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Search Results: 1 - 10 of 5383 matches for " Jessica Ileana ángeles Solis "
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Pathology Isolation and Identification of Canine Herpesvirus (CHV-1) in Mexico  [PDF]
E. Guillermo Valdivia Lara, Blanca Lilia Barrón Romero, Laura Cobos Marín, Jessica Ileana ángeles Solis, Sofía González Gallardo, Cesar Cuenca Verde, Juan Antonio Montaraz Crespo, Guillermo Valdivia Anda
Open Journal of Pathology (OJPathology) , 2016, DOI: 10.4236/ojpathology.2016.63014
Abstract: This work presents the pathology description, isolation and identification of canine herpesvirus (CHV-1) in Mexico, a virus that causes a generalized hemorrhagic infection in puppies from the canidae family. Methods: Isolates were obtained from puppies that died within the first four weeks of life and had lesions consistent with canine herpesvirus. Results: The main gross lesions were petechial and ecchymotic hemorrhages in kidneys, liver and lungs; proliferative interstitial nephritis; multifocal necrosis in liver and kidneys; and encephalitis with intranuclear inclusion bodies. Herpesvirus was confirmed through direct immunofluorescence, electron microscopy and polymerase chain reaction for DNA polymerase and glycoprotein B genes. Discussion: Eight strains were isolated and identified as canine herpesvirus corresponding to three of the working cases with gross and microscopic lesions very similar to those described in the literature; then, isolates were confirmed by PCR gene amplification, positive reactions on immunofluorescence and observations from electron microscopy. This work represents the first report of this disease, including gross and histological lesions, and confirmation by isolation and identification of the canine herpesvirus in Mexico.
Canine Herpesvirus Seroprevalence and Associated Factors in Dogs of Mexico  [PDF]
Edgar Guillermo Valdivia Lara, Jesica Ileana ángeles Solis, Cesar Cuenca Verde, Juan Antonio Montaraz Crespo, Laura Cobos Marín, Juan Carlos Del Río García, Guillermo Valdivia Anda
Open Journal of Veterinary Medicine (OJVM) , 2016, DOI: 10.4236/ojvm.2016.610019
Abstract:
Canine herpesvirus (CHV-1) causes disease associated with high mortality in infect-ed puppies, which represents large financial losses for dog breeders. Since CHV-1 at the time of the study he had not been reported in Mexico, the main objective of this study was to determine the prevalence of antibodies against CHV-1 in canine kennels in the metropolitan area of Mexico City. A commercial enzyme-linked immuno-sorbent assay (ELISA) was used, and the results were compared to those of a viral neutralization test. The ELISA kit uses the complete viral particle as the antigen. The plaque reduction neutralization test was combined with the immunoperoxidase technique because of the low cytopathic effect of CHV-1. Neutralizing antibodies were also detected in 20 randomly selected samples. The prevalence of CHV-1 with ELISA was 87%. The concordance between ELISA and serum neutralization (SN) was 0.1129, the sensitivity of the ELISA against SN was 1.0 (100%), the positive predic-tive value was 0.39 (39%), and the negative predictive value was 1 (100%). These results show that ELISA is useful for monitoring the dog population for CHV-1; a positive test result requires confirmation with an SN test, and a negative ELISA result indicates a high probability of being SN-negative. The only variables that were sta-tistically associated with CHV-1 prevalence were breed and kennel. A statistically significant relationship between the degree of ELISA and SN titer was obtained, with a confidence level of 95%. None of the clinical presentation factors was statistically significant. These results suggest that most of the canine population studied in Mex-ico is in a herpesvirus latency state.
Stimulation of Phagocytosis and Production of Antibodies against Canine Herpesvirus Type 1 by Pidotimod (AdimodTM)  [PDF]
Edgar G. Valdivia Lara, Andrés ángeles Aldana, Jesica I. ángeles Solis, Patricia Miranda Castro, Juan C. Del Río García, Guillermo Valdivia Anda
Open Journal of Veterinary Medicine (OJVM) , 2017, DOI: 10.4236/ojvm.2017.74005
Abstract: Neutrophils are the most important circulating phagocytes. Circulating mono-cytes and precursors of tissue macrophages also have the ability to phagocytize. Pidotimod (ADIMODTM) exerts immunostimulatory and immunoregulatory effects through the stimulation and regulation of cellular immune responses by lymphocytes Canine herpesvirus (CHV) mainly affect puppies between the first and second weeks of age, causing high morbidity in the litter. To date, there is only one commercial vaccine in Europe to prevent disease. In this work, inactivated CHV cultures were inoculated in rabbits, adsorbed and not adsorbed to chitosan nanoparticles. Phagocytosis in the presence or absence of specific antibodies was measured. Response of virus neutralizing antibodies was also evaluated. AdimodTM enhanced the nonspecific and specific phagocytotic response. The association of the virus to the nanoparticles increased the phagocytic ability of blood cells; however, AdimodTM alone had a greater effect on phagocytic activity and generated a stronger immune response that corresponded to the increased phagocytosis (p < 0.05). Moreover, the level of neutralizing antibodies was higher and increased more rapidly when AdimodTM was used.
Circadian Genes Are Expressed during Early Development in Xenopus laevis
Kristen L. Curran, Silvia LaRue, Brittany Bronson, Jessica Solis, Aaron Trow, Nicole Sarver, Haisun Zhu
PLOS ONE , 2008, DOI: 10.1371/journal.pone.0002749
Abstract: Background Circadian oscillators are endogenous time-keeping mechanisms that drive twenty four hour rhythmic changes in gene expression, metabolism, hormone levels, and physical activity. We have examined the developmental expression of genes known to regulate circadian rhythms in order to better understand the ontogeny of the circadian clock in a vertebrate. Methodology/Principal Findings In this study, genes known to function together in part of the core circadian oscillator mechanism (xPeriod1, xPeriod2, and xBmal1) as well as a rhythmic, clock-controlled gene (xNocturnin) were analyzed using in situ hybridization in embryos from neurula to late tailbud stages. Each transcript was present in the developing nervous system in the brain, eye, olfactory pit, otic vesicle and at lower levels in the spinal cord. These genes were also expressed in the developing somites and heart, but at different developmental times in peripheral tissues (pronephros, cement gland, and posterior mesoderm). No difference was observed in transcript levels or localization when similarly staged embryos maintained in cyclic light were compared at two times of day (dawn and dusk) by in situ hybridization. Quantitation of xBmal1 expression in embryonic eyes was also performed using qRT-PCR. Eyes were isolated at dawn, midday, dusk, and midnight (cylic light). No difference in expression level between time-points was found in stage 31 eyes (p = 0.176) but stage 40 eyes showed significantly increased levels of xBmal1 expression at midnight (RQ = 1.98+/?0.094) when compared to dawn (RQ = 1+/?0.133; p = 0.0004). Conclusions/Significance We hypothesize that when circadian genes are not co-expressed in the same tissue during development that it may indicate pleiotropic functions of these genes that are separate from the timing of circadian rhythm. Our results show that all circadian genes analyzed thus far are present during early brain and eye development, but rhythmic gene expression in the eye is not observed until after stage 31 of development.
The Unexpected Bioenergetic Function of Neuromelanin. Implications in the Biology of Learning and Memory  [PDF]
Arturo Solis Herrera, Ruth I. Solis Arias
Natural Science (NS) , 2017, DOI: 10.4236/ns.2017.911036
Abstract: Progressive Recent Memory loss is the main complaint in Alzheimer’s disease patients. There is not a simple or congruous explanation yet due to highly complex mechanisms by which the Central Nervous System store and organize experiences every day, developed along 4 billion years of evolution; thereby learning and memory are poorly understood biological processes. The finding of the unsuspected intrinsic property of melanin to transforms light into chemical energy through water dissociation, as chlorophyll in plants; opens a new and wider horizon in the form in which the CNS generate and distribute chemical energy transporting it through molecular hydrogen produced by substantia nigra of mesencephalon and other pigmented structures. The mechanisms by which human modifies their behavior through learning and memory remain a mystery despite several decades of research. However, the?unexpected role of melanin molecule bioenergetics of first order, very above the chlorophyll and glucose, indicates?that the study of the fascinating processes involved in learning and memory several levels, needs to be started with the study of the generation and distribution of energy neuro-melanin-releasing to all the brain. In this article, we explain succinctly, the way hydrogen molecular (H2)-gas-, from the dissociation of water by the neuro melanin, moves from its source and permeates easily through the different structures of the Central Nervous System, as well as be the smaller atom, does not combine with water, moving through it in accordance with the laws of the simple diffusion. Molecular hydrogen is the main carrier of energy that nature uses in the entire universe, so that, once the neuro melanin separates it from the water, this displaces transporting its precious cargo of energy and its remarkable antioxidant capacity capable of reduce oxygen and form water.
Dynamical System and Nonlinear Regression for Estimate Host-Parasitoid Relationship
Ileana Miranda Cabrera,Heyker Lellanis Ba?os Díaz,María de los ángeles Martínez
Journal of Applied Mathematics , 2010, DOI: 10.1155/2010/851037
Abstract: The complex relationships of a crop with the pest, its natural enemies, and the climate factors exist in all the ecosystems, but the mathematic models has studied only some components to know the relation cause-effect. The most studied system has been concerned with the relationship pest-natural enemies such as prey-predator or host-parasitoid. The present paper shows a dynamical system for studying the relationship host-parasitoid (Diaphorina citri, Tamarixia radiata) and shows that a nonlinear model permits the estimation of the parasite nymphs using nymphs healthy as the known variable. The model showed the functional answer of the parasitoid, in which a point arrives that its density is not augmented although the number host increases, and it becomes necessary to intervene in the ecosystem. A simple algorithm is used to estimate the parasitoids level using the priori relationship between the host and the climate factors and then the nonlinear model. 1. Introduction The study of the biological control is of obligatory inclusion in the knowledge of the relationships between ecosystem factors to maintain the equilibrium of the components. In agriculture, a variation of this relation carries on an inequilibrium favorable to the pest. In these cases, an additional meddling is necessary for the control [1]. For this, models of the relationship occurring in an agriculture ecosystem have been developed in order to find the moment and densities permitting an inequilibrium. The Lotka-Volterra model shows the dynamical relation [2] and other models have been constructed with the same objective. Some authors have used this type of model to simulate processes, but without explaining the relationships existing in the ecosystem [3, 4]. Particularly, the Nicholson and Bailey model, or modifications of it, is used to describe the relation host-parasitoid [5]. They permit us to describe the influence of the host on the parasitoid dynamics [6]. But the models with exponential functions could be of chaotic behavior, even for a simple interaction [7]. Hence, statistical models can be used in some cases; however, the deterministic or stochastic models cannot be forgotten [8]. With the data of the samples, a statistical model can be found that proves the relationship host-parasitoid, and in the same way, the limit density for the control can be calculated. In particular, the relation between Diaphorina citri Kuwayana (Hemiptera:Psyllidae) and Tamarixia radaita Waterston (Hymenoptera:Eulophidae) was described using a deterministic system of 11 differential equations [7].
Estandarización de los métodos electroforéticos en geles de poliacrilamida en el Phast System y cromatográficos para la caracterización de nuevos antígenos vacunales y componentes de medios de cultivo
Ileana Martínez,María de los ángeles Padrón,Rubén Cabrera,Michel Acosta
Vaccimonitor , 2003,
Abstract: En este trabajo se realizó un análisis de diferentes parámetros que caracterizan la estandarización para la determinación del tama o molecular de nuevos antígenos de interés vacunal, a través deelectroforesis en “Phast System” y de distintas matrices cromatográficas. La evaluación electroforética de la linealidad en las curvas de patrones indicó valores de coeficientes decorrelación y determinación superiores a 0,98, con una adecuada repetibilidad; no se manifestaron diferencias entre los resultados obtenidos por varios analistas, en días diferentes yel método resultó ser exacto y robusto en las condiciones recomendadas. Se evaluaron los parámetros que caracterizan la eficiencia en el empaquetamiento de cuatro matricescromatográficas (Superosa 12, Sephacryl S-100, Sepharosa CL- 4B y Sephacryl S-1000). Los resultados obtenidos para ambos sistemas de determinación permitieron la evaluación satisfactoria de muestras de proteínas de membrana externa y lipopolisacárido, procedentes deVibrio cholerae, Leptospira ssp, Pseudomona aeruginosa y Shigella ssp.
Obtención y caracterización parcial de un extracto lipídico de la membrana externa de Mycobacterium smegmatis.
Nadine Alvarez,Reinier Borreo,María de los ángeles García,Ileana Martínez
Vaccimonitor , 2009,
Abstract: En la actualidad, los antígenos lipídicos de las micobacterias constituyen blancos atractivos para el desarrollo de nuevas formulaciones vacunales contra la tuberculosis. En nuestro trabajo se realizó la caracterización parcial de un extracto lipídico de pared celular de Mycobacterium smegmatis mediante cromatografía de capa delgada y Dot blot frente a gammaglobulina humana. Se identificó, fundamentalmente, la presencia de fosfolípidos y ácidos micólicos en el extracto lipídico y se observó un elevado reconocimiento de los mismos por la gammaglobulina humana, lo cual indica la importancia de continuar los estudios de inmunoprotección empleando antígenos lipídicos de micobacterias.
Urban Heat Island Mitigation and Urban Planning: The Case of the Mexicali, B. C. Mexico  [PDF]
Jorge Villanueva-Solis
American Journal of Climate Change (AJCC) , 2017, DOI: 10.4236/ajcc.2017.61002
Abstract: The derivations of climate change on cities and their activities depend on their capacity for adaptation and mitigation. In this sense, it has long recognized the influence of cities on their own climate, which is typically warmer than the surrounding. This phenomenon called urban heat island (UHI) has a number of impacts on air quality, water demand and energy. Since the fourth IPCC assessment report indicates the need for urban centers devoting efforts to adaptation to reduce the risks of direct and indirect impacts of climate change. The same organization recognizes the urban planning as a tool to seek such order. However, it also recognizes that the current scale of climate models cannot provide a representation of urban areas. This paper explores the intensification of the UHI, its relationship with urban expansion and its impact on housing in the city of Mexicali, B.C. Its aim is to determine its impact and mitigation potential through analysis and modeling of urban structure, expressed in use and land cover, as well as the implementation of mitigation strategies. The results show on the one hand, the convenience of using dynamic modeling as a tool applied to urban planning with a focus on mitigation and adaptation to climate change. Furthermore, regarding the implementation of strategies, the results show that most efficiency is obtained when applied generally, this is, considering green and cool roofs, cool pavements and afforestation as part of urbanization process, otherwise, only partial results are achieved. Overall, housing land use has significant potential to mitigate the UHI in the city.
PATRóN ESPACIAL Y PARáMETROS DE CRECIMIENTO DE Diaphorina citri KUWAYAMA Y SU PARASITOIDE Tamarixia radiata Waterston SOBRE Muraya paniculata
Miranda,Ileana; Ba?os Díaz,Heyker; Pérez Aranda,Yadira; de los ángeles Martínez,María;
Revista de Protecci?3n Vegetal , 2011,
Abstract: the spatial pattern and logistical growth of the asian citrus psyllid diaphorina citri kuwayama and its main parasitoid tamarixia radiata waterston on the crop muraya paniculata were studied to find out the correspondence of their spatial disposition, growth maximum capacity and intrinsic rate of increase. for it, weekly samplings were carried out in five points of the municipality playa, havana city. during two year (april 2007 - may 2009), five buds were randomly taken from three plants in each point and the d. citri densities evaluated. the parasite nymphs were observed until emerged adults of the parasitoid t. radiata when the density was also evaluated. taylor′s index and logistic model were used. both populations presented an aggregate pattern; the maximum capacity was of up to an average of 268,01 individuals per sampling for the host and 76,5 for its parasitoid. the intrinsic rate of increase were 0,153 for d. citri and 0,088 for t. radiate, the psyllid found favorable condition to reproduce.
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