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Search Results: 1 - 10 of 8009 matches for " Jim Swildens "
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Human Embryonic and Fetal Mesenchymal Stem Cells Differentiate toward Three Different Cardiac Lineages in Contrast to Their Adult Counterparts
Arti A. Ramkisoensing, Dani?l A. Pijnappels, Sa?d F. A. Askar, Robert Passier, Jim Swildens, Marie José Goumans, Cindy I. Schutte, Antoine A. F. de Vries, Sicco Scherjon, Christine L. Mummery, Martin J. Schalij, Douwe E. Atsma
PLOS ONE , 2011, DOI: 10.1371/journal.pone.0024164
Abstract: Mesenchymal stem cells (MSCs) show unexplained differences in differentiation potential. In this study, differentiation of human (h) MSCs derived from embryonic, fetal and adult sources toward cardiomyocytes, endothelial and smooth muscle cells was investigated. Labeled hMSCs derived from embryonic stem cells (hESC-MSCs), fetal umbilical cord, bone marrow, amniotic membrane and adult bone marrow and adipose tissue were co-cultured with neonatal rat cardiomyocytes (nrCMCs) or cardiac fibroblasts (nrCFBs) for 10 days, and also cultured under angiogenic conditions. Cardiomyogenesis was assessed by human-specific immunocytological analysis, whole-cell current-clamp recordings, human-specific qRT-PCR and optical mapping. After co-culture with nrCMCs, significantly more hESC-MSCs than fetal hMSCs stained positive for α-actinin, whereas adult hMSCs stained negative. Furthermore, functional cardiomyogenic differentiation, based on action potential recordings, was shown to occur, but not in adult hMSCs. Of all sources, hESC-MSCs expressed most cardiac-specific genes. hESC-MSCs and fetal hMSCs contained significantly higher basal levels of connexin43 than adult hMSCs and co-culture with nrCMCs increased expression. After co-culture with nrCFBs, hESC-MSCs and fetal hMSCs did not express α-actinin and connexin43 expression was decreased. Conduction velocity (CV) in co-cultures of nrCMCs and hESC-MSCs was significantly higher than in co-cultures with fetal or adult hMSCs. In angiogenesis bioassays, only hESC-MSCs and fetal hMSCs were able to form capillary-like structures, which stained for smooth muscle and endothelial cell markers.Human embryonic and fetal MSCs differentiate toward three different cardiac lineages, in contrast to adult MSCs. Cardiomyogenesis is determined by stimuli from the cellular microenvironment, where connexin43 may play an important role.
The Impact of a Drug Safety Warning on Discussions between Doctors and Their Patients; the Case of Rosiglitazone  [PDF]
Jim Nuovo
Pharmacology & Pharmacy (PP) , 2011, DOI: 10.4236/pp.2011.23024
Abstract: The goal of this study was to track the influence of a highly publicized report on discussions between doctors and their patients and prescribing decisions made in response to concerns about potential medication adverse side effects. This was a retrospective analysis of a primary care network’s electronic medical record database. From a diabetes registry of 12, 246 patients, 329 were identified as taking rosiglitazone prior to the June 14, 2007 release of an article in the New England Journal of Medicine; the article suggesting an increased risk of myocardial events. The entire content of all office visits, telephone messages, and medication lists for each patient were reviewed over a 2-year period subsequent to the article’s publication. Doctor/patient discussions regarding concerns for rosiglitazone were catalogued including the physician’s treatment recommendations. There were documented discussions on rosiglitazone’s potential adverse side effects for 64 patients; 19.5 percent of this population. All of the discussions occurred between June 15 and October 30, 2007. Of the entire group, 59.3 percent (N = 195) remained on rosiglitazone. For those advised to continue rosiglitazone, the provider indicated that he/she wanted more data before determining if the drug was not safe or discounted the validity of the safety concerns. For those advised to discontinue rosiglitazone, 112 (83.6 percent) were placed on pioglitazone. An article suggesting potential adverse effects of rosiglitazone resulted in a documented discussion in 19.5 percent of patients on this medication. These findings suggest an awareness of this publication by patients, presumably derived from media reports. However, an awareness of this concern did not result in a substantial change in practice.The majority of patients remained on rosiglitazone. The content of these discussions suggest that most physicians’ recommended waiting for more published data before considering a change. While many factors influence physician’s prescribing behavior, this study demonstrates how a highly publicized report influences the doctor/ patient dialogue.
Organic Wastes to Increase CO2 Absorption  [PDF]
Manuel Jiménez Aguilar
International Journal of Clean Coal and Energy (IJCCE) , 2014, DOI: 10.4236/ijcce.2014.34005
Abstract: The objective of the study was actually the investigation of the effect of various organic wastes on the ability of urine in absorbing CO2. Urine alone or mixed with olive-oil-mill waste waters (O), poultry litter (P) or meat bone meal (M) was used on the absorption of CO2 from a gas bottle. The absorption capacity (1.35 - 2.85 gCO2/gNH4) was bigger than other solvents such as ammonia and amines. The range of CO2 absorption was significantly bigger for the organic mixtures P and PM with urine (9.1 - 11.8) g/L than urine alone 6.5 g/L. These organic wastes could be used to increase CO2 absorption in urine and reduce gas emissions.
Algorithms of the Femtoscope: KeV X-Rays Cure Cancer While MeV X-Rays Only Burn the Cells  [PDF]
Edward Jiménez Calderon
American Journal of Computational Mathematics (AJCM) , 2018, DOI: 10.4236/ajcm.2018.84023
Abstract: The study of cancer with the Femtoscope shows us that the information of the cell nucleus is correlated with the atomic nucleus. Femtoscope and entropy algorithms monitor the time and energy of x-rays that transform cancer cells into healthy cells and vice versa. Curing cancer means recovering the information lost from a cancer cell, leading to a minimum entropy. The efficient treatment of cancer presents resonance frequencies in the production and elimination of cancerous cells, asymmetrically. The cure asymmetry of cancer is due to the support of DNA repair genes, allowing the stability of a race or species, and prioritizing life to death. Using the Femtoscope and Spectroscopy, we experimentally validate the resonance frequencies, which effectively cure the cancer and find the optimal times and doses of treatment. In this way, we minimize collateral effects and unnecessary economic costs. In addition, the phosphorus resonance demonstrates why the low energies of x-rays cure cancer and high x-ray energies only burn cancer cells.
Diffusion of Technology for Organizational Effectiveness: An Exploratory Study of the Procurement Department of a Multi-National Energy Company in Trinidad and Tobago  [PDF]
Prahalad Sooknanan, Jim Leung Chee
Advances in Journalism and Communication (AJC) , 2014, DOI: 10.4236/ajc.2014.24014
Abstract: This study investigates the diffusion of technology within a homogenous corporate cultural context according to age and educational level to determine how these variables determine the rate of diffusion. Based on the findings, the conventional communication models seem to apply. Specifically, the study shows that in the particular organizational context, age and education level impact on diffusion of technology. Younger and higher educated individuals seem to have a greater affinity to communication and use new communication technologies in their personal lives as well as professional lives which lend itself to possible early adoption or innovation. The study concludes, in the particular context, while hiring younger and more educated staff can possibly enhance the innovation and adoption process, it is perhaps equally, if not more important, to engage innovators from the wider organization to hasten the diffusion process.
Venter in new venture
Jim Kling
Genome Biology , 2002, DOI: 10.1186/gb-spotlight-20020816-01
Abstract: In April, Venter, a TIGR board member, announced the creation of the Center for the Advancement of Genomics, to study the public policy aspects of genomics, and the Institute for Biological Energy Alternatives, to investigate microbial sources of energy. Both projects, which are supported by Venter's Science Foundation, are adding a burden to TIGR's existing sequencing facility, and the new facility will ease that burden, Venter told us in a telephone interview.TIGR's existing facility, which now sequences genomes base by base, will eventually be converted to a genome closure facility that will fill in gaps at the end of projects. "For microbial genomes that (function) is very important, because 90% of most of the genome is genes, while only 1.1% of the human genome is genes. So gaps in humans don't really matter," Venter said.Venter also expects the new center to greatly decrease the cost of sequencing, while increasing speed, by using new high-throughput technology and microvolume procedures that should reduce the cost of reagents. The goal is to get full-genome sequencing down to a cost of $1,000 to $3,000 and to speed sequencing in order to "push the personalized medicine paradigm by sequencing genomes" in the time it takes to have a conversation," he said.The institute should open its doors by the end of the year. Although it does not yet have a title, there is an internal naming competition under way. "Outside submissions are certainly welcome, too," Venter said.
Why patient representation might harm science?
Jim Cassidy
Breast Cancer Research , 2007, DOI: 10.1186/bcr1802
Abstract: Clinical science in the form of clinical trials is a slow and laborious process. From concept to initiation, even a simple clinical trial can take several years and involves repeated applications for funding, peer review, ethics review and several other processes. The recent adoption of European Union directives on clinical trials has also added to this burden. Principal investigators are usually highly qualified and experienced individuals. They have taken the decision to lead research because they are committed to making a difference to patient care; otherwise, they could live out their working lives in the National Health Service (in the UK context) or in private practice (in most other countries). Although many would contend that they only do it because there is something in it for them – perhaps peer approval, fame, or power – I doubt that those rewards alone are sufficient motivation. This is borne out by the fact that clinical trialists are always in the minority of any professional health care grouping.Consultation of user groups/clients/customers is currently in vogue in many aspects of life. In most cases this is useful to obtain consumer advice about product or service qualities. Although one could draw parallels between these individuals and patients, I would contend that patients are not actually customers. This is because, in my view, becoming a customer is a voluntary process, whereas becoming a patient is as far from voluntary as one could imagine. There is also the implication in consumer feedback that things may change for the better as a result of that feedback; I am not convinced that this is realistic in many aspects of patient care. The customer can also shop elsewhere if they are unhappy with the product; once again, I am unconvinced that that fits with reality when one considers the provision of health care. Most of us are not within reasonable travelling distance of suitable alternatives, especially if one considers therapy for most life-thr
Is breast cancer advocacy distorting the cancer budget to the disadvantage of other tumours?
Jim Cassidy
Breast Cancer Research , 2007, DOI: 10.1186/bcr1804
Abstract: I think it is fair to say that cancer has (or will) touch all of us in a direct way. Hence, we all have close friends or family members who have the disease. Therapy for cancer is unfortunately often palliative because of late presentation and our lack of curative options for most metastatic common solid cancers, so there is much emotion surrounding cancer; some would suggest that this is out of proportion to the prevalence of the disease. Cardiovascular disease kills more people, but it is somehow less emotive to the general public. People who have a myocardial infarction do not ask 'How long have I got doc?', but cancer patients very often do ask for this kind of information.The most common cancers that we encounter are breast, lung, colorectal and urological. It is clear that the demographics of each of these diseases are distinct. Certain cancers have a predilection for age, sex and social class. Breast cancer is a disease predominantly of middle class females. It is actually commoner with increasing age, but middle-aged women still suffer a high proportion of the burden. Lung cancer almost exclusively occurs in smokers, and it has a higher incidence in lower social classes. Colorectal cancer is slightly more common in well educated people with sedentary occupations. Urological cancer (the bulk of which is prostate cancer) affects older males.Cancer therapy budgets have in the past been (and continue to be) established with little regard for the prevalence of the disease. In fact, the therapy budgets tend to reflect the number and type of options that we have available, and so breast cancer is out in front because we have multiple modalities of therapy (screening, surgery, radiotherapy, hormones and chemotherapy). This is in stark contrast to lung cancer, in which (until very recently) most patients presented late, had multiple smoking-induced comorbidities and could only be offered palliative care. Perhaps as we develop more and better therapies for other cance
Decoding the informational properties of the RNA polymerase II Carboxy Terminal Domain
Jim Karagiannis
BMC Research Notes , 2012, DOI: 10.1186/1756-0500-5-241
Abstract: By formally applying the long-standing mathematical principles of information theory, I explore the hypothesis that complex post-translational modifications of the CTD represent a means for the dynamic “programming” of Rpb1p and thus for the discrete modulation of the expression of specific gene subsets in eukaryotes.Empirical means for testing the informational capacity and regulatory potential of the CTD – based on simple genetic analysis in yeast model systems – are put forward and discussed.These ideas imply that the controlled manipulation of CTD effectors could be used to “program” the CTD and thus to manipulate biological processes in eukaryotes in a definable manner.The proper control of gene expression is crucial for a cell to grow, divide, and respond intelligently to environmental and/or developmental cues. Conversely, improper gene expression can lead to a variety of abnormal metabolic, developmental, and/or physiological states [1-7]. Of particular importance to this regulatory control in eukaryotes is the dynamic modulation of the phosphorylation status of the largest sub-unit of RNA polymerase II (Rpb1p) [8-10].RNA polymerase II has long been known to exist in both hyper- and hypo-phosphorylated forms (RNA pol IIo and RNA pol IIa, respectively). These two forms result from the regulated phosphorylation and de-phosphorylation of a carboxy-terminal extension found in the Rpb1p sub-unit [8-12]. This extension – referred to simply as the carboxy-terminal domain or CTD – is composed of tandem repeats of the heptapeptide sequence, Y1S2P3T4S5P6S7. The phosphorylation status of these repeats (the heptapeptides can be phosphorylated on Tyr-1, Ser-2, Thr-4, Ser-5, and/or Ser-7 residues) is controlled by 1) a sub-family of cyclin dependent kinases and 2) a group of small CTD phosphatases [8,10].While the number of repeats varies from species to species (15 copies in Microsporidia compared to a total of 52 in humans) the presence of this tandem set of heptapeptid
?Entrar no jogo?: pela Rússia, pelo dinheiro e pelo poder
Análise Social , 2006,
Abstract: developments in post-soviet football have to be seen against the cataclysmic socio-political changes that have occurred since the demise of communism and the ussr in 1991. football has acquired a new and unique meaning for ordinary people in terms of both nationhood and even ?apolitical? dreams. russian football today is in the hands of multibillionaire ?oligarchs? who use the sport mainly as a gloss over their less sporting activities and to launder their vast wealth. as a result, the russians are coming in world football, tsska?s victory in the uefa cup being just the first swallow of the russian summer. equally, the consequences of oligarch control could well cause huge upheaval throughout world football.
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