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Search Results: 1 - 10 of 219386 matches for " John D. McNeish "
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Derivation of High Purity Neuronal Progenitors from Human Embryonic Stem Cells
Gabriel Nistor, Monica M. Siegenthaler, Stephane N. Poirier, Sharyn Rossi, Aleksandra J. Poole, Maura E. Charlton, John D. McNeish, Chris N. Airriess, Hans S. Keirstead
PLOS ONE , 2011, DOI: 10.1371/journal.pone.0020692
Abstract: The availability of human neuronal progenitors (hNPs) in high purity would greatly facilitate neuronal drug discovery and developmental studies, as well as cell replacement strategies for neurodegenerative diseases and conditions, such as spinal cord injury, stroke, Parkinson's disease, Alzheimer's disease, and Huntington's disease. Here we describe for the first time a method for producing hNPs in large quantity and high purity from human embryonic stem cells (hESCs) in feeder-free conditions, without the use of exogenous noggin, sonic hedgehog or analogs, rendering the process clinically compliant. The resulting population displays characteristic neuronal-specific markers. When allowed to spontaneously differentiate into neuronal subtypes in vitro, cholinergic, serotonergic, dopaminergic and/or noradrenergic, and medium spiny striatal neurons were observed. When transplanted into the injured spinal cord the hNPs survived, integrated into host tissue, and matured into a variety of neuronal subtypes. Our method of deriving neuronal progenitors from hESCs renders the process amenable to therapeutic and commercial use.
Novel Pancreatic Endocrine Maturation Pathways Identified by Genomic Profiling and Causal Reasoning
Alex Gutteridge, J. Michael Rukstalis, Daniel Ziemek, Mark Tié, Lin Ji, Rebeca Ramos-Zayas, Nancy A. Nardone, Lisa D. Norquay, Martin B. Brenner, Kim Tang, John D. McNeish, Rebecca K. Rowntree
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0056024
Abstract: We have used a previously unavailable model of pancreatic development, derived in vitro from human embryonic stem cells, to capture a time-course of gene, miRNA and histone modification levels in pancreatic endocrine cells. We investigated whether it is possible to better understand, and hence control, the biological pathways leading to pancreatic endocrine formation by analysing this information and combining it with the available scientific literature to generate models using a casual reasoning approach. We show that the embryonic stem cell differentiation protocol is highly reproducible in producing endocrine precursor cells and generates cells that recapitulate many aspects of human embryonic pancreas development, including maturation into functional endocrine cells when transplanted into recipient animals. The availability of whole genome gene and miRNA expression data from the early stages of human pancreatic development will be of great benefit to those in the fields of developmental biology and diabetes research. Our causal reasoning algorithm suggested the involvement of novel gene networks, such as NEUROG3/E2F1/KDM5B and SOCS3/STAT3/IL-6, in endocrine cell development We experimentally investigated the role of the top-ranked prediction by showing that addition of exogenous IL-6 could affect the expression of the endocrine progenitor genes NEUROG3 and NKX2.2.
End Stage Renal Disease Economics and the Balance of Treatment Modalities  [PDF]
John D. Sullivan
Journal of Service Science and Management (JSSM) , 2010, DOI: 10.4236/jssm.2010.31005
Abstract: Periodically, research articles emerge arguing the economic benefits of peritoneal dialysis, or PD, over the traditional in-center hemo dialysis for patients suffering from End Stage Renal Disease. Resulting conclusions indentify PD as the ideal therapy to reduce Medicare expenditures for this expensive treatment. However, despite this possible economic benefit to the United States taxpayer, the number of PD patients remains relatively flat with an increasing amount of patients being prescribed in-center hemo dialysis. A simplistic view of controlling the rising costs, on a per treatment basis, associated with the treatment of this disease would be to increase the number of patients from in-center hemo dialysis to peritoneal dialysis. This paper will argue why this shift is both unlikely and unrealistic, and why the resulting potential cost savings to this segment of the Medicare program is a myth.
Model of plasmon excitations in a bundle and two-dimensional array of nanotubes
Tibab McNeish,Godfrey Gumbs,Antonios Balassis
Physics , 2010, DOI: 10.1103/PhysRevB.77.235440
Abstract: We calculate the plasma excitations in a bundle as well as a two-dimensional (2D) periodic array of aligned parallel multishell nanotubes on a substrate. The carbon nanotubes are oriented perpendicular to the substrate. The model we use for the system is an electron gas confined to the surface of an infinitely long cylinder embedded in a background dielectric medium. Electron tunneling between individual tubules is neglected. We include the Coulomb interaction between electrons on the same tubule and on different tubules for the same nanotube and neighboring nanotubes. We present a self-consistent field theory for the dispersion equation for intrasubband and intersubband plasmon excitations. For both the bundle and 2D array of aligned parallel nanotubes, the dispersion relation of the collective modes is determined by a three-dimensional wave vector with components in the direction of the nanotube axes and in the transverse directions. The dispersion equation is solved numerically for a singlewall nanotube 2D array as well as a bundle, and the plasmon excitation energies are obtained as a function of wave vector. The intertube Coulomb interaction couples plasmons with different angular momenta in individual nanotubes, lifting the degeneracy of the single-nanotube modes. This effect is analyzed numerically as a function of the separation between the tubules. We show that the translational symmetry of the lattice is maintained in the plasmon spectrum for the periodic array, and the plasmon energies have a periodic dependence on the transverse wave vector. For the bundle, the Coulomb interaction between nanotubes gives rise to optical plasmon excitations.
Valuing European Put Options under Skewness and Increasing [Excess] Kurtosis  [PDF]
John-Peter D. Chateau
Journal of Mathematical Finance (JMF) , 2014, DOI: 10.4236/jmf.2014.43015

To capture the impact of skewness and increase kurtosis on Black’s [1] European put values, we first substitute a Gram-Charlier (GC) distribution and next a Johnson distribution for Black’s Gaussian one. We introduce next each distribution in the option payoff and develop until the closedform expression of each put is arrived at. Finally, we estimate by simulations GC, Johnson and Black put options, choosing the latter one as benchmark. Simulation estimates encompassing both skewness and kurtosis show that, for at-the-money (ATM) or slightly in-the-money put values, 1) Black’s overvaluation with respect to Johnson puts is very significant and 2) its undervaluation with respect to GC ones remains moderate. Yet, by using the same skewness values for both GC and Johnson puts, we highlight the differences induced by increasing kurtosis between the two models. In this case, the GC overvaluation for ATM values is explained by value differences in the put time component. Yet, while both Black and GC values exhibit significant time decay close to expiry, Johnson’s ones remain stable up to maturity.

Pricing the Credit-Risk Put Embedded in Borrowers’ Extendible Credit Commitments, with Its Application to Basel-3 Micro-Prudential Regulation  [PDF]
John-Peter D. Chateau
Journal of Mathematical Finance (JMF) , 2016, DOI: 10.4236/jmf.2016.65052
Abstract: This research makes two contributions: 1) use a term structure framework to price analytically the put option implicit in borrowers’ extendible credit commitments and 2) use the latter to compute in a ratings-based model the capital charge corresponding to the credit-risk exposure of such commitments. Since the term structure of interest rates is stochastic, the zero-coupon bonds in the put closed-form solution delink discounting factor from the credit and funding rates that define the credit spread appearing in the put payoff. By essence, extendible commitments straddle the term-based commitment classification of Basel-3 simplified approach. To improve this, we formulate a ratings-based model that combines extendible put values with new coefficients (forward funding proportion and exposure at funding) as well as a matrix that captures credit-ratings migration over time. Moreover, the combination is versatile enough to deal with a borrower’s credit downgrade and its attendant incremental Basel-3 capital charge.
Measurement of Arsenic Species in Infant Rice Cereals by Liquid Chromatography Inductively Coupled Plasma Mass Spectrometry  [PDF]
John D. Brockman, John W. N. Brown IV
American Journal of Analytical Chemistry (AJAC) , 2012, DOI: 10.4236/ajac.2012.310091
Abstract: Infant rice cereals were analyzed for total arsenic, inorganic arsenic (i-As) and the organic arsenic species monomethylarsonoic acid (MMA) and dimethylarsinic acid (DMA) using liquid chromatography inductively coupled plasma mass spectrometry (LC-ICP-MS). Total arsenic concentrations in the samples ranged from 110 ng/gup to 420 ng/g. The i-As in the rice cereals accounted for 33% to 77% of the total arsenic. The observed variability between infant rice cereals makes a dietary survey approach to accessing arsenic exposures difficult.
Infection Prevalence of Borrelia burgdorferi in Ticks Collected from Songbirds in Far-Western Canada  [PDF]
John D. Scott, Lance A. Durden, John F. Anderson
Open Journal of Animal Sciences (OJAS) , 2015, DOI: 10.4236/ojas.2015.53027
Abstract: Worldwide, wild birds play a vital role in the dispersal of ticks that harbour tick-borne pathogens, including Borrelia burgdorferi, the Lyme disease bacterium. Using PCR testing, we found 124 (31%) of 405 ticks (4 species), which were collected from 21 species of birds in far-western Canada, to be infected with B. burgdorferi. Transstadial transmission of B. burgdorferi occurred from larva to nymph, plus nymph to adult, in the avian coastal tick, Ixodes auritulus, collected from songbirds in British Columbia (B.C). Collectively, all 3 motile life stages (larva, nymph, adult) of this tick had an infection prevalence of 31% for B. burgdorferi, which suggests vector competency. A Pacific Wren was highly infested with I. auritulus immatures, and 20 (44%) of 45 ticks (2 nymphs, 43 larvae) were infected with B. burgdorferi. This heavy infestation shows the high potential to initiate a new population of ticks and to disseminate Lyme spirochetes. Epidemiologically, B. burgdorferi-infected I. auritulus larvae collected from the Spotted Towhee, Swainson’s Thrush, Pacific Wren, and Fox Sparrow suggest that these avian hosts act as reservoirs for B. burgdorferi. In this study, the western blacklegged tick, Ixodes pacificus, and Ixodes spinipalpis played a limited role in the enzootic transmission cycle of B. burgdorferi along coastal B.C. We document the first record of I. spinipalpis on a bird in Alberta. Because songbirds widely disperse Lyme disease vector ticks, primary health providers and the general public must be vigilant that outdoors people may be bitten by B. burgdorferi-infected ticks throughout far-western Canada.
Regulation of KCa2.3 and endothelium-dependent hyperpolarization (EDH) in the rat middle cerebral artery: the role of lipoxygenase metabolites and isoprostanes
Kathryn M. Gauthier,William B. Campbell,Alister J. McNeish
PeerJ , 2015, DOI: 10.7717/peerj.414
Abstract: Background and Purpose. In rat middle cerebral arteries, endothelium-dependent hyperpolarization (EDH) is mediated by activation of calcium-activated potassium (KCa) channels specifically KCa2.3 and KCa3.1. Lipoxygenase (LOX) products function as endothelium-derived hyperpolarizing factors (EDHFs) in rabbit arteries by stimulating KCa2.3. We investigated if LOX products contribute to EDH in rat cerebral arteries.
Update of the Chronology of Natural Signals in the Near-Surface Mean Global Temperature Record and the Southern Oscillation Index  [PDF]
Chris R. de Freitas, John D. McLean
International Journal of Geosciences (IJG) , 2013, DOI: 10.4236/ijg.2013.41A020

Time series for the Southern Oscillation Index and mean global near surface temperature anomalies are compared for the 1950 to 2012 period using recently released HadCRU4 data. The method avoids a focused statistical analysis of the data, in part because the study deals with smoothed data, which means there is the danger of spurious correlations, and in part because the El Ni?o Southern Oscillation is a cyclical phenomenon of irregular period. In these situations the results of regression analysis or similar statistical evaluation can be misleading. With the potential controversy arising over a particular statistical analysis removed, the findings indicate that El Nino-Southern Oscillation exercises a major influence on mean global temperature. The results show the potential of natural forcing mechanisms to account for mean global temperature variation, although the extent of the influence is difficult to quantify from among the variability of short-term influences.

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