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Search Results: 1 - 10 of 528 matches for " Jiunn-Jye Sheu "
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Combination of cilostazol and clopidogrel attenuates Rat critical limb ischemia
Sheu Jiunn-Jye,Lin Kun-Chen,Tsai Ching-Yen,Tsai Tzu-Hsien
Journal of Translational Medicine , 2012, DOI: 10.1186/1479-5876-10-164
Abstract: Background and aim Procedural failure and untoward clinical outcomes after surgery remain problematic in critical limb ischemia (CLI) patients. This study tested a clopidogrel-cilostazol combination treatment compared with either treatment alone in attenuating CLI and improving CLI-region blood flow in rats. Methods Male Sprague–Dawley rats (n = 40) were equally divided into five groups: control, CLI induction only, CL I + cilostazol (12.0 mg/day/kg), CLI + clopidogrel (8.0 mg/kg/day) and CLI + combined cilostazol-clopidogrel. After treatment for 21 days, Laser Doppler imaging was performed. Results On day 21, the untreated CLI group had the lowest ratio of ischemic/normal blood flow (p < 0.001). Inflammation measured by VCAM-1 protein expression; oxidative stress; PAI-1, MMP-9 and TNF-α mRNA expressions; and immunofluorescence staining (IF) of CD68+ cells was lower with combined treatment than with the other treatments, and lower in the two single-treatment groups than the untreated CLI group (all p < 0.01). Anti-inflammatory mRNA expression of interleukin-10, and eNOS showed a reverse pattern among these groups. Apoptosis measured by Bax, caspase-3 and PARP; and muscle damage measured by cytosolic cytochrome-C, and serum and muscle micro-RNA-206 were all lowest with combination treatment, and the two single-treatment groups showed lower values than the untreated group (all p < 0.001). Angiogenesis measured by eNOS, IF staining of CD31+ and vWF + cells; and number of vessels in CLI region were highest with combination treatment and higher in the single-treatment groups than the untreated group (all p < 0.001). Conclusion Combined cilostazol-clopidogrel therapy is superior to either agent alone in improving ischemia in rodent CLI.
Diabetes Mellitus Downregulates Expression of Connexin43 in Rat Aortic Medial Smooth Muscle Cells and Can Be Reversed by Simvastatin and Losartan Therapy
Jiunn-Jye Sheu,Tzu-Hsien Tsai,Li-Teh Chang,Chiang-Hua Chiang
Chang Gung Medical Journal , 2008,
Abstract: Background: Diabetes mellitus (DM) plays a crucial role in the pathogenesis of initiationand propagation of atherosclerosis. Although previous studies have suggestedthat interactions between cells form the framework for understanding thepathogenesis of atherosclerosis, little is known about how DM impacts intercellularcommunication within arteries, which occurs via connexin43 (Cx43)gap junctions (GJs). This study tested the hypothesis that DM suppressesexpression of Cx43 GJs, and that this suppression can be abrogated via simvastatinor losartan treatment.Methods: An experimental model of DM (induced by streptozocin 60 mg/kg bodyweight) in adult male rats (n = 24) was utilized to investigate Cx43 expressionin the aorta. These rats were divided into group I (insulin therapy only),group II (insulin plus simvastatin 20 mg/kg/day) and group III (insulin pluslosartan 20 mg/kg/day). Twenty-four diabetic rats and 8 healthy rats (groupIV) were sacrificed 3 weeks after DM induction for Western blot andimmunofluorescence analysis.Results: By day 21, the blood sugar level was significantly higher than the respectivebaseline values in groups I, II and III (all values of p < 0.0001).Additionally, the final blood sugar levels of groups I-III were significantlyhigher than that of group IV (p < 0.0001). The final body weight in group IVwas significantly higher than that in groups I-III (all values of p < 0.0001).Experimental results demonstrated that Cx43 expression in the aortic walldid not differ among groups II-IV (p > 0.1). However, compared with groupsII-IV, Cx43 expression in the aortic wall was significantly mitigated in groupI (all values of p < 0.05). Western blot results showed that relative density ofCx43 to β-actin was significantly higher in groups II-IV than in group I (p <0.01).Conclusions: DM markedly suppressed expression of Cx43 in rat aortic walls. Both simvastatinand losartan treatment significantly reversed the effects of DM onintegrity of Cx43 expression.
Morbidity at elementary school entry differs by sex and level of residence urbanization: a comparative cross-sectional study
Rea-Jeng Yang, Jiunn-Jye Sheu, Huey-Shys Chen, Kuan-Chia Lin, Hsiu-Li Huang
BMC Public Health , 2007, DOI: 10.1186/1471-2458-7-358
Abstract: This is a comparative descriptive study. Data from the 2002 Student Entry Physical Examination (SEPE) and Student Medical History Inventory (SMHI) were obtained from 203 public and private elementary schools in northern Taiwan where a population of 53,053 students was included. Frequencies, independent sample t test, one-way ANOVA along with Scheff's post hoc test, and Pearson's correlation were conducted using SPSS.This study showed that 13.7% of students had at least one diagnosed disease from the SMHI reported by parents. Moreover, the SEPE indicated that 79.5% students had at least one health concern. Dental caries, myopia, and obesity were the most prevalent health problems among the first-graders (69.6%, 27.1%, and 9.5%, respectively). Research results show that there were significant differences in the prevalence of dental caries, myopia, and obesity between different sexes and among levels of urbanization. However, the quantity of available medical resources made no significant difference.Elementary school entry physical examination is an important way to detect students' health problems. It is suggested that school health interventions consider students' health profiles along with their sex and level of urbanization in planning. More research is needed to find the risk factors of the health problems. Additionally, the creation of a school health committee is suggested to implement and evaluate the entry health examination program.Health is vital to a child's ability to learn and to succeed in life [1]. Diseases or health problems can prevent a child from fully engaged in learning activities. For instance, studies found that childhood asthma is associated with an increased risk for school problems, including grade failure and learning disability [2,3]. As a means of primary prevention, many studies suggested that physical examination can identify high risk groups among school-aged children and also provide clues for secondary prevention [4,5]. Physical exami
Extracorporeal Shock Wave Therapy Reverses Ischemia-Related Left Ventricular Dysfunction and Remodeling: Molecular-Cellular and Functional Assessment
Morgan Fu, Cheuk-Kwan Sun, Yu-Chun Lin, Ching-Jen Wang, Chiung-Jen Wu, Sheung-Fat Ko, Sarah Chua, Jiunn-Jye Sheu, Chiang-Hua Chiang, Pei-Lin Shao, Steve Leu, Hon-Kan Yip
PLOS ONE , 2011, DOI: 10.1371/journal.pone.0024342
Abstract: An optimal treatment for patients with diffuse obstructive arterial disease unsuitable for catheter-based or surgical intervention is still pending. This study tested the hypothesis that extracorporeal shock wave (ECSW) therapy may be a therapeutic alternative under such clinical situation. Myocardial ischemia was induced in male mini-pigs through applying an ameroid constrictor over mid-left anterior descending artery (LAD). Twelve mini-pigs were equally randomized into group 1 (Constrictor over LAD only) and group 2 (Constrictor over LAD plus ECSW [800 impulses at 0.09 mJ/mm2] once 3 months after the procedure). Results showed that the parameters measured by echocardiography did not differ between two groups on days 0 and 90. However, echocardiography and left ventricular (LV) angiography showed higher LV ejection fraction and lower LV end-systolic dimension and volume in group 2 on day 180 (p<0.035). Besides, mRNA and protein expressions of CXCR4 and SDF-1α were increased in group 2 (p<0.04). Immunofluorescence staining also showed higher number of vWF-, CD31-, SDF-1α-, and CXCR4-positive cells in group 2 (all p<0.04). Moreover, immunohistochemical staining showed notably higher vessel density but lower mean fibrosis area, number of CD40-positive cells and apoptotic nuclei in group 2 (all p<0.045). Mitochondrial protein expression of oxidative stress was lower, whereas cytochrome-C was higher in group 2 (all p<0.03). Furthermore, mRNA expressions of MMP-9, Bax and caspase-3 were lower, whereas Bcl-2, eNOS, VEGF and PGC-1α were higher in group 2 (all p<0.01). In conclusion, ECSW therapy effectively reversed ischemia-elicited LV dysfunction and remodeling through enhancing angiogenesis and attenuating inflammation and oxidative stress.
Intra-coronary administration of tacrolimus markedly attenuates infarct size and preserves heart function in porcine myocardial infarction
Sarah Chua, Steve Leu, Jiunn-Jye Sheu, Yu-Chun Lin, Li-Teh Chang, Ying-Hsien Kao, Chia-Hung Yen, Tzu-Hsien Tsai, Yung-Lung Chen, Hsueh-Wen Chang, Cheuk-Kwan Sun, Hon-Kan Yip
Journal of Inflammation , 2012, DOI: 10.1186/1476-9255-9-21
Abstract: Twelve male mini-pigs were randomized into AMI-saline (MI-only) group and AMI-tacrolimus (MI-Tac) group that received intra-coronary saline (3.0?mL) and tacrolimus (0.5?mg in 2.5?mL saline) injection, respectively, beyond site of ligation 30 minutes after LAD occlusion.Larger infarct area was noted in MI-only group (p?<?0.001). Inflammatory biomarkers at protein [oxidative stress, tumor necrotic factor-α, nuclear factor-κB], gene (matrix metalloproteinase-9, plasminogen activator inhibitor-1), and cellular (CD40+, CD68+ inflammatory cells) levels were remarkably higher in MI-only animals (p?<?0.01). Conversely, anti-inflammatory biomarkers at gene level (Interleukin-10), gene and protein level (endothelial nitric oxide synthase), and anti-oxidant biomarkers at both gene and protein levels [heme oxygenase 1, NAD(P)H:quinone oxidoreductase] were lower in MI-only group (p?<?0.01). Number of apoptotic nuclei and apoptotic biomarkers expressions at gene and protein levels (Bax, caspase 3) were notably higher, whereas anti-apoptotic biomarkers at gene and protein levels (Bcl-2), LVEF, and fractional shortening were markedly lower in MI-only group (p?<?0.001).Intra-coronary administration of tacrolimus significantly attenuated infarct size and preserved LV function.
Impact of obesity control on circulating level of endothelial progenitor cells and angiogenesis in response to ischemic stimulation
Yung-Lung Chen, Chia-Lo Chang, Cheuk-Kwan Sun, Chiung-Jen Wu, Tzu-Hsien Tsai, Sheng-Ying Chung, Sarah Chua, Kuo-Ho Yeh, Steve Leu, Jiunn-Jye Sheu, Fan-Yen Lee, Chia-Hung Yen, Hon-Kan Yip
Journal of Translational Medicine , 2012, DOI: 10.1186/1479-5876-10-86
Abstract: 8-week-old C57BL/6J mice (n?=?27) were equally divided into group 1 (fed with 22-week control diet), group 2 (22-week high fat diet), and group 3 (14-week high fat diet, followed by 8-week control diet). Critical limb ischemia (CLI) was induced at week 20 in groups 2 and 3. The animals were sacrificed at the end of 22 weeks.Heart weight, body weight, abdominal fat weight, serum total cholesterol level, and fasting blood sugar were highest in group 2 (all p?<?0.001). The numbers of circulating EPCs (C-kit/CD31+, Sca-1/KDR?+?and CXCR4/CD34+) were lower in groups 1 and 2 than in group 3 at 18?h after CLI induction (p?<?0.03). The numbers of differentiated EPCs (C-kit/CD31+, CXCR4/CD34+ and CD133+) from adipose tissue after 14-day cultivation were also lowest in group 2 (p?<?0.001). Protein expressions of VCAM-1, oxidative index, Smad3, and TGF-β were higher, whereas the Smad1/5 and BMP-2, mitochondrial cytochrome-C SDF-1α and CXCR4 were lower in group 2 than in groups 1 and 3 (all p?<?0.02). Immunofluorescent staining of CD31+ and vWF?+?cells, the number of small vessel (<15?μm), and blood flow through Laser Doppler scanning of ischemic area were lower in group 2 compared to groups 1 and 3 on day 14 after CLI induction (all p?<?0.001).Obesity suppressed abilities of angiogenesis and recovery from CLI that were reversed by obesity control.The dramatic increase in the prevalence of obesity is mainly due to living in an environment characterized by calorie-rich foods and lack of physical activity, especially in Western countries [1]. Abundant data have demonstrated that obesity predisposes to a variety of low-grade chronic and systemic inflammatory diseases, including insulin resistance, type 2 diabetes, fatty liver diseases, osteoarthritis, atherosclerosis and its complications [2-7]. Epidemiologic studies have shown that obesity constitutes a major health threat because of its associated morbidity and mortality, especially those from cardiovascular diseases [8-10].Studi
Effects of Extract from Solid-State Fermented Cordyceps sinensis on Type 2 Diabetes Mellitus
Wei-Chih Kan,Hsien-Yi Wang,Chih-Chiang Chien,Shun-Lai Li,Yu-Chun Chen,Liang-Hao Chang,Chia-Hui Cheng,Wan-Chen Tsai,Jyh-Chang Hwang,Shih-Bin Su,Li-Hsueh Huang,Jiunn-Jye Chuu
Evidence-Based Complementary and Alternative Medicine , 2012, DOI: 10.1155/2012/743107
Abstract: Diabetes mellitus is the most common chronic disease in the world, and a wide range of drugs, including Chinese herbs, have been evaluated for the treatment of associated metabolic disorders. This study investigated the potential hypoglycemic and renoprotective effects of an extract from the solid-state fermented mycelium of Cordyceps sinensis (CS). We employed the KK/HIJ diabetic mouse model, in which the mice were provided with a high-fat diet for 8 weeks to induce hyperglycemia, followed by the administration of CS or rosiglitazone for 4 consecutive weeks. Several parameters were evaluated, including changes in body weight, plasma lipid profiles, oral glucose tolerance tests, insulin tolerance tests, and plasma insulin concentrations. Our results show that the CS extract significantly elevated HDL/LDL ratios at 4 weeks and decreased body weight gain at 8 weeks. Interestingly, CS treatment did not lead to obvious improvements in hyperglycemia or resistance to insulin, while in vitro MTT assays indicated that CS protects pancreatic beta cells against the toxic effects of STZ. CS also enhanced renal NKA activity and reduced the accumulation of mesangial matrix and collagen deposition. In conclusion, CS extract can potentially preserve β-cell function and offer renoprotection, which may afford a promising therapy for DM.
Room-temperature super-extraction system(RTSES) optimizes the anxiolytic- andantidepressant-like behavioural effects of traditionalXiao-Yao-San in mice
Shih-Hsi Yin, Ching-Cheng Wang, Tain-Junn Cheng, Chia-Yu Chang, Kao-Chang Lin, Wei-Chih Kan, Hsien-Yi Wang, Wenny Mei-Wen Kao, Yen-Liang Kuo, Jian-Chyi Chen, Shun-Lai Li, Chia-Hui Cheng, Jiunn-Jye Chuu
Chinese Medicine , 2012, DOI: 10.1186/1749-8546-7-24
Abstract: The neuroprotective roles of XYS/RTSES against reserpine-derived neurotoxicity were evaluated using a glial cell injury system (in vitro) and a depression-like C57BL/6 J mouse model (in vivo). The anxiolytic-behavioural effects were measured by the elevated plus-maze (EPM) test and the antidepressant effects were evaluated by the forced swimming test (FST) and tail suspension test (TST). Glucose tolerance and insulin resistance were assayed by ELISA. The expression of 5-HT1A receptors in the prefrontal cortex was examined by western blotting.XYS/RTSES (300 mug/mL) diminished reserpine-induced glial cell death more effectively than either XYS (300 mug/mL) or fluoxetine (30 muM) at 24 h (P = 0.0481 and P = 0.054, respectively). Oral administration of XYS/RTSES (500 mg/kg/day) for 4 consecutive weeks significantly elevated the ratios of entries (open arms/closed arms; P = 0.0177) and shuttle activity (P = 0.00149) on the EPM test, and reduced the immobility time by 90% on the TST (P = 0.00000538) and FST (P = 0.0000053839). XYS/RTSES also improved the regulation of blood glucose (P = 0.0305) and increased the insulin sensitivity (P = 0.0093). The Western blot results indicated that the activation of cerebral 5-HT1A receptors may be involved in the mechanisms of XYS/RTSES actions.The RTSES could provide a novel method for extracting effective anxiolytic- and antidepressant-like substances. XYS/RTSES improved the regulation of blood glucose and increased the insulin sensitivity in reserpine-induced anxiety and depression. Neuroprotection of glial cells and activation of cerebral 5-HT1A receptors were also involved.
Persistent H. pylori colonization in early acquisition age of mice related with higher gastric sialylated Lewis x, IL-10, but lower interferon-γ expressions
Yao-Jong Yang, Hsiao-Bai Yang, Jiunn-Jong Wu, Bor-Shyang Sheu
Journal of Biomedical Science , 2008, DOI: 10.1186/1423-0127-16-34
Abstract: We applied a young (7-day-old) C57BL/6 mice group (n = 50) and adult (6-week-old) C57BL/6 mice group (n = 50). In each group, 30 mice were challenged with H. pylori and 20 mice served as na?ve control. The success of H. pylori colonization was assessed on the 2nd week and the 8th week, respectively. The intensity of the Lewis x, sialylated Lewis x(sialyl-Lex), and cytokine expressions, including TNF-α, IFN-γ, IL-6, IL-10, and IL-1β, were immunochemically stained and graded.On the 2nd week after H. pylori challenge, the colonization rates of H. pylori were similar between the young mice group and the adult mice group (89% vs. 100%, P > 0.05). However, on the 8th week, the H. pylori colonization rate was significantly lower in the young mice group than in the adult mice group (53% vs. 95%, P = 0.003). On the 8th week, the young mice with a persistence of H. pylori colonization had higher sialyl-Lex, higher IL-10, and lower IFN-γ than those of the mice that lost colonization during the 2nd to the 8th week (P < 0.05).The persistence of H. pylori colonization could be an acquisition-age determinant process. After H. pylori exposure at an early acquisition age, the host response with a higher sialyl-Lex and IL-10, but a lower IFN-γ correlates to the consequent persistence of H. pylori colonization.Helicobacter pylori infection can cause chronic gastritis and even peptic ulcer disease in both children and adults [1,2]. In epidemiological studies, the seropositivity of H. pylori has been shown to be less in children than in adults [3,4]. A follow-up cohort study showed that the highest incidence of seroconversion occurs in early childhood and gradually decreases with age [3]. Several studies have suggested that an early acquisition of H. pylori infection in childhood may possibly lead to a subsequent loss of colonization [3-6]. However, the exact regulations within children to determine either loss or persistence of H. pylori colonization after early acquisition remains not
Persistent H. pylori colonization in early acquisition age of mice related with higher gastric sialylated Lewis x, IL-10, but lower interferon-γ expressions
Yang Yao-Jong,Yang Hsiao-Bai,Wu Jiunn-Jong,Sheu Bor-Shyang
Journal of Biomedical Science , 2009,
Abstract: Background H. pylori infection is less prevalent in childhood. This study validated whether the rates of H. pylori colonization depend on different acquisition ages, and correlate with the different gastric Lewis antigens or cytokine expressions after H. pylori acquisition. Methods We applied a young (7-day-old) C57BL/6 mice group (n = 50) and adult (6-week-old) C57BL/6 mice group (n = 50). In each group, 30 mice were challenged with H. pylori and 20 mice served as na ve control. The success of H. pylori colonization was assessed on the 2nd week and the 8th week, respectively. The intensity of the Lewis x, sialylated Lewis x(sialyl-Lex), and cytokine expressions, including TNF-α, IFN-γ, IL-6, IL-10, and IL-1β, were immunochemically stained and graded. Results On the 2nd week after H. pylori challenge, the colonization rates of H. pylori were similar between the young mice group and the adult mice group (89% vs. 100%, P > 0.05). However, on the 8th week, the H. pylori colonization rate was significantly lower in the young mice group than in the adult mice group (53% vs. 95%, P = 0.003). On the 8th week, the young mice with a persistence of H. pylori colonization had higher sialyl-Lex, higher IL-10, and lower IFN-γ than those of the mice that lost colonization during the 2nd to the 8th week (P < 0.05). Conclusion The persistence of H. pylori colonization could be an acquisition-age determinant process. After H. pylori exposure at an early acquisition age, the host response with a higher sialyl-Lex and IL-10, but a lower IFN-γ correlates to the consequent persistence of H. pylori colonization.
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