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Search Results: 1 - 10 of 83865 matches for " José Luiz;Matushima "
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Detection of Mycobacterium avium in pet birds
Godoy, Silvia Neri;Sakamoto, Sidnei Miyoshi;Paula, Cátia Dejuste de;Cat?o-Dias, José Luiz;Matushima, Eliana Reiko;
Brazilian Journal of Microbiology , 2009, DOI: 10.1590/S1517-83822009000200011
Abstract: the present study is a report on the presence of mycobacterium avium in four birds of the psittaciform order kept as pets. anatomopathological diagnosis showed lesions suggestive of the agent and presence of alcohol-acid resistant bacilli (aarb) shown by the ziehl-neelsen staining. the identification of mycobacterium avium was performed by means of pra (pcr restriction analysis). dna was directly extracted from tissue of the lesions and blocked in paraffin. the role of this agent in pet bird infection is discussed, as well as its zoonotic potential.
Aspectos morfológicos e citoquímicos dos glóbulos sangüíneos de Caiman crocodilus yacare (Daudin, 1802) (Reptilia, Crocodilia)
MOURA, Weber Leal de;MATUSHIMA, Eliana Reiko;OLIVEIRA, Luiz Waldemar;EGAMI, Mizue Imoto;
Brazilian Journal of Veterinary Research and Animal Science , 1999, DOI: 10.1590/S1413-95961999000100009
Abstract: samples of peripheral blood were collected from 10 healthy caiman crocodilus yacare and stained with leishman, for morphological analysis, or submitted to the following cytochemical methods: pas, sudan black b, o-toluidine and mercury bromophenol blue. seven types of cells were identified: erythrocytes, thrombocytes, heterophils, eosinophils, basophils, lymphocytes and azurofilic monocytes. erythrocytes were elliptical and positive for bromophenol blue. some mitotic figures were noted. spindle-shaped thrombocytes showed large nucleus with striking indentation. the cytoplasm was attenuated at one or both poles into knob-like projections containing glycogen. heterophils revealed round eccentric nuclei and cytoplasm containing glycogen and many polymorphic granules. these granules showed partial positive reaction for sudan and activity for myeloperoxidase. eosinophils showed eccentrically located lenticular-shaped nuclei and numerous round granules stained positive with myeloperoxidase and sudan. basophils revealed a round central nuclei and cytoplasm with few basophilic round granules. lymphocytes appeared as polygonal or irregular cells with thin cytoplasm forming bleb-like protrusions. azurophilic monocytes were round with eccentrically located nuclei and basophilic cytoplasm containing numerous azurophilic granules.
CARACTERISTICAS CITOQUIMICAS MORFOLOGICAS Y ULTRAESTRUCTURALES DE EOSINOFILOS DE Caiman crocodilus yacare (Daudin, 1802) (Reptilia, Crocodilia)
Waldemar Oliveira,Luiz; Leal de Moura,Weber; Reiko Matushima,Eliana; Imoto Egami,Mizue;
Revista chilena de anatomía , 1998, DOI: 10.4067/S0716-98681998000200012
Abstract: summary: samples of peripheral blood from healthy young reptiles were collected from the cervical venous sinus in tubes containing edta 10%. freshly prepared smears were used for the following purpuses: a) staining in rosenfeld solution for the morphological analyses. b) detection of alkaline phosphatase with naphthol asmx-phosphate and myeloperoxidase (ph 7,0 and ph10,0) with o-toluidine h202. the same blood samples with edta were centrifuged for 15' at 2,000rpm and the buffy coat containing leukocytes was devided in two fragments and one of them treated with glutaraldehyde (1h at 4c) and used for alkaline phosphatase reaction and the other 1,25% glutaraldehyde + 1% paraformaldehyde (1h 4c) and used for myeloperoxidase reaction. the eosinophils revealed round, oval or lobed eccentric nuclei with prominent nucleoli. the cytoplasm of eosinophils is dominated by striking eosinophilic granules (large, nearly spherical, closely packed, uniform in size). no alkaline phosphatase activity was observed in the eosinophils. a positive reaction for myeloperoxidase (faint at ph 7,0 an strong at ph 10,0) was visualized as a brown precipitate in the eosinophils. em observation revealed myeloperoxidase activity only in a few granules (at ph 7,0) and in a majority of the granules (at ph 10,0). basic protein reaction product is visible essentially at the periphery of all the citoplasmic granules in the eosinophils.
CARACTERISTICAS CITOQUIMICAS MORFOLOGICAS Y ULTRAESTRUCTURALES DE EOSINOFILOS DE Caiman crocodilus yacare (Daudin, 1802) (Reptilia, Crocodilia) MORPHOLOGICAL AND ELETRONIC CYTOCHEMISTRY OBSERVATIONS IN EOSINOPHIL IN Caiman crocodilus yacare (Daudin, 1802) (Reptilia, Crocodilia)
Luiz Waldemar Oliveira,Weber Leal de Moura,Eliana Reiko Matushima,Mizue Imoto Egami
Revista chilena de anatomía , 1998,
Abstract: RESUMEN: Muestras de sangre periférica de reptiles, jóvenes y sanos, fueron recolectadas del seno venoso cervical, en tubos que contenían EDTA 10%. Inmediatamente los frotis fueron sometidos a los siguientes procedimientos: a) Tinción con solución de Rosenfeld para el análisis morfológico. b)Detección de la fosfatasa alcalina con naftol AS-MX fosfato y mieloperoxidasa (pH 7,0 y pH10,0) con o-toluidina H(2)0(2). Una parte de la sangre con EDTA fue centrifugada por 15' a 2.000rpm y el coágulo conteniendo los leucocitos, fue dividido en dos fragmentos y tratados con: a) glutaraldehído a 2% (1h a 4C) para la reacción de la fosfatasa alcalina; b)glutaraldehído a 1,25% + paraformaldehído al 1% (1h 4C), para la reacción de la mieloperoxidasa. Los eosinófilos revelaron núcleo excéntrico redondeado, oval o lobulado con nucléolo prominente. El citoplasma de los eosinófilos es ocupado por gránulos eosinofílicos evidentes (grandes, aproximadamente esféricos, compactos, uniformes en tama o). No fue observada actividad de la fosfatasa alcalina en eosinófilos. Una reacción positiva débil en pH 7,0 y fuerte en pH 10,0, para mieloperoxidasa fue visualizada como un precipitado marrón, en los eosinófilos. El microscopio electrónico reveló actividad de la mieloperoxidasa solamente en algunos gránulos en pH 7,0 y en la mayoría de los gránulos en pH 10,0. Proteínas básicas produjeron reacción visible, principalmente en la periferia de todos los gránulos citoplasmáticos de los eosinófilos. SUMMARY: Samples of peripheral blood from healthy young reptiles were collected from the cervical venous sinus in tubes containing EDTA 10%. Freshly prepared smears were used for the following purpuses: a) Staining in Rosenfeld solution for the morphological analyses. b) Detection of alkaline phosphatase with naphthol ASMX-phosphate and myeloperoxidase (pH 7,0 and pH10,0) with o-toluidine H(2)0(2). The same blood samples with EDTA were centrifuged for 15' at 2,000rpm and the buffy coat containing leukocytes was devided in two fragments and one of them treated with glutaraldehyde (1h at 4C) and used for alkaline phosphatase reaction and the other 1,25% glutaraldehyde + 1% paraformaldehyde (1h 4C) and used for myeloperoxidase reaction. The eosinophils revealed round, oval or lobed eccentric nuclei with prominent nucleoli. The cytoplasm of eosinophils is dominated by striking eosinophilic granules (large, nearly spherical, closely packed, uniform in size). No alkaline phosphatase activity was observed in the eosinophils. A positive reaction for myeloperoxidase (faint at ph 7,0 an strong at pH
Prevalence of enterobacteria in Bothrops jararaca in S o Paulo State: microbiological survey and antimicrobial resistance standards = Prevalência de enterobactérias em Bothrops jararaca no Estado de S o Paulo: levantamento microbiológico e padr es de resistência antimicrobiana
Henrique Mar?al Bastos,Luiz Fernando Larangeira Lopes,Marco Aurélio Gattamorta,Eliana Reiko Matushima
Acta Scientiarum : Biological Sciences , 2008,
Abstract: There are few microbiological surveys on reptiles in Brazil. The study described here focuses on a species of snake of great medical interest and serves as basis for other studies and comparisons. The samples were collected directly from the colon of healthy adult Jararacas. The material was seeded in MacConkey Agar (Difco ) and XLT4 Agar (Difco ). The isolates were identified through the API 20E Identification System (BioMériux ). The isolates of Salmonella sp. were submitted to serotyping. Finally, the colonies were submitted to the antimicrobial sensitivity test. Several genera of the Enterobacteriaceae family were obtained (Citrobacter, Enterobacter, Escherichia, Klebsiella, Kluyvera, Morganella, Proteus, Providencia and Salmonella), as well as a genus of morphologically similar Gram-negative bacteria (Aeromonas). Salmonella, Citrobacter and Escherichia were the most frequent isolates. Fourteen (14) serotypes of Salmonella were identified; 13 are of the subspecies IIIb and 1 of the subspecies IV. Aeromonas, Enterobacter, Escherichia and Klebsiella presented a higher resistance rate to antibiotics. Kluyvera and Salmonella were the genera most sensitive to the tested drugs. Aminoglycosides presented good antimicrobial action, but chloramphenicol was the only drug to which no isolate presentedresistance. Existem poucos levantamentos microbiológicos em répteis no Brasil. O estudo aqui descrito enfoca uma espécie de serpente de grande interesse médico e serve como base para outros estudos e compara es. As amostras foram colhidas diretamente do cólon de jararacasadultas e saudáveis. O material foi semeado em ágar MacConkey (Difco ) e ágar XLT4 (Difco ). Os isolados foram identificados por meio do Sistema API 20E de Identifica o Bioquímica (Galerias API-BioMériux ). Os isolados de Salmonella sp. foram submetidos àsorotipagem. Por fim, as col nias foram submetidas ao teste de sensibilidade antimicrobiana. Foram obtidos vários gêneros da família Enterobacteriaceae (Citrobacter, Enterobacter, Escherichia, Klebsiella, Kluyvera, Morganella, Proteus, Providencia e Salmonella) e um gênero de bactéria Gram-negativa morfologicamente semelhante (Aeromonas). Salmonella, Citrobacter e Escherichia foram os isolados mais frequentes. 14 sorotipos de Salmonella foram identificados, sendo 13 da subespécie IIIb e 1 da subespécie IV. Aeromonas, Enterobacter, Escherichia e Klebsiella apresentaram maior taxa de resistência aos antibióticos. Kluyvera e Salmonella foram os gêneros mais sensíveis às drogas testadas. Aminoglicosídeos apresentaram boa a o antimicrobiana, mas clo
Interaction of general adaptation syndrome and myocarditis causing ventricular rupture in a black swan (Cygnus atratus): case report
REGO, Alexandre Aparecido Mattos da Silva;MATUSHIMA, Eliana Reiko;FEREIRA, Antonio José Piantino;PINTO, Celso Martins;
Brazilian Journal of Veterinary Research and Animal Science , 1998, DOI: 10.1590/S1413-95961998000500006
Abstract: case report about the interaction of chronic myocarditis and general adaptation syndrome ("stress") causing rupture of left ventricular wall on a young, female specie of cygnus atratus (black swan), that came to die at the country zoo of tabo?o da serra, in s?o paulo, during quarantine, after being taken on a long distance travel, under disfavorable temperature and ventilation conditions.
Isolamento de Salmonella enterica em gambás (Didelphis aurita e Didelphis albiventris) do Estado de S?o Paulo, Brasil
Casagrande, Renata Assis;Lopes, Luiz Fernando Larangeira;Reis, Eliane Moura dos;Rodrigues, Dália dos Prazeres;Matushima, Eliana Reiko;
Ciência Rural , 2011, DOI: 10.1590/S0103-84782011005000016
Abstract: in brazil there is not report of salmonella in opossum, so then, the objective of this study is to determine the isolation frequency of salmonella enterica in opossum in s?o paulo state, brazil. from january 2005 to december 2006, 106 d. aurita and 40 d. albiventris were necropsied and samples from small and large intestine and cloacal swab were collected. these samples were submitted to direct plating in mac conkey agar and parallel suspension in rappaport-vassiliadis and tetrationate broths with posterior streaking in xlt4 agar. the characterization of the isolates was done through biochemical tests and serotyping. salmonella enterica was found in 17.0% (18/106) of the d. aurita; 50% presented the bacteria in the small intestine (si), 88.9% in the large intestine (li) and 66.7% in the cloaca. of the s. enterica were found the subspecies: diarizonae (11.1%), enterica and houtenae (5.5% each); and the serotypes of the s. enterica enterica were newport (83.3%), typhimurium and cerro (5.5% each). in the d. albiventris 17.5% (7/40) were positive; 42.8% in the si, 85.7% in the li and 71.4% in the cloaca. newport (71.4%) was also the most frequent serotype and the second were typhimurium, bareilly and thompson (14.3% each). the presence of salmonella enterica in the intestines of opossums in brazil was proved.
Cutaneous papillomas of green turtles: a morphological, ultra-structural and immunohistochemical study in Brazilian specimens
MATUSHIMA, Eliana Reiko;LONGATTO FILHO, Ademar;DI LORETTO, Celso;KANAMURA, Cristina Takami;SINHORINI, Idércio Luiz;GALLO, Berenice;BAPTISTOLLE, Cecília;
Brazilian Journal of Veterinary Research and Animal Science , 2001, DOI: 10.1590/S1413-95962001000200001
Abstract: eleven juvenile green turtles (chelonia mydas) from atlantic ocean, brazil, with multiple cutaneous papillomatosis were examined. histologically, the papillomas exhibit stromal hyperplasia proliferation and epithelial proliferation. the epithelial cells had nuclear changes suggestive of viral infection and severe nuclear pleomorphism. a large nuclear halo was present in the cases of epithelial proliferation; in these cells, nuclear features were frequently dyscariotic, without inclusion. all fibropapillomas examined were negative for papillomavirus group-specific antigens (bpv) and herpesvirus group-specific antigens (hsv1 / hsv2) by the peroxidase-antiperoxidase technique. electronic microscopy investigation was negative for papillomaviruses and herpes-viruses particles.
Energy Balance in a Patch of the Atlantic Forest in São Paulo City, Brazil  [PDF]
Frederico Luiz Funari, Augusto José Pereira Filho
Journal of Water Resource and Protection (JWARP) , 2014, DOI: 10.4236/jwarp.2014.69076
Abstract:

The aim of this work was to characterize and to bring into relationship of the net radiation with the latent heat flux equivalent to water (mm) in a patch of Atlantic Forest within the metropolitan area of Sao Paulo (RMSP). The estimation of the latent heat flux (LE) was made by the energy balance equation with the Bowen ratio. Measurements of net radiation and psychometrics gradients were made. Soil heat flux (G) was measured by temperature sensors in two depths. Measurements were made at Park of Science and Technology of Sao Paulo University (CIENTEC), Sao Paulo, Brazil, between 2011 and 2013. Results indicated that evapotranspiration varies seasonally and the amount of water evaporated exceeds 3.0 mm·day-1 in spring/summer and in autumn/winter the amount is approximately 1.0 mm·day-1. The evapotranspiration is increased under RMSP urban climate conditions with higher air temperature and lower moisture especially in summer season.

Use of biofeedback (BFB) in the treatment of fecal incontinence after surgical correction of anorectal malformations by posterior sagital anorectoplasty (PSARP)
Martins, José Luiz;Pinus, José;
Sao Paulo Medical Journal , 1997, DOI: 10.1590/S1516-31801997000300005
Abstract: objective: to evaluate biofeedback(bfb)responses to rehabilitation techniques and physical exercises in incontinent or partially continent anorectal malformations patients after posterior sagital anorectoplasty (psarp). design: prospective study. setting: pediatric surgery - department of surgery - unifesp-epm. patients:the authors report on 14 patients with anorectal malformations (4 with partial fecal incontinence after primary psarp; 6 with fecal incontinence after primary psarp; 3 with partial fecal incontinence after secondary psarp; and 1 with fecal incontinence after secondary psarp). all patients were rehabilitated via a bfb program of exercises in order to improve the function of the anal sphincteric muscular complex for a period of 1 -3 years. main outcome measure: clinical and manometric control. results: after bfb, of 4 partially continent patients after primary psarp, 3 became continent; of 6 incontinent patients after primary psarp, 4 became continent; of 3 partially continent patients after secondary psarp, 1 became continent,1 showed no improvement and 1 became incontinent (infection + abscess + fibrosis + important anorectal stenosis). the incontinent patient after secondary psarp showed no improvement. conclusion: the authors concluded that bfb, used at the appropriate time with patient collaboration, is an important complement to the anatomical reconstruction of anorectal malformations in order to achieve good development and contractile functioning of the sphincteric muscular complex.
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