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Fatores associados à soroprevalência de Salmonella em rebanhos comerciais de suínos
Kich Jalusa Deon,Mores Nelson,Piffer Itamar Antonio,Coldebella Arlei
Ciência Rural , 2005,
Otimiza??o da técnica da PCR para a detec??o de Actinobacillus pleuropneumoniae
Souza, Karina Koerich de;Klein, Catia Silene;Kich, Jalusa Deon;Coldebella, Arlei;Alberton, Geraldo Camilo;
Ciência Rural , 2008, DOI: 10.1590/S0103-84782008000800023
Abstract: the use of molecular methods based on pcr is important in actinobacillus pleuropneumoniae detection, being able to identify the infection before the establishment of the disease in the herd. these methods have larger sensitivity when compared with traditional methods of bacteriological isolation, but they can suffer influence of substances that reduce the specificity of the test and resulting in inespecific amplifications. in order to reduce inespecific amplifications, observed when applied the pcr technique for the gene cpx in tonsil's tissue samples, the optimization was performed, in which different annealing temperatures were analyzed and introduced, in the technique, an antibody that binds to the enzyme taq dna polimerase, increasing its specificity. in parallel, an experiment was performed in order to verify the inhibiting effect of the tonsil's tissue on the pcr results. for that, portions of tonsil from animals negative to the a. pleuropneumoniae were artificially contaminated with the reference sample of the sorotype 5b. the addition antibody for the enzyme taq dna polimerase and the increase of the primers anneling temperature to 57°c reduced the inespecific amplifications. the results obtained in the experiment demonstrated a possible inhibiting effect of the tonsil's tissue in the pcr amplifications. besides, amplifications depend on at least 675 ufc present in the aliquot of samples that will be used in pcr (equivalent to 1.35 x 105 ufc ml-1), therefore, samples tissue's fragments in initial infections and/or with few cells can result in false-negative.
Fatores associados à soroprevalência de Salmonella em rebanhos comerciais de suínos
Kich, Jalusa Deon;Mores, Nelson;Piffer, Itamar Antonio;Coldebella, Arlei;Amaral, Armando;Ramminger, Lucas;Cardoso, Marisa;
Ciência Rural , 2005, DOI: 10.1590/S0103-84782005000200024
Abstract: a cross-sectional study was conducted with 65 finishing pig herds from the states of rio grande do sul and santa catarina, brazil, in order to identify factors associated with seroprevalence for salmonella. pig farms were visited one week prior to slaughtering of animals when personnel were asked to answer a questionnaire. feed was also sampled for attempts of salmonella isolation, water for colimetrics analisis and around 40 pigs were bled. feed samples were subjected to salmonella isolation in selective media and sera were tested in an elisa, plates were coated with antigens of serovar typhimurium. herds were classified in one of three categories according to the prevalence of elisa positive sera, being low (less then 40% of positive sera), medium (between 40 and 70% positive sera) or high (more then 70% positive sera). seroprevalence was used as the explanatory variable and results obtained from the attempts to isolate salmonella from feed, water colimetrics results and the questionnaire answers were used as explanatory variables. initially, attempts of association between explanatory and explained variables were performed using the chi-square test. when associated (p£0.1), the two variables underwent multiple correspondence analysis. factors associated with herds having high seroprevalence were: in finishing herds, pelleted feed, swine manure disposal less than 100m from surface water, feeder not provided with water drinker, swine from several herds transported together to slaughterhouse; in the farrow-to-finish herds, feed ingridients exposure to other animals, no active rodent control, dry feed, absence of fence, whitewashing of facilities after cleaning and disinfecting and permission for other people entrance to the herd. among the 65 pig herds visited, 98.5% were elisa-positive, with seroprevalence of 57.6% (confidence interval 56 to 60%).
Rea??o em Cadeia da Polimerase (PCR) baseada no gene cpx para detec??o de Actinobacillus pleuropneumoniae em suínos natural e experimentalmente infectados
Souza, Karina Koerich de;Klein, Catia Silene;Kich, Jalusa Deon;Coldebella, Arlei;Alberton, Geraldo Camilo;
Ciência Rural , 2008, DOI: 10.1590/S0103-84782008000700024
Abstract: swine pleuropneumonia is one of the most important pig respiratory diseases and has been found in all producer countries. for control and monitoring of pleuropneumonia, it is necessary the development of fast and specific methods of diagnosis. to validate pcr based on the cpx gene of actinobacillus pleuropneumoniae in positive pigs, an experimental infection with a. pleuropneumoniae serotype 5b was performed and samples were obtained by tonsil swab, tonsil biopsy and blood for pcr, bacterial isolation and elisa, respectively. these tests were then performed in naturally infected pigs from three herds with different sanitary situations of clinical disease. in each herd, five groups of different ages were analyzed. tonsil biopsy for bacterial isolation and pcr and blood to determine the herd serological status was collected. the results obtained in the experimental infection confirmed that, even with the infection establishment, proved with bacterial isolation, it was not possible to detect the agent by pcr 45 days after infection. in naturally infected animals, pcr was more sensitive than bacterial isolation. the association between pcr and elisa is a good alternative to define the herd sanitary status regarding the infection with a. pleuropneumoniae.
Efeito de probiótico na infec??o e excre??o fecal de Salmonella em suínos
Nogueira, Mariana Gomes;Calveyra, Juliana Cafruni;Kich, Jalusa Deon;Coldebella, Arlei;Mores, Nelson;Cardoso, Marisa Ribeiro de Itapema;
Ciência Rural , 2012, DOI: 10.1590/S0103-84782012000300021
Abstract: control of salmonella transmission has been a challenge for the pork production companies. the aim of this study was to evaluate the effect of oral administration of probiotics on the occurrence of infection and fecal excretion of salmonella in growers. the treatments consisted of basal diet without additives (control) or added of probiotic (107cfu g-1 of viable cells of the genera bifidobacterium, enterococcus, lactobacillus and saccharomyces). six 50 days-old pigs were allocated into each treatment, with two replicates per treatment. all animals were inoculated with salmonella typhimurium (106cfu ml-1) after 14 days of housing. afterwards, blood and feces samples were taken weekly and on day 35 post-inoculation the animals were euthanized and necropsied. the animals in both treatment groups were infected by salmonella and seroconverted. there was no difference (p>0.05) between groups in mean counts of salmonella, enterococcus, lactobacillus and coliforms in the feces samples, but the probiotic administration resulted in a lower frequency of isolation of salmonella from liver (p=0.04), mesenteric lymph nodes (p=0.04), lung (p=0.03) and spleen (p=0.01). it was concluded that the probiotic microorganisms tested in this study were not able to protect against the infection or to decrease the fecal excretion of salmonella in growing pigs, but were able to decrease the number of carriers in the mesenteric lymph nodes.
Efeito do manejo pré-abate sobre alguns parametros fisiológicos em fêmeas suínas pesadas
Dalla Costa, Osmar Antonio;Ludke, Jorge Vitor;Coldebella, Arlei;Kich, Jalusa Deon;Costa, Mateus José Rodrigues Paranhos da;Faucitano, Luigi;Peloso, José Vicente;Dalla Roza, Darlan;
Ciência Rural , 2009, DOI: 10.1590/S0103-84782009000300033
Abstract: the aim of the research was to evaluate the effect of pig lairage time (pdf=3, 5, 7 and 9 hours) and evaluate the effect of pig position into the truck (pbo) during transportation to slaughterhouse, in winter or summer conditions, on some physiologic parameters evaluated on 64 heavyweight females with mean liveweight of 130kg during pre-slaughter events. the following effects were considered in the statistical analysis of variance model: block (bl=summer farm or winter farm), pdf, pbo and interaction (block x pdf), under other factors. the pdf influenced significativelly blood lactate and saliva cortisol levels. pig submitted to 5 and 7 hours of lairage had higher levels of lactate when compared to pigs submitted to 3 and 9 hours of lairage. during transport the heart rate were higher than in other pre-slaughter events. it is concluded that increasing pdf above 3 hours had no effects on heart frequency, glucose and cpk levels and salivary cortisol levels but affects the blood lactate levels.
Tempo de jejum na granja sobre o perfil hormonal e os parametros fisiológicos em suínos de abate pesados
Dalla Costa, Osmar Antonio;Ludke, Jorge Vitor;Costa, Mateus José Rodrigues Paranhos da;Faucitano, Luigi;Coldebella, Arlei;Kich, Jalusa Deon;Peloso, José Vicente;Dalla Roza, Darlan;
Ciência Rural , 2008, DOI: 10.1590/S0103-84782008000800032
Abstract: the aim of this study was to evaluate the effect of fasting time on the farm (tjg) and the position of the animals on truck compartment during transport to slaughterhouse in the hormonal and physiological status of heavy-weight swine in order to obtain future management improvements and to reduce meat quality downgrades. sixty-four females obtained from two farms and averaging 133+11kg body weight were used. fasting time evaluated on farms were 9, 12, 15 and 18 h while the positions considered in the truck were box (front, middle, and back), deck (under and upper level) e side (right and left). swine blood concentration of glucose, lactate and cpk were evaluated at slaughter. cortisol concentration on saliva (ccs) was evaluated on the farm (24 hours before uploading and after loading) and at slaughterhouse (after downloading and before slaughter). hearth frequency was continuously evaluated during pre-slaughter management. interaction between tjg and moment of salivary cortisol sampling and heart rate data recording were observed. ccs and heart rate values increased from farm to truck downloading at slaughterhouse, while pre-slaughter resting reduced (p<0.05) the values. the ccs values ranged in a gradient fashion in function of tjg and moment of salivary cortisol sampling in the following manner: pigs submitted to 18 hours of fasting showed smallest changes (p<0.05) of ccs during the pre-slaughter management when compared with ccs of pigs submitted to lower fasting times and, between those, the animals with 9 hours of fasting showed the greatest (p<0.05) variations. before slaughter swine submitted to 9 hours of fasting at farm presented the highest values (p<0.05) of ccs when compared with the other fasting times evaluated. it is concluded that pre-slaughter management conditions affect (p<0.05) the salivary cortisol levels and heart rate but the evaluated tjg had no effects (p>0.05) on glucose, lactate and cpk levels at slaughter time.
Tempo de jejum dos suínos no manejo pré-abate sobre a perda de peso corporal, o peso do conteúdo estomacal e a incidência de úlcera esofágica-gástrica
Dalla Costa, Osmar Antonio;Costa, Mateus José Rodrigues Paranhos da;Ludke, Jorge Vitor;Coldebella, Arlei;Kich, Jalusa Deon;Peloso, José Vicente;Faucitano, Luigi;Dalla Roza, Darlan;
Ciência Rural , 2008, DOI: 10.1590/S0103-84782008000100032
Abstract: the objective of this study was to evaluate the effect of fasting time period (9, 12, 15 and 18 hours) at the farm (tjg) and the pig?s position in the lorry?s livestock compartment (pbo) during the transport from the farm to the processing plant on: body weight loss (ppc), weight of stomach (full= pec and empty= pev), weight of stomach contents (pce), and incidence of gastric ulcer (elg). a total of 192 females with average 34.51±11.80kg of body weigh were used. the statistical model considered the effects of: block (season, bl), tjg, pbo and the interaction between bl and tjg. there was a significant effect of tjg only on stomach content. there was no effect of pbo on any of the evaluated variables. a pec inferior to 500g was observed in 90.3% of the animals, while 8.56% of the pigs presented pec between 500 and 800g. incidence of elg was low (14.97%), and among the elg animals, 13.90% showed grade 1 lesion, and 1.09% grade two lesion. as a conclusion, pigs submitted to a fasting period of 15 hours have less stomach contents at the processing.
Monika Müller,Patrícia Schwarz,Jalusa Deon Kich,Marisa Ribeiro de Itapema Cardoso
Ciência Animal Brasileira , 2009,
Abstract: Estudos que elucidem a cadeia de transmiss o de Salmonella enterica nos sistemas de produ o de suínos s o importantes para que seja possível implementar programas de controle da infec o. O objetivo deste estudo foi comparar o índice de animais positivos para Salmonella sp. no início da fase de termina o e ao abate e identificar possíveis fontes de contamina o no período. Em três granjas terminadoras, coletaram-se: suabes de superfície nas baias e nos silos durante o vazio sanitário; amostras de fezes e sangue dos animais no dia do alojamento; alíquotas de todos os lotes de ra o e amostras de sangue, linfonodos mesentéricos (LM) e conteúdo intestinal (CI) ao abate. As amostras de sangue foram submetidas a teste de ELISA-LPS para Salmonella Typhimurium. Nas demais amostras, pesquisou-se a presen a de Salmonella sp. As amostras de ra o foram adicionalmente submetidas à técnica da Rea o em Cadeia da Polimerase (PCR) amplificando o gene invA. Todos os animais foram negativos para presen a de Salmonella sp. nas fezes no início da termina o; entretanto, em duas granjas havia animais soropositivos (12% e 28%, respectivamente). Em duas granjas havia contamina o residual no ambiente e na terceira granja, em um dos lotes de ra o, detectou-se a presen a de Salmonella sp. pela PCR. Ao abate, acima de 90% dos animais foram positivos no teste de ELISA-LPS, sendo que em todos os lotes encontrou-se um número variável (12-92%) de portadores em LM e CI. A partir disso, concluiu-se que a termina o foi a fase crítica para a amplifica o da infec o por Salmonella sp., sendo a presen a residual do microrganismo na granja e o fornecimento de ra o contaminada fontes prováveis de infec o. PALAVRAS-CHAVES: Abate, isolamento, Salmonella, sorologia, suíno, termina o. Studies assessing the Salmonella transmission chain in pig herds are the first step to start a control program. The aims of this study were to compare the prevalence of Salmonella positive pigs at the beginning of the finishing phase and at slaughter, and to identify the possible sources of contamination in the farms. In three finishing farms, environmental swabs from the barns and from the feed silos were collected during the sanitary emptiness. Furthermore, samples of feces and blood from the animals on the day of housing; and aliquots from all feed lots were taken. At slaughter, blood, mesenteric lymph nodes (LM) and intestinal content (CI) were sampled. Blood samples were submitted to a S. Typhimurium ELISA-LPS test. All other samples were submitted to a Salmonella isolation protocol. Feed samples we
Prevalência de Salmonella sp. em suínos abatidos no Estado de Mato Grosso
Silva, Maria Cristina da;Faria, Givago Silva;Paula, Daphine Ariadne Jesus de;Martins, Rodrigo Prado;Caramori Junior, Jo?o Garcia;Kich, Jalusa Deon;Colodel, Edson Moleta;Nakazato, Luciano;Dutra, Valéria;
Ciência Rural , 2009, DOI: 10.1590/S0103-84782008005000035
Abstract: the present study aims to determine the prevalence of salmonella sp. in pigs in slaughterhouses under federal inspection at mato grosso, brazil. samples of mesenteric lymph nodes and tonsils were collected in three abattoirs from different regions. three hundred samples were processed; fifty (16.6%) were positive for salmonella sp. and belonged to fourteen different serological types. the most common serotypes were derby (16%), typhimurium (14%), london (12%) and give (12%). the epidemiological results obtained show a low prevalence of this bacterium when compared to studies in other brazilian states.
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