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Search Results: 1 - 10 of 179394 matches for " John H. Teeter "
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Phylogenetic Analysis and Taste Cell Expression of Calpain 9 in Catfish (Ictalurus punctatus)  [PDF]
Tetsuya Ookura, Eiki Koyama, Anne Hansen, John H. Teeter, Yukio Kawamura, Joseph G. Brand
Natural Science (NS) , 2015, DOI: 10.4236/ns.2015.73016
Abstract: The calpains, calcium-activated neutral proteases, play important roles in calcium-regulated intra-cellular signal transduction cascades. Here we report the isolation and initial characterization of a cDNA encoding a calpain 9, digestive tract specific calpain, from catfish taste epithelium. This calpain 9 (Ip-CAPN9a) shares 61% identity with human calpain 9. Phylogenetic analysis provides evidence that catfish calpain 9 and the related enzymes from Oncorhynchus mykiss, Danio rerio, Xenopus laevis, Mus musculus, Rattus norvegicus and Homo sapiens make up a distinct clade within the tissue-specific calpain family. Northern blot analysis reveals that Ip-CAPN9a is predominantly expressed in barbell and digestive tract, but not expressed in brain. An antibody against the N-terminal segment of Ip-CAPN9a recognizes cells within the taste buds in catfish barbells.
Biochemical enrichment and biophysical characterization of a taste receptor for L-arginine from the catfish, Ictalurus puntatus
William Grosvenor, Yuri Kaulin, Andrew I Spielman, Douglas L Bayley, D Lynn Kalinoski, John H Teeter, Joseph G Brand
BMC Neuroscience , 2004, DOI: 10.1186/1471-2202-5-25
Abstract: Both PHA-E and RCA-I almost exclusively labeled an 82–84 kDa protein band of an SDS-PAGE of solubilized barbel taste epithelial membranes. Further, both rhodamine-conjugated RCA-I and polyclonal antibodies raised to the 82–84 kDa electroeluted peptides labeled the apical region of catfish taste buds. Because of the specificity shown by RCA-I, lectin affinity was chosen as the first of a three-step procedure designed to enrich the presumed LGICR for L-Arg. Purified and CHAPS-solubilized taste epithelial membrane proteins were subjected successively to (1), lectin (RCA-I) affinity; (2), gel filtration (Sephacryl S-300HR); and (3), ion exchange chromatography. All fractions from each chromatography step were evaluated for L-Arg-induced ion channel activity by reconstituting each fraction into a lipid bilayer. Active fractions demonstrated L-Arg-induced channel activity that was inhibited by D-arginine (D-Arg) with kinetics nearly identical to those reported earlier for L-Arg-stimulated ion channels of native barbel membranes reconstituted into lipid bilayers. After the final enrichment step, SDS-PAGE of the active ion channel protein fraction revealed a single band at 82–84 kDa which may be interpreted as a component of a multimeric receptor/channel complex.The data are consistent with the supposition that the L-Arg receptor is a LGICR. This taste receptor remains active during biochemical enrichment procedures. This is the first report of enrichment of an active LGICR from the taste system of vertebrata.The initial event in taste transduction involves recognition of taste stimuli by plasma membrane-associated receptor proteins. These proteins are concentrated at the apical end of specialized neuro-epithelial cells (taste cells) found within multicellular end-organs known as taste buds [1,2]. The recognition binding sites for most taste stimuli face the exterior environment. The interaction of a taste stimulus with this recognition site triggers a chain of metabolic an
The sea lamprey Petromyzon marinus genome reveals the early origin of several chemosensory receptor families in the vertebrate lineage
Scot Libants, Kevin Carr, Hong Wu, John H Teeter, Yu-Wen Chung-Davidson, Ziping Zhang, Curt Wilkerson, Weiming Li
BMC Evolutionary Biology , 2009, DOI: 10.1186/1471-2148-9-180
Abstract: Petromyzon is the only extant jawless vertebrate whose genome has been sequenced. Known to be exquisitely sensitive to several classes of odorants, lampreys detect fewer amino acids and steroids than teleosts. This reduced number of detectable odorants is indicative of reduced numbers of CR gene families or a reduced number of genes within CR families, or both, in the sea lamprey. In the lamprey genome we identified a repertoire of 59 intact single-exon CR genes, including 27 OR, 28 TAAR, and four V1R-like genes. These three CR families were expressed in the olfactory organ of both parasitic and adult life stages.An extensive search in the lamprey genome failed to identify potential orthologs or pseudogenes of the multi-exon V2R family that is greatly expanded in teleost genomes, but did find intact calcium-sensing receptors (CASR) and intact metabotropic glutamate receptors (MGR). We conclude that OR and V1R arose in chordates after the cephalochordate-urochordate split, but before the diversification of jawed and jawless vertebrates. The advent and diversification of V2R genes from glutamate receptor-family G protein-coupled receptors, most likely the CASR, occurred after the agnathan-gnathostome divergence.While the general features of the olfactory system are remarkably conserved among vertebrates, the chemosensory receptor gene families expressed there have experienced dramatic diversification [1]. Odor detection and discrimination is accomplished by the interaction of odorous ligands with receptors located on the cilia or microvilli found on the dendritic ends of olfactory receptor neurons (ORNs), and will necessarily depend upon the number of individual receptors and their specificities [2]. In this paper we use the term chemosensory receptor (CR) to refer to all known G protein-coupled receptors that interact with odorant molecules in olfactory epithelia. CR genes have been a subject of much interest since their discovery, because of their persistence in div
Avalia??o das Propriedades do ácido Nicotínico no Desempenho e no Balan?o Térmico de Frangos de Corte Durante Estresse por Calor
Ribeiro, AML;Mahmoud, H;Teeter, RG;Penz Jr, AM;
Revista Brasileira de Ciência Avícola , 2001, DOI: 10.1590/S1516-635X2001000100004
Abstract: four doses of nicotinic acid, added in the drinking water of broilers, were offered in order to study its effect on performance and heat production in heat stress conditions (6 h of 35° c). doses were 15, 100, 330 and 1000 mg/l. there was no effect of dose on weight gain, feed intake, feed efficiency, water intake and rectal temperature. slight changes due to the doses of nicotinic acid and heat stress in the blood parameters of the birds were noted. heat production (kcal/h/metabolic weight) decreased in 100 and 1000 mg/l dose as compared to 15 mg/l dose. there was no benefit using nicotinic acid in those doses in heat stress alleviation.
Avalia o das Propriedades do ácido Nicotínico no Desempenho e no Balan o Térmico de Frangos de Corte Durante Estresse por Calor
Ribeiro AML,Mahmoud H,Teeter RG,Penz Jr AM
Revista Brasileira de Ciência Avícola , 2001,
Abstract: Foram oferecidas 4 doses de ácido nicotínico (AN) na água de beber de frangos de corte, a fim de estudar seu efeito no desempenho e na produ o de calor em condi es de estresse por calor (6 h de 35o C). As doses foram 15, 100, 330 e 1000 mg/L. N o houve efeito do AN nas doses usadas para ganho de peso, consumo de alimento, eficiência alimentar, consumo de água e temperatura retal. Foram observadas algumas altera es devido ao AN e estresse por calor nos parametros sangüíneos das aves. A produ o de calor (kcal/h/peso metabólico) diminuiu com o uso de 100 e 1000 mg/L comparados com 15 mg/L. Nas dosagens usadas n o foi observado efeito benéfico do AN no controle do estresse por calor.
Simulation of 5-Fluorouracil Intercalated into Montmorillonite Using Spartan ’14: Molecular Mechanics, PM3, and Hartree-Fock  [PDF]
John H. Summerfield
Open Journal of Physical Chemistry (OJPC) , 2015, DOI: 10.4236/ojpc.2015.53006

Molecular mechanics calculations, based on equations such as the one below, are used to investigate a colorectal cancer drug, 5-fluorouracil, intercalated into a clay, montmorillonite. This combination is currently being considered as a drug delivery system. The swelling of clays has been studied since the 1930s and is still not fully understood. Spartan ’14 is used for the calculations. Semi-empirical and ab initio basis set scaling is also examined since there are roughly 300 atoms involved in the full model.


The Circle, from Descartes’ Point of View  [PDF]
John H. Dreher
Open Journal of Philosophy (OJPP) , 2017, DOI: 10.4236/ojpp.2017.72012
Abstract: My project is to show that despite relentless criticism from the beginning, Descartes cannot be reasonably faulted in his attempt to relieve systematic doubt or as it is sometimes called, metaphysical doubt. By “cannot be reasonably faulted” I mean that Descartes is not open criticism either for having made assumptions that were unwarranted by standards of his own time or for having made errors in reasoning. The paper seeks to analyze Descartes’ struggle with metaphysical doubt and radical skepticism in relation to issues concerning provability and truth in arithmetic that are addressed by G?del-L?b provability logic.
Hume, Newton and Malebranche on Causation  [PDF]
John H. Dreher
Open Journal of Philosophy (OJPP) , 2017, DOI: 10.4236/ojpp.2017.73018
Abstract: Hume, Newton and Malebranche have been made out to be an odd couple, united in the belief that prior Aristotelian and Cartesian analyses of causation are inadequate; yet differing in the correctives they offer to relieve those earlier misconceptions. Malebranche famously appeals to God to validate his occasionalist account of causation. Newton appeals to God to explain forces like gravity which is revealed by necessary laws like the Law of Universal Gravitation. Hume, on the other hand, seeks to explain causation as a natural phenomenon that can be explained by his theory of belief formation. This paper argues that the difference between Malebranche and Hume runs much deeper than their substantive analyses of causation; they also differ about experimental reasoning and natural philosophy in which Newton makes room for God as an integral part of natural philosophy but not experimental science whereas Malebranche thinks of God as an essential part of experimental science. More specifically, this paper hopes to make original contributions by arguing 1) that although Hume does not think of the grand laws of nature (from Galileo and Newton) as necessities but rather as mere universal regularities, like “the sun will rise tomorrow” and “all men must dye”, he carefully makes room for a unique place for the grand laws of physics by distinguishing quotidian regularities from those grand laws by virtue of their specificity and comprehensiveness. This paper also argues 2) that Hume conceived of the grand laws of nature as operating in ideal circumstances, which explains why it is that apparent exceptions to those laws do not undermine them. The paper argues in conclusion that 3) Hume is not “Malebranche minus God”, that 4) Newton is not in a better position than Hume to contemplate nature’s twists and turns and finally that 5) to imagine a time at which the sun does not rise or a man who simply does not ever die is not to imagine a weird and inexplicable anomaly but rather to imagine that the entire course of nature has changed.
Vesikovaginale Fistel: Rekonstruktive Techniken
John H
Journal für Urologie und Urogyn?kologie , 2005,
Abstract: Die vesikovaginale Fistel ist in den Entwicklungsl ndern leider noch immer h ufig und hat in Westafrika eine geburtshilfliche Inzidenz von ca. 3/1.000 Geburten. Die fehlende M glichkeit einer transurethalen Katheterisierung und Einleitung eines Kaiserschnittes sind die Gründe von Gewebsisch mie in einer verl ngerten Austreibungsphase. In Europa entsteht die vesikovaginale Fistel am h ufigsten iatrogen nach Kleinbeckenchirurgie, in 75 % nach gyn kologischen Eingriffen. Jede vesikovaginale Fistel mit Leitsymptom der absoluten Harininkontinenz bedeutet für die betroffene Frau eine massive Einschr nkung der Lebensqualit t und geht hin bis zur sozialen Isolation. Ein Spontanverschlu ist nur selten. Ein operativer Fistelverschlu mit vaginalem oder transabdominalen Zugang führt bei Respektierung von einigen Grundprinzipien der vesikovaginalen Fistelchirurgie in hohem Prozentsatz zum definitiven Verschlu .
Mikroskopische Vasovasostomie
John H
Journal für Urologie und Urogyn?kologie , 2013,
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