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Search Results: 1 - 10 of 22946 matches for " Jorge Timenetsky "
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Avalia??o microbiológica de desinfetantes químicos de uso doméstico
Timenetsky,Jorge;
Revista de Saúde Pública , 1990, DOI: 10.1590/S0034-89101990000100008
Abstract: five disinfectants for household use were advertised on television during 1988 and the first half of 1989. the products were tested by a qualitative (use-dilution with 10 carriers, a conventional and simplified, adapted method) and a qualitative, adapted method with a view to evaluating their antimicrobial activity. the active compounds of the products, according to their respective labels were: 1 - parachlorophenol (0-benzil) 0.1%; 2 - eter 2.4.4' chloro (iii) 2' hydroxiphenylic 0.1%; 3 - n - alkyl dimethylbenzyl ammonium chlorides, n - alkyl dimethylethybenzyl ammonium 50% - 1.6%; 4 - formaldehyde 37% (0.3% solution); 5 - no information. the microorganisms used were: staphylococcus aureus atcc 6538, pseudomonas aeruginosa atcc 15442 and salmonella choleraesuis atcc 10708. in the qualitative method the pseudomonas strain was recovered from disinfectants 1, 2, and 3 and the salmonella strain from disinfectants 2 and 3. all disinfectants showed germicidal effect 5.0 (99.999%) of reduction) in 15 seconds against all strains. disinfectant 3 was contamined with enterobacter sp to the order of 104 cells/ml. this contaminant was sensitive against disinfectants 1, 4 and 5, in the qualitative method and had a relative resistance to disinfectant 2 in the quantitative method.
Rea??o de coaglutina??o estafilocócica na identifica??o de micoplasmas
Timenetsky, Jorge;Curcio, Melissa;
Revista do Instituto de Medicina Tropical de S?o Paulo , 1993, DOI: 10.1590/S0036-46651993000600012
Abstract: staphylococcal coagglutination was used as method for a rapid identification of mycoplasmas that could be performed by non specialized laboratories. suspensions of staphylococcus aureus (cowan i) sensitized with rabbit antibodies against nctc mycoplasma strains have identified these microorganisms and the strains isolated from humans, cell cultures rats and mice in concentrated suspensions from cultures of 4.0 ml. fourty eight strains of m.pulmonis, 6 of m. arthritidis, 8 of m.arginini, 3 of m.orale, 15 of a.laidlawii, 8 of m.hominis and 3 of m.pneumomae were identified by staphylococcal coagglutination and confirmed by growth inhibition test. optimal parameters of coagglutination were established and the stability of the conjugates were preserved for 90 days when added with acetyl cistein. the reaction was visualized without optical resources. the sera were previously absorved with heterologous nctc strains and with the pellet of the steril broth.
Coeficiente fenólico na avalia??o microbiológica de desinfetantes de uso hospitalar e doméstico
Timenetsky,Jorge; Alterthum,Flávio;
Revista de Saúde Pública , 1989, DOI: 10.1590/S0034-89101989000200011
Abstract: the phenolic coefficient of 24 disinfectants (six for hospital and the remainder for household use) commercialized in s. paulo were verified. the active compounds found were phenol, quaternary ammonium, formaldehyde, ethanol and chlorine, some of then in association. the microorganisms used were staphylococcus aureus atcc 6538, pseudomonas aeruginosa atcc 15442 and salmonella choleraesuis atcc 10708. the values of the phenolic coefficient ranged from 58.3 to 0.1. the hospital disinfectants showed values greater than those of the disinfectants for household use, though these differences do not necessarily indicate the quality of the respective products. conversely the microbiological method adopted showed that some products for household use had low or inexistent antibacterial activity because the phenolic coefficient could not be determined for the dilutions used for this evaluation.
Immunoblot profiles of sera from laboratory rats naturally infected with Mycoplasma pulmonis and technicians exposed to infected animal facilities
Delgado, Márcio Oliveira;Timenetsky, Jorge;
Brazilian Journal of Microbiology , 2001, DOI: 10.1590/S1517-83822001000400009
Abstract: mycoplasma pulmonis have been isolated in about 105 cfu/ml from tracheal aspirates of rats from conventional animal facilities in s?o paulo. the mycoplasma transmission by aerosol may happen from an infected rat to a healthy one at distances up to 120 cm. this condition also favors the technicians contamination. as this infection is unknown in humans, in this study the immunoblot profiles to m. pulmonis of sera from rats were compared to those presented by animal facility technicians. about 32 proteins from 11 to 230 kda (kilodaltons) were recognized by the sera from rats naturally infected with m. pulmonis. sera from technicians responsible for the cleaning and sanitation of cages of infected animals for more than seven years recognized about 10 proteins of this bacteria. sera from individuals with shorter working time or that had never been exposed to such environment recognized few proteins. proteins about 117 and 95 kda were recognized by human and rat sera and by the negative controls. although a positive human serum against m. pulmonis is unknown, this study established a temporary profile of protein recognition of human serum against such mycoplasma.
Avalia??o da contamina??o bacteriana em desinfetantes de uso domiciliar
Miyagi,Fumie; Timenetsky,Jorge; Alterthum,Flávio;
Revista de Saúde Pública , 2000, DOI: 10.1590/S0034-89102000000500003
Abstract: objective: to evaluate disinfectants for domestic use for the presence of bacteria, identify them, and determine their tolerance level to benzalkonium chloride. methods: fifty-two samples of commercially available disinfectants for domestic use were acquired at random in the metropolitan area of s?o paulo, brazil, and analyzed to detect the presence of bacterial contaminants. the isolated organisms were identified and their tolerance level to benzalkonium chloride was determined by broth macrodilution method. results: sixteen (30.77%) of fifty-two disinfectants sampled were contaminated by gram-negative bacteria, with counts varying between 104 and 106 ufc/ml. alcaligenes xylosoxidans, burkholderia cepacia and serratia marcescens were the predominant organisms found. the minimum inhibitory concentration (mic: mg/ml) of benzalkonium chloride for these bacteria were 2.48, 1.23 and 0.30 to s. marcescens, a. xylosoxidans and b. cepacia, respectively. conclusions: the disinfectant formulation containing quaternary ammonium compounds (qacs) may be exposed to contamination by gram-negative bacteria. the mics of benzalkonium chloride against the isolated bacteria were low, indicating that the bacteria grown in culture media without qacs lost their tolerance to this biocide.
Avalia o da contamina o bacteriana em desinfetantes de uso domiciliar
Miyagi Fumie,Timenetsky Jorge,Alterthum Flávio
Revista de Saúde Pública , 2000,
Abstract: OBJETIVO: Avaliar desinfetantes de uso domiciliar, identificando a presen a de bactérias contaminantes, e conhecer o nível de tolerancia dessas bactérias ao cloreto de benzalc nio. MéTODOS: Foram adquiridas aleatoriamente no comércio da regi o metropolitana de S o Paulo, SP, Brasil, 52 amostras de desinfetantes de uso domiciliar para análise quanto à presen a de bactérias contaminantes. O nível de tolerancia dessas bactérias ao cloreto de benzalc nio foi determinado pelo método da macrodilui o em caldo. RESULTADOS: De 52 amostras, 16 (30,77%) estavam contaminadas por bactérias Gram negativas, com contagens variando entre 10(4) e 10(6) UFC/ml. Esses contaminantes foram identificados como Alcaligenes xylosoxidans, Burkholderia cepacia e Serratia marcescens. As Concentra es Inibitórias Mínimas (CIM: mg/ml) do cloreto de benzalc nio para S. marcescens, A. xylosoxidans e B. cepacia foram: 2,48, 1,23 e 0,30, respectivamente. CONCLUS ES Os desinfetantes de uso domiciliar à base de compostos de am nio quaternário s o passíveis de contamina o por bactérias. As CIM do cloreto de benzalc nio para as bactérias contaminantes estavam abaixo das concentra es do princípio ativo presente nos desinfetantes, indicando que a tolerancia ao biocida n o é estável, podendo ser perdida com o cultivo das bactérias em meios de cultura sem o biocida.
Immunoblot profiles of sera from laboratory rats naturally infected with Mycoplasma pulmonis and technicians exposed to infected animal facilities
Delgado Márcio Oliveira,Timenetsky Jorge
Brazilian Journal of Microbiology , 2001,
Abstract: Mycoplasma pulmonis have been isolated in about 10(5) CFU/mL from tracheal aspirates of rats from conventional animal facilities in S o Paulo. The mycoplasma transmission by aerosol may happen from an infected rat to a healthy one at distances up to 120 cm. This condition also favors the technicians contamination. As this infection is unknown in humans, in this study the immunoblot profiles to M. pulmonis of sera from rats were compared to those presented by animal facility technicians. About 32 proteins from 11 to 230 kDa (kilodaltons) were recognized by the sera from rats naturally infected with M. pulmonis. Sera from technicians responsible for the cleaning and sanitation of cages of infected animals for more than seven years recognized about 10 proteins of this bacteria. Sera from individuals with shorter working time or that had never been exposed to such environment recognized few proteins. Proteins about 117 and 95 kDa were recognized by human and rat sera and by the negative controls. Although a positive human serum against M. pulmonis is unknown, this study established a temporary profile of protein recognition of human serum against such mycoplasma.
Sensitivity of rabbit fibrochondrocytes to mycoplasmas
Nascimento, Carlos Manuel de Oliveira;Figueiredo, Cristina Adelaide;Timenetsky, Jorge;
Brazilian Journal of Microbiology , 2002, DOI: 10.1590/S1517-83822002000300011
Abstract: primary cell culture from rabbit meniscus (fibrochondrocytes-fcrc) was infected for 24 hours with different inocula (102 to 107 colony forming units-cfu) of mycoplasma hominis pg-21, m. pneumoniae fh and 1428 or m. arthritidis pg-6. the severity of the different obtained cytophatic effects-cpe was inoculum, mycoplasma species and strain dependant. these bacteria were recovered from all infected fcrc and the sp4 medium for mycoplasmas also caused toxic effect on the fcrc. it was concluded that rabbit fibrochondrocytes were sensitive to mycoplasma infection, as well as to the sp4 mycoplasma medium.
Avalia??o de desinfetantes químicos de uso doméstico contra Vibrio cholerae EL TOR (amostra n?o toxigênica)
Timenetsky,Jorge; Yanaguita,Roberto Mitio; Silva,Luzinete Alves;
Revista de Saúde Pública , 1992, DOI: 10.1590/S0034-89101992000500005
Abstract: the methodology of microbiological evaluation of disinfectants is permanently being questioned because the laboratorial protocols do not correspond to the real conditions under which these products are used. in 1985 the use-dilution method of aoac was adopted in brazil for microbiological qualification of chemical disinfectants for commercial purposes. domestic disinfectants are tested in this way against salmonella choleraesuis and staphylococcus aureus atcc strains, was chosen for this evaluation vibrio cholerae in view of its current importance in brazil, in terms of public health associated with the study of the disinfectant's antimicrobial activities. nineteen disinfectant products for domestic use for available to the public were evaluated microbiologically by means of simplified use-dilution test with 10 carriers. the active compounds of the products included formaldeyde, phenols, cresols, quaternary ammonium compouds, chlorine and ethanol. seven were mixtures of these. according to the recommendations for their use, sixteen products should be used undiluted. under these conditions, 9 disinfectants were vibriocides and 7 did not demonstrate this antibacterial activity. four products in dilutions not clearly specificated were also ineffective. the vibriocide products which must used without dilution were tested again, diluted at 1:2. these solutions did not inactivate v. cholerae showing that, microbiologically, their active compounds are used in limited concentrations. commercial alcohol (95.5°gl) at 1:3, chlorine 2.8% água sanitária at 1:200 and lysoform at 1:20 came up to the standards required by the test.
Avalia o de desinfetantes químicos de uso doméstico contra Vibrio cholerae EL TOR (amostra n o toxigênica)
Timenetsky Jorge,Yanaguita Roberto Mitio,Silva Luzinete Alves
Revista de Saúde Pública , 1992,
Abstract: As metodologias de avalia o microbiológica de desinfetantes s o permanentemente questionadas porque os protocolos laboratoriais n o representam as condi es reais de uso desses produtos. Em 1985, adotou-se no Brasil, a metodologia da Dilui o-Uso da AOAC, para a qualifica o microbiológica de desinfetantes químicos, para fins comerciais. Desta maneira, os desinfetantes domésticos s o testados contra amostras padr es de Salmonella choleraesuis e Staphylococcus aureus. Pesquisou-se o emprego de Vibrio cholerae devido a sua atual importancia, no Brasil, em termos de Saúde Pública, associada ao estudo da atividade antimicrobiana de desinfetantes. Dezenove produtos desinfetantes de uso doméstico encontrados no comércio foram microbiologicamente avaliados. A metodologia foi a Dilui o-Uso com 10 carreadores. Os compostos ativos dos produtos incluíam: formaldeído, fenóis, cresóis, am nio quaternário, cloro e etanol, sendo que sete, eram de composi o associada. Conforme as recomenda es de uso, dezesseis produtos, devem ser utilizados sem dilui o. Nestas condi es, 9 desinfetantes foram vibriocidas e sete n o revelaram tal atividade antibacteriana. Quatro produtos em dilui es n o esclarecedoras para a desinfec o também mostraram-se ineficazes. Os produtos vibriocidas que devem ser utilizados sem dilui o, foram reavaliados diluídos ao dobro. Estas solu es n o inativaram V.cholerae, demonstrando microbiologicamente que os seus compostos ativos est o em concentra es limítrofes. O álcool comercial (95,5degrees GL) a 1:3, a "água sanitária" (2,8% de cloro ativo) a 1:200, creolina a 1:10 e o "Lysoform" a 1:20 atingiram os padr es do teste.
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