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Search Results: 1 - 10 of 1177 matches for " Izabel Yoko; "
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The effect of chlorhexidine on plaque index and mutans streptococci in orthodontic patients: A pilot study  [PDF]
Gisele Faria, Milton Santamaria Jr., Bianca Mota dos Santos, Izabel Yoko Ito, Janete Cinira Bregagnoloq, Maria Bernadete Sasso Stuani
Open Journal of Stomatology (OJST) , 2013, DOI: 10.4236/ojst.2013.36054

Aim: The purpose of this study was to assess chlorhexidine effects on plaque index and salivary levels of mutans streptococci (MS) when used as the immersion solution for removable orthodontic appliances and added to their acrylic resin composition. Methods: Forty-five patients (6 to 12 years old) were randomly assigned into three groups with 15 patients each. Group I (control)without orthodontic appliances disinfection; Group IIremovable orthodontic appliances which had been immersed in 0.12% chlorhexidine digluconate overnight (8 hours), and Group IIIorthodontic appliances in which 0.12% chlorhexidine digluconate solution had been incorporated into their resin composition. Saliva was collected for quantification of MS and evaluation of plaque index was performed before and after installation of orthodontic appliance at 0, 2, 4, 6, 8, and 10 weeks. Data were analyzed by using analysis of variance. Results: Number of MS colonies in saliva and plaque index showed no statistically differences among groups at the different periods (p > 0.05). Conclusions: It could be concluded that chlorhexidine incorporation into the acrylic resin of removable orthodontic appliances at 0.12% concentration and immersion of the appliance into 0.12% chlorhexidine solution were not effective in reducing plaque index and the

DNA Checkerboard Method for Bacterial Pathogen Identification in Oral Diseases
Nascimento,Cássio do; Issa,Jo?o Paulo Mardegan; Watanabe,Evandro; Ito,Izabel Yoko;
International Journal of Morphology , 2006, DOI: 10.4067/S0717-95022006000500017
Abstract: this work aim to show by literature review the principal characteristics of the dna checkerboard method for bacterial pathogens identification in oral diseases, showing the most varieties uses and applications of this technique
Antibacterial effect of root canal preparation and calcium hydroxide paste (Calen) intracanal dressing in primary teeth with apical periodontitis
Faria, Gisele;Nelson-Filho, Paulo;Freitas, Aldevina Campos de;Assed, Sada;Ito, Izabel Yoko;
Journal of Applied Oral Science , 2005, DOI: 10.1590/S1678-77572005000400007
Abstract: the aim of this study was to evaluate the antibacterial action of root canal mechanical preparation using 2.5% sodium hypochlorite as the irrigating solution and a calcium hydroxide paste as the antibacterial intracanal dressing in human primary teeth root canals with pulp necrosis and apical periodontitis by means of microbial culture. a total of 26 root canals of human primary teeth with pulp necrosis and apical periodontitis were used. samples were collected before, 72h after biomechanical treatment and 72h after removal of the intracanal dressing. comparison by wilcoxon test showed that root canal mechanical preparation effectively eliminated all microorganisms in 20% of the root canals, and the intracanal dressing in 62.5%; however, the cumulative action of biomechanical treatment and intracanal dressing eliminated the microorganisms of 70% of the root canals (p<0.001). isolated root canal mechanical preparation presented poorer microbiological results that those obtained with root canal mechanical preparation and the use of an intracanal dressing indicating the necessity of topical application of an intracanal medication between sessions in primary teeth with pulp necrosis and apical periodontitis.
Streptococcus mutans counts in plaque adjacent to orthodontic brackets bonded with resin-modified glass ionomer cement or resin-based composite
Mota, Solange Machado;Enoki, Carla;Ito, Izabel Yoko;Elias, Ana Maria;Matsumoto, Mírian Aiko Nakane;
Brazilian Oral Research , 2008, DOI: 10.1590/S1806-83242008000100010
Abstract: this study investigated the number of streptococcus mutans cfu (colony forming units) in the saliva and plaque adjacent to orthodontic brackets bonded with a glass ionomer cement - gic (fuji ortho) or a resin-based composite - rc (concise). twenty male and female patients, aged 12 to 20 years, participated in the study. saliva was collected before and after placement of appliances. plaque was collected from areas adjacent to brackets and saliva was again collected on the 15th, 30th, and 45th day after placement. on the 30th day, 0.4% stannous fluoride gel was applied for 4 minutes. no significant modification in the number of streptococcus mutans cfu in saliva was observed after placement of the fixed orthodontic appliances. on the 15th day, the percentage of streptococcus mutans cfu in plaque was statistically lower in sites adjacent to gic-bonded brackets (mean = 0.365) than in those adjacent to rc-bonded brackets (mean = 0.935). no evidence was found of a contribution of gic to the reduction of cfu in plaque after the 15th day. topical application of stannous fluoride gel on the 30th day reduced the number of cfu in saliva, but not in plaque. this study suggests that the antimicrobial activity of gic occurs only in the initial phase and is not responsible for a long-term anticariogenic property.
Anticariogenic effect of fluoride-releasing elastomers in orthodontic patients
Miura, Karina Katsue;Ito, Izabel Yoko;Enoki, Carla;Elias, Ana Maria;Matsumoto, Mírian Aiko Nakane;
Brazilian Oral Research , 2007, DOI: 10.1590/S1806-83242007000300007
Abstract: this in vivo experimental study evaluated the efficacy of fluoride-releasing elastomers in the control of streptococcus mutans levels in the oral cavity. forty orthodontic patients were recruited and divided into two groups of 20. fluoride-releasing elastomeric ligature ties (fluor-i-ties, ortho arch co. inc., usa) were used in the experimental group, and conventional elastomeric ligature ties (d. morelli, brazil), in the control group. two initial samples of saliva were collected at a 14-day interval to determine the number of colony forming units (cfu) of streptococcus mutans. immediately after collecting the second sample, fluoride-releasing elastomeric ligature ties were placed in the patients of the experimental group, and conventional ligature ties, in the patients of the control group. seven, 14 and 28 days after placement of the elastomeric ligature ties, saliva and plaque surrounding the orthodontic appliance were collected for microbiologic analysis. there were no significant differences in the number of streptococcus mutans cfus in saliva or plaque in the area surrounding the fluoride-releasing or conventional elastomeric ligature ties. thus, fluoride-releasing elastomeric ligature ties should not be indicated to reduce the incidence of enamel decalcification in orthodontic patients. since there was no significant reduction in s. mutans in saliva or plaque, other means of prevention against enamel decalcification should be indicated for these patients.
Evaluation of the antimicrobial activity of each component in Grossman?s sealer
Savioli, Ricardo Novak;Pecora, Jesus Djalma;Mian, Henis;Ito, Izabel Yoko;
Brazilian Oral Research , 2006, DOI: 10.1590/S1806-83242006000200007
Abstract: the antimicrobial activity of grossman?s sealer and its components was evaluated on 13 different strains using the double layer well-diffusion method. results revealed that grossman?s sealer presented antimicrobial activity against all the tested strains. among the components of the cement, sodium tetraborate presented the greatest antimicrobial activity, both in type and diameter of the halo and ring of inhibition. sealer powder, rosin, and eugenol presented similar activity, with no effect on p. aeruginosa and c. albicans. among these, only eugenol had an effect on e. coli. zinc oxide was only active against s. sobrinus and e. coli. barium sulfate and bismuth subcarbonate did not show any antimicrobial effect.
Prevalence of mutans streptococci in 93 members from six Brazilian families
PIMENTA, Fabiana Cristina;MARIN, José Moacir;UZEDA, Milton de;ITO, Izabel Yoko;
Pesquisa Odontológica Brasileira , 2001, DOI: 10.1590/S1517-74912001000300002
Abstract: several studies report that mutans streptococci (ms) are closely associated with caries in humans and that there is a correlation between the number of carious lesions and the levels of ms in the saliva of children and adults. the presence of ms in the saliva of 93 members of six brazilian families with at least 3 generations was investigated. samples of whole unstimulated saliva were collected and diluted. aliquots of 50 ml of each suspension were dropped onto sb20 agar and incubated in a candle jar at 37°c for 72h. colonies resembling ms were counted, collected, seeded in thioglycollate medium and subjected to biochemical typing. mutans streptococci were isolated from 80 subjects (86.0%) and the counts ranged from 3.0 x 102 (log 2.477) to 1.6 x 108 (log 8.204) cfu/ml of saliva. all of the 73 adults were colonized by ms, but the bacteria were detected in only 7 (35.0%) of the 20 children evaluated. streptococcus mutans occurred in 78 subjects (97.5%), and 51 (63.7%) were monocolonized. s. sobrinus occurred in 29 individuals (36.3%) and 2 (2.5%) were monocolonized. twenty-seven (33.8%) subjects were multicolonized with s. mutans and s. sobrinus. this study showed a high prevalence (86.0%) of mutans streptococci in the saliva of members of the studied families, which suggests the risk of intrafamilial transmission.
Efeito da anti-sepsia da ferida cirúrgica alveolar sobre o crescimento bacteriano em fios de sutura de algod?o
SOARES, Ulysses Nicida;ITO, Izabel Yoko;ROCHA BARROS, Valdemar Mallet da;
Pesquisa Odontológica Brasileira , 2001, DOI: 10.1590/S1517-74912001000100008
Abstract: the objective of this study was to evaluate bacterial growth on cotton suture. the efficiency of cetylpyridinium chloride (50%), hydrogen peroxide (3%) and chlorhexidine (0.12%) in antisepsis was investigated. for that, 20 patients who were submitted to extraction of impacted lower third molars were studied. five days after extraction, samples were obtained from the oral and alveolar sides of the sutures, before and after antisepsis of the wounds, and were submitted to bacteriological analysis. bacterial growth was observed in all examined samples. the number of streptococci decreased after antisepsis and there were no statistically significant differences between the methods of antisepsis used.
AZEVEDO, Rosa Vitória Palamin;NELSON FILHO, Paulo;ASSED, Sada;ITO, Izabel Yoko;
Revista de Odontologia da Universidade de S?o Paulo , 1998, DOI: 10.1590/S0103-06631998000100008
Abstract: the aim of this study was to determine the prevalence of species of the mutans group in samples of non-stimulated saliva from 50 children and their mothers. aliquots of pure saliva were submitted to seriate decimal dilution, plated with the aid of the l glass rod onto sb20 modified medium, and incubated in a candle jar for 72 hours at 37oc. after the count of the cfu of the mutans group, the characteristic colonies were transferred to thioglycolatte medium, incubated for 24hours at 37oc and submitted to a biochemical identification scheme. streptococci of the mutans group were detected in the saliva of 47 (94.0%) children, and in 50 (100.0%) mothers. s. mutans and s. sobrinus were detected in 15 (30.0%) children, and in 24 (48.0%) mothers who were multicolonized, with the cfu number corresponding to 1.28 x 106 and 3.57 x 106, respectively. the same species of the mutans group were harbored by 31 (62.0%) mother-child pairs.
DNA Checkerboard Method for Bacterial Pathogen Identification in Oral Diseases Método de Chequeo de DNA para la Identificación de Bacterias Patógenas en las Enfermedades de la Cavidad Oral
Cássio do Nascimento,Jo?o Paulo Mardegan Issa,Evandro Watanabe,Izabel Yoko Ito
International Journal of Morphology , 2006,
Abstract: This work aim to show by literature review the principal characteristics of the DNA checkerboard method for bacterial pathogens identification in oral diseases, showing the most varieties uses and applications of this technique Este trabajo tiene como objetivo, presentar en una revisión de la literatura, las principales características del método de chequeo del DNA para la identificación de bacterias patógenas en la cavidad oral, mostrando las diferentes utilizaciones y aplicaciones de esta técnica
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