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Search Results: 1 - 10 of 170862 matches for " José O;Kuniyuki "
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Detec??o do Grapevine virus A e Grapevine virus B por hibridiza??o "dot-blot" com sondas moleculares n?o radioativas
Moreira, Andreia E;Gaspar, José O;Kuniyuki, Hugo;
Fitopatologia Brasileira , 2005, DOI: 10.1590/S0100-41582005000500015
Abstract: grapevine virus a (gva) and grapevine virus b (gvb) are involved in the kober stem grooving and grapevine corky bark diseases, respectively. this work reports the molecular detection of isolates of gva (gva-sp) and gvb (gvb-c-sp and gvb-i-sp) in grapevines (vitis spp.) and tobacco (nicotiana occidentalis) by non-radioactive molecular probes. the digoxigenin-labeled probes were generated by rt-pcr using specific primers to the coat protein genes. total rna was extracted from 45 plants of several grapevine varieties and from 13 plants of tobacco mechanically inoculated with gvb. the rna extracted from infected plants, considered infected by biological indexing, reacted to the cdna probes while there was no hybridization with healthy plants. these results were also confirmed by rt-pcr experiments. the use of the cdna probes hybridization was proved to be efficient in detecting both gva and gvb with high specificity and sensitivity. however, mature leaves and dormant cuttings should be preferably used in diagnostic tests of gvb and gva, respectively.
Caracteriza??o do gene da proteína capsidial do Grapevine virus A em videiras afetadas pela acanaladura do lenho de Kober no Estado de S?o Paulo
Moreira, Andreia E.;Gaspar, José O.;Camargo, Luis E. A.;Kuniyuki, Hugo;
Fitopatologia Brasileira , 2004, DOI: 10.1590/S0100-41582004000200015
Abstract: we report the characterization of the coat protein gene of a brazilian isolate of grapevine virus a (gva-sp). total rna was extracted from leaves and petioles of gva infected plants of the grape (vitis spp.) variety 'kober 5bb' and subjected to rt-pcr using primers designed to amplify a fragment between positions 6409 and 7175 of the gva rna (genbank accession x75433). a fragment of the expected size (767 nt) was obtained which included the coat protein gene coding for 198 amino acids. the gva-sp sequence displayed nucleotide and amino acid similarity of, respectively, 86-92.3% and 94.5-98% with gva isolates from europe, africa and japan (accessions x75433, af441234, af007415, ab039841) and southern brazil (accession af494187). the highest identity, however, was with the african and italian isolates.
Caracteriza o do gene da proteína capsidial do Grapevine virus A em videiras afetadas pela acanaladura do lenho de Kober no Estado de S o Paulo
Moreira Andreia E.,Gaspar José O.,Camargo Luis E. A.,Kuniyuki Hugo
Fitopatologia Brasileira , 2004,
Abstract: O presente trabalho caracteriza o gene codificador da proteína capsidial do isolado do Grapevine virus A (GVA) encontrado no Estado de S o Paulo (GVA-SP). RNA total foi extraído de folhas e pecíolos de plantas de videira (Vitis spp.) da variedade 'Kober 5BB' e submetido a RT-PCR usando oligonucleotídeos desenhados para amplificar um fragmento entre as posi es 6409 e 7175 do RNA do GVA ("GenBank", acesso X75433). Foi obtido um fragmento de tamanho esperado (767 nt) que inclui o gene da proteína capsidial, codificando 198 aminoácidos. A seqüência do GVA-SP apresentou similaridade de nucleotídeos e aminoácidos de, respectivamente, 86-92,3% e 94,5-98% com isolados do GVA da Europa, áfrica e Jap o (Acessos X75433, AF441234, AF007415, AB039841) e da regi o Sul do Brasil (Acesso AF494187), sendo, entretanto, mais similar aos isolados africano e italiano.
Molecular detection of Rupestris stem pitting-associated virus in grapevines in Brazil
Espinha Luciana M.,Gaspar José O.,Kuniyuki H.,Camargo Luis E. A.
Fitopatologia Brasileira , 2003,
Detec??o do Grapevine leafroll-associated virus 5 no Estado de S?o Paulo
Kuniyuki, Hugo;Rezende, Jorge Albeto Marques;Gaspar, José Osmar;Yuki, Valdir Atsushi;
Summa Phytopathologica , 2008, DOI: 10.1590/S0100-54052008000400014
Abstract: grapevine leafroll is a complex disease that can be caused by any one of the nine serologically distinct closteroviruses, referred to as grapevine leafroll-associated viruses 1 to 9 (glrav-1 to glrav-9). so far, it has been known the occurrence of glrav-1, glrav-2, glrav-3 and glrav-6 in brazil. this work reports the identification of glrav-5 in leafroll-infected grape material from the state of s?o paulo, by biotine-elisa. the virus was detected with low incidence in tested grape cultivars, except in 'cardinal', which showed 100% infected. this is the first report of glrav-5 in brazil.
Transmiss?o experimental do vírus do mosaico dourado do feijoeiro por Bemisia argentifolii Bellows & Perring
Yuki, Valdir A.;Louren??o, André L.;Kuniyuki, Hugo;Betti, Juarez A.;
Anais da Sociedade Entomológica do Brasil , 1998, DOI: 10.1590/S0301-80591998000400025
Abstract: since 1991, when the whitefly bemisia argentifolii bellows & perring was first described in s?o paulo, it dispersed rapidly to all the regions of brazil. although the high incidence of geminivirus is correlated with high population of this whitefly, the transmission efficiency of this insect is unknown. to determine whether the b. argentifolii is a vector of bgmv, two experiments were carried out. after an aquisition period of 24 hours on infected beans plants cv. carioca 80, adults of the whitefly were transferred to seedlings of some cultivars, giving a period of inoculation of 24 hours. in the 1st experiment, the percentage of symptomatic plants were: 25.0% for carioca 80, 21.0% for moruna 80, 21.0% for aeté, 75.0% for preto and 26.3% for manteiga. in the 2nd experiment, the percentage of virus transmission were: 63.6% for iac carioca and 60.0% for bt-2. the results showed that b. argentifolii is a vector of bgmv and apparently there are no marked differences in efficiency of transmission between this specie and bemisia tabaci (genn.).
Transmiss?o experimental do Grapevine virus B pela cochonilha Pseudococcus longispinus Targioni-Tozzetti (Hemiptera: Pseudococcidae)
Kuniyuki, Hugo;Gioria, Ricardo;Rezende, Jorge Alberto Marques;Willink, Cristina Granara de;Novo, José Polese Soares;Yuki, Valdir Atsushi;
Summa Phytopathologica , 2006, DOI: 10.1590/S0100-54052006000200009
Abstract: in the state of s?o paulo, brazil, there are two isolates of grapevine virus b (gvb) associated with grapevine corky bark disease (gcb). although serologically similar, they induce distinct reaction on some grape varieties. they are called gvb-c for common isolate and gvb-i for isolate obtained from the variety italia. the objective of this work was to verify the transmission of both gvb isolates from infected to healthy plants by the mealybug pseudococcus longispinus. the transmission of the virus was determined by visual analysis of symptoms, elisa and rt-pcr. in all transmission experiments, grape indicator plants that had been exposed to presumably viruliferous mealybugs reacted in 8-12 months with a typical symptoms of gcb. healthy ln-33 plants, maintained around one gcb-c affected ln-33 plant, highly infested by the mealybug, became infected with incidence of 54.2% after four years. experimental inoculation of healthy ln-33 plants with viruliferous mealybugs resulted in infection rates of 46.2% for gvb-c and 40.0% for gvb-i, after three years. although p. longispinus occurs eventually in s?o paulo state vineyards, preventive control measures for this insect must be taken on areas where healthy clones of scion and rootstock varieties are maintained.
Designing Cancer-Killing Artificial Viruses to Improve Student Understanding of Microbiology
Andy Kuniyuki,Gwen Sharp
Journal of Microbiology & Biology Education , 2011, DOI: 10.1128/jmbe.v12i2.250
Abstract: Our objective was to assess the effectiveness of a “learning by designing” group project used in a lower-division Microbiology course. Students used knowledge gained from the course to design an artificial virus that would kill cancer cells. The assignment required groups to integrate the individual course topics into a unified, complex understanding of the field of microbiology. Throughout the course, students and the instructor collaborated in creating a rubric to evaluate the groups’ final presentations. This paper reports the results of an assessment of the project by comparing the instructor’s and the students’ scores for the presentations. Students’ and the instructor’s scores were correlated; the Pearson coefficient of 0.52 was statistically significant. The results indicate that students gained sufficient knowledge to accurately evaluate proposed designs. Additionally, the overall course grade distribution improved compared to the semester before the project was introduced. Finally, in order to engage students in thinking about their own learning process, they completed a reflection assignment that required them to discuss the changes in their understanding of microbiology over the course of the semester. Our assessment indicates that a design project can serve as an effective and useful learning technique in undergraduate Microbiology courses, though modifications are suggested.
Production of Hydroxylapatite from Biowaste, Chicken Manure by Hydrothermal Process  [PDF]
Sevgihan Yildiz Bircan, Ichiro Naruse, Kozo Matsumoto, Kuniyuki Kitagawa
Journal of Sustainable Bioenergy Systems (JSBS) , 2013, DOI: 10.4236/jsbs.2013.31010

Hydrothermal process has been applied for effective production of Hydrogen from biowastes. In this study hydrothermal process for production of valuable Hydroxylapatitefrom chicken manure containing phosphorus was focused on. Conditions of 400?C and 26 - 27 MPa with addition of 1 mmol Ca(OH)2 were determined as the optimal by using O-phospho-DL-serine as a model compound. Afterwards, the real biowaste containing phosphorous, chicken manure was processed under the same conditions. Formation of a Hydroxylapatite; in the solid residue was confirmed from X-ray diffraction (XRD) patterns, after purification. It was found that 27.9% of P in the chicken manure was converted to Hydroxylapatite. With the use of acetic acid as a chemical purification medium, Hydroxylapatite was obtained.

The Nietzsche’s Reflection on History: Historical Sense and Nihilism  [PDF]
José Ncolao Juli?o
Open Journal of Philosophy (OJPP) , 2018, DOI: 10.4236/ojpp.2018.82007
Abstract: The present article aims to investigate the narratives considerations of Nietzsche’s history, based on his writings in which the critique of historical culture is revealed from its lack of historical sense and his nihilist state, establishing a close relationship between the two themes, making the understanding of the second magnify its critical position.
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