshiprate, this paper analyses the effects of the productive financing supportmodel, provided by the Government of Spain, through the Instituto de CréditoOficial (ICO) [Official Credit Institute], on the behaviours and performances of thebeneficiary companies. In the last decade, this source of financing has assigned30,000 million euro. The results show the general acceptance of this policy due toits adaptation to the interests of the companies and its contribution to the improvementof the economic-financial efficiency indicators. Regionally, no substantialdifferences have been observed, but the results of this research show a greater contributionto the dynamism of the more progressive regions.
Agudizaciones endodónticas al concluir diferentes variantes técnicas de tratamiento pulporadicular
Guerra Pando,José Antonio; González Crespo,Elienna; Guerra Chico,José Adrián; Afre Socorro,Analina; Madiedo Camargo,Juan Francisco;
Revista de Ciencias M??dicas de Pinar del R?-o , 2009,
Abstract: endodontic acuteness constitutes periapical inflammatory events that are produced during and after concluding the endodontic therapeutic, complicating and increasing the cost of the dental procedures. this research was aimed at determining their onset and intensity at "antonio briones montoto" dentist's teaching clinic from september 2007 to july 2008. the sampling was based on opinions and with 80 cases that were divided into 4 groups of 20 cases applying different treatment schedules. data were collected in tables and graphics and in measures summed up to qualitative variables. a descriptive statistics was used: the two tests, k-proportions and the non-parametric chi-square test to compare the categories as well as the relation and homogeneity of the variables respectively. the total of the cases with acuteness resulted significantly higher with the standardized techniques, no differences in number or the intensity of the acuteness in the treatments in one and two sessions were observed, the best schedule of the treatment was the cervical-apical technique in a session.
El examen oral, una conversación ilustrada
Juan Carlos Crespo
En Blanco y Negro : Revista sobre Docencia Universitaria , 2011,
Abstract: En la experiencia personal, cómo los exámenes orales han ido cambiando de valoración, especialmente al ponerlos en práctica como examen final en los Estudios Generales Letras. Más allá de su significado para la evaluación planificada y rigurosa, se valora la oportunidad para el diálogo inteligente, para conocer aún más a los estudiantes al finalizar el curso y para evaluar sus avances con equidad. Por otro lado, los estudiantes disponen de una alternativa interesante a las pruebas escritas para demostrar el saber argumentar y el exponer con coherencia distinguiendo lo fundamental en temas e ideas, integrando también sus saberes previos y llegando a niveles de análisis más exigentes
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