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Search Results: 1 - 10 of 11 matches for " JLV;Bunger "
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Identification of turkey astrovirus and turkey coronavirus in an outbreak of Poult Enteritis and Mortality Syndrome
Villarreal, LYB;Assayag, MS;Brand?o, PE;Chacón, JLV;Bunger, AND;Astolfi-Ferreira, CS;Gomes, CR;Jones, RC;Ferreira, AJP;
Revista Brasileira de Ciência Avícola , 2006, DOI: 10.1590/S1516-635X2006000200010
Abstract: this article reports a survey on turkey astrovirus (tastv) and turkey coronavirus (tcov) infections with rt-pcr in 17 turkey flocks affected by acute enteritis and two apparently normal turkey flocks located in the southeastern region of brazil by pcr (tastv and tcov). seven out of the 17 affected flocks were positive for tastv and 14 for tcov, with seven co-infections. in one of the two apparently normal flocks, a tastv-tcov co-infection was found. although a definitive association of these agents and the signs can not be made, the implications of these findings are discussed.
Rhabdomyolysis secondary to an accident with marine stingray (Dasyatis family)
Masson, AA;Ormonde do Carmo, PHA;Carvalho, JLV;
Journal of Venomous Animals and Toxins including Tropical Diseases , 2012, DOI: 10.1590/S1678-91992012000300013
Abstract: whenever humans come in touch with the sea, they become vulnerable to risks, most frequently on account of invading a habitat that belongs to potentially harmful aquatic animals. world literature shows a growing number of incidents in recent years with marine stingrays, despite the fact that these animals only attack when harassed. this report describes an accident in which an amateur fisherman was injured by a marine stingray, probably of the dasyatis family, on his left forearm. the puncture wound was highly painful and progressed to rhabdomyolysis. it is conjectured that lymphatic drainage, applied in an attempt to reduce edema and its complications, may have contributed to rebound increase in serum creatine kinase levels (ck) and, thus, has exacerbated the intensity of rhabdomyolysis. therefore, as a measure of caution, lymphatic drainage should not enter current treatment protocols for similar cases, until new studies are performed in order to clarify this issue.
Survey of infectious laryngotracheitis outbreak in layer hens and differential diagnosis with other respiratory pathogens
Chacón, JLV;Brand?o, PEB;Villarreal, LYB;Gama, NM;Ferreira, AJP;
Revista Brasileira de Ciência Avícola , 2007, DOI: 10.1590/S1516-635X2007000100009
Abstract: trachea, lung, and conjunctive samples from 51 commercial layer farms from bastos region, s?o paulo, brazil, were submitted to nested-pcr and virus isolation in spf chicken embryos for ilt diagnosis. this region experienced an outbreak characterized by respiratory signs, decrease in egg production and increased mortality. out of the 51 tested field samples, 23 were positive for ilt by nested-pcr, 22 were positive after the virus isolation, and 24 were positive when both techniques were used. newcastle disease virus, avian pneumovirus, or mycoplasma gallisepticum were not detected. infectious bronchitis virus was detected in one farm and mycoplasma synoviae was detected in eight farms. the high incidence of infectious laryngotracheitis virus (iltv) detection, the high correlation between the observed clinical signs and the iltv detection, and the results of differential diagnosis demonstrated that iltv was the causative agent of the outbreak of respiratory disease observed in bastos region, s?o paulo, brazil.
Caracterización química y sensorial de biscochuelos enriquecidos con fibra dietética y micronutrientes para el anciano
Wittig de Penna,Emma; Avenda?o,Paula; Soto,Delia; Bunger,Andrea;
Archivos Latinoamericanos de Nutrición , 2003,
Abstract: summary. this study presents the development of individual cakes enriched with dietary fiber (lupin and oat fiber), vitamins and minerals; as recent research has shown that the average daily dietary fibre intake of the elderly population in chile is only 12 g. each cake contains 4.8 g of dietary fibre and a 30% of the rda of vitamins a, b1, b2, b6, b12, e, nicotinamide and folic acid, 40% of the rda of vitamin d3, 15% of the rda of calcium, 12% of the rda of magnesium and 3% the rda of zinc. polydextrose and sorbitol were added to improve flavor and texture. response surface methodology (rsm) was used for optimization, based on a two-variable composite design. thirteen experimental runs were carried out, with polydextrose (1 to 40% based on flour content) and sorbitol (1 to 30% based on flour content) as independent variables. the response variable was sensory quality obtained by the karlsruhe test. sensory attributes of texture and overall quality showed a good fitting with high determination coefficients and were used for optimization. the optimized cake contained 11.5% polydextrose and 4.4% sorbitol (both based on flour content). quality of the optimized product was controlled by means of physical, chemical, microbiological and sensory analyses. overall sensory quality was 8,18 ("very good") and good quality and nutritive value were achieved. in an acceptance test carried out with 150 adults, 100% acceptability was obtained in the hedonic scale categories "like it" and "like it very much". a shelf life study performed with cakes packaged in polypropylene bags of 30 m m thickness indicated a shelf life of 13 days stored at room conditions (25oc and 55-60%r.h.).
Entrenamiento de paneles sensoriales constituidos por ni?os
Wittig de Penna,Emma; Bunger Timemlann,Andrea; Serrano Valdés,Lissis;
Archivos Latinoamericanos de Nutrición , 2000,
Abstract: sensory training of children. in the development of food products for children, it is advisable to establish the characteristics of the product with groups of children that represent the target population. to ensure the success of the products, the quality and hedonic satisfaction expectatives must be considered. in order to accomplish this premises, a group of children under the program complementary feeding of the health ministry- was selected and trained. the project was developed with a group of 33 children ages 9 to 12 years- from the republica of colombia school of santiago, whose parents agreed and supported the participation of their children in this project. the first step was teaching the technics and methodology of sensory evaluation, and increasing their sensitivity. after the 8 programmed sessions, those children who met the minimal requiremients for a training group were chosen. the second step was performed during 12 sessions, working with 14 children. the training was aimed at the development of the vocabulary to describe quality and detects, ranking tests, discriminative tests and the use of different scales. tests to verify reliability, veracity and reproducibility of judgements (p<0.05) were carried out. the trained group was able to assess different meals of the program. the obtained results allowed to propase the improvement of some quality criteria of the program meals.
Shiga Toxin Type 2dact Displays Increased Binding to Globotriaosylceramide in vitro and Increased Lethality in Mice after Activation by Elastase
Joshua C. Bunger,Angela R. Melton-Celsa,Alison D. O'Brien
Toxins , 2013, DOI: 10.3390/toxins5112074
Abstract: Shiga toxin type 2dact (Stx2dact), an Stx2 variant originally identified from Escherichia coli O91:H21 strain B2F1, displays increased cytotoxicity after activation by elastase present in intestinal mucus. Activation is a result of cleavage of two amino acids from the C-terminal tail of the A 2 subunit. In this study, we hypothesized that activation leads to increased binding of toxin to its receptor on host cells both in vitro and in vivo. To test this theory, Stx2dact was treated with elastase or buffer alone and then each toxin was assessed for binding to purified globotriaosylceramide (Gb3) in an enzyme-linked immunosorbent assay, or cells in culture by immunofluorescence, or flow cytometry. Elastase- and buffer-treated Stx2dact were also evaluated for binding to mouse kidney tissue and for relative lethality in mice. We found that activated Stx2dact had a greater capacity to bind purified Gb3, cells in culture, and mouse kidney tissue and was more toxic for mice than was non-activated Stx2dact. Thus, one possible mechanism for the augmented cytotoxicity of Stx2dact after activation is its increased capacity to bind target cells, which, in turn, may cause greater lethality of elastase-treated toxin for mice and enhanced virulence for humans of E. coli strains that express Stx2dact.
Rotavirus detection and isolation from chickens with or without symptoms
Villarreal, LYB;Uliana, G;Valenzuela, C;Chacón, JLV;Saidenberg, ABS;Sanches, AA;Brand?o, PE;Jerez, JA;Ferreira, AJP;
Revista Brasileira de Ciência Avícola , 2006, DOI: 10.1590/S1516-635X2006000300009
Abstract: rotaviruses have been identified as one of the main etiological agents of diarrhea and enteritis in mammals, including humans, and in avian species. few studies have been published about enteric viruses in brazilian poultry, including those related to rotavirus infection. such studies demonstrate significant occurrence and the importance of enteric viruses in poultry presenting intestinal problems. enteric viruses are the primary cause of injuries to the gut, allowing other agents, especially bacteria, to attach, to penetrate, and to replicate in the enteric tissue, leading to further damage. the aim of the present study was to detect rotavirus in the intestinal contents of layers and broilers by polyacrylamide gel electrophoresis (page) and virus isolation in ma-104 cell culture. a total of 45.3% of all samples were positive to rotavirus; rotavirus frequencies were 48.7% in samples from flocks with diarrhea, 46.4% in flocks with delayed growth, and 30% in asymptomatic flocks. it was possible to isolate rotavirus in ma-104 cells from the nine rotavirus-positive randomly chosen samples. these results indicate that rotavirus may have an important role in pathogenesis of enteric disease.
Optimización de una formulacion de espaguetis enriquecidos con fibra dietética y micronutrientes para el adulto mayor
Wittig de Penna,Emma; Serrano,Lisis; Bunger,Andrea; Soto,Delia; López,Luis; Hernández,Nieves; Ruales,Jenny;
Archivos Latinoamericanos de Nutrición , 2002,
Abstract: optimization of a spaghetti formula enriched wich dietary fibre and micronutrients for elderly people. several studies have demonstrated low dietary fiber intake in elderly people, which increases the risk of diseases such as constipation, colon cancer and diverticulosis. a spaghetti formula enriched with lupin fibre was developed to increase the dietary fibre intake in elderly people, as spaghetti are frequently consumed in this age group. sweet lupin bran (vitafiberr) was used as fibre source and gluten was used as improving additive. response surface methodology with a two variable composite rotatable design was applied to optimize the formulations. the independent variables were lupin bran (7.14 -14.29%) and gluten vital (0.1-2.0%). the dependent variables were the responses of a trained 10-member sensory panel who evaluated the sensory quality parameters color, shape, aroma, flavor and texture by the karlsruhe 9-point test. the optimized formula was prepared with 66.7% semoline, 7.14% lupin bran, 1.05% gluten and 24.7% water, enriched with 0.019% of a vitamin premix (a. e, d, b2, b12 and folic acid) and with 0.41% of a mineral premix (ca, fe, zn), in order to meet 30 % of the rda for the elderly per 100 g dry spaghetti. the dietary fibre content of the optimized product was 11.05 g/100g. the study showed that fibre-enriched spaghetti formula is a good way to increase dietary fibre intake in elderly people, as it is a common food, simple to prepare and easy to eat
Detection and molecular characterization of infectious laryngotracheitis virus in laying hens in Brazil
Villarreal, LYB;Brand?o, PEB;Chacón, JLV;Doretto Junior, L;Ito, N;Gama, NS;Ishizuka, MM;Luchese, A;Buchala, F;Astolfi-Ferreira, CS;Ferreira, AJP;
Revista Brasileira de Ciência Avícola , 2004, DOI: 10.1590/S1516-635X2004000400011
Abstract: avian infectious laryngotracheitis, caused by infectious laryngotracheitis virus (iltv), has been reported for decades in brazilian laying and broiler flocks. more recently, outbreaks have occurred in s?o paulo state. this study reports the application of pcr and dna sequencing targeted to the p32 gene of iltv using laying chicken samples from bastos, s?o paulo, brazil. three out of four field samples were positive by pcr. dna sequencing of two samples evidenced homology of the amplified fragments with the p32 gene of iltv. the results definitely confirmed the presence of iltv in the birds during the outbreak. further studies are needed to establish the sources of infection and to determine whether the detected virus was originated from vaccine or field virus strains.
Sex Pheromone Receptor Specificity in the European Corn Borer Moth, Ostrinia nubilalis
Kevin W. Wanner,Andrew S. Nichols,Jean E. Allen,Peggy L. Bunger,Stephen F. Garczynski,Charles E. Linn Jr.,Hugh M. Robertson,Charles W. Luetje
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0008685
Abstract: The European corn borer (ECB), Ostrinia nubilalis (Hubner), exists as two separate sex pheromone races. ECB(Z) females produce a 97:3 blend of Z11- and E11-tetradecenyl acetate whereas ECB(E) females produce an opposite 1:99 ratio of the Z and E isomers. Males of each race respond specifically to their conspecific female's blend. A closely related species, the Asian corn borer (ACB), O. furnacalis, uses a 3:2 blend of Z12- and E12-tetradecenyl acetate, and is believed to have evolved from an ECB-like ancestor. To further knowledge of the molecular mechanisms of pheromone detection and its evolution among closely related species we identified and characterized sex pheromone receptors from ECB(Z).
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