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Search Results: 1 - 10 of 44112 matches for " Jihua Wu "
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Effects of Winter Harvesting and Salinity on the Structure of Regrowing Reed Stands  [PDF]
Mingyao Huang, Qiang Sheng, Jihua Wu, Xiaoyun Pan
American Journal of Plant Sciences (AJPS) , 2014, DOI: 10.4236/ajps.2014.521339
Abstract: Harvest and salinity are two important factors of the growth of common reed (Phragmites australis). We here examined the different impact of winter harvest treatment on the Phragmites australis between high and low salinity (mean soil electrical conductivity is 7.87 ms/cm and 0.91 ms/cm) areas in Dongtan wetland of Yangtze River estuary, China. We measured phenology, Shoot density, stem diameter and height, biomass (total, aboveground and belowground biomass) and spike weight. At high salinity areas, harvest significantly inhibited the individual growth of stem height yet enhanced ramet density, and consequently led to an insignificant change in aboveground biomass. The belowground parts of reed were significantly inhibited. The root shoot ratio and the proportion of asexual reproduction decreased significantly. At low salinity areas, however, winter harvest generally has no significant influence on reed growth. Our results suggest that the winter harvest management may cause a decline of reed populations in the long term under condition of high salinity; however winter harvesting can be suggested as a proper management for sustainable utilization of reed communities at low salinity areas.
Research on Operating Performance of Small-Sized Retailers—Case Study of City S Market  [PDF]
Jihua Zhang
Open Journal of Business and Management (OJBM) , 2015, DOI: 10.4236/ojbm.2015.32022
Abstract: Previous studies have shown that small-sized retailers have weak competitiveness. This paper is intended to explore why they can survive in the market for a long time. It is proven that compared with large chain retailers, small-sized retailers are advantageous in employee utilization and gross margin despite of their weakness in commodity purchase and space utilization. Previous studies also reveal that small-sized retailers are significantly fewer in commercially developed regions than commercially underdeveloped regions, suggesting that it becomes harder for small-sized retailers to survive in the market along with gradual improvement of commercial facilities.
Advances in phylogenetic studies of Nematoda
Jihua Wu,Yanling Liang,Yang Zhong,Cuizhang Fu,Jiakuan Chen
Chinese Science Bulletin , 2002, DOI: 10.1360/02tb9002
Abstract: Nematoda is a metazoan group with extremely high diversity only next to Insecta. Caenorhabditis elegans is now a favorable experimental model animal in modern developmental biology, genetics and genomics studies. However, the phylogeny of Nematoda and the phylogenetic position of the phylum within animal kingdom have long been in debate. Recent molecular phylogenetic studies gave great challenges to the traditional nematode classification. The new phylogenies not only placed the Nematoda in the Ecdysozoan and divided the phylum into five clades, but also provided new insights into animal molecular identification and phylogenetic biodiversity studies. The present paper reviews major progress and remaining problems in the current molecular phylogenetic studies of Nematoda, and prospects the developmental tendencies of this field.
Descriptive Study of the Environmental Epidemiology of High Lung Cancer Incidence Rate in Qujing, Yunnan, China
Linlin ZHANG,Jihua LI,Yajie WANG,Guoping WU
Chinese Journal of Lung Cancer , 2012, DOI: 10.3779/j.issn.1009-3419.2012.03.05
Abstract: Background and objective Qujing, located in Southwest China, is an area with an extremely high lung cancer incidence. Combustion of coal has exposed local people to great health hazards. The aim of this study is to achieve a thorough understanding of the relationship between environmental pollution and the high incidence of lung cancer in Qujing, Yunnan Province, China. The results would provide a scientific basis and support for the etiology of lung cancer, as well as suggestions on improving the environmental conditions in the area. Methods A total of 280 rural villages were selected through stratified cluster random sampling. Environmental background and pollution were investigated, including details on fuel type, coking plant, metal smelting, and chemical plant, among others. Logistic regression analysis was used to analyze the investigated factors. Results Out of the total number of local villages studied, 78.1% of those with high incidence often use smoky coal and coking. On the other hand, 78.8% of the low-incidence areas use smokeless coal or wood. Logistic regression analysis indicated that the coal type used for everyday life was a main risk factor related to lung cancer (P<0.05). Using smoky and coking coals create an alarmingly high risk for developing lung cancer. Meanwhile, smokeless coals and wood seemed to have no significant relationship to the lung cancer incidence. Conclusion The fuel type used for everyday life is an important factor in the high incidence of lung cancer in Qujing. Evidently, the use of smoky coal and coke increased the incidence of lung cancer, whereas smokeless coal and wood seem to bring about the contrary.
Variability of Polychaete Secondary Production in Intertidal Creek Networks along a Stream-Order Gradient
Tianjiang Chu, Qiang Sheng, Sikai Wang, Jihua Wu
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0097287
Abstract: Dendritic tidal creek networks are important habitats for sustaining biodiversity and ecosystem functioning in salt marsh wetlands. To evaluate the importance of creek heterogeneity in supporting benthic secondary production, we assess the spatial distribution and secondary production of a representative polychaete species (Dentinephtys glabra) in creek networks along a stream-order gradient in a Yangtze River estuarine marsh. Density, biomass, and secondary production of polychaetes were found to be highest in intermediate order creeks. In high order (3rd and 4th) creeks, the density and biomass of D. glabra were higher in creek edge sites than in creek bottom sites, whereas the reverse was true for low order (1st and 2nd) creeks. Secondary production was highest in 2nd order creeks (559.7 mg AFDM m?2 year?1) and was ca. 2 folds higher than in 1st and 4th order creeks. Top fitting AIC models indicated that the secondary production of D. glabra was mainly associated with geomorphological characters including cross-sectional area and bank slope. This suggests that hydrodynamic forces are essential factors influencing secondary production of macrobenthos in salt marshes. This study emphasizes the importance of microhabitat variability when evaluating secondary production and ecosystem functions.
Detecting Overlapping Protein Complexes by Rough-Fuzzy Clustering in Protein-Protein Interaction Networks
Hao Wu, Lin Gao, Jihua Dong, Xiaofei Yang
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0091856
Abstract: In this paper, we present a novel rough-fuzzy clustering (RFC) method to detect overlapping protein complexes in protein-protein interaction (PPI) networks. RFC focuses on fuzzy relation model rather than graph model by integrating fuzzy sets and rough sets, employs the upper and lower approximations of rough sets to deal with overlapping complexes, and calculates the number of complexes automatically. Fuzzy relation between proteins is established and then transformed into fuzzy equivalence relation. Non-overlapping complexes correspond to equivalence classes satisfying certain equivalence relation. To obtain overlapping complexes, we calculate the similarity between one protein and each complex, and then determine whether the protein belongs to one or multiple complexes by computing the ratio of each similarity to maximum similarity. To validate RFC quantitatively, we test it in Gavin, Collins, Krogan and BioGRID datasets. Experiment results show that there is a good correspondence to reference complexes in MIPS and SGD databases. Then we compare RFC with several previous methods, including ClusterONE, CMC, MCL, GCE, OSLOM and CFinder. Results show the precision, sensitivity and separation are 32.4%, 42.9% and 81.9% higher than mean of the five methods in four weighted networks, and are 0.5%, 11.2% and 66.1% higher than mean of the six methods in five unweighted networks. Our method RFC works well for protein complexes detection and provides a new insight of network division, and it can also be applied to identify overlapping community structure in social networks and LFR benchmark networks.
The Impact of Complaints between Frontline Service Employees on Consumers—Based on Critical Incident Technique  [PDF]
Jihua Zhang, Ningning Wang
American Journal of Industrial and Business Management (AJIBM) , 2018, DOI: 10.4236/ajibm.2018.83054
Abstract: The critical incident technique was adopted to collect 174 critical events which were divided into 3 categories and 9 types about the complaint behaviors among the front-line service employees witnessed by the consumers. In addition, this paper discusses the influence mechanism of employees complaint behavior on consumers and enriches the theory research of employees' complaints and brand internalization.
Research on the Marketing Strategy of New Energy Vehicles in SL Company  [PDF]
Jihua Zhang, Rong Wang
American Journal of Industrial and Business Management (AJIBM) , 2019, DOI: 10.4236/ajibm.2019.92020
Abstract: New energy vehicle is a technological revolution in the automotive industry, which has changed the pattern of the original automotive industry to a certain extent. Nowadays, energy consumption and environmental pollution are becoming more and more serious, therefore new energy vehicles are becoming the trend of future industry development. Governments spare no efforts to promote new energy vehicles. In the case of its broad market prospects, the market gives new energy automotive enterprises a cold response. In addition to the heading companies, such as Tesla, small new energy automobile companies are still struggling to increase market share. Based on this, this paper mainly focuses on the marketing strategy of SL New Energy Automobile Company. Firstly, we overview the marketing theory, and introduce the SL new energy automobile company. Then we use PEST (Politics, Economy, Society, Technology) and SWOT (Strengths, Weaknesses, Opportunities, Threats) to analyze the macro and micro environment of SL company. Then we use STP (Segmentation, Targeting, Positioning) to decide the target market selection and positioning. Finally, we put forward the marketing strategy from the four aspects of SL company’s product, price, place and promotion. Through this paper, we hope to have better marketing suggestions for enterprise managers, and hope to help the new energy automobile enterprise to promote market share.
High resolution profiling of human exon methylation by liquid hybridization capture-based bisulfite sequencing
Junwen Wang, Hui Jiang, Guanyu Ji, Fei Gao, Mingzhi Wu, Jihua Sun, Huijuan Luo, Jinghua Wu, Renhua Wu, Xiuqing Zhang
BMC Genomics , 2011, DOI: 10.1186/1471-2164-12-597
Abstract: Here, we display a novel strategy that relies on the selective capture of target regions by liquid hybridization followed by bisulfite conversion and deep sequencing, which is referred to as liquid hybridization capture-based bisulfite sequencing (LHC-BS). To estimate this method, we utilized about 2 μg of native genomic DNA from YanHuang (YH) whole blood samples and a mature dendritic cell (mDC) line, respectively, to evaluate their methylation statuses of target regions of exome. The results indicated that the LHC-BS system was able to cover more than 97% of the exome regions and detect their methylation statuses with acceptable allele dropouts. Most of the regions that couldn't provide accurate methylation information were distributed in chromosomes 6 and Y because of multiple mapping to those regions. The accuracy of this strategy was evaluated by pair-wise comparisons using the results from whole genome bisulfite sequencing and validated by bisulfite specific PCR sequencing.In the present study, we employed a liquid hybridisation capture system to enrich for exon regions and then combined with bisulfite sequencing to examine the methylation statuses for the first time. This technique is highly sensitive and flexible and can be applied to identify differentially methylated regions (DMRs) at specific genomic locations of interest, such as regulatory elements or promoters.The methylation of cytosines at CpG dinucleotides is an important regulatory modification in the somatic cells of mammals and other vertebrates [1,2]. It plays a vital role in regulating gene transcription during diverse biological processes, including embryonic development, X-chromosome inactivation, and the maintenance of pluripotency and chromosome stability [3-8]. Aberrant DNA methylation is associated with many common diseases. Recent evidence has demonstrated that the epigenetic silencing of tumour suppressor genes due to abnormal DNA hypermethylation is involved in cancer development and p
Increased intracellular calcium concentration causes electrical turbulence in guinea pig ventricular myocytes
XinRong Fan,JiHua Ma,Wei Wan,PeiHua Zhang,Chao Wang,Lin Wu
Science China Life Sciences , 2011, DOI: 10.1007/s11427-011-4146-1
Abstract: Dysregulation of intracellular Ca2+ homeostasis is associated with various pathological conditions and arrhythmogenesis of the heart. The objective of this study was to investigate the effects of an acute increase in intracellular Ca2+ concentration ([Ca2+]i) on the electrophysiology of ventricular myocytes by mimicking intracellular Ca2+ overload. The [Ca2+]i was clamped to either a controlled (65–100 nmol L 1) or increased (1 μmol L 1) level. The transmembrane action potentials and ionic currents were recorded using whole-cell patch clamp techniques. We found that the acute increase in [Ca2+]i shortened the action potential duration, reduced the action potential amplitude, maximum depolarization velocity and resting membrane potential, caused delayed after-depolarizations (DADs), and triggered activity—compared with these parameters in the control. The increased [Ca2+]i augmented late I Na in a time-dependent manner, reduced I CaL and I K1, and increased I Kr but not I Ks. The results of this study can be used to explain calcium overload-induced ventricular arrhythmias.
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