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Search Results: 1 - 10 of 23427 matches for " Jeong-Ki Kim "
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Role of CD151, A tetraspanin, in porcine reproductive and respiratory syndrome virus infection
Kumar Shanmukhappa, Jeong-Ki Kim, Sanjay Kapil
Virology Journal , 2007, DOI: 10.1186/1743-422x-4-62
Abstract: We found that CD151, a host cellular protein, interacting with PRRSV 3' UTR RNA. The specificity of the interaction between CD151 and PRRSV 3' UTR RNA was examined by gel shift assay as well as North-Western hybridization. The transfection of CD151 expression clone into BHK-21 rendered these cells susceptible to PRRSV infection, and the transfection of siRNA against CD151 into MARC-145 significantly reduced the level of PRRSV infection. Also, anti-CD151 antibody treatment to MARC-145 completely blocked PRRSV infection.Based on our results, we suggest that CD151 should cooperate in PRRSV infection in vitro in MARC-145 and BHK-21 cells.Porcine reproductive and respiratory syndrome virus (PRRSV) is the causative agent of viral disease in swine that is endemic in swine producing regions throughout the world resulting in severe economic losses in affected areas. The disease is characterized by severe reproductive failure in sows and gilts and respiratory distress in pigs of all ages [1-3]. PRRSV is an enveloped virus containing single-stranded positive-sense RNA as the genome. Its genome is 14.5 kb in length and is composed of nine open reading frames (ORFs; ORF 1a, ORF 1b, ORF 2a, ORF 2b, ORF 3, ORF 4, ORF 5, ORF 6 and ORF 7) flanked by 5' and 3' untranslated regions (UTRs) [4,5]. PRRSV belongs to the family Arteriviridae, grouped together with the Coronaviridae and Roniviridae in the order Nidovirales [6-8]. Other members in the family Arteriviridae include equine arteritis virus, lactate dehydrogenase-elevating virus of mice, and simian hemorrhagic fever virus [9].PRRSV has a restricted cell tropism in its host (pig). It primarily infects alveolar macrophages although the virus has been detected in macrophages of other tissues like spleen, liver, peyers patches, thymus as well as microglial cells, however peritoneal macrophages are refractory [10,11]. Primary cultures of porcine alveolar macrophages are routinely used for in vitro isolation of PRRSV. Other established
Measurement of Electrical Characteristics of Female Breast Tissues for the Development of the Breast Cancer Detector
Tae-Hong Kim;Jeong-Ki Pack
PIER C , 2012, DOI: 10.2528/PIERC12050704
Abstract: In this paper, dielectric characteristics of female breast tissues were measured. Breast Tissues were mainly composed of fat, fibro-glandular and tumor. Measured tissues were directly extracted from mice and a rat just before the measurements to maintain the tissues as fresh as living ones before degeneration. This makes the measured results more accurate. Because the extracted tissues were very thin, they were measured by two methods using HP probe and a newly designed two-port sample holder. Numerical results for the two-port sample holder were obtained for both the forward and inverse problems. Dielectric properties of breast tissues were measured in the frequency range between 50 MHz and 5 GHz. We calculated the electrical constant with the measured data from the two-port sample holder. As a result of the measurement, the dispersion characteristics of the female breast tissues were fitted into the first Cole-Cole model.
Gene expression profile of the skin in the 'hairpoor' (HrHp) mice by microarray analysis
Bong-Kyu Kim, In-Cheol Baek, Hwa-Young Lee, Jeong-Ki Kim, Hae-Hiang Song, Sungjoo K Yoon
BMC Genomics , 2010, DOI: 10.1186/1471-2164-11-640
Abstract: From 45,282 mouse probes, differential expressions in 43 (>2-fold), 306 (>1.5-fold), and 1861 genes (>1.2-fold) in skin from HrHp/HrHp mice were discovered and compared with skin from wild-type mice. Among the 1861 genes with a > 1.2-fold increase in expression, further analysis showed that the expression of eight genes known to have a close relationship with hair follicle development, ascertained by conducting real-time PCR on skin RNA produced during hair follicle morphogenesis (P0-P14), indicated that four genes, Wif1, Casp14, Krt71, and Sfrp1, showed a consistent expression pattern with respect to HR overexpression in vivo.Wif1 and Casp14 were found to be upregulated, whereas Krt71 and Sfrp1 were downregulated in cells overexpressing HR in transient transfection experiments on keratinocytes, suggesting that HR may transcriptionally regulate these genes. Further studies are required to understand the mechanism of this regulation by the HR cofactor.With a complex and dynamic structure, hair is generated by hair-producing follicles and has a patterned cycle of growth and remodeling, which consists of growth (anagen), regression (catagen), and rest (telogen) stages. There are many genes involved in mature hair follicle (HF) regulation [1].One of these genes, hairless (Hr), is expressed in skin, specifically in the suprabasal cell layer of the interfollicular epidermis and in the lower portion of the HF epithelium; its expression is dependent on the hair cycle. Hr encodes a 130 kDa protein (HR), which contains a zinc finger domain and is localized in the nucleus [2], and acts as a transcriptional corepressor that regulates transcription through directly binding to the thyroid hormone receptor [3,4], vitamin D receptor [5], and retinoic acid-like orphan receptor α [6].Various Hr mutant mice have been studied to understand the function of HR, and most Hr mutant mice are created by causing the loss of HR function in their cells, giving them a typical phenotype with a re
Protective Efficacy of a Human Endogenous Retrovirus Envelope-Coated, Nonreplicable, Baculovirus-Based Hemagglutin Vaccine against Pandemic Influenza H1N1 2009
Jae-Yoo Choi, Yong-Dae Gwon, Jeong-Ki Kim, Yeon-Dong Cho, Yoon-Ki Heo, Han-Sam Cho, Tae-Jin Choi, Ha-Ryoung Poo, Yu-Kyoung Oh, Young Bong Kim
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0080762
Abstract: Despite the advantages of DNA vaccines, overcoming their lower efficacy relative to that of conventional vaccines remains a challenge. Here, we constructed a human endogenous retrovirus (HERV) envelope-coated, nonreplicable, baculovirus-based HA vaccine against swine influenza A/California/04/2009(H1N1) hemagglutin (HA) (AcHERV-sH1N1-HA) as an alternative to conventional vaccines and evaluated its efficacy in two strains of mice, BALB/c and C57BL/6. A commercially available, killed virus vaccine was used as a positive control. Mice were intramuscularly administered AcHERV-sH1N1-HA or the commercial vaccine and subsequently given two booster injections. Compared with the commercial vaccine, AcHERV-sH1N1-HA induced significantly higher levels of cellular immune responses in both BALB/c and C57BL/6 mice. Unlike cellular immune responses, humoral immune responses depended on the strain of mice. Following immunization with AcHERV-sH1N1-HA, C57BL/6 mice showed HA-specific IgG titers 10- to 100-fold lower than those of BALB/c mice. In line with the different levels of humoral immune responses, the survival of immunized mice after intranasal challenge with sH1N1 virus (A/California/04/2009) depended on the strain. After challenge with 10-times the median lethal dose (MLD50) of sH1N1 virus, 100% of BALB/c mice immunized with the commercial vaccine or AcHERV-sH1N1-HA survived. In contrast, C57BL/6 mice immunized with AcHERV-sH1N1-HA or the commercial vaccine showed 60% and 70% survival respectively, after challenge with sH1N1 virus. In all mice, virus titers and results of histological analyses of lung tissues were consistent with the survival data. Our results indicate the importance of humoral immune response as a major defense system against influenza viral infection. Moreover, the complete survival of BALB/c mice immunized with AcHERV-sH1N1-HA after challenge with sH1N1 virus suggests the potential of baculoviral vector-based vaccines to achieve an efficacy comparable to that of killed virus vaccines.
Dual-Specificity Phosphatase 10 Controls Brown Adipocyte Differentiation by Modulating the Phosphorylation of P38 Mitogen-Activated Protein Kinase
Hye-Ryung Choi, Won Kon Kim, Eun Young Kim, Baek Soo Han, Jeong-Ki Min, Seung-Wook Chi, Sung Goo Park, Kwang-Hee Bae, Sang Chul Lee
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0072340
Abstract: Background Brown adipocytes play an important role in regulating the balance of energy, and as such, there is a strong correlation between obesity and the amount of brown adipose tissue. Although the molecular mechanism underlying white adipocyte differentiation has been well characterized, brown adipocyte differentiation has not been studied extensively. Here, we investigate the potential role of dual-specificity phosphatase 10 (DUSP10) in brown adipocyte differentiation using primary brown preadipocytes. Methods and Results The expression of DUSP10 increased continuously after the brown adipocyte differentiation of mouse primary brown preadipocytes, whereas the phosphorylation of p38 was significantly upregulated at an early stage of differentiation followed by steep downregulation. The overexpression of DUSP10 induced a decrease in the level of p38 phosphorylation, resulting in lower lipid accumulation than that in cells overexpressing the inactive mutant DUSP10. The expression levels of several brown adipocyte markers such as PGC-1α, UCP1, and PRDM16 were also significantly reduced upon the ectopic expression of DUSP10. Furthermore, decreased mitochondrial DNA content was detected in cells expressing DUSP10. The results obtained upon treatment with the p38 inhibitor, SB203580, clearly indicated that the phosphorylation of p38 at an early stage is important in brown adipocyte differentiation. The effect of the p38 inhibitor was partially recovered by DUSP10 knockdown using RNAi. Conclusions These results suggest that p38 phosphorylation is controlled by DUSP10 expression. Furthermore, p38 phosphorylation at an early stage is critical in brown adipocyte differentiation. Thus, the regulation of DUSP10 activity affects the efficiency of brown adipogenesis. Consequently, DUSP10 can be used as a novel target protein for the regulation of obesity.
TXNIP Deficiency Exacerbates Endotoxic Shock via the Induction of Excessive Nitric Oxide Synthesis
Young-Jun Park equal contributor,Sung-Jin Yoon equal contributor,Hyun-Woo Suh,Dong Oh Kim,Jeong-Ran Park,Haiyoung Jung,Tae-Don Kim,Suk Ran Yoon,Jeong-Ki Min,Hee-Jun Na,Seon-Jin Lee,Hee Gu Lee,Young Ho Lee,Hee-Bong Lee,Inpyo Choi
PLOS Pathogens , 2013, DOI: 10.1371/journal.ppat.1003646
Abstract: Thioredoxin-interacting protein (TXNIP) has multiple functions, including tumor suppression and involvement in cell proliferation and apoptosis. However, its role in the inflammatory process remains unclear. In this report, we demonstrate that Txnip?/? mice are significantly more susceptible to lipopolysaccharide (LPS)-induced endotoxic shock. In response to LPS, Txnip?/? macrophages produced significantly higher levels of nitric oxide (NO) and inducible nitric oxide synthase (iNOS), and an iNOS inhibitor rescued Txnip?/? mice from endotoxic shock-induced death, demonstrating that NO is a major factor in TXNIP-mediated endotoxic shock. This susceptibility phenotype of Txnip?/? mice occurred despite reduced IL-1β secretion due to increased S-nitrosylation of NLRP3 compared to wild-type controls. Taken together, these data demonstrate that TXNIP is a novel molecule that links NO synthesis and NLRP3 inflammasome activation during endotoxic shock.
Induction of Hair Growth by Insulin-Like Growth Factor-1 in 1,763 MHz Radiofrequency-Irradiated Hair Follicle Cells
Sun-Young Yoon, Kyu-Tae Kim, Seong Jin Jo, A-Ri Cho, Soon-Ik Jeon, Hyung-Do Choi, Kyu Han Kim, Gun-Sik Park, Jeong-Ki Pack, Oh Sang Kwon, Woong-Yang Park
PLOS ONE , 2011, DOI: 10.1371/journal.pone.0028474
Abstract: Radiofrequency (RF) radiation does not transfer high energy to break the covalent bonds of macromolecules, but these low energy stimuli might be sufficient to induce molecular responses in a specific manner. We monitored the effect of 1,763 MHz RF radiation on cultured human dermal papilla cells (hDPCs) by evaluating changes in the expression of cytokines related to hair growth. The expression of insulin-like growth factor-1 (IGF-1) mRNA in hDPCs was significantly induced upon RF radiation at the specific absorption rate of 10 W/kg, which resulted in increased expression of B-cell chronic lymphocytic leukemia/lymphoma 2 (BCL-2) and cyclin D1 (CCND1) proteins and increased phosphorylation of MAPK1 protein. Exposure to 10 W/kg RF radiation 1 h per day for 7 days significantly enhanced hair shaft elongation in ex vivo hair organ cultures. In RF-exposed follicular matrix keratinocytes in the hair bulb, the expression of Ki-67 was increased, while the signal for terminal deoxynucleotidyl transferase dUTP nick end labeling was reduced. From these results, we suggest that 1,763 MHz RF exposure stimulates hair growth in vitro through the induction of IGF-1 in hDPCs.
Combined Sciatic, Femoral, and Obturator Nerve Blocks for Ankle Surgery in a Patient with Severe Pulmonary Hypertension—A Case Report  [PDF]
Ju Hyun Lee, Hyun Su Shin, Ki Yoon Kim, Ji Seon Jeong, Justin Sangwook Ko
Open Journal of Anesthesiology (OJAnes) , 2017, DOI: 10.4236/ojanes.2017.78022
Abstract: We report a patient with severe pulmonary arterial hypertension (PAH) undergoing tibio-talo-calcaneal fusion due to Charcot joint. Despite the advancement in the management of PAH, the risks of anesthesia, surgery, and postoperative morbidity and mortality still remain high. A 46-year-old female was presented with severe PAH and end stage renal disease requiring hemodialysis three times a week. Ultrasound-guided sciatic, femoral, and obturator nerve blocks were performed with 0.5% levobupivacaine 15 ml, 10 ml, and 5 ml, respectively. All the blocks were successful, and the patient underwent uneventful anesthesia and surgery. In addition, the postoperative pain control lasted for 15 h and the patient was discharged on POD 5 without any complications. Therefore, ultrasound-guided sciatic, femoral, and obturator nerve blocks are valuable alternative to the general or neuraxial anesthesia in patients with severe pulmonary hypertension.
Effect of Sodium Bicarbonate Administration on Mortality in Patients with Lactic Acidosis: A Retrospective Analysis
Hyun Jeong Kim, Young Ki Son, Won Suk An
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0065283
Abstract: Background Lactic acidosis is a common cause of high anion gap metabolic acidosis. Sodium bicarbonate may be considered for an arterial pH <7.15 but paradoxically depresses cardiac performance and exacerbates acidosis by enhancing lactate production. This study aimed to evaluate the cause and mortality rate of lactic acidosis and to investigate the effect of factors, including sodium bicarbonate use, on death. Methods We conducted a single center analysis from May 2011 through April 2012. We retrospectively analyzed 103 patients with lactic acidosis among 207 patients with metabolic acidosis. We used SOFA and APACHE II as severity scores to estimate illness severity. Multivariate logistic regression analysis and Cox regression analysis models were used to identify factors that affect mortality. Results Of the 103 patients with a mean age of 66.1±11.4 years, eighty-three patients (80.6%) died from sepsis (61.4%), hepatic failure, cardiogenic shock and other causes. The percentage of sodium bicarbonate administration (p = 0.006), catecholamine use, ventilator care and male gender were higher in the non-survival group than the survival group. The non-survival group had significantly higher initial and follow-up lactic acid levels, lower initial albumin, higher SOFA scores and APACHE II scores than the survival group. The mortality rate was significantly higher in patients who received sodium bicarbonate. Sodium bicarbonate administration (p = 0.016) was associated with higher mortality. Independent factors that affected mortality were SOFA score (Exp (B) = 1.72, 95% CI = 1.12–2.63, p = 0.013) and sodium bicarbonate administration (Exp (B) = 6.27, 95% CI = 1.10–35.78, p = 0.039). Conclusions Lactic acidosis, which has a high mortality rate, should be evaluated in patients with metabolic acidosis. In addition, sodium bicarbonate should be prescribed with caution in the case of lactic acidosis because sodium bicarbonate administration may affect mortality.
Metal insulator transitions in perovskite SrIrO3 thin films
Abhijit Biswas,Ki-Seok Kim,Yoon Hee Jeong
Physics , 2013, DOI: 10.1063/1.4903314
Abstract: Understanding of metal insulator transitions in a strongly correlated system, driven by Anderson localization (disorder) and/or Mott localization (correlation), is a long standing problem in condensed matter physics. The prevailing fundamental question would be how these two mechanisms contrive to accomplish emergent anomalous behaviors. Here, we have grown high quality perovskite SrIrO3 thin films, containing a strong spin orbit coupled 5d element Ir, on various substrates such as GdScO3 (110), DyScO3 (110), SrTiO3 (001), and NdGaO3 (110) with increasing lattice mismatch, in order to carry out a systematic study on the transport properties. We found that metal insulator transitions can be induced in this system; by either reducing thickness (on best lattice matched substrate) or changing degree of lattice strain (by lattice mismatch between film and substrates) of films. Surprisingly these two pathways seek two distinct types of metal insulator transitions; the former falls into disorder driven Anderson type whereas the latter turns out to be of unconventional Mott-Anderson type with the interplay of disorder and correlation. More interestingly, in the metallic phases of SrIrO3, unusual non-Fermi liquid characteristics emerge in resistivity as the resistivity exponent evolves from from 4/5 to 1 to 3/2 with increasing lattice strain. We discuss theoretical implications of these phenomena to shed light on the metal insulator transitions.
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