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Search Results: 1 - 10 of 399778 matches for " J. K. Oloke "
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Comparative Study of the Degree of Severity of Hepatitis B in Preicteric, Icteric and Posticteric Hbsag Seropositive Patients Using ALT, AST, Cu, Fe, Mn, Se, Mg, Zn and Albumin
Mathew Folaranmi OLANIYAN,J.K. Oloke
Global Journal of Health Science , 2010, DOI: 10.5539/gjhs.v2n1p31
Abstract: Jaundice is the yellowish pigmentation of sclera of eye, skin and body fluids. It is associated with hepatitis B virus infection due to the destruction of the infected hepatocytes and intrahepatic-cholestasis caused by the scars of the healed hepatocytes. Hepatitis B patients that have never had jaundice are referred to as preicteric patients, while those that have recovered from jaundice are referred to as posticteric hepatitis patients. Those ones suffering from jaundice are referred to as icteric patients. This research work was designed to determine and compare the severity of hepatitis B using the serum levels of liver enzyme, albumin and micronutrients in HBsAg seropositive patients. One hundred and fifty HBsAg seropositive critically ill rural patients of the Atisbo/Saki-East/Saki-West federal constituency aged 5- 79 years; (75 females: 75males) classified into pre-icteric, icteric and posticteric patients were successfully monitored and investigated. One hundred and twelve HBsAg seronegative and HBV non-infected apparently healthy volunteers from the same community aged 4-80 years were studied as normal control volunteers. Forty three HBsAg seropositive patients were recruited from Ibadan as urban reference. The presence of hepatitis B surface antigen (HBsAg) and envelope antibodies to hepatitis B, (anti-HBe) were determined in the test and control subjects serologically (ELIZA). Similarly, the serum levels of liver enzymes Alanine-aminotransferase (ALT), and Aspartate-aminotransferase (AST) and micronutrients (Cu, Zn, Fe, Mn, Mg and Se) were biochemically (spectrophotometry and atomic absorption spectrophotometry) determined. Liver enzymes and albumin assays were routinely employed to recruit HBsAg seropositive patients in active liver destruction. Antibody to envelope antigen was used to detect patients that are infected but have been cleared of the antigens(HBsAg and HBeAg). The results obtained showed a significantly higher mean serum values of AST,ALT,Fe,Cu and Mn with a significantly lower serum albumin, Se,Mg,and Zn levels in patients compared with the results obtained from the controls during the 1st bleeding with P<0.05. There were significantly higher mean serum values of AST,ALT,Fe,Cu and Mn and significantly lower serum albumin, Se and Zn levels in patients compared with the results obtained from the control during the 2nd bleeding with P<0.05. A significantly higher mean serum levels of ALT, AST, Cu, Fe and Mn with a lower significantly mean serum levels of albumin, Se, and Zn was obtained in the patients than the control (P<0.05).
Constructional features of a 15-litre home-made bioreactor for fed-batch fermentations
Gueguim Kana E.B., J.K., Oloke, A. Lateef, M.G. Zebaze Kana
African Journal of Biotechnology , 2003,
Abstract: A 15-litre bench-top multipurpose bioreactor was designed and constructed. The vessel is a glass type with a stainless flat headplate incorporating 9 access ports allowing for a variety of interchangeable probes and actuators. The stirring speed ranges between 0 and 250 rpm, the aeration rate (0-2 l/m), the pH control loop uses HI 1131 probe, two 100 ml bottles of HCl and NaOH solutions and operates a close feedback system. The temperature control module is a close loop using a PT 100 RTD thermocouple and an auxiliary vessel containing a cooling solution. The aeration and feed flow rates are open loops. The system incorporates attributes of a good bioreactor design as discussed by Naraendranathan (1998). Sterility is achieved by autoclaving different units of the system. This machine has been tested on an array of local standard fermentation processes. (African Journal of Biotechnology: 2003 2(8): 233-236)
Akanni E.O, Oloke J.K & Fakunle E.E
International Journal of Research and Reviews in Applied Sciences , 2010,
Clastogenicity Potential Screening of Pleurotus pulmonarius and Pleurotus ostreatus Metabolites as Potential Anticancer and Antileukaemic Agents Using Micronucleus Assay
E.O. Akanni,J.K. Oloke,V.O. Mabayoje,G.O. Saka
British Journal of Pharmacology and Toxicology , 2010,
Abstract: Development of anticancer agents that will selectively destroy cancer cells without injury to normal cells has led to the discovery of novel immunotherapeutic agents such as Pleurotus pulmonarius and Pleurotus ostreatus metabolites. This study is to screen the agents of dreadful side effects of causing mutation after a prolonged use. Clastogenicity potential of the novel anti-cancer and antileukaemic agents Pleurotus pulmonarius and Pleurotus ostreatus metabolites was evaluated in this study. Wister rats were grouped into four with the test groups inoculated intraperitoneally at doses 64 and 16 mg/kg as 12.8 and 3.2% of the LD50 into the high and low dose rat groups respectively with each metabolite in a separate experiment. The treated rats were sacrificed after 24, 48 and 72 h post treatment. Cyclophosphamide (clastogen) was inoculated into the positive control group at doses 112 and 28 mg/kg w hile saline was used for the negative control group. In all the treatment groups, only the rats in the positive control group formed micronuclei in their bone marrow cells. There was only an increase in the formation of normochromatic and polychromatic erythrocytes in rat groups inoculated with Pleurotus ostreatus metabolites. There is no statistically significant difference (p>0.05) between the 3 post treatment sacrificing periods. Similar result was also obtained for Pleurotus pulmonarius group. The chromosomal damaging potential screening reveals that the Pleurotus ostreatus and Pleurotus pulmonarius metabolites are not clastogenic (genotoxic) that is, unlikely to cause cancer producing mutations, but rather enhanced erythropoiesis. They could therefore be useful anticancer agents when the potential is fully explored.
Comparative Study of the Haemagglutination Capabilities of Lectin Extracted from Submerged Cultures of Wild and Mutant Strains of Schizophyllum commune  [PDF]
O. A. Awoyinka, D. A. Aina, J. K. Oloke, O. N. Majolagbe, O. I. Akoni
Open Access Library Journal (OALib Journal) , 2014, DOI: 10.4236/oalib.1100993
Abstract: Haemagglutination studies on lectin from both wild (SCW) and mutant strains (SCM1, SCM2, SCM3) of Schizophyllum commune using human blood were explored in this study after six days of submerged fermentation. Haemagglutination assay showed that the lectin from all the strains showed slight discrimination in their haemmaglutinating activity against human blood group with strong exhibition of agglutination with blood group O while SCM3 and SCW showed the highest and lowest haemaglutinating activity respectively with a titre score from 4 - 256 HA. Absolute loss of haemagglutination activity was shown by all the four S. commune strains tested following exposure to CuSO4 and NH4SO4 at 800 mM but optimized by KCl, MgCl2 at 100 mM. Optimal pH for maximal haemagglutination activity was observed at 7.0 for SCW, 6.0 for SCM1 and 8.0 for SCM2 while SCM3 distinctly showed 5.5 and 8.5. Except for SCM3 the thermo stability of haemagglutinating activity was found to improve with the duration of UV irradiation. Inhibitory study in the presence of sugar showed that the partially purified protein from all the strains of S. commune in this study was mannose dependent lectin.
Immunomodulating properties of Trino IB immunobooster
JK Oloke
African Journal of Biotechnology , 2008,
Abstract: Trino IB mixture at a very minimal concentration (0.2 ml) was found not only to protect rats against lethal dose of pathogenic isolates of Escherichia coli and Staphylococcus aureus but it is also capable of ameliorating the deleterious effect of very high concentration of sodium arsenite (6 mg/kg body weight) in rat and bird.
Rural and Urban Planning in Developing Countries: Site Selection for Disposal of Water Treatment Residuals Using Geographic Information Sysyems (GIS)
J.O. Oluwoye,D.A. Oloke,O.I. Akinmoladun
Environmental Research Journal , 2012,
Abstract: The study proposes a conceptual framework for site selection disposal of water treatment residuals in developing countries using geographic information systems. The conceptual framework comprises 3 methodological factors, namely selection of case studies, database development and management and analysis of data and presentation of results or 4 stages, namely raw data, parameter maps, processing and final product. It is envisaged that this theoretical model provides a useful tool for rural and urban planner s practitioners in developing a more comprehensive overall planning strategies.
Novel feeding strategies for Saccharomyces cerevisiae DS2155, using glucose limited exponential fedbatch cultures with variable specific growth rates (m)
JKA Lateef, EB Oloke, G kana
African Journal of Biotechnology , 2007,
Abstract: The dual behavior of Saccharomyces cerevisiae on glucose feed as function of the dilution rate near the critical specific growth rate (ì=0.25) is a bottleneck in industrial production, hence the need for more efficient feeding strategies. In this work novel feeding strategies have been generated and evaluated. For each feeding profile, the 24 h fermentation time was discretized into 7 intervals of 6 then 3 h each. Seven values of ì were selected and fed into the feed flow rate equation, at different time intervals, in a way to maintain a pseudo exponential trend. Biopro_optimizer, the monitoring and controlling software of the 4-litre bioreactor used, generated nine different feeding strategies for evaluation in fermentation processes. The software also ensured the updating of the feed flow rate every 5 min for 24 h. The control setpoints of the bioreactor were 30°C, 4.5, 200 rpm and 1 vvm for the temperature, pH, agitation and aeration, respectively. The feeding profile with a m sequence of 0.15, 0.17,0.19 ,0.17, 0.16,0.13 and 0.11 which showed an oscillating exponential increase in feed flow rate emerged among others with a productivity of 1.80 g/h or a final concentration of 14.25 g/l from an initial of 0.3 g/l. The obtained data raise the idea of higher biomass productivity of S. cerevisiae in a multiphase strategy with an exponentially oscillating feeding profile.
Effect of nitrogen and phosphate limitation on utilization of bitumen and production of bitu-oil and gas by a bacterial consortium
JK Oloke, EA Adebayo, DA Aina
African Journal of Biotechnology , 2009,
Abstract: Five strains of bacteria; Pseudomonas fragi, Streptococcus zymogenes, Pseudomonas aeruginosa, Pseudomonas fluorescens and Bacillus macerans, capable of utilizing bitumen as source of carbon and energy were isolated from water sample obtained from bitumen producing area, Agbabu, Ondo state, Nigeria. The degradation of bitumen was found to be associated with the production of carbon (IV) oxide, natural gas and oil. As a result of using nitrogen limited and phosphate limited media, 1750 and 1250 cm3 of gas and 0.95 and 0.85 g/l of oil were obtained respectively. Nitrogen and phosphate limitation have profound effect on bitu-oil and gas production.
Antimicrobial resistance of bacterial strains isolated from orange juice products
A Lateef, JK Oloke, EB Gueguim-Kana
African Journal of Biotechnology , 2004,
Abstract: Forty samples of twenty brands of sachet orange juice products were examined microbiologically. All the products were contaminated with bacteria and yeasts. The organisms encountered include Saccharomyces cerevisiae, Saccharomyces sp, Rhodotorula sp, Bacillus cereus, Bacillus subtilis, Escherichia coli, Staphylococcus aureus, Streptococcus pyogenes and Micrococcus sp. The resistances of thirty bacterial strains isolated from orange juice products to the commonly used antibiotics were studied. About 66.67% of the isolates were resistant to augmentin and amoxycillin; 63.33% to cotrimoxazole, 56% to cloxacillin, and 23.33% to tetracycline. Resistances of 10, 6.67, and 3.33% were obtained for gentamicin, erythromycin and chloramphenicol respectively. Among the eight antibiotics tested, seven patterns of drug resistance were obtained. Six out of these are multiple-drug resistance with number of antibiotics ranging between 2 to 8. While MIC of amoxycillin ranged between 10-25mg/ml for the strains of E. coli, MIC of 10-20mg/ml was obtained for the strains of S. aureus. The MIC for cloxacillin was 0.1-1.0mg/ml for E. coli strains, and 0.01-1.0mg/ml for S. aureus strains. In all, ten strains of the bacterial isolates had evidence for the production of β-lactamases.
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