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Search Results: 1 - 10 of 144 matches for " Ivens Cuiabano Scaff "
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Prevalence of Human Leukocyte Antigen HLA-B*5701 in HIV-1 Infected Individuals in Brazil  [PDF]
Claudinéia de Araújo, Cristina Valetta de Carvalho, Miriam Estela de Souza Freire, Amanda Yamaguti, Ivens Cuiabano Scaff, Fernando José de Souza, Flávia Galindo Silvestre Silva, Ricardo Sobhie Diaz, Ismael Dale Cotrim Guerreiro da Silva
Open Journal of Genetics (OJGen) , 2014, DOI: 10.4236/ojgen.2014.41008

This study was designed to establish the prevalence of HLA-B*5701 at HIV-1 infected individuals in Brazil. A total of 517 consecutive individuals were followed in this study from February 2009 through July 2011. The presence of HLA-B*5701 was determined by Nested-PCR with HLA-B*57 and HLA-B*5701 sequence-specific primers (PCR-SSP). The expression of HLA-B*57 was negative in the 385 (74.5%) and positive in the 103 (19.9%) of infected individuals. Among these, the expression of HLA-B5701 was positive in the 29 (5.6%) of individuals. No demographic or ethnic differences were found between HLA-B*57/HLA-B*5701 HIV-1 negative patients, with a prevalence of Caucasians (57.1%) individuals. During the period of study, 68 patients were submited to an abacavir contain- ing regimen. The HLA-B*5701 allele was observed in 7 (10.3%) patients, with a significant incidence of Hypersensitivity reactions at 4 of them (p < 0.001). Conclusions: Although Brazilian population consists of a mixture of individuals of Caucasian, African and Native American genetic background, prevalence of HLA-B*5701 in this population is similar to the one found in pure Caucasians.

A software framework for microarray and gene expression object model (MAGE-OM) array design annotation
Matloob Qureshi, Alasdair Ivens
BMC Genomics , 2008, DOI: 10.1186/1471-2164-9-133
Abstract: We have developed a graphical Java-based application (Adamant) to assist with submission of Microarray designs to public repositories. Output of the application is fully compliant with the standards prescribed by the various public data repositories.Adamant will allow researchers to annotate and submit their own array designs to public repositories without requiring programming expertise, knowledge of the MAGE-OM or XML. The application has been used to submit a number of ArrayDesigns to the Array Express database.Genome-wide measurement of gene expression and genomic variation via DNA microarrays is a widely used technique in biological and medical research. The quantity and complexity of data generated by such studies represents a challenge in terms of storage, analysis and data transfer. There are a number of different microarray technologies and platforms which generate data in a range of different formats and units. The Microarray Gene Expression Data (MGED) society has proposed a standard known as the Minimum Information About a Microarray Experiment (MIAME) standard to address these issues [1]. A MIAME-compliant data-centric model has been developed: the Microarray Gene Expression Object Model (MAGE-OM); this applies a structure to all components of a microarray-based study, from the sequences attached to the array through to describing equipment and platform used to perform the hybridisation reactions [2]. The MGED Ontology (MO) provides a common terminology and hierarchy for data annotation within the MAGE-OM [3]. A notable aspect of the MO is that it allows terms from existing external ontologies to be referenced within the MO framework. Additionally, an XML data-interchange format, MAGE mark-up language, MAGE-ML, has been derived from the MAGE-OM. The development of MAGE-ML facilitates the organisation and transfer of microarray data between databases and a range of analysis programs [2].There are three public repositories of data produced from microarray
Uso do Doppler transcraniano para monitoriza??o do vasoespasmo cerebral secundário à hemorragia subaracnóide
Radanovic, M.;Scaff, M.;
Revista da Associa??o Médica Brasileira , 2001, DOI: 10.1590/S0104-42302001000100031
Abstract: cerebral vasospasm accounts for an increased morbidity and mortality in patients with spontaneous subarachnoid hemorhage (sah). its early and non invasive detection by transcranial doppler (tcd) may contribute to a better prognosis in such cases. purpose: to discuss the use of transcranial doppler (tcd) in the detection of cerebral vasospasm secondary cerebral to spontaneous subarachnoid hemorrhage (sah). methods: we analyzed the flow velocities registered by tcd in intracranial arteries in 31 patients with sah, performed sequentially, associated with neurological examinations. all patients (except one) had intracranial aneurysms. results: clinical vasospasm was found in 11 patients (36,6% from the total), with correspondent sonographic vasospasm present in 9 cases (82%). in 3 cases (33.3%) the flow velocity alterations preceded the clinical symptoms. between the 20 asymptomatic patients, 15 (75%) had normal flow velocity, with evidence of sonographic vasospasm without clinical repercussion in 5 (25%). the index of false negatives in the correlation between clinical examination and tcd (symptomatic vasospasm with normal flow velocity records) was 18%. however, there was correspondence between clinical and ultrasonographic findings in 26 of the 31 patients (83.8%). the average flow velocity values in the various arteries and its interpretation for each subgroup are discussed with further details. conclusion: tcd is a valuable auxiliary method in the detection of cerebral vasospasm (even pre-symptomatic) secondary to sah, allowing a more precocious therapeutic intervention. our results are similar to those described in the literature.
Uso do Doppler transcraniano para monitoriza o do vasoespasmo cerebral secundário à hemorragia subaracnóide
Radanovic M.,Scaff M.
Revista da Associa??o Médica Brasileira , 2001,
Abstract: O vasoespasmo cerebral é responsável por aumento de morbidade e mortalidade em pacientes acometidos por hemorragia subaracnóide (HSA). Sua detec o de forma precoce e n o-invasiva pelo método de Doppler transcraniano (DTC) pode contribuir para um melhor prognóstico destes doentes. OBJETIVO: Discutir o uso do método de DTC para diagnóstico do vasoespasmo cerebral secundário à HSA espontanea. MéTODOS: Foram analisados os registros de velocidade de fluxo em artérias intracranianas por DTC em 31 pacientes com diagnóstico tomográfico de HSA, realizados seqüencialmente em conjunto com exame neurológico. Todos os pacientes (à exce o de um) eram portadores de aneurismas intracranianos. RESULTADOS: Vasoespasmo clínico foi constatado em 11 pacientes (36,6% do total), com correspondente vasoespasmo ultra-sonográfico presente em nove casos (82%). Em três casos (33,3%) as altera es de velocidade de fluxo precederam os sinais clínicos. Entre os 20 pacientes assintomáticos, 15 (75%) apresentaram medidas de velocidade normais, havendo evidência de vasoespasmo sonográfico sem repercuss o clínica em cinco (25%). O índice de falso negativo na correla o entre exame clínico e DTC (sinais clínicos de vasoespasmo presentes, com velocidades de fluxo normais) foi de 18%. No entanto, houve correspondência entre os achados clínicos e ultra-sonográficos em 26 dos 31 pacientes (83,8%). Os valores de velocidades médias nas várias artérias e sua interpreta o para cada subgrupo s o discutidos com maiores detalhes. CONCLUS O: O método de DTC é um auxiliar valioso na detec o pré-sintomática do vasoespasmo secundário à HSA, permitindo uma interven o terapêutica mais precoce. Os dados obtidos neste trabalho coadunam-se com os diversos estudos publicados na literatura.
Os novos bacharéis em direito no Brasil no início do século XXI: primeiras considera es
Fernando Facury Scaff
Scientia Iuris , 2000,
Abstract: The article refers to the public politics of the education like serviceable to law graduate in public and private institution in the twenty one century. O artigo refere-se à política pública da educa o como prestador de servi o aos bacharéis de direito em institui es públicas e privadas no século XXI.
Aduba??o mineral para o trigo no sul do estado de S?o Paulo
Blanco, H. Garcia;Venturini, W. R.;Gargantini, H.;Cuiabano, Ney;
Bragantia , 1965, DOI: 10.1590/S0006-87051965000100036
Abstract: this paper reports about several factorial tests carried out in 1960 and with n p k fertilization for wheat on ortho dark red latosol in the south of the state of s?o paulo, occupied with field vegetation. the results showed that these soils with over 0.10%of total nitrogen did not react to nitrogenated fertilizers, whereas 0.10 e.mg of potassium was the limit of soil reaction. phosphorus was the element reacting the most showing an evident correlation between response to fertilization and soluble content of the element in the soil. for soils with amounts equal to 0.18 e.mg. of po4-3 soluble in h2so4 0.05 n, productions of around 1000 kg per hectare of wheat seeds were reached without any addition of posphorous fertilizers. at this level, any addition of p2o5 implies to an increase of yield. the results indicate further that the fertility indexes established by catani and others, for the soils of the state of s?o paulo do not apply to the ortho dark red latosol when grown with wheat, concerning the elements nitrogen and potassium.
Vertigem de posicionamento paroxística benigna
Pereira, Cristiana Borges;Scaff, Milberto;
Arquivos de Neuro-Psiquiatria , 2001, DOI: 10.1590/S0004-282X2001000300031
Abstract: benign paroxysmal positioning vertigo is a frequent vestibular disorder. with a simple maneuver one can easily diagnose this condition and treatment is based on liberatory maneuvers. on this review the pathogenesis, the clinical features and different maneuvers are briefly discussed.
Morphological and histochemical analysis of the antennal gland of Macrobrachium Potiuna (Muller, 1880) (Crustácea, Decapoda, Palaemonidae)
Moraes, Neci;Scaff, Rejane Cirra;
Brazilian Archives of Biology and Technology , 1998, DOI: 10.1590/S1516-89131998000300005
Abstract: the objective of our study was to describe by histologic methods the structural organization of the antennal gland of m. potiuna as well as the location through histochemical methods of the neutral and sulfated polissacharides that exist in these regions. this study showed that the gland is composed of the a non-secretive tubular portion and a secretive portion which is formed by a cubic simple epithelium, sub-epithelium layer and cellular chains
Forma o de professores da educa o básica: avan os e desafios das políticas recentes = Teacher training for basic education: advances and challenges of recent policies
Elisangela Alves da Silva Scaff
Linhas Críticas , 2012,
Abstract: O presente texto tem como objeto o Plano Nacional de Forma o de Professores (PARFOR), no que diz respeito aos cursos de forma o inicial. Realiza uma análise do referido plano buscando descrever e analisar sua implanta o e implementa o no estado de Mato Grosso do Sul e tendo como principais fontes os documentos oficiais de ambito nacional, informa es do site eletr nico que gerencia o processo de inscri o e sele o ao PARFOR – Plataforma Freire, dados disponibilizados pelas Institui es envolvidas no plano e pelo Fórum Permanente de Apoio à Forma o Docente em Mato Grosso do Sul.This paper approaches the Brazilian teacher training plan (PARFOR), in what refers to initial training courses. It studies the plan, attempting to describe and analyze its implantation and implementation in the state of Mato Grosso do Sul. The main sources for this study are the official national-level documents, information from the website that manages the registration and selection process of the PARFOR – the Plataforma Freire, and information shared by the institutions involved in the plan and by the permanent Forum of support to teacher training of the state of Mato Grosso do Sul.
Vertigem de posicionamento paroxística benigna
Pereira Cristiana Borges,Scaff Milberto
Arquivos de Neuro-Psiquiatria , 2001,
Abstract: A vertigem de posicionamento paroxística benigna é um distúrbio vestibular de diagnóstico fácil, baseado no exame neurológico e tratado com manobras de libera o. Nesta revis o, a fisiopatologia, os aspectos clínicos e as diferentes manobras s o sucintamente discutidos.
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