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Search Results: 1 - 10 of 19607 matches for " Jae Yun Lim "
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Effects of Dietary Isoflavones from Puerariae Radix on Lipid and Bone Metabolism in Ovariectomized Rats
Dong Wook Lim,Jae Goo Kim,Yun Tai Kim
Nutrients , 2013, DOI: 10.3390/nu5072734
Abstract: Puerariae radix, the dried root of Pueraria lobata Ohwi, is one of the earliest and most important edible crude herbs used for various medical purposes in oriental medicine. This study evaluated the metabolic effects of total isoflavones from P. lobata (PTIF) in ovariectomized (OVX) rats. The OVX rats were divided into four groups treated with distilled water, 17β-estradiol (E2 10 μg/kg, once daily, i.p.) and PTIF (30 and 100 mg/kg, once daily, p.o.) for eight weeks. The treatments with high-dose PTIF significantly decreased the bone mineral density (BMD) loss in the femur and inhibited the increase in body weight and lipoprotein levels compared to the OVX-control group without elevating the serum levels of the liver enzymes, aspartate aminotransferase (AST) and alanine transaminase (ALT). Furthermore, PTIF exhibits a hepatoprotective effect in OVX-induced hepatic steatosis, indicated with reduced hepatic lipid contents. Taken together, our findings suggest that PTIF may be useful for controlling lipid and bone metabolism, at least in OVX rats. Further research is necessary to determine whether PTIF will have the same effects in humans.
Edge detection based on morphological amoebas
Won Yeol Lee,Young Woo Kim,Se Yun Kim,Jae Young Lim,Dong Hoon Lim
Computer Science , 2011, DOI: 10.1179/1743131X11Y.0000000013
Abstract: Detecting the edges of objects within images is critical for quality image processing. We present an edge-detecting technique that uses morphological amoebas that adjust their shape based on variation in image contours. We evaluate the method both quantitatively and qualitatively for edge detection of images, and compare it to classic morphological methods. Our amoeba-based edge-detection system performed better than the classic edge detectors.
Overexpression of the M2 isoform of pyruvate kinase is an adverse prognostic factor for signet ring cell gastric cancer
Jae Yun Lim,Sun Och Yoon,So Young Seol,Soon Won Hong
World Journal of Gastroenterology , 2012, DOI: 10.3748/wjg.v18.i30.4037
Abstract: AIM: To investigate M2 isoform of pyruvate kinase (PKM2) expression in gastric cancers and evaluate its potential as a prognostic biomarker and an anticancer target. METHODS: All tissue samples were derived from gastric cancer patients underwent curative gastrectomy as a primary treatment. Clinical and pathological information were obtained from the medical records. Gene expression microarray data from 60 cancer and 19 non-cancer gastric tissues were analyzed to evaluate the expression level of PKM2 mRNA. Tissue microarrays were constructed from 368 gastric cancer patients. Immunohistochemistry was used to measure PKM2 expression and PKM2 positivity of cancer was determined by proportion of PKM2-positive tumor cells and staining intensity. Association between PKM2 expression and the clinicopathological factors was evaluated and the correlation between PKM2 and cancer prognosis was evaluated. RESULTS: PKM2 mRNA levels were increased more than 2-fold in primary gastric cancers compared to adjacent normal tissues from the same patients (log transformed expression level: 7.6 ± 0.65 vs 6.3 ± 0.51, P < 0.001). Moreover, differentiated type cancers had significantly higher PKM2 mRNA compared to undifferentiated type cancers (log transformed expression level: 7.8 ± 0.70 vs 6.7 ± 0.71, P < 0.001). PKM2 protein was mainly localized in the cytoplasm of primary cancer cells and detected in 144 of 368 (39.1%) human gastric cancer cases. PKM2 expression was not related with stage (P = 0.811), but strongly correlated with gastric cancer differentiation (P < 0.001). Differentiated type cancers expressed more PKM2 protein than did the undifferentiated ones. Well differentiated adenocarcinoma showed 63.6% PKM2-positive cells; in contrast, signet-ring cell cancers showed only 17.7% PKM2-positive cells. Importantly, PKM2 expression was correlated with shorter overall survival (P < 0.05) independent of stage only in signet-ring cell cancers. CONCLUSION: PKM2 expression might be an adverse prognostic factor for signet-ring cell carcinomas. Its function and potential as a prognostic marker should be further verified in gastric cancer.
Thioredoxin and thioredoxin-interacting protein as prognostic markers for gastric cancer recurrence
Jae Yun Lim,Sun Och Yoon,Soon Won Hong,Jong Won Kim
World Journal of Gastroenterology , 2012, DOI: 10.3748/wjg.v18.i39.5581
Abstract: AIM: To evaluate the potential of thioredoxin (TXN) and thioredoxin-interacting protein (TXNIP) expression as biomarkers for predicting gastric cancer recurrence. METHODS: TXN and TXNIP expression levels were acquired from gene expression microarray data for 65 human gastric cancer tissues. We determined whether each gene expression level was associated with cancer recurrence and investigated the relationship between the two genes. For validation, the expression levels of TXN and TXNIP were measured by quantitative real-time reverse transcription polymerase chain reaction in 68 independent stage III gastric cancer patients. The correlation between gene expression and cancer prognosis was evaluated. Immunohistochemical staining was performed to investigate the protein expression levels of TXN and TXNIP and to characterize the expression patterns of each protein. RESULTS: TXN was a prognosis-related gene (P = 0.009), whereas TXNIP, a TXN inhibitor, demonstrated a negative correlation with TXN in the gene expression microarray data. In the 68 stage III patients, the expression levels of both TXN and TXNIP had a statistically significant effect on recurrence-free survival (RFS, P = 0.008 and P = 0.036, respectively). The low TXN and high TXNIP expression group exhibited a better prognosis than the other groups, and the high TXN and low TXNIP expression group exhibited a poorer prognosis (P < 0.001 for RFS and P = 0.001 for overall survival). More than half of the patients in the simultaneously high TXN and low TXNIP expression group experienced a recurrence within 1 year after curative surgery, and the 5-year survival rate of the patients in this group was 29%, compared with 89% in the low TXN and high TXNIP expression group. The TXN protein was overexpressed in 65% of the gastric cancer tissues, whereas the TXNIP protein was underexpressed in 85% of the cancer cells. In a correlation analysis, TXN and TXNIP were highly correlated with many oncogenes and tumor suppressors as well as with genes related to energy, protein synthesis and autophagy. CONCLUSION: TXN and TXNIP are promising prognostic markers for gastric cancer, and performing personalized adjuvant treatment based on TXN and TXNIP expression levels would be an effective practice in the treatment of gastric cancer.
Analgesic Effect of Harpagophytum procumbens on Postoperative and Neuropathic Pain in Rats
Dong Wook Lim,Jae Goo Kim,Daeseok Han,Yun Tai Kim
Molecules , 2014, DOI: 10.3390/molecules19011060
Abstract: Harpagophytum procumbens, also known as Devil’s Claw, has historically been used to treat a wide range of conditions, including pain and arthritis. The study was designed to investigate whether H. procumbens extracts exhibit analgesic effects in plantar incision and spared nerve injury (SNI) rats. The whole procedure was performed on male SD rats. To evaluate pain-related behavior, we performed the mechanical withdrawal threshold (MWT) test measured by von Frey filaments. Pain-related behavior was also determined through analysis of ultrasonic vocalization (USVs). The results of experiments showed MWT values of the group that was treated with 300 mg/kg H. procumbens extract increased significantly; on the contrary, the number of 22–27 kHz USVs of the treated group was reduced at 6 h and 24 h after plantar incision operation. After 21 days of continuous treatment with H . procumbens extracts at 300 mg/kg, the treated group showed significantly alleviated SNI-induced hypersensitivity responses by MWT, compared with the control group. These results suggest that H. procumbens extracts have potential analgesic effects in the case of acute postoperative pain and chronic neuropathic pain in rats.
Preventive Effects of Eleutherococcus senticosus Bark Extract in OVX-Induced Osteoporosis in Rats
Dong Wook Lim,Jae Goo Kim,Youngseok Lee,Seok Ho Cha,Yun Tai Kim
Molecules , 2013, DOI: 10.3390/molecules18077998
Abstract: Eleutherococcus senticosus (Siberian ginseng), has been used as a powerful tonic herb with an impressive range of health benefits. This medicinal herb has been commonly used to treat bone metabolism diseases due to its traditional Korean medicine use to strengthen muscle and bone. This study was conducted to investigate prevention of bone loss by a standardized extract of dried E. senticosus stem bark in an ovariectomized (OVX) rat model of osteoporosis. The OVX groups were divided into five groups treated with distilled water, 17β-estradiol (E2 10 μg/kg, once daily, i.p) and dried stem bark of E. senticosus extracts (DES 10, 30, and 100 mg/kg, once daily, p.o) for eight weeks, respectively. After eight weeks of treatments, the femur bone mineral density of the 100 mg/kg DES-treated group was significantly higher than that of the OVX-control group (16.7%, p < 0.01) without affecting the body, organs, and uterus weights, and serum estradiol levels. Additionally, bone markers such as serum ALP, CTx, and OC levels were significantly decreased in the DES 100 mg/kg treated group. These results show that DES is able to prevent OVX-induced in bone loss without the influence of hormones such as estrogen.
Oncothermia with Chemotherapy in the Patients with Small-Cell Lung Cancer
Doo Yun Lee,Seok Jin Haam,Tae Hoon Kim,Jae Yun Lim,Eun Jung Kim,Na Young Kim
Conference Papers in Science , 2013, DOI: 10.1155/2013/910363
Abstract: Small-cell lung cancer (SCLC) is one of the most aggressive and lethal forms of lung cancers. Chemotherapy and radiotherapy would be standard modality for SCLC with median survival being less than 4 months only. Complementary treatment to chemotherapy is desired. Oncothermia will be one of the candidates to this addition. We have made a study of 31 SCLC patients from April 2006 to March 2012. 23 cases were treated with combined chemotherapy and oncothermia, and 8 cases were treated with chemotherapy alone. Three patients from 14 patients (14/31) died in the study period; there were equal numbers in the two arms, including one long survival case of 28 months and one of 26 months, in the combination and chemo-group, respectively .16 patients (16/31) are alive: 4 patients with chemotherapy only, including one long survival case of 28.7 months, and 11 cases with combined therapy including three long survival cases of more than 3 years. We conclude that the combined use of chemotherapy and oncothermia has significantly enhanced the survival rate in comparison with the use of chemotherapy alone (log-rank test: P value < 0.02). 1. Introduction and Background Lung cancer is one of the most common causes of cancer-related deaths in both men and women worldwide. Its incidence as well as mortality rates is high, and the prognosis is usually very poor [1]. Its age-standardized incidence and mortality rates in 2006 were estimated to be 75.3 and 64.8/100?000/year, respectively, in men and 18.3 and 15.1/100?000/year in women in Europe, where the small-cell lung cancer (SCLC) accounts for 15%–18% of all cases [2]. The small-cell lung cancer has a fast growth-rate, disseminated quickly around the mediastinal lymph nodes, and forms distant metastases in late diagnosis, and then the median survival is only 2–4 months; the overall prognosis is very poor [3, 4]. Surgical treatment is not possible in almost all SCLC cases; it could be performed in very limited stage of the disease (i.e. T1,N0) only [2]; consequently the main treatments are chemo- and radiotherapy. The overall 2-year survival rate is less than 20%; and 5-year survival rate is almost devoid. In 50% of relapse SCLC cases chemotherapy reached complete remission (CR). In these the bulky primary tumors were completely destroyed but most intrathoracic recurrence was difficult to discover. In cases when radiotherapy was added, [5], recurrence rate has been reduced in 30–60% of the cases, however radiation pneumonitis, esophagitis was observed, but the overall survival rate was significantly improved [6]. Moreover,
Gene Expression Signature Analysis Identifies Vorinostat as a Candidate Therapy for Gastric Cancer
Sofie Claerhout, Jae Yun Lim, Woonyoung Choi, Yun-Yong Park, KyoungHyun Kim, Sang-Bae Kim, Ju-Seog Lee, Gordon B. Mills, Jae Yong Cho
PLOS ONE , 2011, DOI: 10.1371/journal.pone.0024662
Abstract: Background Gastric cancer continues to be one of the deadliest cancers in the world and therefore identification of new drugs targeting this type of cancer is thus of significant importance. The purpose of this study was to identify and validate a therapeutic agent which might improve the outcomes for gastric cancer patients in the future. Methodology/Principal Findings Using microarray technology, we generated a gene expression profile of human gastric cancer–specific genes from human gastric cancer tissue samples. We used this profile in the Broad Institute's Connectivity Map analysis to identify candidate therapeutic compounds for gastric cancer. We found the histone deacetylase inhibitor vorinostat as the lead compound and thus a potential therapeutic drug for gastric cancer. Vorinostat induced both apoptosis and autophagy in gastric cancer cell lines. Pharmacological and genetic inhibition of autophagy however, increased the therapeutic efficacy of vorinostat, indicating that a combination of vorinostat with autophagy inhibitors may therapeutically be more beneficial. Moreover, gene expression analysis of gastric cancer identified a collection of genes (ITGB5, TYMS, MYB, APOC1, CBX5, PLA2G2A, and KIF20A) whose expression was elevated in gastric tumor tissue and downregulated more than 2-fold by vorinostat treatment in gastric cancer cell lines. In contrast, SCGB2A1, TCN1, CFD, APLP1, and NQO1 manifested a reversed pattern. Conclusions/Significance We showed that analysis of gene expression signature may represent an emerging approach to discover therapeutic agents for gastric cancer, such as vorinostat. The observation of altered gene expression after vorinostat treatment may provide the clue to identify the molecular mechanism of vorinostat and those patients likely to benefit from vorinostat treatment.
Midkine, heparin-binding growth factor, blocks kainic acid-induced seizure and neuronal cell death in mouse hippocampus
Yun B Kim, Jae K Ryu, Hong J Lee, In J Lim, Dongsun Park, Min C Lee, Seung U Kim
BMC Neuroscience , 2010, DOI: 10.1186/1471-2202-11-42
Abstract: Increased expression of MK was found in hippocampus of mouse following seizures induced by intracerebroventricular injection of KA, and MK expression was found in glial fibrillary acidic protein (GFAP)-positive astrocytes. Concurrent injection of MK and KA attenuated KA-induced seizure activity and cell death of hippocampal neurons including pyramidal cells and glutamic acid decarboxylase 67 (GAD67)-positive GABAergic interneurons in the CA3 and hilar area.The results of the present study indicate that MK functions as an anticonvulsant and neuroprotective agent in hippocampus during KA-induced seizures.Temporal lobe epilepsy (TLE) is pathologically characterized by extensive neuronal loss in the CA1, CA3 and hilar regions of hippocampus [1,2]. Previous studies have demonstrated that the animal models of TLE generated by intracerebroventricular injection of kainic acid (KA) faithfully reproduce clinical and pathological features found in human TLE [3-7].Previous studies have reported the possible involvement of neurotrophic factors in epilepsy as suggested by the gene expression of neurotrophic factors such as NGF, BDNF and NT-3 in hippocampus in human TLE as well as in TLE animal models [8,9]. Midkine (MK), one of such neurotrophic factors, has emerged as an important neuromodulator in the central nervous system (CNS). MK, a member of the heparin-binding growth factor family, which includes MK and pleiotrophin, is known to possess neurotrophic and neuroprotective properties [10,11]. MK was originally isolated as the product of retinoic acid-responsive gene that functions primarily in inducing cell differentiation in mouse teratocarcinoma cells [12], and has the ability to influence a variety of neuronal functions including neurite extension [13], neuronal differentiation [14,15] and neuronal survival following injury or damage in the CNS [15,16]. During the fetal development of the CNS, MK expression was demonstrated in neuroepithelial/neural progenitor cells follow
Regulation of Wound Healing by Granulocyte-Macrophage Colony-Stimulating Factor after Vocal Fold Injury
Jae-Yol Lim, Byung Hyune Choi, Songyi Lee, Yun Ho Jang, Jeong-Seok Choi, Young-Mo Kim
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0054256
Abstract: Objectives Vocal fold (VF) scarring remains a therapeutic challenge. Granulocyte-macrophage colony-stimulating factor (GM-CSF) facilitates epithelial wound healing, and recently, growth factor therapy has been applied to promote tissue repair. This study was undertaken to investigate the effect of GM-CSF on VF wound healing in vivo and in vitro. Methods VF scarring was induced in New Zealand white rabbits by direct injury. Immediately thereafter, either GM-CSF or PBS was injected into the VFs of rabbits. Endoscopic, histopathological, immunohistochemical, and biomechanical evaluations of VFs were performed at 3 months post-injury. Human vocal fold fibroblasts (hVFFs) were cultured with GM-CSF. Production of type I and III collagen was examined immunocytochemically, and the synthesis of elastin and hyaluronic acids was evaluated by ELISA. The mRNA levels of genes related to ECM components and ECM production-related growth factors, such as HGF and TGF-?1, were examined by real time RT-PCR. Results The GM-CSF-treated VFs showed reduced collagen deposition in comparison to the PBS-injected controls (P<0.05). Immunohistochemical staining revealed lower amounts of type I collagen and fibronectin in the GM-CSF-treated VFs (P<0.05 and P<0.01, respectively). Viscous and elastic shear moduli of VF samples were significantly lower in the GM-CSF group than in the PBS-injected group (P<0.001 and P<0.01, respectively). Mucosal waves in the GM-CSF group showed significant improvement when compared to the PBS group (P = 0.0446). GM-CSF inhibited TGF-β1-induced collagen synthesis by hVFFs (P<0.05) and the production of hyaluronic acids increased at 72 hours post-treatment (P<0.05). The expressions of HAS-2, tropoelastin, MMP-1, HGF, and c-Met mRNA were significantly increased by GM-CSF, although at different time points (P<0.05). Conclusion The present study shows that GM-CSF offers therapeutic potential for the remodeling of VF wounds and the promotion of VF regeneration.
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