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Search Results: 1 - 10 of 186633 matches for " Jefferson Costa de;Manno "
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Exigências de treonina digestível para leitoas mantidas em ambiente termoneutro dos 15 aos 30 kg
Saraiva, Edilson Paes;Oliveira, Rita Flávia Miranda de;Donzele, Juarez Lopes;Silva, Francisco Carlos de Oliveira;Siqueira, Jefferson Costa de;Manno, Maria Cristina;Oliveira, Will Pereira de;Nunes, Christiane Garcia Vilela;
Revista Brasileira de Zootecnia , 2007, DOI: 10.1590/S1516-35982007000800019
Abstract: this study was carried out to evaluate the requirements of digestible threonine in diets of gilts from 15 to 30kg, maintained in thermoneutral environment. seventy crossbreed gilts with an initial weight of 15.1 ± 0.4 kg were used in a randomized blocks design, with five treatments (levels of digestible threonine), seven replicates and two animals per experimental unity. the treatments corresponded to the levels of 0.54, 0.58, 0.61, 0.65, e 0.698% of digestible threonine. digestible threonine levels in the diet increased the daily weight gain in a quadratic way up to the level of 0.61% and the feed:gain ratio up to the level of 0.62%. protein and fat deposition rates also increased in a quadratic way reaching maximum value up to the level of 0.61%. a linear effect of the treatments was evidenced on the absolute and relative weights of the intestine. the calculated level of 0.62% of digestible threonine corresponding to a relation with digestible lysine of 67% and a daily intake of 7.11 g, provided better performance of gilts maintained in a thermoneutral environment from 15 to 30 kg.
Níveis de treonina digestível em ra??es para leitoas dos 15 aos 30 kg mantidas em ambiente de alta temperatura
Saraiva, Edilson Paes;Oliveira, Rita Flávia Miranda de;Donzele, Juarez Lopes;Silva, Francisco Carlos de Oliveira;Vaz, Roberta Gomes Mar?al Vieira;Siqueira, Jefferson Costa;Manno, Maria Cristina;Oliveira, Will Pereira de;
Revista Brasileira de Zootecnia , 2006, DOI: 10.1590/S1516-35982006000200021
Abstract: this trial was conducted to evaluate the dietary levels of digestible threonine for growing gilts on heat stress environment. seventy crossbreed gilts averaging initial weight of 14.9 ± 0.56 kg were assigned to a complete randomized blocks design with five treatments (levels of digestible threonine) of seven replications (two animals per experimental unity). the treatments consisted of the following dietary digestible threonine levels: 0.538, 0.577, 0.614, 0.651, and 0.688%. daily weight gain and threonine intake linearly increased as the dietary threonine level increased, whereas daily feed intake was not affected. although feed:gain ratio linearly changed with the treatments, the best dietary digestible threonine level was estimated in 0.587%. daily depositions of protein and fat in the carcass and absolute and relative weights of the evaluated organs were not affected by the treatments. it was concluded that gilts from 15 to 30 kg on heat stress environment require 0.587% of digestible threonine in the diet to obtain better feed:gain ratio, leading to a threonine digestible: digestible lysine ratio of 63%.
Compatibilidade vegetativa de nit-mutantes de Fusarium solani patogênicos e n?o-patogênicos ao feijoeiro e à soja
Oliveira, Virgínia C. de;Costa, Jefferson L. S. da;
Fitopatologia Brasileira , 2003, DOI: 10.1590/S0100-41582003000100013
Abstract: the purpose of this study was to determine the relatedness 18 isolates of fusarium solani f. sp. phaseoli and f. solani f. sp. glycines. the majority of these isolates have shown host un-especificity and were pathogenic to dry-bean (phaseolus vulgaris) and soybean (glycine max). the vegetative compatibility groups (vcg) of these isolates were determined. the nit-mutants (kclo3) of each isolate were paired on a nan03 minimal media to verify the heterocariosis formation. among the 18 isolates of f. solani, 13 were gathered in a single vcg. the nit-mutants strains of isolate f42, f. sp. phaseoli and isolate f46, f. sp. glycines were compatible to each other. three isolates consisted of single members of different vcgs and one of these isolates was not even incompatible with itself. vegetative compatibility, therefore, was found among isolates of f. solani f. sp. phaseoli and f. solani f. sp. glycines. this compatibility could be a reason for the existence of isolates pathogenic to both crops.
Análise de restri??o de DNA ribossomal amplificado (ARDRA) pode diferenciar Fusarium solani f. sp. phaseoli de F. solani f. sp. glycines
OLIVEIRA, VIRGíNIA C. de;COSTA, JEFFERSON L. S. da;
Fitopatologia Brasileira , 2002, DOI: 10.1590/S0100-41582002000600013
Abstract: molecular methods have been used to characterize diversity among pathogenic and non pathogenic isolates of fusarium spp. and to determine genetic relationships among formae speciales. pathogenicity tests performed on dry beans (phaseolus vulgaris) and soybeans (glycine max)with 17 isolates of fusarium solani did not demonstrate host specificity. amplified ribosomal dna restriction analysis (ardra) was used to analyze the its1 - 5,8s rdna - its2 region, amplified with primers its4 and its5. the amplified products were digested with the restriction enzymes hae iii and msp i. banding patterns generated by the enzyme hae iii enabled the differentiation of three groups within f. solani, one specific for isolates of f. solani f. sp. phaseoli and the other two for f. solani f. sp. glycines. the ardra technique, using the enzyme hae iii, is a promising marker to differentiate the formae specialesphaseoli from glycines within the f. solani complex.
Enhancement of soil DNA extraction by the use of a hand held mixer
Costa, Jefferson Luis da Silva;Oliveira, Virgínia Carla de;
Brazilian Journal of Microbiology , 2003, DOI: 10.1590/S1517-83822003000400004
Abstract: the efficiency of soil dna extraction using a bead beater homogenizer (biospec products - germany) was compared to that obtained with a hand held mixer (moulinex - brazil). the hand held mixer costs 100 times less and extracted seven times more crude dna than the bead beater.
Compatibilidade vegetativa de nit-mutantes de Fusarium solani patogênicos e n o-patogênicos ao feijoeiro e à soja
Oliveira Virgínia C. de,Costa Jefferson L. S. da
Fitopatologia Brasileira , 2003,
Abstract: Este trabalho objetivou determinar o grau de relacionamento entre 18 isolados de Fusarium solani f. sp. phaseoli e F. solani f. sp. glycines. A maioria destes isolados (14) demonstrou inespecificidade de hospedeiro sendo patogênicos ao feijoeiro (Phaseolus vulgaris) e a soja (Glycine max). Grupos de compatibilidade vegetativa de nit-mutantes destes isolados foram ent o determinados. Utilizou-se, como indutor de muta o, o clorato de potássio (KClO3), sendo estes nit-mutantes justapostos sobre meio mínimo contendo NAN0(3) para verificar a forma o de heterocariose. Dos 18 isolados de F. solani, 13 foram reunidos em um único GCV. Neste grupo os isolados nit-mutantes F42, f. sp. phaseoli e F46, f. sp. glycines, foram compatíveis entre si. Três isolados constituíram em membros únicos de GCVs diferentes, sendo um destes isolados considerado auto-incompatível. Encontrou-se, portanto, compatibilidade vegetativa entre isolados de F. solani f. sp. phaseoli e F. solani f. sp. glycines. Esta compatibilidade pode ser uma justificativa para existência de isolados patogênicos a ambas culturas.
Análise de restri o de DNA ribossomal amplificado (ARDRA) pode diferenciar Fusarium solani f. sp. phaseoli de F. solani f. sp. glycines
OLIVEIRA VIRGíNIA C. de,COSTA JEFFERSON L. S. da
Fitopatologia Brasileira , 2002,
Abstract: Métodos moleculares têm sido utilizados para caracterizar a diversidade entre isolados de Fusarium spp. patogênicos e n o patogênicos a uma cultura e, para determinar rela es genéticas entre formae speciales. Testes de patogenicidade realizados em soja (Glycine max) e feijoeiro (Phaseolus vulgaris) com 17 isolados de Fusarium solani n o demonstraram especificidade de hospedeiros. Utilizou-se a técnica ARDRA (Amplified Ribosomal DNA Restriction Analysis) para analisar a regi o ITS1 - 5,8S rDNA - ITS2, amplificada com os primers ITS5 e ITS4. Os produtos amplificados foram digeridos com as enzimas de restri o Hae III e Msp I. Os padr es de bandas gerados pela digest o com a enzima Hae III permitiram diferenciar três grupos entre os isolados de F. solani, sendo um grupo específico para isolados de F. solani f. sp. phaseoli com 100% de similaridade entre os 11 isolados. Entre os isolados de F. solani f. sp glycines foram observados dois padr es distintos de restri o. A técnica de ARDRA utilizando a enzima Hae III apresenta, portanto, potencial para utiliza o como um marcador para diferencia o entre as formae specialesphaseoli e glycines, dentro do complexo F. solani.
MéTODOS DE INOCULA O DE PL NTULAS DE FEIJOEIRO PARA AVALIA O DE GERMOPLASMA QUANTO A RESISTêNCIA A Sclerotinia sclerotiorum (Lib.) De Bary1 INOCULATION METHODS OF COMMON BEAN SEEDLINGS FOR EVALUATION OF GERMOPLASM RESISTANCE TO Sclerotinia sclerotiorum (Lib.) De Bary
Eliane Divina de Tolêdo-Souza,Jefferson Luis da Silva Costa
Pesquisa Agropecuária Tropical , 2007, DOI: 10.5216/pat.v33i2.2348
Abstract: O desenvolvimento de uma metodologia adequada à inocula o de plantas do feijoeiro com Sclerotinia sclerotiorum (Lib.) De Bary é de grande importancia para avalia o de genótipos quanto à resistência ao mofo branco. O objetivo deste trabalho foi testar a eficiência de três métodos de inocula o em diferentes partes da plantula de feijoeiro aos onze dias após a emergência. Uma suspens o de ascósporos, inoculada em plantas em estágio de flora o, foi utilizada para validar os métodos testados. Os métodos constituíram da inocula o de folhas ou axilas foliares com discos de BDA contendo micélio do fungo e inocula o das hastes com palito colonizado pelo patógeno. Nove genótipos do gênero Phaseolus spp. e dois isolados de S. sclerotiorum (UnB 1.541 e UnB 1.547) foram utilizados para comparar os métodos. Após a inocula o, as plantas permaneceram em camara de nevoeiro, com umidade aproximada de 100%, temperatura de 21±2oC e fotoperíodo de 12 horas de luz/ 12 horas de escuro. Permaneceram, ent o, dois dias para o método de inocula o das folhas com discos de BDA, quatro dias para o método de inocula o das axilas, e dez dias para os métodos de inocula o das hastes com palitos colonizados pelo patógeno e inocula o das flores com ascosporos. Após os períodos determinados para cada método, as avalia es foram realizadas, utilizando-se uma escala de notas variando de 1 a 9 (1= ausência de sintomas e 9= morte da planta). O método de inocula o nas axilas das plantulas, com discos de BDA contendo micélio do fungo, discriminou melhor os genótipos, apresentando resultados similares à inocula o de flores com ascósporos. O isolado UnB 1.541 apresentou maior agressividade e discriminou melhor os genótipos quanto a sua resistência ou suscetibilidade a S. Sclerotiorum. PALAVRAS-CHAVE: Mofo branco; resistência genética; Phaseolus spp; método de inocula o. The development of a reliable method of inoculation of dry bean (Phaseolus spp.) with Sclerotinia sclerotiorum is of great importance for evaluation of genotypes for resistance to white mold. The objective of this study was to test three inoculation methods in different parts of 11-day-old Phaseolus spp seedlings. The plant was inoculated with a suspension of ascospores at the blossom stage as a reference for comparing the efficiency of the methods tested, since it simulates field infections. The methods consisted of the inoculation of leaves or axils with disks of PDA containing mycelia; and inoculation of the stems with a toothpick colonized by the pathogen. Nine dry bean genotypes of Phaseolus spp. and two isolates
Shoot tip and cotyledon explants of Eucalyptus saligna Sm. cultivated on different kanamycin levels
André Luís Lopes da Silva,Yohana de Oliveira,Jefferson da Luz Costa,Elisangela Masetto
Journal of Biotechnology and Biodiversity , 2010,
Abstract: An efficient selective agent helps to obtain transgenic plants; the most used selective agent for genetic transformation of Eucalypt is kanamycin. The aim of this research was to determine ideal dose of kanamycin in shoot tip and cotyledon explants of E. saligna for use as selective agent in genetic transformation. Two experiments were carried out, first in order to test effects of the kanamycin in cotyledons and second, in order to test effects of the kanamycin in shoot tip. In the cotyledons experiment were tested 0, 12.5, 25, 37.5 and 50 mg.L-1 kanamycin on a callus formation medium and for shoot tip were tested 50, 75, 100, 125 and 150 mg.L-1 kanamycin on a multiplication medium. Cotyledons can be selected more quickly than shoot tip, moreover needs lower kanamycin levels than shoot tip, what results more speed and economy during genetic transformation process. It is probable that levels lower than 12.5 mg.L-1 kanamycin must be ideal for the cotyledons selection and 50 mg.L-1 kanamycin for shoot tip.
Hydroponics growth of Eucalyptus saligna Sm. on salt-stress mediated by sodium chloride
André Luís Lopes da Silva,Yohana de Oliveira,Roberson Dibax,Jefferson da Luz Costa
Journal of Biotechnology and Biodiversity , 2012,
Abstract: The aim of this work was to evaluate the growth of two clones of Eucalyptus saligna on salt-stress mediated by NaCl in hydroponics. Micropropagated plants of the clones p0 and p1 were acclimatizated and cultivated in hydroponics at 0 and 300 mM NaCl levels. The total length, volume, number, fresh mass and dry mass of the roots, the height,fresh and dry mass of the aerial part and the fresh and dry mass of the complete plant were evaluated to the 14 days of hydroponic culture with NaCl. There were significant differences among the clones. The clone p0 wassuperior to the clone p1 in relation to volume of the roots, root number, root fresh mass and total fresh mass of theroot. Regards the effect of the salinity on the plants, significant reduction was observed in the height of the aerial part, fresh mass of the aerial part and the total fresh mass. Even so, the interaction between the clones and the concentrations of NaCl was significant for the total fresh mass. In the period of 14 days of hydroponic culture on 300 mM NaCl was possible to discriminate these two clones in relation to the tolerance and susceptibility to the salt stress. The clone p0 presented higher growth and larger tolerance to the salinity than clone p1.
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