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Search Results: 1 - 10 of 71668 matches for " José Mauro;Taga "
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Radiographic and histological study of perennial bone defect repair in rat calvaria after treatment with blocks of porous bovine organic graft material
Marins, Lucele Vieira;Cestari, Tania Mary;Sottovia, André Dotto;Granjeiro, José Mauro;Taga, Rumio;
Journal of Applied Oral Science , 2004, DOI: 10.1590/S1678-77572004000100012
Abstract: over the last few years, various bone graft materials of bovine origin to be used in oromaxillofacial surgeries have entered the market. in the present study, we determined the capacity of a block organic bone graft material (gen-ox, baumer sa, brazil) prepared from bovine cancellous bone to promote the repair of critical size bone injuries in rat calvaria. a transosseous defect measuring approximately 8mm in diameter was performed with a surgical trephine in the parietal bone of 25 rats. in 15 animals, the defects were filled with a block of graft material measuring 8mm in diameter and soaked in the animal's own blood, and in the other 10 animals the defects were only filled with blood clots. the calvariae of rats receiving the material were collected 1, 3 and 6 months after surgery, and those of animals receiving the blood clots were collected immediately and 6 months after surgery. during surgery, the graft material was found to be of easy handling and to adapt perfectly to the receptor bed after soaking in blood. the results showed that, in most animals treated, the material was slowly resorbed and served as a space filling and maintenance material, favoring angiogenesis, cell migration and adhesion, and bone neoformation from the borders of the lesion. however, a foreign body-type granulomatous reaction, with the presence of numerous giant cells preventing local bone neoformation, was observed in two animals of the 1-month subgroup and in one animal of the 3-month subgroup. these cases were interpreted as resulting from the absence of demineralization and the lack of removal of potential antigen factors during production of the biomaterial. we conclude that, with improvement in the quality control of the material production, block organic bone matrix will become a good alternative for bone defect repair in the oromaxillofacial region due to its high osteoconductive capacity.
Evaluation of the cytocompatibility of mixed bovine bone
Takamori, Esther Rieko;Figueira, Eduardo Aleixo;Taga, Rumio;Sogayar, Mari Cleide;Granjeiro, José Mauro;
Brazilian Dental Journal , 2007, DOI: 10.1590/S0103-64402007000300001
Abstract: treatment of bovine bone with peroxides and chaotropic agents aims to obtain an acellular bone matrix that is able to maintain the collagen-apatite complex and a higher mechanical resistance, a mixed biomaterial hereby named mixed bovine bone (mbb). the purpose of this study was to evaluate the cytocompatibility of mbb and cell-mbb interaction. cell morphology, number of viable cells, ability to reduce methyltetrazolium and to incorporate neutral red upon exposure to different concentrations of the hydrosoluble extract of mbb were assessed in balb-c 3t3 cells according to iso 10993-5 standard. the interaction between cells and mbb surface was evaluated by scanning electron microscopy. the water-soluble mbb extracts were cytotoxic and led to cell death possibly due to its effect on mitochondrial function and membrane permeability. cells plated directly onto the mbb did not survive, although after dialysis and material conditioning in dmem + 10% fcs, the cells adhered and proliferated onto the material. it may be concluded that, in vitro, water-soluble mbb extracts were cytotoxic. nevertheless, mbb cytotoxic effect was reverted by dialysis resulting in a material that is suitable for cell based-therapy in the bioengineering field.
Lack of repair of rat skull critical size defect treated with bovine morphometric protein bound to microgranular bioabsorbable hydroxyapatite
Ferreira, Gabriel Ramalho;Cestari, Tania Mary;Granjeiro, José Mauro;Taga, Rumio;
Brazilian Dental Journal , 2004, DOI: 10.1590/S0103-64402004000300002
Abstract: the ability of a pool of bovine bone morphogenetic proteins bound to synthetic microgranular hydroxyapatite (bmpb-ha) to stimulate bone repair was determined in rat critical size defects. an 8-mm diameter defect was created in the calvaria of 25 rats. in 15 rats, the defects were filled with bmpb-ha homogenized with blood (experimental group), and in 10 rats the defects were filled only with blood clots (control). the calvariae of experimental rats were collected 1, 3 and 6 months after surgery and of the control rats at the end of surgery and 6 months thereafter. the morphometric results obtained in the radiographs showed an absence of new bone formation at 1 and 3 months post-surgery and, histologically, the defects were filled with fibrous connective tissue and numerous foci of a foreign body-type granulomatous reaction around hydroxyapatite agglomerates. at the end of 6 months, the number and size of the granulomatous foci decreased and the area of the defects was reduced by 22% compared to the 0-hour control due to the formation of new bone at their borders, although the mean area was similar to the 6-month control. we conclude that the use of bmpb-ha in the treatment of critical size bone defects of the rat skull leads to the formation of a foreign body-type granulomatous reaction that markedly inhibits new bone formation, suggesting that synthetic microgranular hydroxyapatite does not represent a good carrier for bmp-induced bone formation.
Histologia da associa o de membranas biológicas de origem bovina implantadas no subcutaneo de ratos
Yamatogi Ricardo Seiti,Rahal Sheila Canevese,Granjeiro José Mauro,Taga Rumio
Ciência Rural , 2005,
Abstract: O trabalho teve por objetivo avaliar a capacidade de barreira da membrana de cortical óssea desmineralizada liofilizada bovina, testando sua permeabilidade e integridade, em associa o com a membrana de pericárdio bovino liofilizado. Em 15 ratos Wistar, machos, adultos, implantou-se no subcutaneo da regi o cérvico-torácica dorsal a combina o de duas membranas de cortical óssea desmineralizada tendo no interior a membrana de pericárdio bovino. Os animais (cinco ratos/grupo) foram submetidos à eutanásia aos 15, 30 e 60 dias de pós-operatório. A avalia o microscópica mostrou que, aos 15 dias de pós-cirúrgico, as membranas estavam íntegras, com exce o de pequenas áreas de reabsor o da membrana de cortical óssea junto aos locais referentes aos antigos canais nutritivos. Aos 30 dias, havia apenas restos da membrana de cortical óssea e, aos 60 dias, a sua total ausência. Baseados nos resultados aqui obtidos, é possível concluir que a membrana de cortical óssea bovina é rapidamente absorvida e n o confere prote o à membrana de pericárdio bovino liofilizado.
Análise histológica, radiográfica e do perfil de imunoglobulinas após implanta??o de enxerto de osso esponjoso bovino desmineralizado em bloco em músculo de ratos
Sanada, Jefferson Tomio;Rodrigues, José Gustavo Ribeiro;Canova, Giovana Calicchio;Cestari, Tania Mary;Taga, Eulazio Mikio;Taga, Rumio;Buzalaf, Marília Afonso Rabelo;Granjeiro, José Mauro;
Journal of Applied Oral Science , 2003, DOI: 10.1590/S1678-77572003000300010
Abstract: the aim of this study was to evaluate the biocompatibility of blacks of organic bovine cancellous bone graft material (gen-oxtm, baumer) in ectopic sites. a cylinder of black of graft material measuring 5mm in diameter and 12mm in length was implanted in abdutor muscle of 30 wistar rats. after 3, 7, 14, 21e 28 days, (6 animals/period) were killed, radiographies were taken and the tissues and blood were collected to histological and imunoglobuline g and m profile analysis, respectively. the analysis of the sections revelead an acute inflammatory process at 3 and 7 days, characterized by the presence of neutrophils. absorption of the implanted matrix by mononuclear cells and scarce giant cells and their replacement by fibrous connective tissue rich in vessels and cells, was suggested. at 28 days, in most cases just remnants of the implanted matrix involved by the typical connective tissue of the perimisium were found. there was no radiographic evidence of mineral content or change in the igg and igm profile. on the basis in results described here, it could be concluded that the material is biocompatible and absorbable but, with no signs of osteoindutive capacity was observed.
Tissue response to a membrane of demineralized bovine cortical bone implanted in the subcutaneous tissue of rats
Oliveira, Rodrigo Cardoso de;Menezes, Renato;Cestari, Tania Mary;Taga, Eulázio Mikio;Taga, Rumio;Buzalaf, Marília Afonso Rabelo;Granjeiro, José Mauro;
Brazilian Dental Journal , 2004, DOI: 10.1590/S0103-64402004000100001
Abstract: the treatment of persistent bone defects has encouraged the search for proper techniques or bone substitutes. in dentistry, a common problem in the treatment of periodontal bone defects is the growth of tissues within the lesion, such as the junctional epithelium, which impair regeneration of these tissues. guided tissue regeneration (gtr), based on the separation of the tissues by means of membranes or barriers, was developed in an attempt to improve periodontal regeneration. the aim of this study was to histologically evaluate the tissue response to a membrane of demineralized bovine cortical bone implanted in the subcutaneous tissue of rats. the study periods were 1, 3, 7, 15, 30 and 60 days after implantation. analysis of the histological sections demonstrated a moderate to intense inflammatory response at 1 and 3 days, moderate at 7 and 15 days, and almost absent at 30 and 60 days. resorption of the membrane began 15 days after implantation, and at 60 days only remnants could be detected in some animals. we concluded that the demineralized bovine cortical bone membrane was well tolerated by the tissues and is completely resorbed after 30-60 days by mononuclear cells and multinucleated giant cells, which disappear upon completion of the process.
Effect of bovine bone morphogenetic proteins on radius fracture healing in rabbits
Lima, Alfredo Feio da Maia;Rahal, Sheila Canevese;Volpi, Reinaldo dos Santos;Granjeiro, José Mauro;Taga, Rumio;Cestari, Tania Maria;
Acta Cirurgica Brasileira , 2007, DOI: 10.1590/S0102-86502007000400006
Abstract: purpose: to investigate the effect of bovine bone morphogenetic proteins (bbmps) bound to hydroxyapatite plus collagen in the healing of unstable radius fractures. methods: a transverse fracture was induced at the mid of the diaphysis in both radii on 15 norfolk rabbits with average age of 5.5 months and 3.5kg. a mixture of bbmps bound to thin powdered hydroxyapatite (bbmp-ha) and bovine collagen as agglutinant was applied to the right radius fracture site. the left radius fracture was considered control and no treatment was used. after 30, 60 and 90 days (5 rabbits/period) the rabbits were euthanized and the radii were collected for histological analysis. results: the descriptive histological analysis revealed that repair was similar for both forelimbs. the histomorphometric analysis showed that the mean area of newly formed bone was 867442.16 mm2, 938743.00 mm2 and 779621.06 mm2 for the control forelimbs, and 841118.47 mm2, 788038.76mm2 and 618587.24 mm2 for the treated forelimbs at 30, 60 and 90 days, respectively. thus the newly formed bone area was 12.17% larger in the forelimbs treated with bbmp-ha/collagen than in the control forelimbs (p<0.05, tukey test) in the 60-day period after surgery. in both forelimbs the newly formed bone area increased throughout the experimental period until the complete fracture healing. conclusion: based on the result obtained here we concluded that bbmp-ha/collagen induced a lower but significant improvement in fracture consolidation.
Rat subcutaneous tissue response to macrogranular porous anorganic bovine bone graft
Zambuzzi, Willian Fernando;Oliveira, Rodrigo Cardoso de;Pereira, Felipe Ladeira;Cestari, Tania Mary;Taga, Rumio;Granjeiro, José Mauro;
Brazilian Dental Journal , 2006, DOI: 10.1590/S0103-64402006000400002
Abstract: the ideal bone graft must present biocompatibility, osteoconductive and osteoinductive properties, resistance and plasticity. xenogenic grafts of bovine cancellous bone origin are particularly interesting due to their biologically designed porous structure that enhance both cellular and vascular invasion. the purpose of this study was to evaluate the tissue response induced by bovine macrogranular porous anorganic bone implanted in rat subcutaneous tissue. forty rats were assigned to 2 groups, as follows: the control group received empty collagen capsules and the test group received subcutaneous implants of the test material. samples were collected after 10, 20, 30 and 60 days and processed histologically. histological analysis showed at 10 days a granulomatous inflammatory infiltrate, rich in multinucleated giant cells and free of lymphocytes or plasma cells, similarly to mineralized allograft implanted in rat subcutaneous. in later periods, there was a significant decrease in the inflammatory infiltrate and an increase in fibrosis around graft particles. in conclusion, the test material induced a foreign body-type granuloma with subsequent fibrosis around the graft particles implanted in rat subcutaneous and did not elicit any immune response, thus being considered biocompatible.
Biocompatibility evaluation of a new bioresorbable pin for membrane fixation
Cestari, Tania Mary;Oliveira, Rodrigo Cardoso de;Sanada, Jefferson Tomio;Garlet, Gustavo Pompermaier;Taga, Rumio;Granjeiro, José Mauro;
Brazilian Dental Journal , 2010, DOI: 10.1590/S0103-64402010000600002
Abstract: the aim of this study was to morphometrically analyze the tissue response to a customized pin obtained from devitalized bovine cortical bone (dbcb-pin) implanted in the subcutaneous tissue of rats, as well as to assess its microstructural aspect by scanning electron microscopy (sem). the pins were implanted in the subcutaneous tissue of 20 rats, which were killed at 7, 14, 28 and 60 days (5 rats/period) after implantation. in the subcutaneous tissue, dbcb-pin promoted the formation of a fibrous capsule. at 7 days, capsule showed thickness of 70 ± 3.2 μm with higher density of newly formed capillaries and smaller density of collagen fibers. between 14 and 60 days, more organized fibrous capsule exhibited smaller thickness (53 ± 5.5 μm) with higher density of fibroblasts and collagen fibers. in this period, a small and slow bioresorption of the dbcb-pin by macrophages and rare multinucleated giant cells without tissue damage was observed. the thickness of dbcb-pin resorbed was in mean only of 9.3 μm. during all experimental periods not occurred presence of immune reaction cells as lymphocytes and plasma cells. it was concluded that the pin derived from cortical bovine bone was well tolerated by subcutaneous tissue of rats and slowly resorbed could be an alternative material for membrane fixation in the guided tissue regeneration procedures.
Histologia da associa??o de membranas biológicas de origem bovina implantadas no subcutaneo de ratos
Yamatogi, Ricardo Seiti;Rahal, Sheila Canevese;Granjeiro, José Mauro;Taga, Rumio;Cestari, Tania Mary;Lima, Alfredo Feio da Maia;
Ciência Rural , 2005, DOI: 10.1590/S0103-84782005000400014
Abstract: the aim of this study was to evaluate the barrier capacity of the bovine demineralized lyophilized bone cortical membrane, testing its permeability and its integrity in association with lyophilized bovine pericardium membrane. fifteen male adult wistar rats were used. the combination of two bone cortical membranes with bovine pericardium membrane inside was implanted subcutaneously in the cervical-thoracic area. the animals (five rats per group) were submitted to euthanasia at 15, 30 and 60 days postoperatively. on day 15 postoperative, the microscopic evaluation showed integrity of the membranes, except the absorption of small areas of the bone cortical membrane in the sites of nutritive channels. on day 30, there was a remnant of bone cortical membrane, and on day 60 bone cortical membrane was completely absent. it was possible to conclude that bovine demineralized lyophilized bone cortical membrane is quickly absorbed and does not preserve the lyophilized bovine pericardium membrane.
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