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Search Results: 1 - 10 of 240831 matches for " José Antonio Apparecido de;Hyppolito "
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Conhecimento da anatomia da orelha de cobaias e ratos e sua aplica??o na pesquisa otológica básica
Albuquerque, Agnes Afrodite Sumarelli;Rossato, Maria;Oliveira, José Antonio Apparecido de;Hyppolito, Miguel Angelo;
Revista Brasileira de Otorrinolaringologia , 2009, DOI: 10.1590/S0034-72992009000100007
Abstract: the use of animal samples is important in otologic research and understanding the anatomy of their ears help make proper use of them in research projects. aim: to study guinea pig's and rat's ears under light microscopy(lm) and scanning electron microscopy(sem) and understand their anatomical advantages in basic otologic research. materials and methods: the temporal bones, tympanic bullas and cochleas from three albino guinea pigs and rats were photographed and analyzed under lm and sem. results: rats aren't as simple to handle as guinea pigs, and often present with otitis media. rats have a fragile junction of the tympanic bulla, two and half turns in the cochlea, and their tympanic membranes do not seal off the entire external auditory canal. guinea pigs have full bullas, their incus and malleus are fused and they have three and half cochlear turns. under sem, guinea pigs and rats have tectori membrane, raissner's membrane and the organ of corti. only guinea pigs have hensen's cells. conclusion: guinea pigs were considered easy to handle for microdissection purposes because of the size and robustness of their temporal bones, and for surgical experiments involving the stapes, the oval window and the tympanic membrane. under sem there are similarities guinea pigs and rats, and both can be used in inner ear studies.
Miringoplastia com a utiliza??o de um novo material biossintético
Oliveira, José Antonio Apparecido de;Hyppolito, Miguel Angelo;Coutinho Netto, Joaquim;Mrué, Fátima;
Revista Brasileira de Otorrinolaringologia , 2003, DOI: 10.1590/S0034-72992003000500010
Abstract: myringosplaty is a surgery that is performed to control infections in middle ear, to reconstruct the sound conducting mechanism for the oval window and protection of the round window. some materials are used to reconstruct the tympanic membrane like autologous temporalis fascia, cartilage-perichondium graft taken from the tragus, cartilage only, dura mater human placental graft. it is important the vascular support to the graft with well-vascularized flap in wide perforations. aim: the main goal of this work is shown the use of a new biomaterial, the natural latex membrane with polylysin. study design: longitudinal cohort. material and method: this material was developed from department of biochemistry from faculdade de medicina de ribeir?o preto universidade de s?o paulo. the natural latex membrane is used stimulating neovessels and organized tecidual growing in different parts and tissues of the human body. this material promotes any allergic reaction and is innocuous to the human tissue. the 238 ears with tympanic membrane perforation resulted from chronic middle ear infection and thek was going through myringoplasty with autologous temporalis fascia and natural latex membrane. the ages were 7 to 76 years. results: the first results show 181 (90,5%) healing tympanic membrane perforation, 96 healing of wide perforation 73 medium perforation and 12 small one. neovessels could be seen in all grafts and remaining tympanic membrane. this is not a habitual funding when the natural latex membrane is not used. conclusion: according to these results we can conclude that the natural latex membrane can be used as a temporary implant in myringoplasty, improving the well vascularized of remaining tympanic membrane.
Miringoplastia com a utiliza o de um novo material biossintético
Oliveira José Antonio Apparecido de,Hyppolito Miguel Angelo,Coutinho Netto Joaquim,Mrué Fátima
Revista Brasileira de Otorrinolaringologia , 2003,
Abstract: A miringoplastia é uma cirurgia com a finalidade de controlar a infec o no ouvido médio, reconstruir o mecanismo de transmiss o sonora para a janela oval e proteger a janela redonda. S o descritos diversos materiais para reconstruir a membrana timpanica, destacando-se a fáscia do músculo temporal, peric ndrio do tragus, cartilagem, dura-máter, tecido placentário, entre outros. OBJETIVO: Este trabalho tem objetivo de demonstrar o efeito de um novo biomaterial, a membrana de látex natural com polilisina, desenvolvida no laboratório de Neuroquímica do Departamento de Bioquímica da Faculdade de Medicina de Ribeir o Preto da Universidade de S o Paulo - USP. FORMA DE ESTUDO: Coorte Longitudinal. MATERIAL E MéTODO: O biomaterial utilizado é estimulante da neovasculariza o e crescimento tecidual organizado em diferentes órg os e tecidos, sendo um material inócuo e n o rejeitado pelo organismo. Foi usada a biomembrana de látex com polilisina como um implante transitório para o fechamento da perfura o da membrana timpanica. A membrana foi colocada na face externa dos bordos da membrana timpanica e a fáscia temporal na face interna da mesma. Foram estudadas 238 orelhas com perfura o de membrana timpanica por seqüela de otite média cr nica, submetidas a miringoplastia com enxerto de fáscia de músculo temporal e a membrana de látex natural, com idades de 7 a 76 anos. Apresentavam uma ou mais miringoplastias anteriores sem sucesso 41 dos casos. RESULTADO: Como ressaltamos preliminarmente, verificamos pega do enxerto em 90,5% das orelhas (181), sendo fechamento de perfura o amplas, 96; médias, 73 e 12 pequenas. Verificamos intensa vasculariza o em 100% dos enxertos, o que n o é habitual quando n o se usa a membrana de látex natural. CONCLUS O: Conclue-se que o biomaterial usado merece nossa aten o quanto ao seu uso como implante transitório em miringoplastias, melhorando o processo de revasculariza o da membrana timpanica remanescente.
Biosilicate? Ototoxicity and Vestibulotoxicity evaluation in guinea-pigs
Massuda, Eduardo Tanaka;Maldonado, Lucas Lisboa;Lima Júnior, Jessé Teixeira de;Peitl, Oscar;Hyppolito, Miguel ?ngelo;Oliveira, José Antonio Apparecido de;
Brazilian Journal of Otorhinolaryngology , 2009, DOI: 10.1590/S1808-86942009000500009
Abstract: changes, destructions and interruptions in middle ear ossicular chain architecture may be caused by infection, trauma, tumors, congenital alterations or prior surgeries. nonetheless, infectious and inflammatory processes, focal or generalized which affect the middle ear are the most prevalent, causing a great demand for ossiculoplasty. biosilicato? is a new material which can be used in the middle ear with the goal of reconstructing the ossicular chain. it is a bioactive type a vitroceramic, in other words, it binds to bone or soft tissue in a matter of a few hours, thanks to the formation of hydroxy-carbonateapatatie in its contact surface when in contact with body fluids. aims: the goal of the present paper is to assess biosilicate ototoxicity and vestibular toxicity in experimental animals, for later use in humans. materials and methods: this a clinical and experimental study in which otoacoustic emissions were performed before and after the placement of biosilicate in the middle ear of experimental animals and a scanning electron microscopy was carried out in the cochlea, saccule, utriculus and macula of the semicircular canals after 30 and 90 days to assess oto and vestibular toxicity. results: there were no signs of oto or vestibular toxicity in any of the groups associated with biosilicate. conclusion: biosilicate is a safe material to be used in ossiculoplasties
The role of hyperbaric oxygen therapy (HOT) as an otoprotection agent against cisplatin ototoxicity
Yassuda, Camila C.;Righetti, Ana Elisa M.;Cury, Maria Cristina L.;Hyppolito, Miguel ?ngelo;Oliveira, José Antonio A. de;Féres, Omar;
Acta Cirurgica Brasileira , 2008, DOI: 10.1590/S0102-86502008000700013
Abstract: purpose: hyperbaric oxygen therapy (hot) consists of intermittent inhalations of 100% oxygen at a pressure higher than 1 atm. it is an important adjuvant therapy in pathological processes like soft tissue infections, radiation injury, gas gangrene, osteomyelitis and decompressive diseases. cisplatin, a potent antineoplastic drug, widely used in cancer therapy is highly ototoxic causing bilateral, irreversible damage to the hearing of high frequency sounds (4-8 khz). objective:this experimental study conducted at the faculty of medicine of ribeirao preto, university of sao paulo aims to evaluate hyperbaric oxygen therapy as an otoprotection agent against drug toxicity. methods: albino guinea pigs were divided into two groups: in group a, 5 animals (10 cochlea) received cisplatin, i. p., 8.0 mg/kg/day during three days and afterwards were submitted to hot; in group b, 3 animals (6cochlea) received cisplatin, i. p. 8.0mg/kg/day during three days. guinea pigs were evaluated by acoustic otoemissions (aoe) and scanning electron microscopy (sem). results: group b animals showed loss of auditory functions as measured by aoe and distorted outer hair cells by sem. in group a, outer hair cells shown by sem images were mostly preserved. conclusion: it is presumed that hyperbaric oxygen therapy has a protector effect against cisplatin ototoxicity.
Ototoxicidade e otoprote??o em orelha interna de cobaias utilizando gentamicina e amicacina: aspectos ultra-estruturais e funcionais
Aquino, Thomaz José Marra de;Oliveira, José Ant?nio Apparecido de;Rossato, Maria;
Revista Brasileira de Otorrinolaringologia , 2008, DOI: 10.1590/S0034-72992008000600006
Abstract: ototoxicity is still a challenge to medicine. the discovery of self-protecting endogenous mechanisms of the outer hair cells associated with their functional and ultra-structural assessment methods has opened new horizons in the understanding and controlling of these mechanisms. aim: this paper aimed at establishing whether or not underdoses of gentamicin could protect the inner ear against the harmful effects of amikacin, based on these protection mechanisms and determine if the otoacoustic emission amplitudes could be associated with the level of hair cell integrity. materials and methods: experimental study. we used 31 guinea pigs. they were injected with saline solution, gentamicin and amikacin, alone and in combinations -intramuscular injections - during 12, 30 and 42 days. the otoacoustic emissions were recorded in the beginning and at the end of the experiment, comparing it with the cochlear integrity study carried out by electron microscopy. results: gentamicin underdoses did not protect the inner ear against amikacin toxicity; the reduction in otoacoustic emissions was strongly associated with an increase in hair cell lesions. conclusion: these findings help understand inner ear otoprotection and ototoxicity. establishing the correlation between the emissions amplitude an cell integrity plays an important role in the follow up of hair cell damage, with possible monitoring of ototoxicity caused by drugs in humans.
Emiss?o otoacústica evocada transitória: instrumento para detec??o precoce de altera??es auditivas em recém-nascidos a termo e pré-termo
Garcia, Cristiane Fregonesi Dutra;Isaac, Myriam de Lima;Oliveira, José Ant?nio Apparecido de;
Revista Brasileira de Otorrinolaringologia , 2002, DOI: 10.1590/S0034-72992002000300009
Abstract: introduction: the early diagnostic and intervention in the hearing alterations are of fundamental importance in the infantile development. the register of the otoacoustic emissions has been enlargement indicated for being a fast exam, easily applied. aim: the objectives of the present study were to value the peripheral hearing function of full term neonate and adequate and small preterm neonate for the gestacional age, by the research of the transient otoacoustic emissions, identifying the prevalence of hearing alterations in this population; to verify the influence from the variable gestacional ages and weight in the moment of the birth, as well as the kinds of treatment, mechanics ventilations, administration of ototoxicity medicines and the permanence in the incubator and to analyze the factors that interfere in the programs of neonatal hearing screening. study design: clinical prospective. material and method: there were appraised 157 children, whose 43 were born full term, 79 preterm adequate to gestacional age and 35 small preterm to gestacional age. it had been observed that premature neonates fail more in the answers from otoacoustic emissions. results: the prevalence of conductive hearing impairment in the population studied was from 29 ears to 1000 and for the sensory-neural hearing impairment from the 16 to 1000. the low weight children in the birth were the most difficult to be appraised. the transient otoacoustic emissions were observed from the beginning of 27 gestacional weeks old. the kinds of treatments used were factors that influenced negatively in the answers of the otoacoustic emissions in premature groups. conclusion: the early diagnostic work of the hearing impairment must be objective of the interdisciplinary team ? neonatologist, pediatrician, ear/nose and throat doctor, audiologist and speech-language pathologist, nurse and relatives ? and must be followed, immediately, by the early interventions programs.
Emiss o otoacústica evocada transitória: instrumento para detec o precoce de altera es auditivas em recém-nascidos a termo e pré-termo
Garcia Cristiane Fregonesi Dutra,Isaac Myriam de Lima,Oliveira José Ant?nio Apparecido de
Revista Brasileira de Otorrinolaringologia , 2002,
Abstract: Introdu o: O diagnóstico e a interven o precoces nas altera es auditivas s o de fundamental importancia no desenvolvimento infantil. O registro das emiss es otoacústicas tem sido largamente indicado, por ser um exame rápido, de fácil aplica o. Objetivo: Os objetivos do presente estudo foram avaliar a fun o auditiva periférica de recém-nascidos a termo e pré-termo adequados e pequenos para a idade gestacional, por meio da pesquisa das emiss es otoacústicas transitórias, identificando a prevalência de altera es auditivas nesta popula o; verificar a influência das variáveis idade gestacional e peso ao nascimento, assim como de tipos de tratamento, ventila o mecanica, administra o de medicamentos ototóxicos e permanência em incubadora e analisar os fatores que interferem nos programas de triagem auditiva neonatal. Forma de estudo: Clínico prospectivo. Material e método: Foram avaliadas 157 crian as, sendo 43 nascidas a termo, 79 pré-termo adequadas à idade gestacional e 35 pré-termo pequenas à idade gestacional. Resultado: Observou-se que recém-nascidos prematuros falham mais nas respostas das emiss es otoacústicas. A prevalência de perda auditiva condutiva na popula o estudada foi de 29 orelhas para 1000 e para perda auditiva neurossensorial de 16 orelhas para 1000. As crian as de peso baixo ao nascimento foram as mais difíceis de serem avaliadas. As emiss es otoacústicas transitórias foram observadas a partir de 27 semanas de idade gestacional. Os tipos de tratamentos utilizados foram fatores que influenciaram negativamente nas respostas das emiss es otoacústicas nos grupos de prematuros. Conclus o: O trabalho de diagnóstico precoce da perda auditiva deve ser objetivo de equipe interdisciplinar -- neonatologista, pediatra, otorrinolaringologista, fonoaudiólogo, enfermeiro e familiares -- e deve ser seguido, imediatamente, por programas de interven o precoce.
Otoprote??o da amifostina aos efeitos ototóxicos da cisplatina: estudo em cobaias albinas por emiss?es otoacústicas produtos de distor??o e microscopia eletr?nica de varredura
Hyppolito, Miguel Angelo;Oliveira, Antonio A. de;Lessa, Ricardo Miranda;Rossato, Maria;
Revista Brasileira de Otorrinolaringologia , 2005, DOI: 10.1590/S0034-72992005000300002
Abstract: cisplatin is an antineoplastic drug for cancer treatment in children and adults. the side effects of cisplatin ototoxicity are significant: irreversible bilateral hearing damage to high frequencies (4 khz - 8 khz). reports recognize some drugs that are associated with cisplatin to obtain an otoprotector effect. the ototoxicity mechanisms of cisplatin are related to injury of hair cell oxidation mechanism, especially of outer hair cells. aim: using otoacoustic emissions distortion products (dpoea) and scanning electron microscopy we intended to verify the action of amifostine, a radioprotective drug that has well known antioxidant characteristics and otoprotector effects to cisplatin injury. study design: experimental. material and methods: we used an experimental guinea pig model. the study was performed as follows: group 1: 6 animals, 12 ears, cisplatin 8.0 mg/kg/day (ip), 3 days. group 2: 6 animals, 12 ears, amifostine 100 mg/kg/day (ip) and after 90 minutes, cisplatin 8.0 mg/kg/day (ip), 3 days and group 3: 3 animals, 6 ears, amifostine 100 mg/kg/day (ip), 3 days. results: dpoea were present before and after treatment in groups 2 and 3. the normal cilium architecture of outer hair cells was supported in all cochlear turns in groups 2 and 3. we concluded that amifostine has a potential otoprotector effect against cisplatin ototoxicity and could be used in clinical trials.
Ototoxicidade da cisplatina e otoprote??o pelo extrato de ginkgo biloba às células ciliadas externas: estudo anat?mico e eletrofisiológico
Hyppolito, Miguel Angelo;Oliveira, José Ant?nio A. de;Rossato, Maria;Holanda, Francisco;
Revista Brasileira de Otorrinolaringologia , 2003, DOI: 10.1590/S0034-72992003000400011
Abstract: cisplatin is an antineoplastic drug for cancer treatment in children and adults. the side effects of cisplatin ototoxycity are important with irreversible auditory and bilateral damage to high frequencies (4khz - 8 khz). reports recognize some drugs that are associated with cisplatin to obtain an otoprotector effect. the ototoxycity mechanisms of cisplatin are related to injury of conduct the hair cell oxidation mechanism, with particular injury to outer hair cells. aim: we intend to studies using otoacoustic emissions - distortion products (dpoea) and scanning microscopy to verify the action of ginkgo biloba (gbe-761) that has well known antoxidizing characteristics, that can function like otoprotector effects. study design: experimental. material and method: we use an experimental guinea pig model. we found dpoea positive before and after treatment in the gbe group (100 mg/ kg/ day - oral) and after 90 minutes cisplatin (8,0 mg/ kg/ day - intraperitoneal - 8 consecutive days). results: the normal cilium architecture of outer hair cells was supported in all cochlear spirals and in the group treated only with cisplatin (8,0 mg/ kg/ day - intraperitoneal - 8 consecutive days), the dpoea was not present and strong injury to cilium of outer hair cells showed cilium disorders upon scanning microscopy. conclusion: we conclude that gbe has a potential otoprotector effect against cisplatin ototoxycity and could be used in clinical trials.
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