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Search Results: 1 - 10 of 135820 matches for " Jo?o Bosco Lima;Bastos "
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Simultaneous circulation of all four dengue serotypes in Manaus, State of Amazonas, Brazil in 2011
Bastos, Michele de Souza;Figueiredo, Regina Maria Pinto de;Ramasawmy, Rajendranath;Itapirema, Evaulino;Gimaque, Joo Bosco Lima;Santos, Lucilaide Oliveira;Figueiredo, Luiz Tadeu Moraes;Mour?o, Maria Paula Gomes;
Revista da Sociedade Brasileira de Medicina Tropical , 2012, DOI: 10.1590/S0037-86822012000300022
Abstract: introduction: manaus, the capital city of the state of amazon with nearly 2 million inhabitants, is located in the middle of the amazon rain forest and has suffered dengue outbreaks since 1998. methods: in this study, blood samples were investigated using reverse transcriptase-polymerase chain reaction (rt-pcr), aimed at identifying dengue virus serotypes. results: acute phase sera from 432 patients were tested for the presence of dengue virus. out of the 432 patients, 137 (31.3%) were found to be positive. all the four dengue virus serotypes were observed. conclusions: the simultaneous circulation of the four dengue serotypes is described for the first time in manaus and in brazil.
Serological evidence of hantavirus infection in rural and urban regions in the state of Amazonas, Brazil
Gimaque, Joo Bosco Lima;Bastos, Michele de Souza;Braga, Wornei Silva Miranda;Oliveira, Cintia Mara Costa de;Castilho, Márcia da Costa;Figueiredo, Regina Maria Pinto de;Galusso, Elizabeth dos Santos;Itapirema, Evaulino Ferreira;Figueiredo, Luiz Tadeu Moraes de;Mour?o, Maria Paula Gomes;
Memórias do Instituto Oswaldo Cruz , 2012, DOI: 10.1590/S0074-02762012000100019
Abstract: hantavirus disease is caused by the hantavirus, which is an rna virus belonging to the family bunyaviridae. hantavirus disease is an anthropozoonotic infection transmitted through the inhalation of aerosols from the excreta of hantavirus-infected rodents. in the county of itacoatiara in the state of amazonas (am), brazil, the first human cases of hantavirus pulmonary and cardiovascular syndrome were described in july 2004. these first cases were followed by two fatal cases, one in the municipality of maués in 2005 and another in itacoatiara in 2007. in this study, we investigated the antibody levels to hantavirus in a population of 1,731 individuals from four different counties of am. sera were tested by igg/igm- enzyme-linked immune-sorbent assay using a recombinant nucleocapsid protein of the araraquara hantavirus as an antigen. ten sera were igg positive to hantavirus (0.6%). among the positive sera, 0.8% (1/122), 0.4% (1/256), 0.2% (1/556) and 0.9% (7/797) were from atalaia do norte, careiro castanho, itacoatiara and lábrea, respectively. none of the sera in this survey were igm-positive. because these counties are distributed in different areas of am, we can assume that infected individuals are found throughout the entire state, which suggests that hantavirus disease could be a local emerging health problem.
Clinical and Virological Descriptive Study in the 2011 Outbreak of Dengue in the Amazonas, Brazil
Valquiria do Carmo Alves Martins, Michele de Souza Bastos, Rajendranath Ramasawmy, Regina Pinto de Figueiredo, Joo Bosco Lima Gimaque, Wornei Silva Miranda Braga, Mauricio Lacerda Nogueira, Sergio Nozawa, Felipe Gomes Naveca, Luiz Tadeu Moraes Figueiredo, Maria Paula Gomes Mour?o
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0100535
Abstract: Background Dengue is a vector-borne disease in the tropical and subtropical region of the world and is transmitted by the mosquito Aedes aegypti. In the state of Amazonas, Brazil during the 2011 outbreak of dengue all the four Dengue virus (DENV) serotypes circulating simultaneously were observed. The aim of the study was to describe the clinical epidemiology of dengue in Manaus, the capital city of the state of the Amazonas, where all the four DENV serotypes were co-circulating simultaneously. Methodology Patients with acute febrile illness during the 2011 outbreak of dengue, enrolled at the Funda??o de Medicina Tropical Dr. Heitor Viera Dourado (FMT-HVD), a referral centre for tropical and infectious diseases in Manaus, were invited to participate in a clinical and virological descriptive study. Sera from 677 patients were analyzed by RT-nested-PCRs for flaviviruses (DENV 1–4, Saint Louis encephalitis virus-SLEV, Bussuquara virus-BSQV and Ilheus virus-ILHV), alphavirus (Mayaro virus-MAYV) and orthobunyavirus (Oropouche virus-OROV). Principal Findings Only dengue viruses were detected in 260 patients (38.4%). Thirteen patients were co-infected with more than one DENV serotype and six (46.1%) of them had a more severe clinical presentation of the disease. Nucleotide sequencing showed that DENV-1 belonged to genotype V, DENV-2 to the Asian/American genotype, DENV-3 to genotype III and DENV-4 to genotype II. Conclusions Co-infection with more than one DENV serotype was observed. This finding should be warning signs to health authorities in situations of the large dispersal of serotypes that are occurring in the world.
Pajé: reconstru??o e sobrevivência
Botelho, Joo Bosco;Costa, Hideraldo Lima da;
História, Ciências, Saúde-Manguinhos , 2006, DOI: 10.1590/S0104-59702006000400009
Abstract: on the brazilian coast and in the amazon, pajés played a role in the resolution of problems from the sixteenth through eighteenth centuries, although curing diseases was only one of their functions. colonial laws were designed to ensure the legitimacy of the conquest, and right from their earliest long-term contacts with the natives, missionaries and settlers saw the pajés as major roadblocks to proposed changes. therefore, from the times of the colony through the empire and the republic, ecclesiastic and lay leaders worked together to bring about the physical and moral downfall of the pajés. the ethnic and linguistic changes that wiped out hundreds of languages have always hampered efforts to characterize the figure of the pajé in clear terms. with european specialists making their influence felt, pajés have been confused with asian shamans. but the pajé has reconstructed himself and survived. despite the violent transformations caused by salesian missionaries in the upper negro river area, pajés there continue exercising their powers even today.
Tuberculosis in hospital environment: clinical profile in a tertiary hospital from Ceará and level of knowledge of health personnel about control measures
Roberto da Justa Pires Neto,Carolina Oliveira Costa,Joo Bosco Breckenfeld Bastos Filho,Juliana de Melo Matos Lins
Revista Brasileira em Promo??o da Saúde , 2010,
Abstract: Objectives: To describe clinical and epidemiological characteristics of inpatients with tuberculosis (TB) and to assess the knowledge of health personnel on fundamental concepts about TB and control measures for pulmonary tuberculosis in a hospital environment. Methods: The study was conducted in a tertiary hospital in Fortaleza-CE and involved patients admitted with TB and health professionals responsible for assistance. A first phase was characterized by a retrospective study of medical records of patients admitted with suspected TB. In a second stage, a cross-sectional study with application of a structured questionnaire assessed the knowledge of health personnel on TB control measures in a hospital environment. Results: Sixty-seven patients admitted with suspected TB had their medical records assessed. Among the confirmed cases, the most frequent clinical form was pulmonary (81.3%). Out of 55 patients admitted with suspected pulmonary tuberculosis, only 29 (52.7%) were admitted in a respiratory isolation bed. Twenty-six patients with suspected pulmonary tuberculosis on admission stayed a total of 148 days out of a respiratory isolation bed (average 4.1 days / patient). The knowledge of 159 health professionals about TB was assessed. Regarding the transmission of TB, 107 (67.2%) were unaware of airborne transmission and 109 (68.5%) ignored the clinical forms that require respiratory isolation. Conclusions: Pulmonary tuberculosis is the most frequent clinical form among inpatients in a tertiary hospital in Fortaleza-CE. Considerable fraction of health personnel doesn’t know key concepts related to tuberculosis and essential for the proper and safe care. Descriptors: Tuberculosis; Infectious Disease Transmission; Exposure to Biological Agents; Health personnel.
Natural selection for efficiency in the use of nitrogen in common bean identified by analysis of microsatellites and grain yield
Leite,Monik Evelin; Lima,Igor Almeida; Pereira,Dyanna Rangel; Santos,Joo Bosco dos;
Electronic Journal of Biotechnology , 2012, DOI: 10.2225/vol15-issue6-fulltext-6
Abstract: this study was carried out in order to verify whether natural selection acts on segregating common bean populations grown in the presence and absence of n, and to verify that natural selection affects the frequency of microsatellite alleles specifically, and in order to identify those that can assist in selection. four populations from the crosses ouro negro x ci-107 and vc-5 x iapar-81 were carried forward in bulk to f8 at both levels of n. ouro negro and vc-5 are considered stress tolerant to nitrogen and iapar-81 and ci-107 responsive. one hundred progenies were obtained from each cross and grown in the presence and absence of n. dna was extracted from 400 progenies, of which 194 were evaluated in the rainy season and 79 in the winter crop for grain yield. these progenies were evaluated in two separate experiments with and without n, in a 14 x 14 lattice design during rainy season and 9 x 9 in the winter season. we selected 35 pairs of polymorphic primers from the parents, with 20 being common from the two crosses. using dna of the 400 progenies, it was found that natural selection acted in 33 loci, in at least one environment and cross, and that alleles of the four parents were favoured by natural selection. qtls were identified for the response rate of n and for grain yield and the linked markers are potential for assisted selection, especially the two most stable; bmd-20, in response to the use of n and pvbr-93 for grain yield.
Cenourete e Catetinho: minicenouras brasileiras
Lana, Milza M.;Vieira, Jairo V.;Silva, Joo Bosco C.;Lima, Dejoel B.;
Horticultura Brasileira , 2001, DOI: 10.1590/S0102-05362001000300019
Abstract: brazilian carrot production is around 750,000 tons per year. about 10% of this total is composed of thin roots graded as 1a type. depending on the production system, planting date, and growing region, the percentage of 1a roots can reach about 20% of the total production. these 1a roots are cheaper than other grades, especially in the winter season, when the excess of production has been observed in certain growing areas of the country. aiming to add commercial value to these 1a roots, the embrapa hortali?as has developed equipment that is an adaptation of a potato peeler machine. this equipment allowed the development of a minimum processing technology for obtaining mini-carrots named as `cenourete' (a processing item similar to the american baby-carrot) and `catetinho' (a ball-shaped mini-carrot). both products are visually attractive to consumers, ready to eat either in natura (as a healthy snake) or cooked. the basic procedure to obtain these two products is to burnish cylindrical root segments by rubbing them against an abrasive surface until either an elliptical or spherical shape can be obtained. after this initial processing phase, a finishing procedure is carried out in order to polish the external surface of the root segment. finally the product is sanitized and packed preferentially under vacuum conditions. there is great expectation about increasing the per capita consumption of healthy processing items especially among children and workers who work outdoors. these two items are expected to supply part of this demand in brazil.
Sele??o fenotípica e assistida por marcadores moleculares de famílias de feijoeiro-comum com alta produtividade
Pereira, Helton Santos;Santos, Joo Bosco dos;Souza, Thaís Paula de;Lima, Igor Almeida;
Pesquisa Agropecuária Brasileira , 2008, DOI: 10.1590/S0100-204X2008001100014
Abstract: the objective of this work was to select common bean families with high grain yield, using phenotypic and marker-assisted selections (mas). evaluations were made for 394 families of four populations and their six parents, in lavras county, minas gerais, brazil. two field experiments were conducted: one in the f3:4 generation, in the rainy season of 2005/2006, using a 20x20 simple lattice design; and other in the f3:5 generation, in the dry season of 2006, using a 20x20 triple lattice design. genetic and phenotypic parameters were estimated, and the families were genotyped with microsatellite markers for grain yield quantitative trait loci (qtl), that had been identified previously. simple marker association analysis was performed between markers and grain yield, and an index was estimated for mas. the high variability between families and the heritability estimates made high genetic gains possible through phenotypic selection. the markers explained a small percentage of phenotypic variation, and presented high qtl x environment and qtl x population interactions. the mas achieved small gains, with low coincidence of families selected by both methodologies, showing, in this case, that the mas inefficiency was mainly due to the low number of available markers.
Efeitos da irriga??o e aduba??o nitrogenada sobre a massa de forragem de cultivares de Panicum maximum Jacq.
Souza, érica Matsumoto de;Isepon, Olair José;Alves, Joo Batista;Bastos, Joo Francisco P.;Lima, Ronaldo Cintra;
Revista Brasileira de Zootecnia , 2005, DOI: 10.1590/S1516-35982005000400008
Abstract: this trial was carried out from september 6th (2000) to september 18th (2001) to evaluate the effects of irrigation and nitrogen fertilization on drymatter yield of five different cultivars of panicum maximum. five cultivars (guiné, coloni?o, momba?a, tanzania and centauro) under three nitrogen rates (50, 75 and 100 kg/ha/cut of n), with and without irrigation were evaluated. the experimental treatments, the combination of a 5x3x2 factorial, was allocated in a completely randomized design with four replications. irrigation brought about significant drymatter (dm) yield of all cultivars. in the rainy season (october/00 to march/01), highest dm yield was observed on plot receiving 75 and 100 kg/ha/cut of n. under irrigation momba?a cultivar was the most productive cultivar but without irrigation no difference was observed among the cultivars. during the dry season (may/01 to august/01), the drymatter yield of irrigated plots increased significantly as the nitrogen levels increased, whereas without irrigation difference between the rates of 50 and 100 kg/ha/cut of n was observed. a positive effect of irrigation was obtained from the final of dry season, as the temperature increased and probably the photoperiod was not a limiting factor. forage management can be anticipated by reducing growth season from august, with dm yield from 1 and 2 t/ha, with n rate of 75 kg/ha/cut, to 3 t/ha, with the highest rate, in relation to the treatment without irrigation.
Volumetric proportions of the goat placenta structural components throughout gestation
SANTOS, Renato de Lima;BARRETO FILHO, Joo Bosco;MARQUES Jr., Ant?nio de Pinho;ANDRADE, Joelmer Santos;
Brazilian Journal of Veterinary Research and Animal Science , 1998, DOI: 10.1590/S1413-95961998000400002
Abstract: the goal of this research was to determine the volumetric proportions of the structural components of the goat placenta throughout gestation. we used nine goats of nondefined breed, separated into groups a, b and c, with 90, 120 and 150 days of gestation, respectively. from each animal fragments of three placentomes were obtained, one removed from the base, another from the middle part and the other from the end of the gestational uterine horn. the fragments were fixed in bouin solution and histologically processed according to the standard techniques and stained with hematoxilin-eosin. microscopically, a 25-point circular grid (zeiss kpl ocular 6.3x with 25 points) was randomly used across the placentome to determine the volumetric proportion of the maternal and fetal connective tissue, syncytium, trophoblastic epithelium and binucleate cells. an increase of volumetric proportion of the syncytium was found at the end of gestation and also a decrease of volumetric proportion of fetal connective tissue in the same period.
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