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Search Results: 1 - 10 of 373144 matches for " José B. G.;Freitas "
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Abundance of Conepatus chinga (Carnivora, Mephitidae) and other medium-sized mammals in grasslands of southern Brazil
Kasper, Carlos B.;Bastazini, Vinícius A. G.;Soares, José B. G.;Freitas, Thales R. O. de;
Iheringia. Série Zoologia , 2012, DOI: 10.1590/S0073-47212012000300009
Abstract: between january 2007 and december 2010, the abundance of medium-sized mammals was studied, with special focus on the molina's hog-nosed skunk, conepatus chinga (molina, 1782), at four locations in southern brazil. in this study, transect line methodology was used to obtain data for distance analyses. transects were traveled by car at night, searching with spotlights along the edges of secondary roads in agricultural landscapes. along 1,811 km, we obtained 620 observations of 20 mammal species. the most common species was the exotic european hare, lepus europaeus (pallas, 1778); the highest abundance estimated for south america was observed in one of the study areas, where its density was estimated as 32 individuals/km2. carnivores were the most commonly recorded mammals, represented by 10 species and comprising 51% of all observations. molina's hog-nosed skunk occurred in all study areas, but occurred in sufficient numbers to obtain density estimates in only two of the areas. we estimated 1.4 to 3.8 individuals/km2, in the first density estimate made by the transect method for a member of conepatus in the neotropics. these values are similar to those estimated for north american species of mephitidae. in brazil, c. chinga is apparently more abundant in the pampa biome than in the grasslands of the atlantic forest. for two other carnivores, lycalopex gymnocercus (fisher, 1814) and cerdocyon thous (linnaeus, 1766), we estimated preliminary densities that were similar to those previously cited for different regions.
Parametros hematológicos do roedor fossorial Ctenomys lami (Rodentia, Ctenomidae) no estado do Rio Grande do Sul
Stein, Gisele G.;Lacerda, Luciana A.;Hlavac, Nicole;Stolz, José F.B.;Stein, Ingrid V.;Freitas, Thales R.O.;Carissimi, André S.;
Pesquisa Veterinária Brasileira , 2010, DOI: 10.1590/S0100-736X2010000800011
Abstract: the hematological profile was determined in three populations of ctenomys lami that inhabits three different areas nominated as a and b, affected by cattle production, and c, without human impact, all of them in southern brazil, under the same geologic formation. sixty two individuals were collected. the packed cell volume (pcv) values, hemoglobin (hb) and red blood cell (rbc) count presents statistic significant differences between males and females. pcv and hb values were lower in comparison with other subterranean rodents, which could be related to the food searching behavior or soil characteristic of the species. significant statistic differences were found to for hb, mean corpuscular hemoglobin concentration (mchc) and lymphocytes between animals belonging to a and c areas. the pcv values were higher for animals from areas a and b, with significance only between a and c. some of these alterations may suggest a modification on stress levels of the animals inhabiting areas a and b with anthropic alterations, or maybe just an inherited characteristic. significant statistic differences were found either in mean corpuscular volume (mcv) between a and c areas, and platelets between a and b areas. no kurloff cells were observed in the blood smear from analyzed individuals. the hematological values founded for ctenomys lami are an important information for this species, which can be used for future research and management.
Biochemical and Electrophysiological Characterization of Two Sea Anemone Type 1 Potassium Toxins from a Geographically Distant Population of Bunodosoma caissarum
Diego J. B. Orts,Steve Peigneur,Bruno Madio,Juliana S. Cassoli,Gabriela G. Montandon,Adriano M. C. Pimenta,José E. P. W. Bicudo,José C. Freitas,André J. Zaharenko,Jan Tytgat
Marine Drugs , 2013, DOI: 10.3390/md11030655
Abstract: Sea anemone (Cnidaria, Anthozoa) venom is an important source of bioactive compounds used as tools to study the pharmacology and structure-function of voltage-gated K + channels (K V). These neurotoxins can be divided into four different types, according to their structure and mode of action. In this work, for the first time, two toxins were purified from the venom of Bunodosoma caissarum population from Saint Peter and Saint Paul Archipelago, Brazil. Sequence alignment and phylogenetic analysis reveals that BcsTx1 and BcsTx2 are the newest members of the sea anemone type 1 potassium channel toxins. Their functional characterization was performed by means of a wide electrophysiological screening on 12 different subtypes of K V channels (K V1.1–K V1.6; K V2.1; K V3.1; K V4.2; K V4.3; hERG and Shaker IR). BcsTx1 shows a high affinity for rKv1.2 over rKv1.6, hKv1.3, Shaker IR and rKv1.1, while Bcstx2 potently blocked rKv1.6 over hKv1.3, rKv1.1, Shaker IR and rKv1.2. Furthermore, we also report for the first time a venom composition and biological activity comparison between two geographically distant populations of sea anemones.
Produtos naturais das esponjas marinhas Aaptos sp., Hymeniacidon aff. heliophila, e do nudibranquio Doris aff. verrucosa
Granato, Ana Claudia;Berlinck, Roberto G. S.;Magalh?es, Alviclér;Schefer, Alexandre B.;Ferreira, Antonio G.;Sanctis, Bianca de;Freitas, José Carlos de;Hajdu, Eduardo;Migotto, Alvaro E.;
Química Nova , 2000, DOI: 10.1590/S0100-40422000000500005
Abstract: herein we describe the isolation of homarine and piridiniumbetaine b from the sponge aaptos sp. although homarine has a common occurrence among animals, piridiniumbetaine b was only recently isolated from the marine sponge agelas dispar. the isolation of piridiniumbetaine b from two taxonomically distant marine sponges corroborate previous assumptions that such betaines should be regarded rather as primary metabolites. we have also isolated (9-[5'-(methylthio)-b-d-xylofuranosyl]adenine (xylosyl-mta) from the mantle of a nudibranch identified as doris aff. verrucosa. the occurrence of xylosyl-mta in the mantle of this animal strongly suggests that it is the same nudibranch species described for the mediterranean sea. we have been unable to detect any other compound in the mantle extract of d. aff. verrucosa other than xylosil-mta and sterols. gc-ms analysis of the sterol fraction from the nudibranch and its prey, the sponge hymeniacidon aff. heliophila, revealed the occurrence of the ubiquitous sterols, cholesterol, brassicasterol, cholestanol, 24-methylcholesterol and 24-ethylcholesterol, as the only common metabolites, therefore precluding any assumption concerning the sequestration of secondary metabolites by the nudibranch from h. aff. heliophila.
Caracteriza??o farmacognóstica das folhas e sementes de Albizia lebbeck (L.) Benth. (Fabaceae)
Miranda, Cláudia G.;Arantes, Maria do Carmo B.;Rezende, Maria Helena;Oliveira, Leila M.G.;Freitas, Mara R .F.;Nogueira, Jo?o C. M.;Paula, José R.;Bara, Maria Teresa F.;
Revista Brasileira de Farmacognosia , 2009, DOI: 10.1590/S0102-695X2009000400005
Abstract: albizia lebbeck (l.) benth., is popularly known in brazil as "cora??o-de-negro". it is a species acclimatized in brazil. the seeds of this species are used to treat diarrhea, dysentery and hemorrhoids and the leaves are used for the treatment of ulcers and allergic conjunctivitis. the purpose of this work was to determine the macroscopic and microscopic characteristics of the leaves and seeds of this plant, since these are the parts used popularly. some characters of diagnostical value are pointed out and illustrated by photomicrographs. phytochemistry reactions and determination of water and ash were done on the powder of a. lebbeck. saponins, alkaloids, coumarins were detected in the leaves and seeds; flavonoids were also detected in the leaves. the content of water was 6.78% and 7.91%, respectively, for leaf and seed powder. the values of total ashes and acid insoluble ashes were 9.60% and 0.62% for leaf, and 4.13% and 0.21%, for seed powder. the data obtained are important for the quality control of this medicinal plant. the crude extract of this plant did not show antibacterial activity.
Caracteriza??o fenotípica e molecular de amostras de Burkholderia mallei isoladas na Regi?o Nordeste do Brasil
Silva, Karla P.C.;Mota, Rinaldo A.;Cunha, Arildo P.;Silva, Leonildo B.G.;Leal, Nilma C.;Cavalcante, Yoni V.N.;Teles, José A.A.;Pereira, Maria C.C.;Freitas, Nara S.;
Pesquisa Veterinária Brasileira , 2009, DOI: 10.1590/S0100-736X2009000500015
Abstract: the objective of this paper was to study the molecular performance and phenotypic characterization of burkholderia mallei isolated from horses with clinical and serological diagnosis of glanders, originating from the metropolitan district of recife and zona da mata of pernambuco and alagoas. the isolation and biochemical identification of b. mallei was carried out by microbiological and molecular techniques of pcr-fingerprinting and rapd-pcr. from the eight samples studied, four showed little phenotype variations. in the molecular tests, the samples formed 4 groups of different ribotype profiles and 4 genotype profiles. there was some association of pcr-fingerprinting with rapd-pcr results. it was concluded that the slight biochemical variations were not associated with different molecular profiles. they also indicated that these differences show heterogeneity associated with the origin of the sample, indicating that the infection was caused by clones of different strains and that the polymorphism of dna observed could make it difficult to choose one standard strain for an immune prophylactic treatment of glanders.
Diretrizes da Associa??o Médica Brasileira para o diagnóstico e diagnóstico diferencial do transtorno de ansiedade social
Chagas, Marcos Hortes N.;Nardi, Antonio E.;Manfro, Gisele G.;Hetem, Luiz Alberto B.;Andrada, Nathalia C.;Levitan, Michelle N.;Salum, Giovanni A.;Isolan, Luciano;Ferrari, Maria Cecília Freitas;Crippa, José Alexandre S.;
Revista Brasileira de Psiquiatria , 2010, DOI: 10.1590/S1516-44462010005000029
Abstract: objective: social anxiety disorder is the most common anxiety disorder. the condition has a chronic course usually with no remission and is frequently associated with significant functional and psychosocial impairment. the brazilian medical association, with the project named diretrizes ('guidelines', in english), endeavors to develop diagnostic and treatment protocols for the most common disorders. this work presents the most relevant findings regarding the guidelines of the brazilian medical association concerning the diagnosis and differential diagnosis of social anxiety disorder. method: we used the methodology proposed by the brazilian medical association for the diretrizes project. the search was performed on the online databases medline (pubmed), scopus, web of science, and lilacs, with no time restraints. searchable questions were structured using pico format (acronym for "patient or population"; "intervention, indicator or exposition"; "control or comparison" and; "outcome or ending"). results: we present data regarding the clinical manifestations of social anxiety disorder, impairments and implications related to the condition, differences between the generalized and specific subtypes, and the relationship with depression, drug dependence and abuse, and other anxiety disorders. additionally, the main differential diagnoses are discussed. conclusion: the guidelines are intended to serve as references to the general practitioner and the specialist as well, facilitating the diagnosis of social anxiety disorder.
Claudin-3 Overexpression Increases the Malignant Potential of Colorectal Cancer Cells: Roles of ERK1/2 and PI3K-Akt as Modulators of EGFR signaling
Waldemir F. de Souza, Natalia Fortunato-Miranda, Bruno K. Robbs, Wallace M. de Araujo, Julio C. de-Freitas-Junior, Lilian G. Bastos, Jo?o P. B. Viola, José A. Morgado-Díaz
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0074994
Abstract: The altered expressions of claudin proteins have been reported during the tumorigenesis of colorectal cancer. However, the molecular mechanisms that regulate these events in this cancer type are poorly understood. Here, we report that epidermal growth factor (EGF) increases the expression of claudin-3 in human colorectal adenocarcinoma HT-29 cells. This increase was related to increased cell migration and the formation of anchorage-dependent and anchorage-independent colonies. We further showed that the ERK1/2 and PI3K-Akt pathways were involved in the regulation of these effects because specific pharmacological inhibition blocked these events. Genetic manipulation of claudin-1 and claudin-3 in HT-29 cells showed that the overexpression of claudin-1 resulted in decreased cell migration; however, migration was not altered in cells that overexpressed claudin-3. Furthermore, the overexpression of claudin-3, but not that of claudin-1, increased the tight junction-related paracellular flux of macromolecules. Additionally, an increased formation of anchorage-dependent and anchorage-independent colonies were observed in cells that overexpressed claudin-3, while no such changes were observed when claudin-1 was overexpressed. Finally, claudin-3 silencing alone despite induce increase proliferation, and the formation of anchoragedependent and -independent colonies, it was able to prevent the EGF-induced increased malignant potential. In conclusion, our results show a novel role for claudin-3 overexpression in promoting the malignant potential of colorectal cancer cells, which is potentially regulated by the EGF-activated ERK1/2 and PI3K-Akt pathways.
Growth and Production of Hydroponic Pepper under Salt Stress and Plant Density  [PDF]
Guilherme De Freitas Furtado, Antonio Ramos Cavalcante, Lúcia Helena G. Chaves, José Amilton Santos Júnior, Hans Raj Gheyi
American Journal of Plant Sciences (AJPS) , 2017, DOI: 10.4236/ajps.2017.89151
Abstract: The viability of the use of brackish water in the hydroponic cultivation of vegetables cannot dispense with auxiliary techniques such as water mixing and the use of plant densities. In this sense, the objective of the present work was to analyze the growth and production of sweet pepper, cv. All Big, under hydroponic conditions, under different levels of electrical conductivity of the nutrient solution (1.7, 3.7, 5.7, 7.7, 9.7 and 11.7 dS·m-1) in plants spaced every 0.2 m and 0.3 m. These treatments were distributed in a completely randomized experimental design, analyzed in a 6 × 2 factorial scheme, with five replications. The growth variables were evaluated at 60, 75 and 90 days after sowing and the production variables at the time of harvest; however, the results were submitted to analysis of variance, at a 0.05 probability level. The interaction between the electrical conductivity of the nutrient solution and the density of plants influenced the behavior of the growth and production variables of pepper (All Big). It was verified that the pepper plants had better performance of the biometric variables plant height, stem diameter and number of leaves when cultivated every 30 cm. At the time, in the analysis by plant, the production of pepper fruits was more expressive when spacing of 30 cm was adopted, however, in the tube of six meters, when 20 cm of spacing was used, the number of fruits was on average, 26.38% and 16.40% higher than lower density, in the electrical conductivities 1.7 and 11.7 dS·m-1.
Avalia??o da resistência mecanica de três diferentes pinos de Schanz às for?as de tor??o em montagens do fixador externo monolateral
Mercadante, Marcelo;Kojima, Kodi;Christian, Ralph;Hungria, José Octávio;Hungria Neto, José Soares;Camargo, Alexandre Brás de;Samano, Hélio M.;Santos, Romulo S. F.;Vatavuk, Jan;Mello Junior, Antonio G. de;Bertolani, Andréa Dias;Freitas, Carlos Eduardo B. de;
Acta Ortopédica Brasileira , 2005, DOI: 10.1590/S1413-78522005000400006
Abstract: objective: the objective of this study was to evaluate the mechanical force of three different assemblies of a linear external fixator using 4.5 mm and 5.5 mm schanz screws with a 3.2 mm-diameter root, and another screw with 5.5 mm diameter with a 4,5mm root. material and methods: the linear external fixator was assembled in a 500 mm-long polypropylene tube, with a 45-degree oblique angle cut in the center of the cylinder, with two schanz screws in each segment. eighteen assemblies were studied, divided into three groups of six pieces each. in the group 1, 4.5mm-diameter schanz screws were used, in the group 2, 5.5mm-diameter schanz screws, both with 3,2mm root were used. in the group 3, 5.5mm-diameter schanz screws with a diameter root of 4.5mm were used. the mechanical tests were performed in a mt-100 torsion machine, and the resistance measurements were performed at 4.5o, 9.0o, 13.5o, and 18o of torsion. results: the 4.5mm schanz screws with 3,2 mm root showed an average torsion resistance at 4.5o, 9.0 o, 13.5 o and 18.0 o, respectively of: 12.0 n/mm, 21.0 n/mm, 33.0 n/mm, and 46.0 n/mm. the 5.5mm schanz screw with 3.2 mm root, showed as average resistance: 13.2 n/mm, 25.3 n/mm, 40.0 n/mm, and 51.2 n/mm, respectively. the tests with 5.5 mm schanz screws with 4.5 mm root showed an average resistance of: 15.2 n/mm, 33.5 n/mm, 53.0 n/mm, and 70.0 n/mm. statistical analysis with the variance analysis test and the bonferroni's test showed the absence of statistically significant differences among groups with root diameter of 3.2mm (4.5 mm and 5.5 mm). there was a statistically significant difference between the group with the 5.5mm schanz screws with 4.5 mm root and the other two groups. conclusions: 1- there was no statistically significant difference among assemblies of linear external fixator using 4.5mm and 5.5mm schanz screws with 3.2mm root. 2- the linear external fixator with 5.5mm schanz screws with 4.5mm root, showed a stronger mechanical resistance to the to
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