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Search Results: 1 - 10 of 2304 matches for " Jana Auer "
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Zona pellucida from fertilised human oocytes induces a voltage-dependent calcium influx and the acrosome reaction in spermatozoa, but cannot be penetrated by sperm
Catherine Patrat, Jana Auer, Patricia Fauque, Roger L Leandri, Pierre Jouannet, Catherine Serres
BMC Developmental Biology , 2006, DOI: 10.1186/1471-213x-6-59
Abstract: The hZP of fertilised oocytes retained their ability to bind sperm (albeit less strongly than that from unfertilised oocytes), to induce an intraspermatic calcium influx through voltage-dependent channels similar to that observed with hZP from unfertilised oocytes and to promote the acrosome reaction at a rate similar to that induced by the ZP of unfertilised oocytes (61.6 ± 6.2% vs60.7 ± 9.1% respectively). Conversely, the rate of hZP penetrated by sperm was much lower for fertilised than for unfertilised oocytes (19% vs 57% respectively, p < 0.01). We investigated the status of ZP2 in the oocytes used in the functional tests, and demonstrated that sperm binding and acrosome reaction induction, but not ZP penetration, occurred whether or not ZP2 was cleaved.The change in ZP function induced by fertilisation could be different in human and mouse species. Our results suggest a zona blocking to polyspermy based at the sperm penetration level in humans.Sperm interaction with the extracellular matrix surrounding the oocyte, the zona pellucida (ZP), is a key step in mammalian fertilisation. The structure and function of the ZP were first studied in mice in the 1980s [1-3]. Mouse ZP contains three glycoproteins: ZP1, ZP2 and ZP3. ZP3 binds to primary receptors on capacitated spermatozoa, inducing a cascade of intraspermatic events including the biphasic calcium influx leading to the acrosome reaction (AR). Following the AR, spermatozoa remain bound to the ZP via ZP2; they then penetrate the ZP and fuse with the egg plasma membrane [4]. The fertilisation is followed by the fusion of peripheral cortical granules with the vitelline membrane, resulting in the discharge of the contents of these granules into the perivitelline space. This exocytosis modifies the ZP matrix such that sperm no longer bind to or penetrate ZP, preventing polyspermy.Our understanding at a molecular level of gamete interaction in humans is much more restricted than for mice and other mammalian species
Fidgetin-Like1 Is a Strong Candidate for a Dynamic Impairment of Male Meiosis Leading to Reduced Testis Weight in Mice
David L'H?te,Magalie Vatin,Jana Auer,Johan Castille,Bruno Passet,Xavier Montagutelli,Catherine Serres,Daniel Vaiman
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0027582
Abstract: In a previous work, using an interspecific recombinant congenic mouse model, we reported a genomic region of 23 Mb on mouse chromosome 11 implicated in testis weight decrease and moderate teratozoospermia (~20–30%), a Quantitative Trait Locus (QTL) called Ltw1. The objective of the present study is to identify the gene underlying this phenotype.
Cholesterol Depletion Disorganizes Oocyte Membrane Rafts Altering Mouse Fertilization
Jorgelina Buschiazzo, Come Ialy-Radio, Jana Auer, Jean-Philippe Wolf, Catherine Serres, Brigitte Lefèvre, Ahmed Ziyyat
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0062919
Abstract: Drastic membrane reorganization occurs when mammalian sperm binds to and fuses with the oocyte membrane. Two oocyte protein families are essential for fertilization, tetraspanins and glycosylphosphatidylinositol-anchored proteins. The firsts are associated to tetraspanin-enriched microdomains and the seconds to lipid rafts. Here we report membrane raft involvement in mouse fertilization assessed by cholesterol modulation using methyl-β-cyclodextrin. Cholesterol removal induced: (1) a decrease of the fertilization rate and index; and (2) a delay in the extrusion of the second polar body. Cholesterol repletion recovered the fertilization ability of cholesterol-depleted oocytes, indicating reversibility of these effects. In vivo time-lapse analyses using fluorescent cholesterol permitted to identify the time-point at which the probe is mainly located at the plasma membrane enabling the estimation of the extent of the cholesterol depletion. We confirmed that the mouse oocyte is rich in rafts according to the presence of the raft marker lipid, ganglioside GM1 on the membrane of living oocytes and we identified the coexistence of two types of microdomains, planar rafts and caveolae-like structures, by terms of two differential rafts markers, flotillin-2 and caveolin-1, respectively. Moreover, this is the first report that shows characteristic caveolae-like invaginations in the mouse oocyte identified by electron microscopy. Raft disruption by cholesterol depletion disturbed the subcellular localization of the signal molecule c-Src and the inhibition of Src kinase proteins prevented second polar body extrusion, consistent with a role of Src-related kinases in fertilization via signaling complexes. Our data highlight the functional importance of intact membrane rafts for mouse fertilization and its dependence on cholesterol.
The integration of functional genomics, combinatorial chemistry and nanotechnology into a miniaturized drug discovery process
M Auer
Arthritis Research & Therapy , 2001, DOI: 10.1186/ar153
Abstract: Single molecule fluorescence spectroscopy and confocal nanoscanning/AIDA technology provide an optimal combination for a miniaturized automated uHTS process with high mechanistic resolution in functional and coupled assay systems.
Short Note on Correlation in a Hull-White Tree
Martin Auer
Lecture Notes in Engineering and Computer Science , 2009,
Abstract:
Entre assimilation et exclusion: La représentation du Highlander dans la presse des Basses Terres d’Ecosse au milieu du dix-neuvième siècle
Christian Auer
Synergies Royaume-Uni et Irlande , 2008,
Abstract: Au cours des premières décennies du dix-neuvième siècle, la paysannerie des Hautes Terres d’Ecosse connut une profonde et douloureuse mutation sous l’impulsion de divers facteurs culturels et économiques. La presse des Basses Terres se fit l’écho de ces transformations et se montra le plus souvent critique envers les habitants d’une région per ue comme étrangère. L’analyse du discours du Blackwood’s Edinburgh Magazine, l’une des publications littéraires majeures en Grande-Bretagne, et de deux des journaux qui firent preuve de la plus grande virulence à l’encontre des Highlanders, le Perthshire Advertiser et le Fifeshire Journal, permet d’identifier la nature des stratégies de distanciation auxquelles la presse des Basses Terres eut recours. Dans cet article je m’efforcerai de démontrer que les analyses proposées par Edward Said dans Orientalism et Culture and Imperialism peuvent s’appliquer à la description des mécanismes mis en uvre par la presse des Lowlands.Said, en effet, assimile le discours colonial à un système statique d’essentialisme synchronique . Or le discours de la presse des Lowlands avait comme vocation première de présenter la communauté des Highlands comme essentiellement statique, de la rejeter dans l’altérité et d’en discréditer les schémas culturels. La perception fondamentalement négative de la celtitude s’articulait autour d’un ensemble de couples antithétiques (travail-indolence, supériorité-infériorité, modernité-archa sme ou encore civilisationbarbarie). Une démarche de type ethnocidaire s’imposait donc afin que le Highlander puisse quitter son statut d’étranger pour bénéficier, au même titre que les autres membres de la communauté britannique, des bienfaits de la civilisation et du progrès.
Klinische Studie/Klinische Praxis: Kann AT-I-Rezeprotblockade das Risiko für Restenose nach Implantation von "Bare-metal" Stents in Koronararterien reduzieren?
Auer J
Zeitschrift für Gef??medizin , 2009,
Abstract:
Update: Therapie der chronischen Herzinsuffizienz
Auer J
Journal für Kardiologie , 2009,
Abstract: Die Herzinsuffizienz stellt eine der h ufigsten internistischen Erkrankungen dar. Grundpfeiler der pharmakologischen Therapie der chronischen Herzinsuffizienz mit dem Ziel einer Prognoseverbesserung ist die Blockade der im Rahmen des Syndroms Herzinsuffizienz stattfindenden neurohumoralen Aktivierung. Die Ger tetherapie (CRT, ICD) gewinnt im Therapiekonzept der Behandlung von Patienten mit chronischer Herzinsuffizienz zunehmende Bedeutung. Effektive Therapien etwa für spezifische Behandlung der diastolischen Herzinsuffizienz sind limitiert. Interessante neue Therapieans tze zur Behandlung der chronischen Herzinsuffizienz werden gegenw rtig in klinischen Studien untersucht. Gro e klinische Untersuchungen mit neuen Substanzen brachten zum Teil gemischte Ergebnisse. Das unterstreicht die Tatsache, dass es schwierig ist, zus tzlich zur momentan verfügbaren Therapie einen additiven Benefit zu erreichen. Die folgende Arbeit gibt eine übersicht, was sich auf dem Gebiet der Herzinsuffizienztherapie in den vergangenen Jahren entwickelt hat.
Klinische Studien/Klinische Praxis: Kann AT-I-Rezeptorblockade das Risiko für Restenose nach Implantation von "Bare-metal" Stents in Koronararterien reduzieren!
Auer J
Journal für Kardiologie , 2009,
Abstract:
Aus dem Herzkatheterlabor: Transientes linksventrikul res "Apical Ballooning" als Differentialdiagnose zum akuten Myokardinfarkt
Auer J
Journal für Kardiologie , 2004,
Abstract:
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