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Search Results: 1 - 10 of 37418 matches for " Jorge Henrique;Pinto "
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An Efficient Method for Genomic DNA Extraction from Different Molluscs Species
Jorge C. Pereira,Raquel Chaves,Estela Bastos,Alexandra Leit?o,Henrique Guedes-Pinto
International Journal of Molecular Sciences , 2011, DOI: 10.3390/ijms12118086
Abstract: The selection of a DNA extraction method is a critical step when subsequent analysis depends on the DNA quality and quantity. Unlike mammals, for which several capable DNA extraction methods have been developed, for molluscs the availability of optimized genomic DNA extraction protocols is clearly insufficient. Several aspects such as animal physiology, the type (e.g., adductor muscle or gills) or quantity of tissue, can explain the lack of efficiency (quality and yield) in molluscs genomic DNA extraction procedure. In an attempt to overcome these aspects, this work describes an efficient method for molluscs genomic DNA extraction that was tested in several species from different orders: Veneridae, Ostreidae, Anomiidae, Cardiidae (Bivalvia) and Muricidae (Gastropoda) , with different weight sample tissues. The isolated DNA was of high molecular weight with high yield and purity, even with reduced quantities of tissue. Moreover, the genomic DNA isolated, demonstrated to be suitable for several downstream molecular techniques, such as PCR sequencing among others.
Produ??o de mudas de hortel?-japonesa em fun??o da idade e de diferentes tipos de estaca
Chagas, Jorge Henrique;Pereira Pinto, José Eduardo Brasil;Bertolucci, Suzan Kelly Vilela;Nalon, Felipe Hébia;
Ciência Rural , 2008, DOI: 10.1590/S0103-84782008000800011
Abstract: the objective of this research was to verify the influence of different age and types of cuttings in the production of scion of mentha arvensis l. four different types of cuttings were evaluated: apical and medium cuttings (from aerial part); tip and medium cuttings (from rhizome part). the different types of cuttings were evaluated at 25 and 40 days of age. the cuttings used had 4-5cm long and covered with plantmax? substrate to a depth of 2/3. two rooting periods (25 and 40 days) from the different types of cuttings were evaluated: height, shoots number, aerial and roots dry biomass and percentage of mortality. the cuttings after the rooting period were transplanted to bed and pots. the mortality and the aerial part, roots and rhizome dry biomass were determined 30 days after being cultivated. the results showed that apical cuttings from aerial part at 25 and 40 days after being planted in trays had higher rooting, aerial part development and low mortality. at 25 days the aerial part developed and the scion was ready to be transplanted to field. thirty days after transplanted apical cuttings from aerial part showed a higher growth, except for rhizome dry biomass.
Gastroschisis: preterm or term delivery?
Soares, Henrique;Silva, Ana;Rocha, Gustavo;Pissarra, Susana;Correia-Pinto, Jorge;Guimar?es, Hercília;
Clinics , 2010, DOI: 10.1590/S1807-59322010000200004
Abstract: aim: the main objective of this study was to evaluate the association between prematurity and the time to achieve full enteral feeding in newborns with gastroschisis. the second objective was to analyze the associations between length of hospital stay and time to achieve full enteral feeding with mode of delivery, birth weight and surgical procedure. methods: the medical records of newborns with gastroschisis treated between 1997 and 2007 were reviewed. two groups were considered: those delivered before 37 weeks (group a) and those delivered after 37 weeks (group b). the variables of gestational age, mode of delivery, birth weight, time to achieve full enteral feeding, length of hospital stay and surgical approach were analyzed and compared between groups. results: forty-one patients were studied. in group a, there were 14 patients with a mean birth weight (bw) of 2300 g (range=1680-3000) and a mean gestational age (ga) of 36 weeks (range=34-36). in group b, there were 24 patients with a mean bw of 2700 g (range=1500-3550) and a mean ga of 38 weeks (range=37-39). the mean time to achieve full enteral feeding was 30.1±6.7 days in group a and 17.0±2.5 days in group b (p=0.09) with an or of 0.82 and a 95% ci of 0.20-3.23 after adjustment for sepsis and bw. no statistical difference was found between low bw (<2500 g), mode of delivery and number of days to achieve full enteral feeding (p=0.34 and p=0.13, respectively). patients with bw over 2500 g had fewer days in the hospital (22.9±3.1 vs. 35.7±5.7 days; p=0.06). conclusion: the results of this study do not support the idea of anticipating the delivery of fetuses with gastroschisis in order to achieve full enteral feeding earlier.
Crescimento vegetativo e produ??o de óleo essencial de hortel??pimenta cultivada sob malhas
Costa, Andressa Giovannini;Chagas, Jorge Henrique;Pinto, José Eduardo Brasil Pereira;Bertolucci, Suzan Kelly Vilela;
Pesquisa Agropecuária Brasileira , 2012, DOI: 10.1590/S0100-204X2012000400009
Abstract: the objective of this work was to evaluate the growth and the content, yield, and chemical composition of the essential oil of peppermint (mentha piperita) grown under different nets in lavras, mg, brazil. peppermint plants were grown in five environments: full sun, black net, aluminet net, blue net, and red net, all with 50% of irradiance. the experimental design was a completely randomized block with four replicates and five plants per plot. the evaluated parameters were: vegetative growth, content, yield, and chemical analysis of the essential oil. peppermint plants grown under full sun and black and red nets produced higher dry leaf biomass and higher essential oil content and yield. however, in full sun, the essential oil of the plants had higher contents of menthol and, under black and blue nets, of menthophurane. therefore, it is possible to manipulate plant growth and yield and chemical composition of the essential oil of peppermint with cultivation under nets or full sun.
Sistemas de manejo da palhada influenciam acúmulo de biomassa e produtividade da cana-de-a úcar (var. RB855453) = Straw management systems influence biomass accumulation and yield of sugarcane crop (var. RB855453)
Luiz Henrique Franco de Campos,Saul Jorge Pinto de Carvalho,Pedro Jacob Christoffoleti,Caio Fortes
Acta Scientiarum : Agronomy , 2010,
Abstract: A manuten o da palha de cana-de-a úcar sobre a superfície do solo, após colheita sem queima, pode prejudicar a brota o, o perfilhamento e a produtividade da cultura, principalmente em regi es mais frias, além de contribuir para maiores níveis de infesta o por cigarrinhas (Mahanarva fimbriolata). Assim sendo, este trabalho foi desenvolvido com o objetivo de avaliar o perfilhamento, o acúmulo de biomassa fresca e a produtividade da soqueira de cana-dea úcar (var. RB855453), bem como a taxa de infesta o por cigarrinhas, quando a cultura foi submetida a três sistemas de manejo da palhada: (i.) palha em área total; (ii.) linha descoberta; e (iii.) aleiramento. Em virtude do rigor experimental do teste de Tukey e da representatividade de cada parcela (2.700 m2), concluiu-se que a manuten o da palha de cana-de-a úcar em área total sobre o solo, após colheita sem queima, prejudicou o desenvolvimento da soqueira, que apresentou menor produtividade e biomassa de perfilhos. O aleiramento da palha, embora n o tenha contribuído para a variável número de perfilhos, proporcionou maior biomassa de colmos e produtividade, sem diferen a para o manejo de linhas descobertas. Identificou-se menor infesta o por cigarrinhas no tratamento com linhas descobertas, contudo o nível de controle n o foi atingido, de forma que os danos entomológicos n o devem ter contribuído para o menor rendimento da cultura quando sob palhada em área total. Maintaining sugarcane straw over the soil surface after harvest without burning may damage sugarcane sprouting, tillering and crop yield, mainly in cold climate sites, in addition to contributing to higher levels of spittlebug (Mahanarva fimbriolata) infestations. Therefore, this work was developed with the objective of evaluating the tillering, fresh biomass accumulation and yield of sugarcane ratoon (var. RB855453), as well as the infestation level of M. fimbriolata, when the crop was submitted to three different strawmanagement systems: (i.) straw in total area; (ii.) uncovered rows and (iii.) accumulating the straw of three rows in one interrow. As a result of experimental rigor provided by the Tukey test and the size and representativeness of each plot (2700 m2), it was possible to conclude that keeping sugarcane straw over the soil in total area, after harvest without burning, reduced ratoon development, presenting lower yield and tiller biomass. Straw accumulation of three rows in one interrow did not contribute to the number of tillers; however, it promoted higher biomass of tillers and consequently higher yield, without difference
O ensino de línguas baseado em tarefas e o foco na forma: contributos para uma didática do PL2 em cabo verde
Jorge Pinto,Jorge Pinto
Linguarum Arena : Revista do Programa Doutoral em Didáctica de Línguas da Universidade do Porto , 2011,
Abstract: The main purpose of this article is to present a reflection on the Task Based Language Teaching (TBLT) and the focus on form approach due to the importance they currently have in the learning of an L2 and because they are thought to allow a more efficient and effective learning of Portuguese in Cape Verde. The TBLT aims to develop the students’ interlanguage through the execution of a task in which the students need the language to solve it. By resorting to the focus on form approach we want to call students’ attention to the linguistic forms that come up spontaneously in activities whose main focus is the meaning, which is the case of TBLT. Based on these theoretical principles, we present the partial results of a study that counted with the participation of three Cape Verdean teachers who have adopted the TBLT for a month and have included a focus on form approach for the teaching of grammar as an alternative to the more traditional teaching that predominates in the language less the main purpose of this article is to present a reflection on the Task Based Language Teaching (TBLT) and the focus on form approach due to the importance they currently have in the learning of an L2 and because they are thought to allow a more efficient and effective learning of Portuguese in Cape Verde. The TBLT aims to develop the students’ interlanguage through the execution of a task in which the students need the language to solve it. By resorting to the focus on form approach we want to call students’ attention to the linguistic forms that come up spontaneously in activities whose main focus is the meaning, which is the case of TBLT. Based on these theoretical principles, we present the partial results of a study that counted with the participation of three Cape Verdean teachers who have adopted the TBLT for a month and have included a focus on form approach for the teaching of grammar as an alternative to the more traditional teaching that predominates in the language lessons.
Quantitative promoter methylation analysis of multiple cancer-related genes in renal cell tumors
Vera L Costa, Rui Henrique, Franclim R Ribeiro, Mafalda Pinto, Jorge Oliveira, Francisco Lobo, Manuel R Teixeira, Carmen Jerónimo
BMC Cancer , 2007, DOI: 10.1186/1471-2407-7-133
Abstract: A panel of 18 gene promoters was assessed by quantitative methylation-specific PCR (QMSP) in 85 primarily resected renal tumors representing the four major histologic subtypes (52 clear cell (ccRCC), 13 papillary (pRCC), 10 chromophobe (chRCC), and 10 oncocytomas) and 62 paired normal tissue samples. After genomic DNA isolation and sodium bisulfite modification, methylation levels were determined and correlated with standard clinicopathological parameters.Significant differences in methylation levels among the four subtypes of renal tumors were found for CDH1 (p = 0.0007), PTGS2 (p = 0.002), and RASSF1A (p = 0.0001). CDH1 hypermethylation levels were significantly higher in ccRCC compared to chRCC and oncocytoma (p = 0.00016 and p = 0.0034, respectively), whereas PTGS2 methylation levels were significantly higher in ccRCC compared to pRCC (p = 0.004). RASSF1A methylation levels were significantly higher in pRCC than in normal tissue (p = 0.035). In pRCC, CDH1 and RASSF1A methylation levels were inversely correlated with tumor stage (p = 0.031) and nuclear grade (p = 0.022), respectively.The major subtypes of renal epithelial neoplasms display differential aberrant CDH1, PTGS2, and RASSF1A promoter methylation levels. This gene panel might contribute to a more accurate discrimination among common renal tumors, improving preoperative assessment and therapeutic decision-making in patients harboring suspicious renal masses.Cancer is currently a major cause of morbidity and mortality in Western World, only surpassed by cardiovascular diseases [1]. Kidney cancer accounts for approximately three percent of all solid neoplasms and its incidence appears to be rising [2]. Overall, it is estimated that 208 480 new cases of kidney cancer were diagnosed worldwide in 2002, with a total of 101 895 deaths [1]. In the USA, the adjusted incidence and mortality of this disease were approximately two times higher in males than in females, standing as the 7th leading malignant condition
Produ??o de biomassa e teor de óleo essencial em fun??o da idade e época de colheita em plantas de hortel?-japonesa
Chagas, Jorge Henrique;Pinto, Jose Eduardo Brasil Pereira;Bertolucci, Suzan Kelly Vilela;Santos, Fúlvia Maria do;
Acta Scientiarum. Agronomy , 2011, DOI: 10.4025/actasciagron.v33i2.5698
Abstract: this present work aimed to evaluate the effect of period and age the plants on harvest, dry biomass production, essential oil percentage content and storage period of mint (mentha arvensis l.) plants. two experiments were set: the first evaluated the effect of three harvest ages (80, 100 and 120 days) after transplanting, and three ages at the second harvest (60, 75 and 90 days). a randomized blocks design was used, with four replications in a 3 x 3 factorial scheme. in the second experiment, three harvest periods were evaluated - early january, late march and early june. the harvested material was dried at 37°c, the dry biomass of the aerial parts was calculated, the essential oil was extracted in a modified clevenger instrument and its percentage contents were calculated. plant age at first harvest did influence essential oil content and dry biomass of the aerial parts in response to plant age at the second harvest. the harvest period in which the essential oil content was higher was in early june.
Efeitos da luz e temperatura na germina??o de sementes de quatro espécies de plantas daninhas do gênero Digitaria
Mondo, Vitor Henrique Vaz;Carvalho, Saul Jorge Pinto de;Dias, Ana Carolina Ribeiro;Marcos Filho, Júlio;
Revista Brasileira de Sementes , 2010, DOI: 10.1590/S0101-31222010000100015
Abstract: brazil has the largest number of native species of digitaria in the americas. this genus is considered as a weed causing problems in various economic crops and can be found throughout brazil in many different ecological situations. the object of this research was to evaluate the effects of light and temperature on the seed germination of four digitaria weed species. the experiment was conducted in germination chambers using a factorial 4 x 2 scheme of treatments under four temperatures (25 oc constant, alternating 20-30 oc, 20-35 oc and 15-35 oc) with alternate light cycles (8h light/16h darkness) or absolute darkness. the weed species studied were digitaria horizontalis willd., d. bicornis (lam.) roem. & schult., d.ciliaris (retz.) koel. and d. insularis (l.) fedde. the percentage and speed of germination were evaluated up to 21 days after the start of the germination test. the four species showed differing seed physiological responses. d. bicornis and d. horizontalis required light for germination while d. ciliaris and d. insularis did not. the best temperatures for germination are 20-35 oc for d. bicornis and d. ciliaris, and 20-35 oc or 15-35 oc for d. horizontalis and d. insularis.
Um Modelo para Gera o de Perfis de Usuários Baseado em Técnicas de Psicometria
Marcelo Henrique Euzebio Batista,Jo?o Ricardo Bittencourt,Jorge Luis Victória Barbosa,Sergio Crespo C S Pinto
Revista Brasileira de Informática na Educa??o , 2010,
Abstract: Este trabalho prop e um modelo para gera o de perfis de usuários baseado em Psicometria, propiciando a jun o entre áreas distintas, como Psicologia e Computa o. O PPG (Psychometric Profile Generator), assim denominado, consiste em um modelo computacional para gera o de perfis de usuários, prospectando o nível de habilidade ou comportamento dos avaliados através do modelo matemático TRI – Teoria de Resposta ao Item. O PPG fornece aos sistemas externos de educa o, entretenimento e recrutamento e sele o o nível de habilidade ou comportamento n o tendo nenhum contato com os usuários desses sistemas. Sendo assim o principal foco do PPG é fornecer um perfil na forma de um nível de habilidade e ou comportamento, valendo-se de conhecimentos de áreas distintas, como os modelos matemáticos da Psicometria e técnicas de Inferências Estatísticas.
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