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Search Results: 1 - 10 of 9794 matches for " JS.;Marzocchi-Machado "
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Leukocytes respiratory burst and lysozyme level in pacu (Piaractus mesopotamicus Holmberg, 1887)
Abreu, JS.;Marzocchi-Machado, CM.;Urbaczek, AC.;Fonseca, LM.;Urbinati, EC.;
Brazilian Journal of Biology , 2009, DOI: 10.1590/S1519-69842009000500018
Abstract: innate immune responses are useful to determine the health status of fish and to evaluate the effect of immunomodulatory substances in fish farming. leukocytes respiratory burst was measured in pacu (piaractus mesopotamicus) using chemiluminescence assay and nitroblue tetrazolium (nbt) reduction assay. the nitroblue tetrazolium reduction seemed more adequate than chemiluminescence assay for leukocytes oxidative burst determination, since it was difficult to isolate the blood leucocytes for chemiluminescence assay. plasma and serum lysozyme were measured using a turbidimetric assay. the heating of serum and plasma samples (56 oc for 30 minutes) for complement system inactivation darkened the plasma samples and interfered in the results. the lysozyme activity in serum was higher than in plasma, suggesting that serum samples are more appropriate for the analysis. this study established protocols that can be useful tools in the study of immune mechanisms of the tropical fish pacu.
Hemolytic activity of alternative complement pathway as an indicator of innate immunity in pacu (Piaractus mesopotamicus)
Biller-Takahashi, Jaqueline Dalbello;Takahashi, Leonardo Susumu;Marzocchi-Machado, Cleni Mara;Zanuzzo, Fabio Sabbadin;Sabioni, Rafael Estevan;Urbinati, Elisabeth Criscuolo;
Revista Brasileira de Zootecnia , 2012, DOI: 10.1590/S1516-35982012000200001
Abstract: the objective of this study was to evaluate the methodology to establish the hemolytic activity of alternative complement pathway as an indicator of the innate immunity in brazilian fish pacu (piaractus mesopotamicus), in addition to verifying the influence of β-glucan as an immunostimulant. fish were fed with diets containing 0, 0.1 and 1% β-glucan, during seven days, and then inoculated with aeromonas hydrophila. seven days after the challenge, they were bled for serum extraction. the methodology consisted of a kinetic assay that allows calculating the required time for serum proteins of the complement to promote 50% lysis of a rabbit red blood cell suspension. the method developed in mammals was successfully applied for pacu and determined that the hemolytic activity of the proteins of the complement system (alternative pathway) increased after the pathogen challenge, but was not influenced by the β-glucan treatment.
Earthquake forecasting in Italy, before and after Umbria-Marche seismic sequence 1997. A review of the earthquake occurrence modeling at different spatio-temporal-magnitude scales.
W. Marzocchi
Annals of Geophysics , 2008, DOI: 10.4401/ag-4451
Abstract: The main goal of this work is to review the scientific researches carried out before and after the Umbria-Marche sequence related to the earthquake forecasting/prediction in Italy. In particular, I focus the attention on models that aim addressing three main practical questions: was (is) Umbria-Marche a region with high probability of occurrence of a destructive earthquake? Was a precursory activity recorded before the mainshock(s)? What was our capability to model the spatio-temporal-magnitude evolution of that seismic sequence? The models are reviewed pointing out what we have learned after the Umbria-Marche earthquakes, in terms of physical understanding of earthquake occurrence process, and of improving our capability to forecast earthquakes and to track in real-time seismic sequences.
The Proportional Hazard Model as applied to the CSEP forcasting area in Italy
Licia Faenza,Warner Marzocchi
Annals of Geophysics , 2010, DOI: 10.4401/ag-4759
Abstract: This study presents the strategies adopted to modify the Proportional Hazard Model to fit the requirements for forecasting testing within the Collaboratory Study for Earthquake Predictability (CSEP) experiment. The model was originally proposed to study the spatiotemporal distribution of M5.5+ seismicity in Italy, through two spatial models: a regular grid, and a seismotectonic zonation. A prospective 10-year-forecast test has already been ongoing since 2005, and the results are available on the internet (http://www.bo.ingv.it/earthquake/ ITALY/forecasting/M5.5+/). For that test, we have reported the probability maps of M5.5+ earthquakes for the next 10 years for the two spatial models. As the original model is time-dependent, it is updated every year, and also immediately after the occurrence of a target event, e.g., Mw5.5. Although this prospective test is continuing and the model updates probabilities that are different from those of the CSEP experiments, we argue that a full evaluation of the model can only be achieved through this CSEP testing, where the performances of different models are compared using the same rules and tests. The major modification we have introduced into our model is the simulation of the expected numbers of events in the exposure time Dx. This is performed considering the probability that an event occurs in Dx, and evaluating the change this will cause in the expected number of events. This procedure is also implemented for the first and second generation of aftershocks.
A technical note on the bias in the estimation of the b-value and its uncertainty through the Least Squares technique
L. Sandri,W. Marzocchi
Annals of Geophysics , 2007, DOI: 10.4401/ag-4422
Abstract: We investigate conceptually, analytically, and numerically the biases in the estimation of the b-value of the Gutenberg-Richter Law and of its uncertainty made through the least squares technique. The biases are introduced by the cumulation operation for the cumulative form of the Gutenberg-Richter Law, by the logarithmic transformation, and by the measurement errors on the magnitude. We find that the least squares technique, applied to the cumulative and binned form of the Gutenberg-Richter Law, produces strong bias in the b-value and its uncertainty, whose amplitudes depend on the size of the sample. Furthermore, the logarithmic transformation produces two different endemic bends in the Log(N) versus M curve. This means that this plot might produce fake significant departures from the Gutenberg-Richter Law. The effect of the measurement errors is negligible compared to those of cumulation operation and logarithmic transformation. The results obtained show that the least squares technique should never be used to determine the slope of the Gutenberg-Richter Law and its uncertainty.
A review and new insights on the estimation of the b-valueand its uncertainty
W. Marzocchi,L. Sandri
Annals of Geophysics , 2003, DOI: 10.4401/ag-3472
Abstract: The estimation of the b-value of the Gutenberg-Richter Law and its uncertainty is crucial in seismic hazard studies, as well as in verifying theoretical assertions, such as, for example, the universality of the Gutenberg-Richter Law. In spite of the importance of this issue, many scientific papers still adopt formulas that lead to different estimations. The aim of this paper is to review the main concepts relative to the estimation of the b-value and its uncertainty, and to provide some new analytical and numerical insights on the biases introduced by the unavoidable use of binned magnitudes, and by the measurement errors on the magnitude. It is remarked that, although corrections for binned magnitudes were suggested in the past, they are still very often neglected in the estimation of the b-value, implicitly by assuming that the magnitude is a continuous random variable. In particular, we show that: i) the assumption of continuous magnitude can lead to strong bias in the b-value estimation, and to a significant underestimation of its uncertainty, also for binning of ?M = 0.1; ii) a simple correction applied to the continuous formula causes a drastic reduction of both biases; iii) very simple formulas, until now mostly ignored, provide estimations without significant biases; iv) the effect on the bias due to the measurement errors is negligible compared to the use of binned magnitudes.
A technical note on the bias in the estimation of the b-value and its uncertainty through the Least Squares technique
L. Sandri,W. Marzocchi
Annals of Geophysics , 2007, DOI: 10.4401/ag-4432
Abstract: We investigate conceptually, analytically, and numerically the biases in the estimation of the b-value of the Gutenberg-Richter law and of its uncertainty made through the least squares technique. The biases are introduced by the cumulation operation for the cumulative form of the Gutenberg-Richter law, by the logarithmic transformation, and by the measurement errors on the magnitude. We find that the least squares technique, applied to the cumulative and binned form of the Gutenberg-Richter law, produces strong bias in the b-value and its uncertainty, whose amplitudes depend on the size of the sample. Furthermore, the logarithmic transformation produces two different endemic bends in the Log(N) versus M curve. This means that this plot might produce fake significant departures from the Gutenberg-Richter law. The effect of the measurement errors is negligible compared to those of cumulation operation and logarithmic transformation. The results obtained show that the least squares technique should never be used to determine the slope of the Gutenberg-Richter law and its uncertainty.
Surface energy arising from the behavior of lipid molecules in the water via Γ-convergence
Luca Lussardi,Alfredo Marzocchi
Atti della Accademia Peloritana dei Pericolanti : Classe di Scienze Fisiche, Matematiche e Naturali , 2013, DOI: 10.1478/aapp.91s1a12
Abstract: We show, in the framework of Γ-convergence, that a surface energy of area type arises from a probabilistic model for lipid molecules in water.
On the dependence on p of the variational eigenvalues of the p-Laplace operator
Marco Degiovanni,Marco Marzocchi
Mathematics , 2015,
Abstract: We study the behavior of the variational eigenvalues of the p-Laplace operator, with homogeneous Dirichlet boundary condition, when p is varying. After introducing an auxiliary problem, we characterize the continuity answering, in particular, a question raised in [Lindqvist, 1993].
A double-branching model applied to long-term forecasting of Italian seismicity (ML≥5.0) within the CSEP project
Anna Maria Lombardi,Warner Marzocchi
Annals of Geophysics , 2010, DOI: 10.4401/ag-4762
Abstract: The purpose of this study was to apply a double-branching model to forecasting of moderate-to-large Italian seismicity within the Collaboratory Study for Earthquake Predictability project. This project is designed for statistical evaluations and comparisons of various forecasting models, on both the global and regional scales. This proposed double-branching model is time-dependent, as it assumes that each earthquake can generate, or is correlated with, other earthquakes through physical mechanisms that act on different spatio-temporal scales. Specifically, it consists of an application of two branching processes, in which any earthquake can trigger a family of later events on different space-time scales. In our recent study [Lombardi and Marzocchi 2009], we applied this model to a declustered historical database that included the strong Italian seismicity from over the last few centuries. This catalog only allowed us to describe the long-term time evolution of moderate-to-strong seismicity. Here, we have applied this double-branching model to a new database that has allowed us to describe both short-term clustering and long-term features at the same time. As the model can produce forecasting calculations of future seismicity, we provide some probability maps of occurrence of predicted events over different temporal windows.
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