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Search Results: 1 - 10 of 120234 matches for " Jingjie Wang "
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Molecular and cellular evidence for biased mitotic gene conversion in hybrid scallop
Shi Wang, Lingling Zhang, Jingjie Hu, Zhenmin Bao, Zhanjiang Liu
BMC Evolutionary Biology , 2010, DOI: 10.1186/1471-2148-10-6
Abstract: Through the use of a hybrid scallop system (Chlamys farreri ♀ × Argopecten irradians ♂), here we provide solid molecular and cellular evidence for homogenization of the rDNA sequences into maternal genotypes. The ITS regions of the rDNA of the two scallop species exhibit distinct sequences and thereby restriction fragment length polymorphism (RFLP) patterns, and such a difference was exploited to follow the parental ITS contributions in the F1 hybrid during early development using PCR-RFLP. The representation of the paternal ITS decreased gradually in the hybrid during the development of the hybrid, and almost diminished at the 14th day after fertilization while the representation of the maternal ITS gradually increased. Chromosomal-specific fluorescence in situ hybridization (FISH) analysis in the hybrid revealed the presence of maternal ITS sequences on the paternal ITS-bearing chromosomes, but not vice versa. Sequence analysis of the ITS region in the hybrid not only confirmed the maternally biased conversion, but also allowed the detection of six recombinant variants in the hybrid involving short recombination regions, suggesting that site-specific recombination may be involved in the maternally biased gene conversion.Taken together, these molecular and cellular evidences support rapid concerted gene evolution via maternally biased gene conversion. As such a process would lead to the expression of only one parental genotype, and have the opportunities to generate recombinant intermediates; this work may also have implications in novel hybrid zone alleles and genetic imprinting, as well as in concerted gene evolution. In the course of evolution, many species may have evolved involving some levels of hybridization, intra- or interspecific, the sex-biased sequence homogenization could have led to a greater role of one sex than the other in some species.Concerted evolution is the tendency of the different genes in a gene family or cluster to evolve in concert, resul
Promote the Industry Standard of Smart Home in China by Intelligent Router Technology
Hui Lin,Jianbiao Lin,Ke Ji,Jingjie Wang,Feng Lin
Computer Science , 2015,
Abstract: The reason why smart home remains not popularized lies in bad product user experience, purchasing cost, and compatibility, and a lack of industry standard[1]. Echoing problems above, and having relentless devoted to software and hardware innovation and practice, we have independently developed a set of solution which is based on innovation and integration of router technology, mobile Internet technology,Internet of things technology,communication technology, digital-to-analog conversion and codec technology, and P2P technology among others. We have also established relevant protocols (without the application of protocols abroad). By doing this, we managed to establish a system with low and moderate price, superior performance, all-inclusive functions, easy installation, convenient portability, real-time reliability, security encryption, and the capability to manage home furnitures in an intelligent way. Only a new smart home system like this can inject new idea and energy into smart home industry and thus vigorously promote the establishment of smart home industry standard.
Reliability and validity of the Chinese version of the Psoriasis Disability Index (PDI) in Chinese patients with psoriasis
Zehui He, Chuanjian Lu, Aihua Ou, Jiqian Fang, Dongmei Wang, Jingwen Deng, Zhongzhao Zhang, Jingjie Yu
Health and Quality of Life Outcomes , 2012, DOI: 10.1186/1477-7525-10-37
Abstract: A multi-center, cross-sectional study was conducted at 9 hospitals including patients aged 18?years and over. Reliability was determined by internal consistency using Cronbach’s alpha. Validity was assessed through convergent validity and known groups validity. Dimensionality of the PDI was examined by exploratory factor analysis in working patients and nonworking patients respectively.In all, 831 patients were studied. Internal consistency of the PDI was satisfactory. Cronbach’s alpha coefficient was 0.91 for the total score and over 0.70 for each subscale of the PDI. Evidence of convergent validity of the PDI was proved by excellent and moderate to good correlations with the Dermatology Life Quality Index (DLQI) and four subscales of the Short Form-36 (SF-36) (role-physical, bodily pain, social functioning, and role-emotional): r?=?0.51-0.78. Known groups validity was confirmed that the PDI score discriminated well among patients with different severity of psoriasis. The dimensionality of the PDI was determined by the presence of two-factor structure for working patients and three-factor structure for nonworking patients which accounted for 57.3% and 62.3% of the variance respectively.The Chinese version of the PDI is a reliable and valid instrument to assess the impact of psoriasis on patients’ lives and could be used in future quality of life assessment of Chinese patients with psoriasis.
Clinical and radiographic outcomes of the treatment of adolescent idiopathic scoliosis with segmental pedicle screws and combined local autograft and allograft bone for spinal fusion: a retrospective case series
Xiaoming Yang, Hongguang Xu, Ming Li, Suxi Gu, Xiutong Fang, Jingjie Wang, Jianqiang Ni, Dajiang Wu
BMC Musculoskeletal Disorders , 2010, DOI: 10.1186/1471-2474-11-159
Abstract: A combination of in situ local autograft bone and freeze-dried cancellous allograft blocks were used in 50 consecutive patients with adolescent idiopathic scoliosis treated by posterior fusion and Moss Miami pedicle screw instrumentation. Results were assessed clinically and radiographically and quality of life and functional outcome was evaluated by administration of a Chinese version of the SRS-22 survey.There were 41 female and 9 male patients included for analysis with an average age of 14.7 years (range, 12-17). All patients had a minimum follow-up of 18 months (range, 18 to 40 months). The average preoperative Cobb angle was 49.8° (range, 40° to 86°). The average number of levels fused was 9.8 (range, 6-15). Patients had a minimum postoperative follow-up of 18 months. At final follow-up, the average Cobb angle correction was 77.8% (range, 43.4 to 92.5%). There was no obvious loss in the correction, and the average loss of correction was 1.1° (range, 0° to 4°). There was no pseudarthrosis and no major complications.In situ autograft bone combined with allograft bone may be a promising method enhances spinal fusion in AIS treated with pedicle screw placement. By eliminating the need for iliac crest bone harvesting, significant morbidity may be avoided.The iliac crest is considered the best source of autograft bone for procedures treating bone nonunion, spinal fusion, and specifically posterior spinal fusion in corrective surgery for adolescent idiopathic scoliosis (AIS). However, studies have reported numerous complications associated with harvesting iliac crest bone including bleeding, infection, gait disturbance, neurological injury, fracture, and persistent donor site pain, with an incidence ranging from 24% to 29% [1-3]. The high morbidity of iliac crest bone graft harvesting has limited its application. Allograft bone has the advantages of adequate supply and variety of type, and reports indicate that allograft bone is a suitable alternative to autogenous b
One-pot synthesis of magnetic, macro/mesoporous bioactive glasses for bone tissue engineering
Dan Wang, Huiming Lin, Jingjie Jiang, Xiao Han, Wei Guo, Xiaodan Wu, Yingxue Jin and Fengyu Qu
Science and Technology of Advanced Materials , 2013, DOI: 10.1088/1468-6996/14/2/025004
Abstract: Magnetic and macro/mesoporous bioactive glasses were synthesized by a one-pot method via a handy salt leaching technique. It was identified to be an effective and simple synthetic strategy. The non-ionic triblock copolymer, poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol) (P123), was used as the structure directing agent for mesoporous structure but also as the reductant to reduce the iron source into magnetic iron oxide. The prepared materials exhibited excellent super-paramagnetic property with interconnected macroporous (200–300 μm) and mesoporous (3.4 nm) structure. Furthermore, their outstanding drug storage/release properties and rapid (5) induction of hydroxyapatite growth ability were investigated after immersing in simulated body fluid solution at 37 °C. Notably, the biocompatibility assessment confirmed that the materials obtained presented good biocompatibility and enhanced adherence of HeLa cells. Herein, the novel materials are expected to have potential application for bone tissue engineering.
Transcriptome Sequencing and De Novo Analysis for Yesso Scallop (Patinopecten yessoensis) Using 454 GS FLX
Rui Hou, Zhenmin Bao, Shan Wang, Hailin Su, Yan Li, Huixia Du, Jingjie Hu, Shi Wang, Xiaoli Hu
PLOS ONE , 2011, DOI: 10.1371/journal.pone.0021560
Abstract: Background Bivalves comprise 30,000 extant species, constituting the second largest group of mollusks. However, limited genetic research has focused on this group of animals so far, which is, in part, due to the lack of genomic resources. The advent of high-throughput sequencing technologies enables generation of genomic resources in a short time and at a minimal cost, and therefore provides a turning point for bivalve research. In the present study, we performed de novo transcriptome sequencing to first produce a comprehensive expressed sequence tag (EST) dataset for the Yesso scallop (Patinopecten yessoensis). Results In a single 454 sequencing run, 805,330 reads were produced and then assembled into 32,590 contigs, with about six-fold sequencing coverage. A total of 25,237 unique protein-coding genes were identified from a variety of developmental stages and adult tissues based on sequence similarities with known proteins. As determined by GO annotation and KEGG pathway mapping, functional annotation of the unigenes recovered diverse biological functions and processes. Transcripts putatively involved in growth, reproduction and stress/immune-response were identified. More than 49,000 single nucleotide polymorphisms (SNPs) and 2,700 simple sequence repeats (SSRs) were also detected. Conclusion Our data provide the most comprehensive transcriptomic resource currently available for P. yessoensis. Candidate genes potentially involved in growth, reproduction, and stress/immunity-response were identified, and are worthy of further investigation. A large number of SNPs and SSRs were also identified and ready for marker development. This resource should lay an important foundation for future genetic or genomic studies on this species.
Production of Single-Cell Protein from Hydrolysate of Rice Hull in External-loop Airlift Bioreactors
利用稻谷壳水解液在气升式生物反应器中发酵生产单细胞蛋白

Zheng Yuguo,Chen Xiaolong,Wang Zhao,Qian Junqing,Qian Jingjie,
郑裕国
,陈小龙,汪钊,钱俊青,吕一峰,钱静杰,Zheng Yuguo,Chen Xiaolong,Wang Zhao,Qian Junqing,Lü Yifeng,Qian Jingjie

环境科学 , 2000,
Abstract: 以稻谷壳水解液为原料 ,采用树状假丝酵母 AS1.257( Candida arborea AS1.257) ,在罐体高径比为 2.9和上升管与下降管的直径之比为 6.6的外循环气升式生物反应器中发酵生产单细胞蛋白 .研究了通风量、装料量、空气喷嘴直径等工艺和结构参数对发酵的影响 ,得到了较佳的发酵操作参数 .在发酵前 24h的通风量为 1.1m3/( m3· min) ,后 24 h的通风量为 1.4m3/( m3· min)~ 1.5m3/( m3· min) ,起始装料量为 8.5L~ 9.0 L,起始 pH为 4.5~ 5.0 ,发酵温度为 29± 1℃的操作条件下 ,经 48h的发酵 ,可得到较佳的结果 .在无筛板 ,带一块筛板和带二块筛板的外循环气升式生物反应器中 ,发酵 48h后的干基粗蛋白含量分别为 61.3%、62.9%、64.5% ,发酵液中干物质重分别为 20.8mg/ml、21.2 mg/ml、21.3mg/ml,总糖利用率分别为 78.2 %、83.9%、81.4% ,均高于机械搅拌生物反应器 ,气升式生物反应器内加装筛板可提高发酵得率 .
Methods and Countermeasures of Controlling Drilling Cost under New Normal  [PDF]
Haixu Qu, He Huang, Haiqing Cui, Jingjie Liu, Liang Zhao
Energy and Power Engineering (EPE) , 2015, DOI: 10.4236/epe.2015.713055
Abstract: The oil price drops in the international crude oil market and the benefit of upstream operations reduces which has been the new normal of oil companies so far. The upstream operations production and management of oil companies face numerous problems and challenges. One of the most significant aspects is to control and optimize investment. Drilling investment is an important component of development investment in drilling oil and gas wells. This paper proposes ways and countermeasures of controlling drilling cost from 3 aspects which are technology innovation, management innovation and system innovation on basis of studying drilling cost structure and influence factors analysis. The results have some guidance and exemplary significance to show how to improve production and management effect respectively on upstream operations of oil companies under new normal.
Transcriptome Sequencing and Characterization for the Sea Cucumber Apostichopus japonicus (Selenka, 1867)
Huixia Du, Zhenmin Bao, Rui Hou, Shan Wang, Hailin Su, Jingjing Yan, Meilin Tian, Yan Li, Wen Wei, Wei Lu, Xiaoli Hu, Shi Wang, Jingjie Hu
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0033311
Abstract: Background Sea cucumbers are a special group of marine invertebrates. They occupy a taxonomic position that is believed to be important for understanding the origin and evolution of deuterostomes. Some of them such as Apostichopus japonicus represent commercially important aquaculture species in Asian countries. Many efforts have been devoted to increasing the number of expressed sequence tags (ESTs) for A. japonicus, but a comprehensive characterization of its transcriptome remains lacking. Here, we performed the large-scale transcriptome profiling and characterization by pyrosequencing diverse cDNA libraries from A. japonicus. Results In total, 1,061,078 reads were obtained by 454 sequencing of eight cDNA libraries representing different developmental stages and adult tissues in A. japonicus. These reads were assembled into 29,666 isotigs, which were further clustered into 21,071 isogroups. Nearly 40% of the isogroups showed significant matches to known proteins based on sequence similarity. Gene ontology (GO) and KEGG pathway analyses recovered diverse biological functions and processes. Candidate genes that were potentially involved in aestivation were identified. Transcriptome comparison with the sea urchin Strongylocentrotus purpuratus revealed similar patterns of GO term representation. In addition, 4,882 putative orthologous genes were identified, of which 202 were not present in the non-echinoderm organisms. More than 700 simple sequence repeats (SSRs) and 54,000 single nucleotide polymorphisms (SNPs) were detected in the A. japonicus transcriptome. Conclusion Pyrosequencing was proven to be efficient in rapidly identifying a large set of genes for the sea cucumber A. japonicus. Through the large-scale transcriptome sequencing as well as public EST data integration, we performed a comprehensive characterization of the A. japonicus transcriptome and identified candidate aestivation-related genes. A large number of potential genetic markers were also identified from the A. japonicus transcriptome. This transcriptome resource would lay an important foundation for future genetic or genomic studies on this species.
Identification of Reference Genes for qRT-PCR Analysis in Yesso Scallop Patinopecten yessoensis
Liying Feng, Qian Yu, Xue Li, Xianhui Ning, Jing Wang, Jiajun Zou, Lingling Zhang, Shi Wang, Jingjie Hu, Xiaoli Hu, Zhenmin Bao
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0075609
Abstract: Background Bivalves comprise around 30,000 extant species and have received much attention for their importance in ecosystems, aquaculture and evolutionary studies. Despite the increasing application of real-time quantitative reverse transcription PCR (qRT-PCR) in gene expression studies on bivalve species, little research has been conducted on reference gene selection which is critical for reliable and accurate qRT-PCR analysis. For scallops, systematic evaluation of reference genes that can be used among tissues or embryo/larva stages is lacking, and β-actin (ACT) is most frequently used as qRT-PCR reference gene without validation. Results In this study, 12 commonly used candidate reference genes were selected from the transcriptome data of Yesso scallop (Patinopecten yessoensis) for suitable qRT-PCR reference genes identification. The expression of these genes in 36 tissue samples and 15 embryo/larva samples under normal physiological conditions was examined by qRT-PCR, and their expression stabilities were evaluated using three statistic algorithms, geNorm, NormFinder, and comparative ?Ct method. Similar results were obtained by the three approaches for the most and the least stably expressed genes. Final comprehensive ranking for the 12 genes combing the results from the three programs showed that, for different tissues, DEAD-box RNA helicase (HELI), ubiquitin (UBQ), and 60S ribosomal protein L16 (RPL16) were the optimal reference genes combination, while for different embryo/larva stages, gene set containing Cytochrome B (CB), Cytochrome C (CC), Histone H3.3 (His3.3), and Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were recommended for qRT-PCR normalization. ACT was among the least stable genes for both adult tissues and embryos/larvae. Conclusions This work constitutes the first systematic analysis on reference genes selection for qRT-PCR normalization in scallop under normal conditions. The suitable reference genes we recommended will be useful for the identification of genes related to biological processes in Yesso scallop, and also in the reference gene selection for other scallop or bivalve species.
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