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Search Results: 1 - 10 of 80829 matches for " Jingran Liu "
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Diagnosis of Premature Senescence of Cotton Using SPAD Value  [PDF]
Pengcheng Li, Helin Dong, Aizhong Liu, Jingran Liu, Miao Sun, Guoping Wang, Siping Zhang, Yabing Li, Shuchun Mao
Agricultural Sciences (AS) , 2014, DOI: 10.4236/as.2014.511107
Abstract: Field experiments were conducted in 2011 on an experimental farm at the Cotton Research Insti-tute, Chinese Academy of Agricultural Sciences, Anyang, China. We conducted experiments with a “SPAD-502” meter to quickly measure the relative value of chlorophyll content in the leaf blades of field cotton. Our goal was to seek a suitable leaf on a cotton plant to diagnose senescence status of crop plants at later stages of growth. We began by studying the dynamic change rule of the dis-tal-most four leaves of the cotton plant during the entire growth period with two early-maturing cultivars (CCRI 36, CCRI 50) and two mid-maturing cultivars (CCRI 41, SCRC 28). We also examined the effect of different nitrogen and potassium fertilizer rates on SPAD values of the leaves of SCRC 28. Our results suggest that SPAD values of the 1st distal stem leaves from two early cultivars can act as good indicators of senescence status in the plants, if they are measured between 10 d before the boll-opening stage and 10 d after boll opening stage. Differences of SPAD values of the 3rd distal stem leaves of two mid-maturing cultivars measured between about 15 d before the boll opening stage and 15 d after the boll opening stage can also be used to measure senescence status in these cotton cultivars. The conclusion can be used for cotton producer to manage N fertilizer better at later growth stage.
Prediction of multiple drug resistance phenotype in cancer cell lines using gene expression profiles and phylogenetic trees
XinYi Liu,ZuoFeng Li,JingRan Wen,QingQing Cai,Ye Xu,XiaoYan Zhang
Chinese Science Bulletin , 2010, DOI: 10.1007/s11434-010-4131-2
Abstract: When microarray gene expression data are used to predict multiple drug resistance (MDR) phenotypes for anticancer drugs, the normalization strategy and the quality of the selected signature genes are usually the main causes of inconsistency among different experiments. A stable statistical drug response prediction model is urgently required in oncology. In this study, the microarray gene expression data of multiple cancer cell lines with MDR was analyzed. For each probe-set, the expression value was defined as present/absent (1/0) and was classified into a gene set defined with protein domain organization (PDO). After employing the gene content method of phylogenetic analysis, a phylogenetic model (cell tree) for MDR phenotype prediction was built at the PDO gene set level. The results indicate that classification of cancer cell lines is predominantly affected by both the histopathological features and the MDR phenotype (paclitaxel and vinblastine). When applying this model to predict the MDR phenotype of independent samples, the phylogenetic model performs better than signature gene models. Although the utility of our procedure is limited due to sample heterogeneity, it still has potential application in MDR research, especially for hematological tumors or established cell lines.
The Effects of Fruiting Positions on Cellulose Synthesis and Sucrose Metabolism during Cotton (Gossypium hirsutum L.) Fiber Development
Yina Ma, Youhua Wang, Jingran Liu, Fengjuan Lv, Ji Chen, Zhiguo Zhou
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0089476
Abstract: Cotton (Gossypium hirsutum L.) boll positions on a fruiting branch vary in their contribution to yield and fiber quality. Fiber properties are dependent on deposition of cellulose in the fiber cell wall, but information about the enzymatic differences in sucrose metabolism between these fruiting positions is lacking. Therefore, two cotton cultivars with different sensitivities to low temperature were tested in 2010 and 2011 to quantify the effect of fruit positions (FPs) on fiber quality in relation to sucrose content, enzymatic activities and sucrose metabolism. The indices including sucrose content, sucrose transformation rate, cellulose content, and the activities of the key enzymes, sucrose phosphate synthase (SPS), acid invertase (AI) and sucrose synthase (SuSy) which inhibit cellulose synthesis and eventually affect fiber quality traits in cotton fiber, were determined. Results showed that as compared with those of FP1, cellulose content, sucrose content, and sucrose transformation rate of FP3 were all decreased, and the variations of cellulose content and sucrose transformation rate caused by FPs in Sumian 15 were larger than those in Kemian 1. Under FP effect, activities of SPS and AI in sucrose regulation were decreased, while SuSy activity in sucrose degradation was increased. The changes in activities of SuSy and SPS in response to FP effect displayed different and large change ranges between the two cultivars. These results indicate that restrained cellulose synthesis and sucrose metabolism in distal FPs are mainly attributed to the changes in the activities of these enzymes. The difference in fiber quality, cellulose synthesis and sucrose metabolism in response to FPs in fiber cells for the two cotton cultivars was mainly determined by the activities of both SuSy and SPS.
Monoclonal Antibodies against Accumulation-Associated Protein Affect EPS Biosynthesis and Enhance Bacterial Accumulation of Staphylococcus epidermidis
Jian Hu,Tao Xu,Tao Zhu,Qiang Lou,Xueqin Wang,Yang Wu,Renzheng Huang,Jingran Liu,Huayong Liu,Fangyou Yu,Baixing Ding,Yalin Huang,Wenyan Tong,Di Qu
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0020918
Abstract: Because there is no effective antibiotic to eradicate Staphylococcus epidermidis biofilm infections that lead to the failure of medical device implantations, the development of anti-biofilm vaccines is necessary. Biofilm formation by S. epidermidis requires accumulation-associated protein (Aap) that contains sequence repeats known as G5 domains, which are responsible for the Zn2+-dependent dimerization of Aap to mediate intercellular adhesion. Antibodies against Aap have been reported to inhibit biofilm accumulation. In the present study, three monoclonal antibodies (MAbs) against the Aap C-terminal single B-repeat construct followed by the 79-aa half repeat (AapBrpt1.5) were generated. MAb18B6 inhibited biofilm formation by S. epidermidis RP62A to 60% of the maximum, while MAb25C11 and MAb20B9 enhanced biofilm accumulation. All three MAbs aggregated the planktonic bacteria to form visible cell clusters. Epitope mapping revealed that the epitope of MAb18B6, which recognizes an identical area within AapBrpt constructs from S. epidermidis RP62A, was not shared by MAb25C11 and MAb20B9. Furthermore, all three MAbs were found to affect both Aap expression and extracellular polymeric substance (EPS, including extracellular DNA and PIA) biosynthesis in S. epidermidis and enhance the cell accumulation. These findings contribute to a better understanding of staphylococcal biofilm formation and will help to develop epitope-peptide vaccines against staphylococcal infections.
Refined Asymptotics of the Finite-Size Magnetization via a New Conditional Limit Theorem for the Spin
Richard S. Ellis,Jingran Li
Mathematics , 2012,
Abstract: We study the fluctuations of the spin per site around the thermodynamic magnetization in the mean-field Blume-Capel model. Our main theorem generalizes the main result in a previous paper (Ellis, Machta, and Otto) in which the first rigorous confirmation of the statistical mechanical theory of finite-size scaling for a mean-field model is given. In that paper our goal is to determine whether the thermodynamic magnetization is a physically relevant estimator of the finite-size magnetization. This is done by comparing the asymptotic behaviors of these two quantities along parameter sequences converging to either a second-order point or the tricritical point in the mean-field Blume-Capel model. The main result is that the thermodynamic magnetization and the finite-size magnetization are asymptotic when the parameter $\alpha$ governing the speed at which the sequence approaches criticality is below a certain threshold $\alpha_0$. Our main theorem in the present paper on the fluctuations of the spin per site around the thermodynamic magnetization is based on a new conditional limit theorem for the spin, which is closely related to a new conditional central limit theorem for the spin.
The Two-Component Signal Transduction System ArlRS Regulates Staphylococcus epidermidis Biofilm Formation in an ica-Dependent Manner
Yang Wu, Jiaxue Wang, Tao Xu, Jingran Liu, Wenqi Yu, Qiang Lou, Tao Zhu, Nianan He, Haijing Ben, Jian Hu, Friedrich G?tz, Di Qu
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0040041
Abstract: Due to its ability to form biofilms on medical devices, Staphylococcus epidermidis has emerged as a major pathogen of nosocomial infections. In this study, we investigated the role of the two-component signal transduction system ArlRS in regulating S. epidermidis biofilm formation. An ArlRS-deficient mutant, WW06, was constructed using S. epidermidis strain 1457 as a parental strain. Although the growth curve of WW06 was similar to that of SE1457, the mutant strain was unable to form biofilms in vitro. In a rabbit subcutaneous infection model, sterile disks made of polymeric materials were implanted subcutaneously followed with inoculation of WW06 or SE1457. The viable bacteria cells of WW06 recovered from biofilms on the embedded disks were much lower than that of SE1457. Complementation of arlRS genes expression from plasmid in WW06 restored biofilm-forming phenotype both in vivo and in vitro. WW06 maintained the ability to undergo initial attachment. Transcription levels of several genes involved in biofilm formation, including icaADBC, sigB, and sarA, were decreased in WW06, compared to SE1457; and icaR expression was increased in WW06, detected by real-time reverse-transcription PCR. The biofilm-forming phenotype was restored by overexpressing icaADBC in WW06 but not by overexpressing sigB, indicating that ArlRS regulates biofilm formation through the regulation of icaADBC. Gel shift assay showed that ArlR can bind to the promoter region of the ica operon. In conclusion, ArlRS regulates S. epidermidis biofilm formation in an ica-dependent manner, distinct from its role in S. aureus.
Role of the SaeRS two-component regulatory system in Staphylococcus epidermidis autolysis and biofilm formation
Qiang Lou, Tao Zhu, Jian Hu, Haijing Ben, Jinsong Yang, Fangyou Yu, Jingran Liu, Yang Wu, Adrien Fischer, Patrice Francois, Jacques Schrenzel, Di Qu
BMC Microbiology , 2011, DOI: 10.1186/1471-2180-11-146
Abstract: The saeRS genes of SE1457 were deleted by homologous recombination. The saeRS deletion mutant, SE1457ΔsaeRS, exhibited increased biofilm formation that was disturbed more severely (a 4-fold reduction) by DNase I treatment compared to SE1457 and the complementation strain SE1457saec. Compared to SE1457 and SE1457saec, SE1457ΔsaeRS showed increased Triton X-100-induced autolysis (approximately 3-fold) and decreased cell viability in planktonic/biofilm states; further, SE1457ΔsaeRS also released more extracellular DNA (eDNA) in the biofilms. Correlated with the increased autolysis phenotype, the transcription of autolysis-related genes, such as atlE and aae, was increased in SE1457ΔsaeRS. Whereas the expression of accumulation-associated protein was up-regulated by 1.8-fold in 1457ΔsaeRS, the expression of an N-acetylglucosaminyl transferase enzyme (encoded by icaA) critical for polysaccharide intercellular adhesin (PIA) synthesis was not affected by the deletion of saeRS.Deletion of saeRS in S. epidermidis resulted in an increase in biofilm-forming ability, which was associated with increased eDNA release and up-regulated Aap expression. The increased eDNA release from SE1457ΔsaeRS was associated with increased bacterial autolysis and decreased bacterial cell viability in the planktonic/biofilm states.The opportunistic pathogen Staphylococcus epidermidis has emerged as an important etiologic agent of nosocomial infections. The ability to form biofilms on the surfaces of medical devices is an important component of S. epidermidis pathogenicity. Biofilm resistance to antibiotics and host defense mechanisms are often regulated by two-component signal transduction systems (TCSs) [1].Biofilm formation proceeds in two distinct developmental phases: primary attachment of staphylococcal cells to a polystyrene surface followed by bacterial accumulation in multiple layers [2]. The initial adhesion of bacterial cells to a polymer surface is influenced by a variety of factors, in
Accurate assessment of antibiotic susceptibility and screening resistant strains of a bacterial population by linear gradient plate
YuQing Liu,JingRan Li,JiaFa Du,Ming Hu,Hua Bai,Jing Qi,Chao Gao,TianTian Wei,Hong Su,JianLing Jin,PeiJi Gao
Science China Life Sciences , 2011, DOI: 10.1007/s11427-011-4230-6
Abstract: The dynamics of a bacterial population exposed to the minimum inhibitory concentration (MIC) of an antibiotic is an important issue in pharmacological research. Therefore, a novel antibiotic susceptibility test is urgently needed that can both precisely determine the MIC and accurately select antibiotic-resistant strains from clinical bacterial populations. For this purpose, we developed a method based on Fick’s laws of diffusion using agar plates containing a linear gradient of antibiotic. The gradient plate contained two layers. The bottom layer consisted of 15 mL agar containing the appropriate concentration of enrofloxacin and allowed to harden in the form of a wedge with the plate slanted such that the entire bottom was just covered. The upper layer consisted of 15 mL plain nutrient agar added with the plate held in the horizontal position. After allowing vertical diffusion of the drug from the bottom agar layer for 12 h, the enrofloxacin concentration was diluted in proportion to the ratio of the agar layer thicknesses. The uniform linear concentration gradient was verified by measuring the enrofloxacin concentration on the agar surface. When heavy bacterial suspensions were spread on the agar surface and incubated for more than 12 h, only resistant cells were able to form colonies beyond the boundary of confluent growth of susceptible cells. In this way, the true MIC of enrofloxacin was determined. The MICs obtained using this linear gradient plate were consistent with those obtained using conventional antibiotic susceptibility tests. Discrete colonies were then spread onto a gradient plate with higher antibiotic concentrations; the boundary line increased significantly, and gene mutations conferring resistance were identified. This new method enables the rapid identification of resistant strains in the bacterial population. Use of the linear gradient plate can easily identify the precise MIC and reveal the dynamic differentiation of bacteria near the MIC. This method allows the study of genetic and physiological characteristics of individual strains, and may be useful for early warning of antibiotic resistance that may occur after use of certain antimicrobial agents, and guide clinical treatment.
Comparison of Reference Number of 6 Academic Journals Published in China and Outside China

LI JingRan,WEI LiHui,

中国科技期刊研究 , 2011,
Abstract: 本文对2007年至2009年出版的国内外6种妇产科学术期刊发表的论著和短篇论著2858篇论文的引文数量、分布特征进行比较。结果显示,国内3种妇产科期刊引文数为12605篇,篇均引文数为8.3篇。国外3种妇产科期刊引文数为34663篇,篇均引文数为25.8篇。国内3种妇产科期刊引文在引言、方法和讨论中的引文数分别占16.8%,8.9%和74.3%;国外3种妇产科期刊分别是47.0%,11.2%和41.8%。国内外论文引言和讨论部分引文数量比较,差异有统计学意义(P<0.05),提示3种国内妇产科期刊与国外妇产科期刊的论文引文数量和分布特征明显不同。
Growth of alumina oxide nanowires in an aluminum anodization process
KaiGui Zhu,JingRan Cheng,MingChao Chang,Wu Wang,WenBo Wei,GuangLu Ge
Chinese Science Bulletin , 2011, DOI: 10.1007/s11434-011-4507-y
Abstract: Alumina oxide nanowires were grown via a two-step anodization of aluminum foil. A growth mechanism is proposed for these nanowires which is different from that of those formed using chemical etching methods. An electric field and stress are necessary to grow these nanowires, and the nano-imprinted metal surface of the aluminum foil has an important role.
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