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Search Results: 1 - 10 of 593403 matches for " J. M. Granjeiro "
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Cytotoxicity Tests for Evaluating Medical Devices: An Alert for the Development of Biotechnology Health Products  [PDF]
M. N. P. Vidal, J. M. Granjeiro
Journal of Biomedical Science and Engineering (JBiSE) , 2017, DOI: 10.4236/jbise.2017.109033
Abstract: The risks and damages related to the use of products, technologies and services of sanitary interest can be due to defects or manufacturing failures. Certain products already contain a certain degree of risk, which requires strict quality control in their production, distribution and use, as well as in the disposal of their waste in the environment. With continuous development in science and technology, medical devices must undergo intradermal irritation and testing for sensitization, cytotoxicity, and acute systemic toxicity. In health care, biotechnology aims to provide technology-based products or processes related to energy, food, and health, which are capable of stimulating new businesses, expanding exports, integrating the value chain and stimulating new demands for innovative products and processes, taking into account health policies. The present article was prepared by a bibliographical survey of the electronic databases PubMed, Lilacs, and Bireme. Cell culture testing can be successfully employed, as it is reproducible, rapid, sensitive, and financially accessible for performing in vitro toxicity testing. Thus, it has been possible to optimize the development phase of new products by decreasing animal use or even replacing them in certain tests. Some in vitro assays validated by the Organization for Economic Cooperation and Development in the area of health products have already replaced animal testing.
Bone morphogenetic proteins: from structure to clinical use
Granjeiro, J.M.;Oliveira, R.C.;Bustos-Valenzuela, J.C.;Sogayar, M.C.;Taga, R.;
Brazilian Journal of Medical and Biological Research , 2005, DOI: 10.1590/S0100-879X2005001000003
Abstract: bone morphogenetic proteins (bmps) are multi-functional growth factors belonging to the transforming growth factor ? superfamily. family members are expressed during limb development, endochondral ossification, early fracture, and cartilage repair. the activity of bmps was first identified in the 1960s but the proteins responsible for bone induction were unknown until the purification and cloning of human bmps in the 1980s. to date, about 15 bmp family members have been identified and characterized. the signal triggered by bmps is transduced through serine/threonine kinase receptors, type i and ii subtypes. three type i receptors have been shown to bind bmp ligands, namely: type ia and ib bmp receptors and type ia activin receptors. bmps seem to be involved in the regulation of cell proliferation, survival, differentiation and apoptosis, but their hallmark is their ability to induce bone, cartilage, ligament, and tendon formation at both heterotopic and orthotopic sites. this suggests that, in the future, they may play a major role in the treatment of bone diseases. several animal studies have illustrated the potential of bmps to enhance spinal fusion, repair critical-size defects, accelerate union, and heal articular cartilage lesions. difficulties in producing and purifying bmps from bone tissue have prompted the attempts made by several laboratories, including ours, to express these proteins in the recombinant form in heterologous systems. this review focuses on bmp structure, molecular mechanisms of action and significance and potential applications in medical, dental and veterinary practice for the treatment of cartilage and bone-related diseases.
Bone morphogenetic proteins: from structure to clinical use
Granjeiro J.M.,Oliveira R.C.,Bustos-Valenzuela J.C.,Sogayar M.C.
Brazilian Journal of Medical and Biological Research , 2005,
Abstract: Bone morphogenetic proteins (BMPs) are multi-functional growth factors belonging to the transforming growth factor superfamily. Family members are expressed during limb development, endochondral ossification, early fracture, and cartilage repair. The activity of BMPs was first identified in the 1960s but the proteins responsible for bone induction were unknown until the purification and cloning of human BMPs in the 1980s. To date, about 15 BMP family members have been identified and characterized. The signal triggered by BMPs is transduced through serine/threonine kinase receptors, type I and II subtypes. Three type I receptors have been shown to bind BMP ligands, namely: type IA and IB BMP receptors and type IA activin receptors. BMPs seem to be involved in the regulation of cell proliferation, survival, differentiation and apoptosis, but their hallmark is their ability to induce bone, cartilage, ligament, and tendon formation at both heterotopic and orthotopic sites. This suggests that, in the future, they may play a major role in the treatment of bone diseases. Several animal studies have illustrated the potential of BMPs to enhance spinal fusion, repair critical-size defects, accelerate union, and heal articular cartilage lesions. Difficulties in producing and purifying BMPs from bone tissue have prompted the attempts made by several laboratories, including ours, to express these proteins in the recombinant form in heterologous systems. This review focuses on BMP structure, molecular mechanisms of action and significance and potential applications in medical, dental and veterinary practice for the treatment of cartilage and bone-related diseases.
Recobrimento da liga Ti-6Al-4V com hidroxiapatita pelo método sol-gel e sua aplica??o a hastes femorais n?o-cimentadas
Avés, E. P.;Galván, J. C.;Lima, I. R.;Granjeiro, J. M.;Bastos, I. N.;Soares, G. D.;
Ceramica , 2008, DOI: 10.1590/S0366-69132008000400015
Abstract: the coating of metallic alloys with bioactive ceramics aims to accelerate bone formation around the implant, contributing to its fixation. in this paper, the deposition of hydroxyapatite ceramic on ti-6al-4v alloy sheets by the sol-gel method was studied. the coating layer was characterized by scanning electron microscopy, x-ray diffraction and its adhesion to substrate was evaluated by shear testing. the citocompatibility test shows that the sol-gel coating did not provoke the cell death significantly higher than the control (p > 0.05). moreover, femoral stems removed from patient (explants) were adequately coated using the sol-gel process.
Mistura de proteínas morfogenéticas ósseas, hidroxiapatita, osso inorganico e colágeno envolta por membrana de pericárdio no preenchimento de defeito ósseo segmentar em coelhos
Ciani, R.B.;Rahal, S.C.;Volpi, R.S.;Taga, R.;Granjeiro, J.M.;Cestari, T.M.;Mamprim, M.J.;
Arquivo Brasileiro de Medicina Veterinária e Zootecnia , 2006, DOI: 10.1590/S0102-09352006000100010
Abstract: biomaterials of bovine origin in regenerating segmental bone defects were evaluated. twelve six-month old norfolk rabbits, weighting 3 to 4.5kg were used. a 1cm long segmental defect was created in the radial diaphysis, including the periosteum, of both forelimbs. in the right forelimb, the defect was filled using a mixture of bone morphogenic proteins adsorbed to hydroxyapatite, agglutinant of lyophilized collagen in granules and anorganic cortical bone in granules delimited by a pericardial membrane. in the left forelimb, the defect did not receive treatment and served as a control. radiographies were taken immediately after surgery and at seven, 30, 60, 90, 120 and 150 days post-operatively. six rabbits were euthanized at 60 days and the other six at 150 days post-surgery for histological evaluation. radiographic and histological results revealed that bone regeneration was inhibited in the segmental defects receiving biomaterials.
Nanometer Scale Titanium Surface Texturing Are Detected by Signaling Pathways Involving Transient FAK and Src Activations
Willian F. Zambuzzi, Estevam A. Bonfante, Ryo Jimbo, Mariko Hayashi, Martin Andersson, Gutemberg Alves, Esther R. Takamori, Paulo J. Beltr?o, Paulo G. Coelho, José M. Granjeiro
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0095662
Abstract: Background It is known that physico/chemical alterations on biomaterial surfaces have the capability to modulate cellular behavior, affecting early tissue repair. Such surface modifications are aimed to improve early healing response and, clinically, offer the possibility to shorten the time from implant placement to functional loading. Since FAK and Src are intracellular proteins able to predict the quality of osteoblast adhesion, this study evaluated the osteoblast behavior in response to nanometer scale titanium surface texturing by monitoring FAK and Src phosphorylations. Methodology Four engineered titanium surfaces were used for the study: machined (M), dual acid-etched (DAA), resorbable media microblasted and acid-etched (MBAA), and acid-etch microblasted (AAMB). Surfaces were characterized by scanning electron microscopy, interferometry, atomic force microscopy, x-ray photoelectron spectroscopy and energy dispersive X-ray spectroscopy. Thereafter, those 4 samples were used to evaluate their cytotoxicity and interference on FAK and Src phosphorylations. Both Src and FAK were investigated by using specific antibody against specific phosphorylation sites. Principal Findings The results showed that both FAK and Src activations were differently modulated as a function of titanium surfaces physico/chemical configuration and protein adsorption. Conclusions It can be suggested that signaling pathways involving both FAK and Src could provide biomarkers to predict osteoblast adhesion onto different surfaces.
Langerhans Cell Histiocytosis in Otorhinolaryngology
Fernandes, Humberto de Barros,Granjeiro, Ronaldo Campos,Negreiros Júnior, Jacinto de
International Archives of Otorhinolaryngology , 2009,
Abstract: Introduction: The Langerhans cell histiocytosis is an inflammatory cells proliferative disorder of unknown etiology. It is uncommon disease in children. Objective: To proceed with a literature review on Langerhans cell histiocytosis, with focus on the otorhinolaryngological complications. Materials and methods: The methodology used was advised based on online data from MEDLINE, between 1966 and 2008, with research of terms related to Langerhans cell histiocytosis, temporal bones and otorhinolaryngology. Literature Review: The manifestations in the head and neck are the most common ones and their diagnosis becomes difficult once it mimetizing other more common diseases the otorhinolaryngologist sees as external ear eczema, acute mastoiditis and gingivitis. The temporal bone disease manifests as recurrent otorrhea and external auditory meatus and retroauricular granulomas. The radiological evaluation confirms lytic lesions especially in the cranial cap, jaw, temporal bones and spines. The definitive diagnosis is made by biopsy through the histopathological discoveries and immunohistochemistry detection of the CD1a antigen. The main form of treatment is by chemotherapy and, in a lower scale, radiotherapy or surgery. Conclusions: he otorhinolaryngological manifestations must be suspected for recurrent otological symptoms and the presence of retroauricular granulation tissue or and the external auditory meatus. The biopsy with characteristic histological discoveries and immunohistochemistry positive for CD1a were diagnostic. The chemotherapy may be the initial treatment in most cases or secondary in refractory or recurrent forms.
Histiocitose de células de langerhans em otorrinolaringologia
Fernandes, Humberto de Barros,Granjeiro, Ronaldo Campos,Negreiros Júnior, Jacinto de
Arquivos Internacionais de Otorrinolaringologia , 2009,
Abstract: Introdu o: A histiocitose de células de Langerhans é um distúrbio proliferativo de células inflamatórias de etiologia desconhecida. é doen a rara da faixa pediátrica. Objetivo: Realizar revis o de literatura sobre a histiocitose de células de Langerhans, focando as manifesta es otorrinolaringológicas. Materiais e métodos: A metodologia utilizada foi consulta à base de dados on line MEDLINE, de 1966 a 2008, pesquisando a partir dos termos histiocitose de células de Langerhans, osso temporal e otorrinolaringologia. Revis o da Literatura: As manifesta es em cabe a e pesco o s o as mais frequentes e seu diagnóstico torna-se difícil uma vez que mimetiza outras doen as mais comuns vistas pelo otorrinolaringologista, como otite externa, mastoidite aguda e gengivite. A doen a no osso temporal expressa-se como otorreia de repeti o e granulomas de conduto auditivo externo ou retroauricular. A avalia o radiológica evidencia les es líticas principalmente em calota craniana, mandíbula, osso temporal e costelas. O diagnóstico definitivo é feito por biópsia através dos achados histopatológicos e detec o pela imunohistoquímica do antígeno CD1a. A principal forma de tratamento é a quimioterapia e, em menor escala, radioterapia ou cirurgia. Conclus es: Manifesta es otorrinolaringológicas devem ser suspeitadas por sintomas otológicos recorrentes e pela presen a de tecido de granula o retroauricular ou de conduto auditivo externo. A biópsia com achados histológicos característicos e imunohistoquímica positiva para CD1a s o diagnósticos. A quimioterapia pode ser o tratamento inicial na maioria dos casos ou adjuvante nas formas refratárias ou recorrentes.
Unveiling novel genes upregulated by both rhBMP2 and rhBMP7 during early osteoblastic transdifferentiation of C2C12 cells
Juan C Bustos-Valenzuela, Andre Fujita, Erik Halcsik, Jose M Granjeiro, Mari C Sogayar
BMC Research Notes , 2011, DOI: 10.1186/1756-0500-4-370
Abstract: BMPs (bone morphogenetic proteins) are members of the TGFβ (transforming growth factor-β) super-family of proteins, which regulate growth and differentiation of different cell types in various tissues, and play a critical role in the differentiation of mesenchymal cells into osteoblasts. In particular, rhBMP2 and rhBMP7 promote osteoinduction in vitro and in vivo, and both proteins are therapeutically applied in orthopaedics and dentistry.Using DNA microarrays and RT-qPCR, we identified both previously known and novel genes which are upregulated by rhBMP2 and rhBMP7 during the onset of osteoblastic transdifferentiation of pre-myoblastic C2C12 cells. Subsequent studies of these genes in C2C12 and mesenchymal or pre-osteoblastic cells should reveal more details about their role during this type of cellular differentiation induced by BMP2 or BMP7. These studies are relevant to better understanding the molecular mechanisms underlying osteoblastic differentiation and bone repair.Bone formation and fracture repair depends on the expression and action of the bone morphogenetic proteins (BMPs), which are members of the transforming growth factor beta (TGF-beta) superfamily of dimeric, disulphide-linked growth factors, comprising more than 15 related proteins. In addition to a crucial role in osteogenesis, BMPs display a myriad of roles in cell proliferation, differentiation, migration and apoptosis, in different cell types [1]. Their role is essential at early phases of development and organogenesis, such as axial embryo determination [2], as well as in limb, eye and kidney development, such that ablation of these genes results in death at very early stages of development, as observed in knock-out mice [3]. In humans, recombinant BMP2 and BMP7 have gained attention in bone repair and in non-union spinal fractures due to their capacity to stimulate the differentiation of mesenchymal stem cells from the periosteum near the lesion site after migration and proliferation induced
The rabbit as an animal model for experimental surgery
Calasans-Maia, M?nica Diuana;Monteiro, Maria Lucia;áscoli, Fábio Oliveira;Granjeiro, José Mauro;
Acta Cirurgica Brasileira , 2009, DOI: 10.1590/S0102-86502009000400014
Abstract: the white new zealand rabbit (oryctolagus cuniculus) is frequently used as a model for in vivo studies. however, information on precautions when using this animal as an experimental model is limited. this review of the literature covers the gamut from the selection of the animal model all the way to its death, and describes procedures for transporting, raising, breeding, housing, administering anesthesia and handling so as to rationalize the utilization of this species while exploiting its unique characteristics. based upon the literature and our own experience with white new zealand rabbits, we conclude that the rabbit is an adequate model for experimental surgery.
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