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Search Results: 1 - 10 of 2063 matches for " Jelena Popovi? "
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Leishmaniasis in dogs: Case study
Aleksi? Jelena,Kukolj Vladimir,Popovi? Nikola
Veterinarski Glasnik , 2009, DOI: 10.2298/vetgl0904251a
Abstract: The paper presents a case of leishmaniasis in a 2.5-month-old dog imported from France. The clinical examination established a generally poor state of health, expressed cachexia, atrophy of the temporal musculature, weakness of movement, as well as abnormally long and brittle nails. There was also hyperkeratosis of the nose tip and paws. A histological examination of biopsy sections of the altered skin parts showed inflammatory changes in the area of the dermis, together with infiltration of macrophages and a smaller number of lymphocytes, plasmocytes and neutrophil granulocytes in the area around the sebaceous glands and hair follicles. The determined changes correspond to superficial dermatitis. Edema followed by partial degeneration of connective-tissue fibers is observed in connective tissue. A smaller number of intracellular parasitic forms was established in mononuclear cells. A smaller number of oval amastigotes with round dark red nucleis were observed in sections stained using the Gimza method in the cytoplasm of macrophages located in the dermis, but also extracellularly. It was concluded that the dog was diseased with leishmaniasis on the grounds of the clinical picture and the microscopic findings.
Active biomonitoring of air radioactivity in urban areas
Popovi? Dragana,Todorovi? Dragana,Ajti? Jelena,Nikoli? Jelena
Nuclear Technology and Radiation Protection , 2009, DOI: 10.2298/ntrp0902100p
Abstract: To assess the validity of the moss bag monitoring technique in the radioactivity control of ground level urban air, a study on radionuclide contents in moss was performed in the city of Belgrade, Serbia. From May 2006 to May 2007, moss (Sphagnum girgensohni, Dubna, Russia) was exposed to the aero pollution in a location in the central area of the city. The activity of 40K, 210Pb, and 137Cs was measured on an HPGe detector (Canberra, relative efficiency 23%) by standard gamma spectrometry. The activities 245 ± 25 Bq/kg for 40K, 315 ± 34 Bq/kg for 210Pb, and 28 ± 4 Bq/kg for 137Cs are in the range of values reported for the region; the differences are due to the moss species, local climate and measuring technique. Taking into consideration the time of the exposure and appropriate calibration procedure, moss bag biomonitoring could be used as a complementary method for determination of radionuclides in urban air.
Jelena Luji?,Desanka Kosti?,Ester Popovi,Miroslav ?irkovi?
Ribarstvo : Croatian Journal of Fisheries , 2012,
Abstract: On the basis of the data published between 1983 and 2008, this paper shows presence of tench (Tinca tinca), carp (Cyprinus carpio), pike (Esox lucius), catfish (Silurus glanis), zander (Sander lucioperca) and sterlet (Acipenser ruthenus) in the Gornje Podunavlje and Koviljsko-Petrovaradinski Rit Special Nature Reserves and the Bege ka Jama Nature Park, as well as in the part of the Danube’s bed between river kilometre 1249- and 1255. The number of these fish species in the River Danube and its flood zones in this part of the Pannonian Basin is decreasing due to the use of inappropriate “tools”, electric current, uncontrolled agricultural and industrial waste dumps, degradation of the habitat. The above mentioned species deserve attention because, according to the Rulebook on declaration and protection of protected and strictly protected wild species of plants, animals and fungi of 2010, in Serbia, T. tinca is a strictly protected species, while C. carpio, E. lucius, S. glanis, S. lucioperca and A. ruthenus are listed as protected. On the basis of the 2009 Fisheries Law in Serbia, permanently closed fishing season was introduced for tench, and closed season in certain periods and fishing ban for individuals under the prescribed size for the rest of the above mentioned species. A. ruthenus is subject to the Law on Ratification of Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES), ANNEX II and is listed in the category of species that may be in danger of extinction. Furthermore, it can be found on the IUCN Red List of Endangered Species of the International Union for Conservation of Nature – VU, which means that it is marked as vulnerable.
Analysis of the anti-apoptotic protein bcl-2 in oral squamous cell carcinoma
Popovi? Branka,Jeki? Biljana,Novakovi? Ivana,Mila?in Jelena
Stomatolo?ki Glasnik Srbije , 2007, DOI: 10.2298/sgs0703153p
Abstract: Aim: The aim of this study was to analyze the presence of the anti-apoptotic protein bcl-2 in oral squamous cell carcinoma and determine its potential role in the development and progression of this type of tumor. Materials and methods: The expression of bcl-2 was determined in 28 paraffin blocks of oral squamous cell carcinoma using the immunohistochemical method. The percentage of the immuno-reactive cells in positively stained tumor regions was determined using the microscopic analysis and Ozaria software. Results: Positive immunohistochemical test was observed in 19 out of 28 samples (68%) as follows: in 11 samples there was a low (+), in four a moderate (++) and in the last four a high percentage (+++) of stained cells. In the group of patients at the low stage of the disease (T2), 50% of tumor samples showed bcl-2 protein expression whereas in the higher stages (T3 and T4) of positively stained samples, this percentage was 67%. There was a trend of an increasing number of cells with positive bcl-2 staining in the tumors of higher clinical stages but not the level of bcl-2 protein expression. Conclusion: Both parameters, the presence of bcl-2 staining and the percentage of cells with bcl-2 immunoexpression, may act as additional prognostic parameters that indicate an increased proliferative tumor potential.
Effectiveness of different methods of decontamination of endodontic instruments
PopoviJelena,Ga?i? Jovanka,Stojilkovi? Goran,Da?i? Stefan
Stomatolo?ki Glasnik Srbije , 2008, DOI: 10.2298/sgs0802088p
Abstract: Introduction: There is an increasing awareness of the risk of iatrogenic transmission of prion diseases, since this infective agent has shown resistance to conventional procedures of chemical and thermal decontamination and sterilisation. Aim: The aim of the study was to evaluate the effectiveness of different methods for biological debris removal from the surfaces of endodontic instruments and to suggest an efficient protocol for cleansing prior to sterilization. Materials and Methods: Eighty four new endodontic instruments were used for hand instrumentation of the root canals in extracted teeth in order to accumulate organic debris. The instruments were allocated to four groups and subjected to different methods of decontamination: mechanical cleansing with a brush and sponge, chemical dissolution in sodium hypochlorite (NaOCl) and enzyme cleanser, ultrasonic treatment in water and disinfectant and the combination of the most effective mechanical, chemical and ultrasonic methods. The instruments were stained in the Van Gieson solution which efficiently stains the organic component and then analyzed under a light microscope. Results: Effectiveness was evaluated based on the amount of residual organic material. The analysis of pairs of methods with similar effects showed significant differences between mechanical cleansing with a brush and sponge and ultrasonic treatment in water and disinfectant. No difference was found between treatments in NaOCl and enzyme cleanser. The combined method was significantly more efficient than the other methods. Conclusion: The most efficient protocol for endodontic instrument decontamination consists of mechanical cleansing with a sponge soaked in chlor hexidine, chemical treatment in the enzyme cleanser and ultrasonic treatment in an appropriate disinfectant.
Analysis of microsatellite markers D18S70 and d20S116 in DNA isolated from dentin: Use in forensic medicine
Puzovi? Dragana,Popovi? Branka,Novakovi? Ivana,Mila?in Jelena
Srpski Arhiv za Celokupno Lekarstvo , 2009, DOI: 10.2298/sarh0902043p
Abstract: Introduction. Short tandem repeats and more specifically microsatellites represent a powerful tool in forensic medicine. In the past years, they have been extensively used in human identification and paternity testing. Objective The aim of the present study was to analyze two microsatellite markers in the Serbian population, i.e. to determine the number of alleles and the relevant forensic parameters. Methods. DNA was isolated from teeth samples using standard proteinase K digestion and phenol/chloroform alcohol extraction. PCR products were analyzed on polyacrilamide gels and visualized by AgNO3 staining. Forensic parameters were calculated using the Cervus software. Results. The loci D18S70 and D20S116 were analyzed on a sample of 70 unrelated, healthy adult individuals from Serbia. The number of alleles was determined and Hardy Weinberg equilibrium was confirmed for both loci. D18S70 and D20S116 demonstrated 6 and 8 alleles, respectively. The power of discrimination (PD) and the power of exclusion (PE) for the tested STR loci, D18S70 and D20S116 were 0.92 (PD), 0.41 (PE) and 0.95 (PD), 0.480 (PE), respectively. Conclusion. According to the presented data, D18S70 and D20S116 are most informative markers. Based on allelic frequencies and statistical parameters for forensic testing, it may be suggested that these two microsatellites represent useful markers for individual identification and parentage analysis in the Serbian population.
The investigation of ultrasound efficacy in cleaning the surface of new endodontic instruments
PopoviJelena,Ga?i? Jovanka,Radi?evi? Goran
Srpski Arhiv za Celokupno Lekarstvo , 2009, DOI: 10.2298/sarh0908357p
Abstract: Introduction. Active parts and tips of various new stainless steel and nickel-titanium endodontic instruments can be coated with fragments or metal residues, which can become detached during endodontic treatment. These fragments may obstruct the root canals or even reach the periapical tissue during biomechanical preparation and should be removed before clinical use. Objective. The aim of this study was to evaluate the presence of metal residues on both new stainless steel and nickel-titanium endodontic instruments, and to determine the cleaning efficacy of ultrasound using distilled water or disinfectant solution for removing these residues. Methods. Forty-eight stainless steel and nickel-titanium instruments were carefully removed from their original packages with dental tweezers, in order to avoid any contact with the cutting flutes and tips. The instruments were evaluated in term of metal debris presence, using scanning electron microscopy (SEM) and x-ray energy-dispersive spectroscopy (EDS). The instruments were then removed from the electron microscopy analysis stubs and placed in an ultrasonic bath for 15 minutes at a frequency of 28 kHz, using distilled water or disinfectant solution. The surfaces of the instruments were re-evaluated after cleaning. Results. Before ultrasound cleaning, a larger amount of metal debris was observed on the nickel-titanium endodontic instruments when compared to those made of stainless steel. The presence of metal particles on the instruments was evaluated by using EDS analysis. The use of ultrasound was effective in removing the metal residues from both types of endodontic instrument surfaces. Conclusion. The use of ultrasound proved to be an efficient method for the removal of metal particles from the surface of new stainless steel and nickel-titanium endodontic instruments.
Use of vacuum-assisted closure device in a disastrous form of abdominal sepsis and stoma site infection: Systematic review and report of a case
Popovi? Milo?,Bari?i? Goran,Markovi? Velimir,Petrovi? Jelena
Acta Chirurgica Iugoslavica , 2012, DOI: 10.2298/aci1202111p
Abstract: Use of Vacuum-Assisted Closure (VAC) for treatment of open abdomen has been established predominantly in cases of severe abdominal trauma, resulting with high percentage of primary fascial closure. The role of VAC technique in cases of severe diffuse peritonitis is not definitely incorrigible. However, in cases of severe complicated abdominal sepsis VAC come up as a last resort.
The electrocatalytic properties of carbon supported PtRu/C nanoalloys in oxidation of small organic molecules: Comparison with Pt/C catalyst
Lovi? Jelena D.,Popovi? Ksenija ?.,Tripkovi? Amalija V.
Hemijska Industrija , 2012, DOI: 10.2298/hemind111018104l
Abstract: The electrocatalytic activity of carbon supported PtRu/C catalysts, with different composition, toward the electrooxidation of methanol, CO and formic acid were examined in acid and alkaline solution at ambient temperature using thin-film rotating disk electrode (RDE) method and compared with activity of Pt/C. The catalysts were characterized by XRD, AFM and STM techniques. XRD pattern revealed that PtRu-1/C catalyst is consisted of two structures e.g. Pt-Ru-fcc and Ru-hcp (the solid solution of Ru in Pt and the small amount of Ru or solid solution of Pt in Ru), as opposed to PtRu-2/C catalyst which is consisted of one structure mostly, Pt-Ru-fcc. According to STM images, PtRu as well as Pt, particles size were between 2 and 6 nm, which is in a good agreement with the mean particles size determined by XRD. To establish the activity and stability of the catalysts potentiodynamic and quasi steady-state measurements were performed. It was found that the activity of Pt and PtRu for CO and methanol oxidation is a strong function of pH of solution. The kinetics are much higher in alkaline than in acid solution and the difference between Pt/C and PtRu/C is much less pronounced in alkaline media. Results presented in this work indicate that activity of PtRu catalysts depends on catalyst composition, e.g. on Pt/Ru atomic ratio, as well as on alloying degree of catalysts. Comparison of CO, methanol and formic acid oxidation on PtRu-2/C, PtRu-1/C and Pt/C catalysts revealed that PtRu-2/C is the most active one. It was shown that the PtRu-2/C catalyst, due to fact that it is consisted of only one phase, with high alloying degree, through the bifunctional mechanism improved by electronic effect, achieve the activity two times higher related to PtRu-1/C in the oxidation of all organic molecules investigated, and about three times higher compared to Pt/C in the oxidation of methanol and CO, and five times higher in formic acid oxidation.
Mathematical modelling of flux recovery during chemical cleaning of tubular membrane fouled with whey proteins
Luki? Nata?a Lj.,Popovi? Svetlana S.,Markovi? Jelena ?.
Acta Periodica Technologica , 2009, DOI: 10.2298/apt0940135l
Abstract: Membrane process efficiency in the dairy industry is impaired by the formation of deposits during filtration processes. This work describes cleaning procedures for ceramic tubular membrane (50 nm) fouled with whey proteins. Also, mathematical modelling was performed to obtain models which allow deeper insight into the mechanisms involved during cleaning procedures. The caustic solutions (0.2%w/w, 0.4%w/w and 1.0%w/w NaOH) and the mixture of two commercial detergents (0.8%w/w P3-ultrasil 69+0.5% w/w P3-ultrasil 67 and 1.2% P3-ultrasil 69+0.75 P3-ultrasil 67) were used as chemical cleaning agents. The results showed that the best flux recovery was achieved with 0.4%w/w NaOH solution. After analyzing the experimental data, five parameter and six parameter kinetic models were suggested for alkali and detergent cleaning, respectively. The changes of total and specific resistances, as well as the change of the effective pore diameter and deposit thickness during cleaning are estimated by applying these models.
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