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Search Results: 1 - 10 of 127109 matches for " Jianrong Li "
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Poly(I:C) promotes TNFα/TNFR1-dependent oligodendrocyte death in mixed glial cultures
Andrew J Steelman, Jianrong Li
Journal of Neuroinflammation , 2011, DOI: 10.1186/1742-2094-8-89
Abstract: To determine the effects of glial activation on oligodendrocyte viability we treated primary glial cultures isolated from neonatal rats or mice with the RNA viral mimic poly(I:C) and in some cases other TLR ligands. TLR3 expression was determined by western blot. Cytokine levels were measured by RT-PCR, ELISA, and intracellular cytokine staining. Oligodendrocyte precursor (preOL) viability was determined by Alamar blue assays and immunocytochemistry.Stimulation of mixed glial cultures with poly(I:C) resulted in microglia activation, TNFα production and preOL toxicity. This toxic effect of poly(I:C) was indirect as it failed to affect preOL viability in pure cultures despite the fact that preOLs express TLR3. Poly(I:C)-induced loss of preOLs was abolished in TNFα or TNFR1 deficient mixed glial cultures, suggesting that TNFα/TNFR1 signaling is required for poly(I:C) toxicity. Furthermore, although both microglia and astrocytes express functional TLR3, only microglia produced TNFα in culture. Consistent with these findings, other TLR agonists similarly triggered TNFα production and preOL toxicity in mixed glial cultures.Activation of microglia by poly(I:C) promotes TNFα/TNFR1-dependent oligodendroglial cell death. These data indicate that during an ongoing viral infection of the CNS, microglial TNFα may be detrimental to oligodendrocytes.The central nervous system (CNS) is considered immune privileged, but is not without a functional defense system [1]. Specifically, no organized lymphoid tissues or professional antigen presenting cells are thought to exist in the healthy CNS parenchyma. However, both resident microglia and astrocytes have been shown to possess immune surveillance properties and are thought to contribute to the chemoattraction and stimulation of antigen primed T-cells [2,3]. An innate ability to distinguish self from non-self is imperative for the limitation of pathogen spread, the stimulation of adaptive immune responses and the induction of tissue re
Duchesnea Phenolic Fraction Inhibits Tumor Growth through Restoring the Th1/Th2 Balance in U14 Cervical Cancer Bearing Mice  [PDF]
Bo Peng, Qin Hu, Lihua Sun, Xinmin Liu, Jianrong Li, Qi Chang, Liwei Wang, Jintian Tang
Chinese Medicine (CM) , 2012, DOI: 10.4236/cm.2012.31007
Abstract: Duchesnea indica (Andr.) Focke has been traditionally used to treat cancer in Asian countries for centuries. In the present study, transplanted U14 cervical cancer mouse model was used to evaluate the antitumor and immunomodulatory activity of Duchesnea phenolic fraction (DPF). ELISA and RIA assay were employed to measured the serum concentration of Th1/Th2 cytokines (IL-2, IL-4, IFN-γ and TNF-α). Administration with 0.25 g/kg, 0.5 g/kg and 1 g/kg DPF significantly reduced the tumor weight by 34.37%, 43.89% and 56.28%, respectively, as compared to the tumor control group. Furthermore, the serum level of IL-2, IFN-γ and TNF-α increased and IL-4 level decreased in a dose-dependent manner during DPF treatment, indicating that the antitumor activity of DPF may be associated with the decrease of TNF-α level and restoration of the balance of Th1/Th2 cell responses. These data suggested that DPF, a mixture of plant polyphenols, had potent anticancer activity which was in part accomplished by its immunomodulatory ability.
Research Progress on the Actinomyces arthrobacter  [PDF]
Huiling Fu, Yanfei Wei, Yanyan Zou, Mingzhi Li, Fangyuan Wang, Jianrong Chen, Lixin Zhang, Zhiheng Liu, Linxian Ding
Advances in Microbiology (AiM) , 2014, DOI: 10.4236/aim.2014.412081
Abstract: The genus Arthrobacter was established in 1947 by Conn & Dimmick. So far, more than 70 recognized species of the genus Arthrobacter have been certified. Its special functions have been widely known by researchers, such as, in agricultural, in medical, in industrial, and in environmental areas, etc. What deserves to be mentioned is that some species of genus Arthrobacter have showed the function of degrading pesticides, fixing nitrogen, producing beneficial enzyme, treating sewage, and so on. Recently, the applications of the genus Arthrobacter, especially the VBNC (viable but non-culturable) bacteria of this genus in the field of contaminated environment repair attract people’s attention and some related research results have also been obtained. The functions that we have known are waiting for us to study about mechanism, deeply. And, we can look forward to discovering more potential functions and applications of this genus in ecological environment. In the meantime, these discoveries must bring more new changes and knowledge.
Needs Analysis of Chinese Chemical Engineering and Technology Undergraduate Students in Yangtze University in English for Specific Purposes  [PDF]
Jianrong Wu, Yougen Lou
Creative Education (CE) , 2018, DOI: 10.4236/ce.2018.915195
Abstract: English for Specific Purposes (or ESP) is as a part of college English. This paper aims to investigate Chinese chemical engineering and technology undergraduate students’ needs in English for Specific Purposes (ESP). Eighty first-year chemical engineering and technology undergraduate students from Yangtze university as volunteer participates were in this study to answer the questionnaires about their needs in ESP and 60 volunteer participants were interviewed to provide their responses on ESP. All the data from questionnaires students answered and the interviews were collected. All the data were analyzed by SPSS 17. The results showed that:?1) The chemical engineering and technology undergraduate students’ level of proficiency in the language skills was medium; 2) The students ranked the language skills (listening and speaking) as the most important language skills; 3) Understanding instructions, following question/answer sessions, answering the questions, reading field-related textbooks, instructions for assignments, taking notes in lectures were more emphasized by the students in ESP classes; 4) The students had positive responses on the needs in ESP. Forty eight(80%) students thought that ESP would be better for their English learning and major study and thirty two (53.3%) students thought reading as the most important language skill ESP. Findings in the present study could be provided as implications for course designers and recommendations forfuture studies.
Characterization of 15 Polymorphic Microsatellite Loci for Cephalotaxus oliveri (Cephalotaxaceae), a Conifer of Medicinal Importance
Yingchun Miao,Xuedong Lang,Shuaifeng Li,Jianrong Su,Yuehua Wang
International Journal of Molecular Sciences , 2012, DOI: 10.3390/ijms130911165
Abstract: Cephalotaxus oliveri is a scarce medicinal conifer endemic to the south central region of China and Vietnam. A small fragmented population presently exists due to anthropogenic disturbance. C. oliveri has been used for its alkaloids harringtonine and homoharringtonine, which are effective against leucocythemia and lymphadenosarcoma. Monoecious plants have been detected in nature, although they were understood to be dioecious. In order to study the mating system, population genetics and the genetic effects of habitat fragmentation on C. oliveri, 15 polymorphic and 12 monomorphic microsatellite loci were developed for C. oliveri by using the Fast Isolation by AFLP of Sequences Containing repeats (FIASCO) protocol. The polymorphisms were assessed in 96 individuals from three natural populations (32 individuals per population). The number of alleles per locus ranged from two to 33, the observed and expected heterozygosity per locus ranged from 0.000 to 1.000 and from 0.000 to 0.923, respectively. These loci would facilitate a comprehensive understanding of the genetic dynamics on C. oliveri, which will be useful for establishing effective conservation strategies for this species.
Enzyme-Linked Immunosorbent-Assay for Deoxynivalenol (DON)
Fang Ji,Hua Li,Jianhong Xu,Jianrong Shi
Toxins , 2011, DOI: 10.3390/toxins3080968
Abstract: Deoxynivalenol (DON), one of the trichothecene mycotoxins, is a worldwide contaminant of wheat and barley, especially when infected by Fusarium graminearum, the causative agent of an epidemic wheat disease called Fusarium Head Blight. Because of the high risk of DON ingestion and the possibility of frequent exposure, it is important to develop a rapid and highly sensitive method for easy identification and quantification of DON in grain samples. In this study, we have developed an indirect competitive enzyme-linked immunosorbent assay (ELISA) to detect DON in wheat. We conjugated 3-O-Hemisuccinyl-DON (3HS-DON) to Bovine serum albumin (BSA) and Ovalbumin (OVA), and obtained DON-specific mice antisera. The indirect competitive ELISA revealed that the optimal concentration of mice serum and the coated antigen was 1/1600 and 1/1500, respectively. The antiserum cross-reacted with the trichothecenes 3-acetyl-DON and T-2 toxin, reaching about 55.2% and 6.3%, respectively, as compared with DON. Results showed that the assay could be performed satisfactorily using an extraction buffer containing less than 15% methanol. Recovery from DON was 82–93% in grains. The linear detection range of DON in grains was between 0.01 and 100 μg/mL.
Fabrication and magnetic properties of Co nanowire arrays of different crystal structures
Fashen Li,Tao Wang,Liyuan Ren,Jianrong Sun
Chinese Science Bulletin , 2004, DOI: 10.1360/03we0209
Abstract: Co nanowire arrays of different crystal structures have been electrodeposited into self-assembled anodic aluminum oxide (AAO) templates by varying pH value of electrolyte. The XRD results show that crystal structure of Co nanowires is fcc structure at pH = 2.5, a mixed structure of fcc and hcp at pH = 3.0 and 3.5, and hcp structure at pH = 5.0. Magnetic measurements indicate that Co nanowire arrays of different crystal structures exhibit different magnetic properties. The coercivity of Co nanowire array of fcc structure is larger than that of hcp structure about 900×103/4π A · m 1 along wire axis, and its squareness is also larger than that of hcp structure.
Structure and 57Fe conversion electron M ssbauer spectroscopy study of Mn-Zn ferrite nanocrystal thin films by electroless plating in aqueous solution
JianRong Sun,XueWen Wang,JinHong Liu,JianBo Wang,FaShen Li
Chinese Science Bulletin , 2008, DOI: 10.1007/s11434-008-0045-7
Abstract: Mn1 x Zn x Fe2O4 thin films with various Zn contents and of different thickness were synthesized on glass substrates directly by electroless plating in aqueous solution at 90°C without heat treatment. The Mn-Zn ferrite films have a single spinel phase structure and well-crystallized columnar grains growing perpendicularly to the substrates. The results of conversion electron 57Fe M ssbauer spectroscopy (CEMS) indicate that the cation distribution of Mn1 x Zn x Fe2O4 ferrite nanocrystal thin films fabricated by electroless plating is different from the bulk materials’ and a great quantity of Fe3+ ions are still present on A sites for x>0.5. When the Zn content of the films increases, Fe3+ ions in the films transfer from A sites to B sites and the hyperfine magnetic field reduces, suggesting that Zn2+ has strong chemical affinity towards the A sites. On the other side, with the increase of the thickness of the films, Fe3+ ions, at B sites in the spinel structure, increase and the array of magnetic moments no longer lies in the thin film plane completely. At x = 0.5, H c and M s of Mn1 x Zn x Fe2O4 thin films show a minimum of 3.7 kA/m and a maximum of 419.6 kA/m, respectively.
Mechanisms of Perinatal Brain Injury
Robin L. Haynes,Tara M. DeSilva,Jianrong Li
Neurology Research International , 2012, DOI: 10.1155/2012/157858
Evaluation of high-throughput functional categorization of human disease genes
Chen James L,Liu Yang,Sam Lee T,Li Jianrong
BMC Bioinformatics , 2007, DOI: 10.1186/1471-2105-8-s3-s7
Abstract: Background Biological data that are well-organized by an ontology, such as Gene Ontology, enables high-throughput availability of the semantic web. It can also be used to facilitate high throughput classification of biomedical information. However, to our knowledge, no evaluation has been published on automating classifications of human diseases genes using Gene Ontology. In this study, we evaluate automated classifications of well-defined human disease genes using their Gene Ontology annotations and compared them to a gold standard. This gold standard was independently conceived by Valle's research group, and contains 923 human disease genes organized in 14 categories of protein function. Results Two automated methods were applied to investigate the classification of human disease genes into independently pre-defined categories of protein function. One method used the structure of Gene Ontology by pre-selecting 74 Gene Ontology terms assigned to 11 protein function categories. The second method was based on the similarity of human disease genes clustered according to the information-theoretic distance of their Gene Ontology annotations. Compared to the categorization of human disease genes found in the gold standard, our automated methods can achieve an overall 56% and 47% precision with 62% and 71% recall respectively. However, approximately 15% of the studied human disease genes remain without GO annotations. Conclusion Automated methods can recapitulate a significant portion of classification of the human disease genes. The method using information-theoretic distance performs slightly better on the precision with some loss in recall. For some protein function categories, such as 'hormone' and 'transcription factor', the automated methods perform particularly well, achieving precision and recall levels above 75%. In summary, this study demonstrates that for semantic webs, methods to automatically classify or analyze a majority of human disease genes require significant progress in both the Gene Ontology annotations and particularly in the utilization of these annotations.
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